Restriction map for pF1KSDA1719
##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

Acc65I      AccI        AcsI        AflII       AflIII      
AgeI        AocI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AviII       AvrII       
BbeI        BcgI        BciVI       BfrI        BglII       
BlnI        BsaBI       BsiWI       Bsp1407I    BspLU11I    
BsrGI       BssHII      Bst1107I    BstBI       BstZ17I     
Bsu36I      CpoI        DraI        EarI        Eco81I      
EcoRI       EcoRV       EcoT22I     FspI        HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MamI        MfeI        MluI        MseI        MunI        
NarI        NdeI        NotI        NruI        NsiI        
NspV        PacI        PinAI       PmeI        PshAI       
PshBI       RsrII       SacII       SalI        SapI        
ScaI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        SspBI       SspI        SwaI        
Tru9I       Tsp509I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII         442  GACGTC               GACGT^C
AclI         2122  AACGTT               AA^CGTT
AhdI         2635  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1348  GTGCAC               G^TGCAC
Aor51HI      1979  AGCGCT               AGC^GCT
ApaLI        1348  GTGCAC               G^TGCAC
AspEI        2635  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BspDI         898  ATCGAT               AT^CGAT
BspHI        2687  TCATGA               T^CATGA
BsrDI        1539  CATTGC               GCAATG(2/0)
BstEII       2823  GGTGACC              G^GT[ACGT]ACC
BstPI        2823  GGTGACC              G^GT[ACGT]ACC
ClaI          898  ATCGAT               AT^CGAT
EagI         2140  CGGCCG               C^GGCCG
Eam1105I     2635  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2140  CGGCCG               C^GGCCG
Eco47III     1979  AGCGCT               AGC^GCT
Eco52I       2140  CGGCCG               C^GGCCG
EcoO65I      2823  GGTGACC              G^GT[ACGT]ACC
FseI         2137  GGCCGGCC             GGCCGG^CC
HaeII        1979  AGCGCT               [GA]GCGC^[CT]
HincII       2971  GTCAAC               GT[CT]^[GA]AC
HindII       2971  GTCAAC               GT[CT]^[GA]AC
NcoI         1853  CCATGG               C^CATGG
NheI         1311  GCTAGC               G^CTAGC
NspI         2612  GCATGT               [GA]CATG^[CT]
PaeR7I       2716  CTCGAG               C^TCGAG
Psp1406I     2122  AACGTT               AA^CGTT
PvuI         3097  CGATCG               CGAT^CG
RcaI         2687  TCATGA               T^CATGA
SacI         1299  GAGCTC               GAGCT^C
SexAI        1892  ACCTGGT              A^CC[AT]GGT
SfiI         1007  GGCCACTGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         2423  CCCGGG               CCC^GGG
TfiI          611  GAATC                G^A[AT]TC
XhoI         2716  CTCGAG               C^TCGAG
XmaCI        2423  CCCGGG               C^CCGGG
XmaI         2423  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccII         364  CGCG                 CG^CG
             1593  CGCG                
AccIII        803  TCCGGA               T^CCGGA
             1910  TCCGGA              
AfaI         2761  GTAC                 GT^AC
             2984  GTAC                
BamHI         302  GGATCC               G^GATCC
             1234  GGATCC              
BbrPI        1669  CACGTG               CAC^GTG
             2092  CACGTG              
BbsI         2632  GAAGAC               GAAGAC(2/6)
             1161  GTCTTC              
BfaI         1312  CTAG                 C^TAG
             2212  CTAG                
BpuAI        2632  GAAGAC               GAAGAC(2/6)
             1161  GTCTTC              
BsaWI         803  TCCGGA               [AT]^CCGG[AT]
             1910  TCCGGA              
BseAI         803  TCCGGA               T^CCGGA
             1910  TCCGGA              
BsmBI         444  CGTCTC               CGTCTC(1/5)
             2868  CGTCTC              
BsmI          113  GCATTC               GAATGC(1/-1)
             2105  GCATTC              
BspEI         803  TCCGGA               T^CCGGA
             1910  TCCGGA              
BssSI         670  CACGAG               CACGAG(-5/-1)
             1456  CTCGTG              
BstAPI       1341  GCAGATTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1869  GCAAATCCTGC         
BstUI         364  CGCG                 CG^CG
             1593  CGCG                
DraIII       2818  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2905  CACACTGTG           
DrdI          759  GACGCCAGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2032  GACCCCATTGTC        
MaeI         1312  CTAG                 C^TAG
             2212  CTAG                
MroI          803  TCCGGA               T^CCGGA
             1910  TCCGGA              
MvnI          364  CGCG                 CG^CG
             1593  CGCG                
NaeI         2138  GCCGGC               GCC^GGC
             2322  GCCGGC              
NgoMIV       2138  GCCGGC               G^CCGGC
             2322  GCCGGC              
PflMI        1651  CCAAGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2575  CCAGCCCCTGG         
PmaCI        1669  CACGTG               CAC^GTG
             2092  CACGTG              
PmlI         1669  CACGTG               CAC^GTG
             2092  CACGTG              
PvuII        1051  CAGCTG               CAG^CTG
             2516  CAGCTG              
RsaI         2761  GTAC                 GT^AC
             2984  GTAC                
SbfI         2166  CCTGCAGG             CCTGCA^GG
             2964  CCTGCAGG            
Sse8387I     2166  CCTGCAGG             CCTGCA^GG
             2964  CCTGCAGG            
Van91I       1651  CCAAGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2575  CCAGCCCCTGG         
XcmI          938  CCACCAAGCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2057  CCAAGCGTGGCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AciI        AcyI        AluI        AlwI        AlwNI       
ApaI        AspHI       AspI        AvaI        AvaII       
BalI        BanI        BanII       BbvCI       BbvI        
BclI        BcnI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BsaAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
FbaI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflFI       PleI        PpuMI       PstI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        StyI        TaqI        TseI        Tsp45I      
TspRI       Tth111I     TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com