Restriction map for pF1KSDA1719
 (Linear) (Six-base) MAPSORT of: KIAA1719.seq  Check: 7625  from: 1  to: 3129



KIAA1719.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1140    1497    2160    3129 
   Size:       1140     357     663     969

  Fragments arranged by size: 

                1140     969     663     357
 
AatII G_ACGT'C

Cuts at:      0     446    3129 
   Size:        446    2683
 
AclI AA'CG_TT

Cuts at:      0    2123    3129 
   Size:       2123    1006
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598    1924    1996    2215    3129 
   Size:        598    1326      72     219     914

  Fragments arranged by size: 

                1326     914     598     219      72
 
AfeI AGC'GCT

Cuts at:      0    1981    3129 
   Size:       1981    1148
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2640    3129 
   Size:       2640     489
 
AleI CACnn'nnGTG

Cuts at:      0     142    3129 
   Size:        142    2987
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1609    1641    3129 
   Size:       1609      32    1488

  Fragments arranged by size: 

                1609    1488      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     769    1581    2581    3129 
   Size:        769     812    1000     548

  Fragments arranged by size: 

                1000     812     769     548
 
ApaI G_GGCC'C

Cuts at:      0      50    2395    3093    3129 
   Size:         50    2345     698      36

  Fragments arranged by size: 

                2345     698      50      36
 
ApaLI G'TGCA_C

Cuts at:      0    1348    3129 
   Size:       1348    1781
 
AvaI C'yCGr_G

Cuts at:      0    2423    2591    2716    3129 
   Size:       2423     168     125     413

  Fragments arranged by size: 

                2423     413     168     125
 
BamHI G'GATC_C

Cuts at:      0     302    1234    3129 
   Size:        302     932    1895

  Fragments arranged by size: 

                1895     932     302
 
BanI G'GyrC_C

Cuts at:      0     196     493     904    1660    1792    1951    2083    2695 
   Size:        196     297     411     756     132     159     132     612

Cuts at:   2695    3012    3129 
   Size:        317     117

  Fragments arranged by size: 

                 756     612     411     317     297     196     159     132
                 132     117
 
BanII G_rGCy'C

Cuts at:      0      14      50     574     870    1303    1745    2395    2489 
   Size:         14      36     524     296     433     442     650      94

Cuts at:   2489    2575    3093    3129 
   Size:         86     518      36

  Fragments arranged by size: 

                 650     524     518     442     433     296      94      86
                  36      36      14
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1154    2639    3129 
   Size:       1154    1485     490

  Fragments arranged by size: 

                1485    1154     490
 
BbvCI CC'TCA_GC

Cuts at:      0    1390    2461    2665    3129 
   Size:       1390    1071     204     464

  Fragments arranged by size: 

                1390    1071     464     204
 
BclI T'GATC_A

Cuts at:      0     152    1748    3050    3129 
   Size:        152    1596    1302      79

  Fragments arranged by size: 

                1596    1302     152      79
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1014    1338    1597    2135    2457    3129 
   Size:       1014     324     259     538     322     672

  Fragments arranged by size: 

                1014     672     538     324     322     259
 
BlpI GC'TnA_GC

Cuts at:      0      13     598    2146    3129 
   Size:         13     585    1548     983

  Fragments arranged by size: 

                1548     983     585      13
 
Bme1580I G_kGCm'C

Cuts at:      0      50     498     512     995    1079    1352    1795    2017 
   Size:         50     448      14     483      84     273     443     222

Cuts at:   2017    2333    2395    2431    2698    3093    3129 
   Size:        316      62      36     267     395      36

  Fragments arranged by size: 

                 483     448     443     395     316     273     267     222
                  84      62      50      36      36      14
 
BmgBI CAC'GTC

Cuts at:      0    2979    3129 
   Size:       2979     150
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2327    3129 
   Size:       2327     802
 
