Restriction map for pF1KSDA1720
 (Linear) (Six-base) MAPSORT of: KIAA1720.seq  Check: 5334  from: 1  to: 1179



KIAA1720.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80    1010    1179 
   Size:         80     930     169

  Fragments arranged by size: 

                 930     169      80
 
AfeI AGC'GCT

Cuts at:      0     401    1179 
   Size:        401     778
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     452    1179 
   Size:        452     727
 
AlwNI CAG_nnn'CTG

Cuts at:      0     705    1179 
   Size:        705     474
 
ApaI G_GGCC'C

Cuts at:      0     856     971    1179 
   Size:        856     115     208

  Fragments arranged by size: 

                 856     208     115
 
AvaI C'yCGr_G

Cuts at:      0     440     617     892    1179 
   Size:        440     177     275     287

  Fragments arranged by size: 

                 440     287     275     177
 
AvrII C'CTAG_G

Cuts at:      0      82    1179 
   Size:         82    1097
 
BamHI G'GATC_C

Cuts at:      0     168     269    1179 
   Size:        168     101     910

  Fragments arranged by size: 

                 910     168     101
 
BanI G'GyrC_C

Cuts at:      0     821    1121    1157    1179 
   Size:        821     300      36      22

  Fragments arranged by size: 

                 821     300      36      22
 
BanII G_rGCy'C

Cuts at:      0      11      91     683     856     971    1146    1179 
   Size:         11      80     592     173     115     175      33

  Fragments arranged by size: 

                 592     175     173     115      80      33      11
 
BbeI G_GCGC'C

Cuts at:      0     825    1125    1179 
   Size:        825     300      54

  Fragments arranged by size: 

                 825     300      54
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     643    1179 
   Size:        643     536
 
BbvCI CC'TCA_GC

Cuts at:      0     611    1179 
   Size:        611     568
 
BlpI GC'TnA_GC

Cuts at:      0       5     377     461    1006    1179 
   Size:          5     372      84     545     173

  Fragments arranged by size: 

                 545     372     173      84       5
 
Bme1580I G_kGCm'C

Cuts at:      0     856     971    1179 
   Size:        856     115     208

  Fragments arranged by size: 

                 856     208     115
 
BmgBI CAC'GTC

Cuts at:      0    1080    1179 
   Size:       1080      99
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      85     684    1179 
   Size:         85     599     495

  Fragments arranged by size: 

                 599     495      85
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     727     757     811     967    1179 
   Size:        128     599      30      54     156     212

  Fragments arranged by size: 

                 599     212     156     128      54      30
 
Bpu10I CC'TnA_GC

Cuts at:      0     368     611     752    1179 
   Size:        368     243     141     427

  Fragments arranged by size: 

                 427     368     243     141
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     667    1179 
   Size:        667     512
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      28    1179 
   Size:         28    1151
 
BsaAI yAC'GTr

Cuts at:      0     503    1179 
   Size:        503     676
 
BsaHI Gr'CG_yC

Cuts at:      0     470     822    1122    1179 
   Size:        470     352     300      57

  Fragments arranged by size: 

                 470     352     300      57
 
BsaWI w'CCGG_w

Cuts at:      0     644    1179 
   Size:        644     535
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     125     169     199     229     724     855     964    1179 
   Size:        125      44      30      30     495     131     109     215

  Fragments arranged by size: 

                 495     215     131     125     109      44      30      30
            
 
BseYI C'CCAG_C

Cuts at:      0    1101    1179 
   Size:       1101      78
 
BsiHKAI G_wGCw'C

Cuts at:      0      11     197     581     683     716    1058    1179 
   Size:         11     186     384     102      33     342     121

  Fragments arranged by size: 

                 384     342     186     121     102      33      11
 
Bsp1286I G_dGCh'C

Cuts at:      0      11      91     197     581     683     716     856     971 
   Size:         11      80     106     384     102      33     140     115

Cuts at:    971    1058    1146    1179 
   Size:         87      88      33

  Fragments arranged by size: 

                 384     140     115     106     102      88      87      80
                  33      33      11
 
BspEI T'CCGG_A

Cuts at:      0     644    1179 
   Size:        644     535
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     538     735    1040    1179 
   Size:        538     197     305     139

  Fragments arranged by size: 

                 538     305     197     139
 
BsrBI CCG'CTC

Cuts at:      0     414     558     573    1179 
   Size:        414     144      15     606

  Fragments arranged by size: 

                 606     414     144      15
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     513    1179 
   Size:        513     666
 
BstYI r'GATC_y

Cuts at:      0     168     269     701    1179 
   Size:        168     101     432     478

  Fragments arranged by size: 

                 478     432     168     101
 
BtgI C'CryG_G

Cuts at:      0     773     825     872    1049    1146    1179 
   Size:        773      52      47     177      97      33

  Fragments arranged by size: 

                 773     177      97      52      47      33
 
BtsI GCAGTG_nn'

Cuts at:      0     135    1179 
   Size:        135    1044
 
EaeI y'GGCC_r

Cuts at:      0    1092    1179 
   Size:       1092      87
 
EarI CTCTTCn'nnn_

Cuts at:      0      95     548    1179 
   Size:         95     453     631

  Fragments arranged by size: 

