Restriction map for pF1KSDA1716
 (Linear) (Six-base) MAPSORT of: KIAA1716.seq  Check: 885  from: 1  to: 2277



KIAA1716.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2225    2277 
   Size:       2225      52
 
AccI GT'mk_AC

Cuts at:      0     770    2277 
   Size:        770    1507
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2062    2277 
   Size:       2062     215
 
AfeI AGC'GCT

Cuts at:      0    1305    2277 
   Size:       1305     972
 
AleI CACnn'nnGTG

Cuts at:      0     802    2277 
   Size:        802    1475
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1582    1614    2212    2244    2277 
   Size:       1582      32     598      32      33

  Fragments arranged by size: 

                1582     598      33      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1675    2277 
   Size:       1675     602
 
ApaI G_GGCC'C

Cuts at:      0     342    2277 
   Size:        342    1935
 
AvaI C'yCGr_G

Cuts at:      0     300    1058    1776    2193    2277 
   Size:        300     758     718     417      84

  Fragments arranged by size: 

                 758     718     417     300      84
 
BanI G'GyrC_C

Cuts at:      0     305    1434    1443    1582    1986    2197    2277 
   Size:        305    1129       9     139     404     211      80

  Fragments arranged by size: 

                1129     404     305     211     139      80       9
 
BanII G_rGCy'C

Cuts at:      0     342     982    1547    1784    1918    2225    2277 
   Size:        342     640     565     237     134     307      52

  Fragments arranged by size: 

                 640     565     342     307     237     134      52
 
BbeI G_GCGC'C

Cuts at:      0    1438    1990    2201    2277 
   Size:       1438     552     211      76

  Fragments arranged by size: 

                1438     552     211      76
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      24      58    1801    1835    2277 
   Size:         24      34    1743      34     442

  Fragments arranged by size: 

                1743     442      34      34      24
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2251    2277 
   Size:       2251      26
 
BglI GCCn_nnn'nGGC

Cuts at:      0      16     337    1127    1456    1672    1846    2021    2277 
   Size:         16     321     790     329     216     174     175     256

  Fragments arranged by size: 

                 790     329     321     256     216     175     174      16
            
 
BglII A'GATC_T

Cuts at:      0    1316    2277 
   Size:       1316     961
 
Bme1580I G_kGCm'C

Cuts at:      0     342    1585    1809    2277 
   Size:        342    1243     224     468

  Fragments arranged by size: 

                1243     468     342     224
 
BmgBI CAC'GTC

Cuts at:      0     660    1761    1959    2277 
   Size:        660    1101     198     318

  Fragments arranged by size: 

                1101     660     318     198
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     456     665    1323    1869    2277 
   Size:        456     209     658     546     408

  Fragments arranged by size: 

                 658     546     456     408     209
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1630    1653    2277 
   Size:       1630      23     624

  Fragments arranged by size: 

                1630     624      23
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     289     376    1474    1491    2209    2277 
   Size:        178     111      87    1098      17     718      68

  Fragments arranged by size: 

                1098     718     178     111      87      68      17
 
Bpu10I CC'TnA_GC

Cuts at:      0    1268    2277 
   Size:       1268    1009
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     769    1561    2277 
   Size:        769     792     716

  Fragments arranged by size: 

                 792     769     716
 
BsaAI yAC'GTr

Cuts at:      0    1410    2277 
   Size:       1410     867
 
BsaHI Gr'CG_yC

Cuts at:      0      53    1435    1888    1987    2198    2277 
   Size:         53    1382     453      99     211      79

  Fragments arranged by size: 

                1382     453     211      99      79      53
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1203    1233    1943    1973    2212    2242    2277 
   Size:       1203      30     710      30     239      30      35

  Fragments arranged by size: 

                1203     710     239      35      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1717    2277 
   Size:       1717     560
 
BseYI C'CCAG_C

Cuts at:      0      68    1154    2010    2277 
   Size:         68    1086     856     267

  Fragments arranged by size: 

                1086     856     267      68
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     481     967    1120    1361    1498    1712    1756    1918 
   Size:        481     486     153     241     137     214      44     162

Cuts at:   1918    1978    2125    2277 
   Size:         60     147     152

  Fragments arranged by size: 

                 486     481     241     214     162     153     152     147
                 137      60      44
 
BsiHKAI G_wGCw'C

Cuts at:      0     392     829    1198    2225    2277 
   Size:        392     437     369    1027      52

  Fragments arranged by size: 

                1027     437     392     369      52
 
Bsp1286I G_dGCh'C

Cuts at:      0     342     392     829     982    1198    1547    1585    1784 
   Size:        342      50     437     153     216     349      38     199

Cuts at:   1784    1809    1918    2225    2277 
   Size:         25     109     307      52

  Fragments arranged by size: 

                 437     349     342     307     216     199     153     109
                  52      50      38      25
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2225    2277 
   Size:       2225      52
 
BsrBI CCG'CTC

Cuts at:      0      50     855    1567    1954    2277 
   Size:         50     805     712     387     323