BmtI G_CTAG'C

Cuts at:      0    1315    3129 
   Size:       1315    1814
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1405    1944    2644    3129 
   Size:       1405     539     700     485

  Fragments arranged by size: 

                1405     700     539     485
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     394     899     997    1302    1390    1396    1636 
   Size:         50     344     505      98     305      88       6     240

Cuts at:   1636    1819    1960    2198    2659    2695    2929    3129 
   Size:        183     141     238     461      36     234     200

  Fragments arranged by size: 

                 505     461     344     305     240     238     234     200
                 183     141      98      88      50      36       6
 
Bpu10I CC'TnA_GC

Cuts at:      0    1390    2461    2665    3129 
   Size:       1390    1071     204     464

  Fragments arranged by size: 

                1390    1071     464     204
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     952    2116    3129 
   Size:        331     621    1164    1013

  Fragments arranged by size: 

                1164    1013     621     331
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      16     228     875    1351    1376    1429    1936    2176 
   Size:         16     212     647     476      25      53     507     240

Cuts at:   2176    2248    3129 
   Size:         72     881

  Fragments arranged by size: 

                 881     647     507     476     240     212      72      53
                  25      16
 
BsaAI yAC'GTr

Cuts at:      0     540    1671    2094    3129 
   Size:        540    1131     423    1035

  Fragments arranged by size: 

                1131    1035     540     423
 
BsaHI Gr'CG_yC

Cuts at:      0     443     760    1589    1862    3129 
   Size:        443     317     829     273    1267

  Fragments arranged by size: 

                1267     829     443     317     273
 
BsaWI w'CCGG_w

Cuts at:      0     803    1910    3129 
   Size:        803    1107    1219

  Fragments arranged by size: 

                1219    1107     803
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     367     397    1609    1639    2171    2201    2776    2806 
   Size:        367      30    1212      30     532      30     575      30

Cuts at:   2806    3129 
   Size:        323

  Fragments arranged by size: 

                1212     575     532     367     323      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     136     503    1056    1287    2551    2554    2557    2611 
   Size:        136     367     553     231    1264       3       3      54

Cuts at:   2611    2800    3129 
   Size:        189     329

  Fragments arranged by size: 

                1264     553     367     329     231     189     136      54
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0     174    1061    1216    1238    1701    2277    2514    2574 
   Size:        174     887     155      22     463     576     237      60

Cuts at:   2574    3109    3129 
   Size:        535      20

  Fragments arranged by size: 

                 887     576     535     463     237     174     155      60
                  22      20
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     604     684     856    1039    1057    2722    2815    3129 
   Size:        604      80     172     183      18    1665      93     314

  Fragments arranged by size: 

                1665     604     314     183     172      93      80      18
            
 
BsiEI CG_ry'CG

Cuts at:      0     831    2143    3100    3129 
   Size:        831    1312     957      29

  Fragments arranged by size: 

                1312     957     831      29
 
BsiHKAI G_wGCw'C

Cuts at:      0    1303    1352    1376    3129 
   Size:       1303      49      24    1753

  Fragments arranged by size: 

                1753    1303      49      24
 
BsmI GAATG_Cn'

Cuts at:      0     113    2105    3129 
   Size:        113    1992    1024

  Fragments arranged by size: 

                1992    1024     113
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     450    2874    3129 
   Size:        450    2424     255

  Fragments arranged by size: 

                2424     450     255
 
Bsp1286I G_dGCh'C

Cuts at:      0      14      50     498     512     574     870     995    1079 
   Size:         14      36     448      14      62     296     125      84

Cuts at:   1079    1303    1352    1376    1745    1795    2017    2333    2395 
   Size:        224      49      24     369      50     222     316      62

Cuts at:   2395    2431    2489    2575    2698    3093    3129 
   Size:         36      58      86     123     395      36

  Fragments arranged by size: 

                 448     395     369     316     296     224     222     125
                 123      86      84      62      62      58      50      49
                  36      36      36      24      14      14
 