                 631     453      95
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     562    1154    1179 
   Size:        562     592      25

  Fragments arranged by size: 

                 592     562      25
 
EcoICRI GAG'CTC

Cuts at:      0       9     681    1179 
   Size:          9     672     498

  Fragments arranged by size: 

                 672     498       9
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     128     727     757     811     967    1010    1179 
   Size:         80      48     599      30      54     156      43     169

  Fragments arranged by size: 

                 599     169     156      80      54      48      43      30
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     110     202     264    1179 
   Size:        110      92      62     915

  Fragments arranged by size: 

                 915     110      92      62
 
EcoO109I rG'GnC_Cy

Cuts at:      0     853     889     967    1179 
   Size:        853      36      78     212

  Fragments arranged by size: 

                 853     212      78      36
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     577    1179 
   Size:        577     602
 
FspI TGC'GCA

Cuts at:      0    1015    1179 
   Size:       1015     164
 
HaeII r_GCGC'y

Cuts at:      0     403     825     864    1125    1179 
   Size:        403     422      39     261      54

  Fragments arranged by size: 

                 422     403     261      54      39
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     246     278    1179 
   Size:        246      32     901

  Fragments arranged by size: 

                 901     246      32
 
KasI G'GCGC_C

Cuts at:      0     821    1121    1179 
   Size:        821     300      58

  Fragments arranged by size: 

                 821     300      58
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     170    1179 
   Size:        170    1009
 
MspA1I CmG'CkG

Cuts at:      0     240     827     917    1179 
   Size:        240     587      90     262

  Fragments arranged by size: 

                 587     262     240      90
 
NarI GG'CG_CC

Cuts at:      0     822    1122    1179 
   Size:        822     300      57

  Fragments arranged by size: 

                 822     300      57
 
NspI r_CATG'y

Cuts at:      0     429    1179 
   Size:        429     750
 
PasI CC'CwG_GG

Cuts at:      0     849    1179 
   Size:        849     330
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     705     849    1101    1179 
   Size:        705     144     252      78

  Fragments arranged by size: 

                 705     252     144      78
 
PfoI T'CCnGG_A

Cuts at:      0      21     704    1179 
   Size:         21     683     475

  Fragments arranged by size: 

                 683     475      21
 
PmlI CAC'GTG

Cuts at:      0     503    1179 
   Size:        503     676
 
PpuMI rG'GwC_Cy

Cuts at:      0     889    1179 
   Size:        889     290
 
PspOMI G'GGCC_C

Cuts at:      0     852     967    1179 
   Size:        852     115     212

  Fragments arranged by size: 

                 852     212     115
 
PstI C_TGCA'G

Cuts at:      0     635    1037    1179 
   Size:        635     402     142

  Fragments arranged by size: 

                 635     402     142
 
PvuII CAG'CTG

Cuts at:      0     240    1179 
   Size:        240     939
 
SacI G_AGCT'C

Cuts at:      0      11     683    1179 
   Size:         11     672     496

  Fragments arranged by size: 

                 672     496      11
 
SacII CC_GC'GG

Cuts at:      0     828    1179 
   Size:        828     351
 
SapI GCTCTTCn'nnn_

Cuts at:      0      95     548    1179 
   Size:         95     453     631

  Fragments arranged by size: 

                 631     453      95
 
SexAI A'CCwGG_T

Cuts at:      0     224    1179 
   Size:        224     955
 
SfcI C'TryA_G

Cuts at:      0     235     274     631    1033    1179 
   Size:        235      39     357     402     146

  Fragments arranged by size: 

                 402     357     235     146      39
 
SfoI GGC'GCC

Cuts at:      0     823    1123    1179 
   Size:        823     300      56

  Fragments arranged by size: 

                 823     300      56
 
SmlI C'TyrA_G

Cuts at:      0     440     682    1179 
   Size:        440     242     497

  Fragments arranged by size: 

                 497     440     242
 
SphI G_CATG'C

Cuts at:      0     429    1179 
   Size:        429     750
 
StuI AGG'CCT

Cuts at:      0    1067    1179 
   Size:       1067     112
 
StyI C'CwwG_G

Cuts at:      0      82     722    1179 
   Size:         82     640     457

  Fragments arranged by size: 

                 640     457      82
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     487     519    1179 
   Size:        487      32     660

  Fragments arranged by size: 

                 660     487      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1078    1179 
   Size:       1078     101
 
XhoI C'TCGA_G

Cuts at:      0     440    1179 
   Size:        440     739
 

 Enzymes that do cut:

     AcuI     AfeI     AlfI    AlwNI     ApaI     AvaI    AvrII    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BlpI Bme1580I    BmgBI
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BseRI    BseYI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI   BstAPI
    BstYI     BtgI     BtsI     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I     KasI     MmeI
   MspA1I     NarI     NspI     PasI    PflMI     PfoI     PmlI    PpuMI
   PspOMI     PstI    PvuII     SacI    SacII     SapI    SexAI     SfcI
     SfoI     SmlI     SphI     StuI     StyI     TstI  Tth111I     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
     BaeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BmtI
     BplI    BsaBI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI    EcoRI    EcoRV     FseI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MscI     MslI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SalI    SanDI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     SwaI    TaqII     TatI     XbaI
     XcmI     XmaI     XmnI     ZraI
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