Restriction map for pF1KSDA1716
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AviII       
AvrII       BamHI       BbrPI       BbsI        BbvCI       
BclI        BfrI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrGI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MseI        MunI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsaI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tsp509I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccI          769  GTCTAC               GT^[AC][GT]AC
AlwNI        1670  CAGGGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1303  AGCGCT               AGC^GCT
ApaI          338  GGGCCC               GGGCC^C
Asp700I       357  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI           85  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         2093  TGGCCA               TGG^CCA
BciVI        2240  GTATCC               GTATCC(6/5)
BfaI         1909  CTAG                 C^TAG
BglII        1316  AGATCT               A^GATCT
BsaAI        1408  TACGTG               [CT]AC^GT[GA]
BseRI        1702  GAGGAG               GAGGAG(10/8)
BspMI        2234  GCAGGT               ACCTGC(4/8)
BssHII       2146  GCGCGC               G^CGCGC
DraIII        316  CACGAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     1303  AGCGCT               AGC^GCT
EcoNI         126  CCTACAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII       1855  GTCAAC               GT[CT]^[GA]AC
HindII       1855  GTCAAC               GT[CT]^[GA]AC
MaeI         1909  CTAG                 C^TAG
MluNI        2093  TGGCCA               TGG^CCA
MscI         2093  TGGCCA               TGG^CCA
NaeI         1111  GCCGGC               GCC^GGC
NcoI         1439  CCATGG               C^CATGG
NgoMIV       1111  GCCGGC               G^CCGGC
NspI          896  GCATGC               [GA]CATG^[CT]
PflFI          85  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         315  CCACGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI         2221  GAGCTC               GAGCT^C
SapI         1641  GCTCTTC              GCTCTTC(1/4)
SphI          896  GCATGC               GCATG^C
TfiI         1311  GAATC                G^A[AT]TC
Tth111I        85  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        315  CCACGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1160  CCAGCATCAACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          357  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BcgI         1812  CGACCTTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
               35  GCAGGCTTTTCG        
BstYI         428  AGATCC               [GA]^GATC[CT]
             1316  AGATCT              
EcoT14I      1232  CCAAGG               C^C[AT][AT]GG
             1439  CCATGG              
MaeIII        879  GTCAC                ^GT[ACGT]AC
             2128  GTGAC               
MflI          428  AGATCC               [GA]^GATC[CT]
             1316  AGATCT              
MslI          798  CACCGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2097  CATCGCAGTG          
PpuMI         263  AGGACCT              [GA]G^G[AT]CC[CT]
             2084  GGGACCC             
SfiI         1839  GGCCCACGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2014  GGCCGCACCGGCC       
SmaI         1776  CCCGGG               CCC^GGG
             2193  CCCGGG              
SmlI          784  CTCAAG               C^T[CT][GA]AG
             1540  CTTGAG              
StyI         1232  CCAAGG               C^C[AT][AT]GG
             1439  CCATGG              
Tsp45I        879  GTCAC                ^GT[GC]AC
             2128  GTGAC               
XhoII         428  AGATCC               [GA]^GATC[CT]
             1316  AGATCT              
XmaCI        1776  CCCGGG               C^CCGGG
             2193  CCCGGG              
XmaI         1776  CCCGGG               C^CCGGG
             2193  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
AspHI       AvaI        AvaII       BanI        BanII       
BbeI        BbvI        BcnI        BglI        BmyI        
BpmI        BsaHI       BsaJI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrBI       BsrFI       BsrI        BssKI       
BssSI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeII       
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NdeII       NlaIII      NlaIV       PleI        PstI        
PvuII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        StuI        TaqI        TseI        
TspRI       TthHB8I     
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