BspEI T'CCGG_A

Cuts at:      0     803    1910    3129 
   Size:        803    1107    1219

  Fragments arranged by size: 

                1219    1107     803
 
BspHI T'CATG_A

Cuts at:      0    2687    3129 
   Size:       2687     442
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1140    1497    2160    2958    3129 
   Size:       1140     357     663     798     171

  Fragments arranged by size: 

                1140     798     663     357     171
 
BsrBI CCG'CTC

Cuts at:      0     403     863    2445    3129 
   Size:        403     460    1582     684

  Fragments arranged by size: 

                1582     684     460     403
 
BsrDI GCAATG_nn'

Cuts at:      0    1538    3129 
   Size:       1538    1591
 
BsrFI r'CCGG_y

Cuts at:      0    2138    2322    2735    3129 
   Size:       2138     184     413     394

  Fragments arranged by size: 

                2138     413     394     184
 
BssSI C'ACGA_G

Cuts at:      0     670    1456    3129 
   Size:        670     786    1673

  Fragments arranged by size: 

                1673     786     670
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1347    1875    3129 
   Size:       1347     528    1254

  Fragments arranged by size: 

                1347    1254     528
 
BstEII G'GTnAC_C

Cuts at:      0    2823    3129 
   Size:       2823     306
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     505     645     852     948    1326    2094    3129 
   Size:        505     140     207      96     378     768    1035

  Fragments arranged by size: 

                1035     768     505     378     207     140      96
 
BstYI r'GATC_y

Cuts at:      0     302     779     980    1234    1907    3129 
   Size:        302     477     201     254     673    1222

  Fragments arranged by size: 

                1222     673     477     302     254     201
 
BtgI C'CryG_G

Cuts at:      0    1230    1361    1853    2934    3129 
   Size:       1230     131     492    1081     195

  Fragments arranged by size: 

                1230    1081     492     195     131
 
BtsI GCAGTG_nn'

Cuts at:      0     667     918    1034    1104    1662    1888    3129 
   Size:        667     251     116      70     558     226    1241

  Fragments arranged by size: 

                1241     667     558     251     226     116      70
 
ClaI AT'CG_AT

Cuts at:      0     899    3129 
   Size:        899    2230
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     419     454    2722    2757    3129 
   Size:        419      35    2268      35     372

  Fragments arranged by size: 

                2268     419     372      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2823    2910    3129 
   Size:       2823      87     219

  Fragments arranged by size: 

                2823     219      87
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     765    2038    3129 
   Size:        765    1273    1091

  Fragments arranged by size: 

                1273    1091     765
 
EaeI y'GGCC_r

Cuts at:      0     647     701     950    1006    1101    1137    1721    1820 
   Size:        647      54     249      56      95      36     584      99

Cuts at:   1820    2111    2140    2369    2730    3023    3129 
   Size:        291      29     229     361     293     106

  Fragments arranged by size: 

                 647     584     361     293     291     249     229     106
                  99      95      56      54      36      29
 
EagI C'GGCC_G

Cuts at:      0    2140    3129 
   Size:       2140     989
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      52     310    1284    1651    1825    3129 
   Size:         52     258     974     367     174    1304

  Fragments arranged by size: 

                1304     974     367     258     174      52
 
EcoICRI GAG'CTC

Cuts at:      0    1301    3129 
   Size:       1301    1828
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     394     598     899     997    1302    1390    1396 
   Size:         50     344     204     301      98     305      88       6

Cuts at:   1396    1636    1819    1924    1960    1996    2198    2215    2659 
   Size:        240     183     105      36      36     202      17     444

Cuts at:   2659    2695    2929    3129 
   Size:         36     234     200

  Fragments arranged by size: 

                 444     344     305     301     240     234     204     202
                 200     183     105      98      88      50      36      36
                  36      17       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     454    2167    2833    2955    3025    3129 
   Size:        454    1713     666     122      70     104

  Fragments arranged by size: 

                1713     666     454     122     104      70
 
EcoO109I rG'GnC_Cy

Cuts at:      0      47     733    1016    1065    1890    1990    2274    2391 
   Size:         47     686     283      49     825     100     284     117

Cuts at:   2391    2399    2838    2961    3090    3106    3129 
   Size:          8     439     123     129      16      23

  Fragments arranged by size: 

                 825     686     439     284     283     129     123     117
                 100      49      47      23      16       8
 
FseI GG_CCGG'CC

Cuts at:      0    2142    3129 
   Size:       2142     987
 
HaeII r_GCGC'y

Cuts at:      0    1983    3129 
   Size:       1983    1146
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     872     904    1426    1458    2171    2203    2626    2658 
   Size:        872      32     522      32     713      32     423      32

Cuts at:   2658    3129 
   Size:        471

  Fragments arranged by size: 

                 872     713     522     471     423      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    2973    3129 
   Size:       2973     156
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     251    1781    3129 
   Size:        251    1530    1348

  Fragments arranged by size: 

                1530    1348     251
 
MscI TGG'CCA

Cuts at:      0     649     703     952    1139    1822    2113    2732    3129 
   Size:        649      54     249     187     683     291     619     397

  Fragments arranged by size: 

                 683     649     619     397     291     249     187      54
            
 
MslI CAynn'nnrTG

Cuts at:      0     142     503     910    2092    3129 
   Size:        142     361     407    1182    1037

  Fragments arranged by size: 

                1182    1037     407     361     142
 
MspA1I CmG'CkG

Cuts at:      0     618    1005    1053    1061    1394    2145    2518    3129 
   Size:        618     387      48       8     333     751     373     611

  Fragments arranged by size: 

                 751     618     611     387     373     333      48       8
            
 
NaeI GCC'GGC

Cuts at:      0    2140    2324    3129 
   Size:       2140     184     805

  Fragments arranged by size: 

                2140     805     184
 
NcoI C'CATG_G

Cuts at:      0    1853    3129 
   Size:       1853    1276
 
NgoMIV G'CCGG_C

Cuts at:      0    2138    2322    3129 
   Size:       2138     184     807

  Fragments arranged by size: 

                2138     807     184
 
NheI G'CTAG_C

Cuts at:      0    1311    3129 
   Size:       1311    1818
 
NspI r_CATG'y

Cuts at:      0    2616    3129 
   Size:       2616     513
 
PasI CC'CwG_GG

Cuts at:      0     218     774    1995    2395    3129 
   Size:        218     556    1221     400     734

  Fragments arranged by size: 

                1221     734     556     400     218
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1657    2581    3129 
   Size:       1657     924     548

  Fragments arranged by size: 

                1657     924     548
 
PfoI T'CCnGG_A

Cuts at:      0    2882    3129 
   Size:       2882     247
 
PmlI CAC'GTG

Cuts at:      0    1671    2094    3129 
   Size:       1671     423    1035

  Fragments arranged by size: 

                1671    1035     423
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1609    1641    3129 
   Size:       1609      32    1488

  Fragments arranged by size: 

                1609    1488      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     733    1065    1890    1990    2274    2399    2838    2961 
   Size:        733     332     825     100     284     125     439     123

Cuts at:   2961    3129 
   Size:        168

  Fragments arranged by size: 

                 825     733     439     332     284     168     125     123
                 100
 
PspOMI G'GGCC_C

Cuts at:      0      46    2391    3089    3129 
   Size:         46    2345     698      40

  Fragments arranged by size: 

                2345     698      46      40
 
PspXI vC'TCGA_Gb

Cuts at:      0    2716    3129 
   Size:       2716     413
 
PstI C_TGCA'G

Cuts at:      0    1111    1508    2171    2778    2969    3129 
   Size:       1111     397     663     607     191     160

  Fragments arranged by size: 

                1111     663     607     397     191     160
 
PvuI CG_AT'CG

Cuts at:      0    3100    3129 
   Size:       3100      29
 
PvuII CAG'CTG

Cuts at:      0    1053    2518    3129 
   Size:       1053    1465     611

  Fragments arranged by size: 

                1465    1053     611
 
SacI G_AGCT'C

Cuts at:      0    1303    3129 
   Size:       1303    1826
 
SanDI GG'GwC_CC

Cuts at:      0     733    1065    1990    3129 
   Size:        733     332     925    1139

  Fragments arranged by size: 

                1139     925     733     332
 
SbfI CC_TGCA'GG

Cuts at:      0    2171    2969    3129 
   Size:       2171     798     160

  Fragments arranged by size: 

                2171     798     160
 
SexAI A'CCwGG_T

Cuts at:      0    1892    3129 
   Size:       1892    1237
 
SfcI C'TryA_G

Cuts at:      0    1107    1504    2167    2774    2965    3129 
   Size:       1107     397     663     607     191     164

  Fragments arranged by size: 

                1107     663     607     397     191     164
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1014    3129 
   Size:       1014    2115
 
SmaI CCC'GGG

Cuts at:      0    2425    3129 
   Size:       2425     704
 
SmlI C'TyrA_G

Cuts at:      0     346     931    2131    2716    3129 
   Size:        346     585    1200     585     413

  Fragments arranged by size: 

                1200     585     585     413     346
 
StuI AGG'CCT

Cuts at:      0    2051    2300    2451    2727    2942    3129 
   Size:       2051     249     151     276     215     187

  Fragments arranged by size: 

                2051     276     249     215     187     151
 
StyI C'CwwG_G

Cuts at:      0    1853    2740    2807    3129 
   Size:       1853     887      67     322

  Fragments arranged by size: 

                1853     887     322      67
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     278    2493    3129 
   Size:        278    2215     636

  Fragments arranged by size: 

                2215     636     278
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     877     909    1186    1218    3129 
   Size:        877      32     277      32    1911

  Fragments arranged by size: 

                1911     877     277      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1440    1519    2959    3129 
   Size:       1440      79    1440     170

  Fragments arranged by size: 

                1440    1440     170      79
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     945    2064    3129 
   Size:        945    1119    1065

  Fragments arranged by size: 

                1119    1065     945
 
XhoI C'TCGA_G

Cuts at:      0    2716    3129 
   Size:       2716     413
 
XmaI C'CCGG_G

Cuts at:      0    2423    3129 
   Size:       2423     706
 
ZraI GAC'GTC

Cuts at:      0     444    3129 
   Size:        444    2685
 

 Enzymes that do cut:

     AarI    AatII     AclI     AcuI     AfeI     AhdI     AleI     AloI
    AlwNI     ApaI    ApaLI     AvaI    BamHI     BanI    BanII     BbsI
    BbvCI     BclI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI   BstEII    BstXI    BstYI     BtgI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FseI    HaeII    Hin4I   HincII     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NheI     NspI     PasI    PflMI
     PfoI     PmlI     PpiI    PpuMI   PspOMI    PspXI     PstI     PvuI
    PvuII     SacI    SanDI     SbfI    SexAI     SfcI     SfiI     SmaI
     SmlI     StuI     StyI    TaqII     TstI  Tth111I     XcmI     XhoI
     XmaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I    AflII   AflIII     AgeI     AlfI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BbeI     BcgI    BciVI    BfrBI
    BglII    BsaBI    BsiWI    BsrGI   BssHII    BstBI  BstZ17I   Bsu36I
    BtgZI     DraI     EarI    EcoRI    EcoRV     FalI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NarI     NdeI
     NotI     NruI     NsiI     PacI     PciI     PmeI    PshAI     PsiI
     PsrI    RsrII    SacII     SalI     SapI     ScaI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
     XbaI     XmnI
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