  Fragments arranged by size: 

                 805     712     387     323      50
 
BsrFI r'CCGG_y

Cuts at:      0      10     308     734    1111    2020    2277 
   Size:         10     298     426     377     909     257

  Fragments arranged by size: 

                 909     426     377     298     257      10
 
BssHII G'CGCG_C

Cuts at:      0    2146    2277 
   Size:       2146     131
 
BssSI C'ACGA_G

Cuts at:      0     316    1029    1257    2277 
   Size:        316     713     228    1020

  Fragments arranged by size: 

                1020     713     316     228
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1098    1193    1804    1905    2277 
   Size:       1098      95     611     101     372

  Fragments arranged by size: 

                1098     611     372     101      95
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     678    1469    2010    2277 
   Size:        678     791     541     267

  Fragments arranged by size: 

                 791     678     541     267
 
BstYI r'GATC_y

Cuts at:      0     428    1316    2277 
   Size:        428     888     961

  Fragments arranged by size: 

                 961     888     428
 
BtgI C'CryG_G

Cuts at:      0     800    1439    1842    2216    2277 
   Size:        800     639     403     374      61

  Fragments arranged by size: 

                 800     639     403     374      61
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     942    2082    2277 
   Size:        942    1140     195

  Fragments arranged by size: 

                1140     942     195
 
BtsI GCAGTG_nn'

Cuts at:      0      79    1224    1517    2108    2131    2277 
   Size:         79    1145     293     591      23     146

  Fragments arranged by size: 

                1145     591     293     146      79      23
 
DraIII CAC_nnn'GTG

Cuts at:      0     321    2277 
   Size:        321    1956
 
EaeI y'GGCC_r

Cuts at:      0    1109    1137    1562    2022    2093    2111    2277 
   Size:       1109      28     425     460      71      18     166

  Fragments arranged by size: 

                1109     460     425     166      71      28      18
 
EarI CTCTTCn'nnn_

Cuts at:      0     703    1621    1648    2264    2277 
   Size:        703     918      27     616      13

  Fragments arranged by size: 

                 918     703     616      27      13
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     355     808     954    1035    1591    1771    1879    2170 
   Size:        355     453     146      81     556     180     108     291

Cuts at:   2170    2277 
   Size:        107

  Fragments arranged by size: 

                 556     453     355     291     180     146     108     107
                  81
 
EcoICRI GAG'CTC

Cuts at:      0    2223    2277 
   Size:       2223      54
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     289     376    1474    1491    2062    2209    2277 
   Size:        178     111      87    1098      17     571     147      68

  Fragments arranged by size: 

                1098     571     178     147     111      87      68      17
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     130    2277 
   Size:        130    2147
 
EcoO109I rG'GnC_Cy

Cuts at:      0     264     297     338     339    1458    1749    2085    2277 
   Size:        264      33      41       1    1119     291     336     192

  Fragments arranged by size: 

                1119     336     291     264     192      41      33       1
            
 
HaeII r_GCGC'y

Cuts at:      0     741    1307    1438    1837    1990    2201    2277 
   Size:        741     566     131     399     153     211      76

  Fragments arranged by size: 

                 741     566     399     211     153     131      76
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     862     894    2277 
   Size:        862      32    1383

  Fragments arranged by size: 

                1383     862      32
 
HincII GTy'rAC

Cuts at:      0    1857    2277 
   Size:       1857     420
 
KasI G'GCGC_C

Cuts at:      0    1434    1986    2197    2277 
   Size:       1434     552     211      80

  Fragments arranged by size: 

                1434     552     211      80
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1679    2277 
   Size:       1679     598
 
MscI TGG'CCA

Cuts at:      0    2095    2277 
   Size:       2095     182
 
MslI CAynn'nnrTG

Cuts at:      0     802    2101    2277 
   Size:        802    1299     176

  Fragments arranged by size: 

                1299     802     176
 
MspA1I CmG'CkG

Cuts at:      0     504     546     609     765    1154    1712    1893    2082 
   Size:        504      42      63     156     389     558     181     189

Cuts at:   2082    2277 
   Size:        195

  Fragments arranged by size: 

                 558     504     389     195     189     181     156      63
                  42
 
NaeI GCC'GGC

Cuts at:      0    1113    2277 
   Size:       1113    1164
 
NarI GG'CG_CC

Cuts at:      0    1435    1987    2198    2277 
   Size:       1435     552     211      79

  Fragments arranged by size: 

                1435     552     211      79
 
NcoI C'CATG_G

Cuts at:      0    1439    2277 
   Size:       1439     838
 
NgoMIV G'CCGG_C

Cuts at:      0    1111    2277 
   Size:       1111    1166
 
NspI r_CATG'y

Cuts at:      0     900    2277 
   Size:        900    1377
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     321    2277 
   Size:        321    1956
 
PfoI T'CCnGG_A

Cuts at:      0     431    2243    2277 
   Size:        431    1812      34

  Fragments arranged by size: 

                1812     431      34
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     844     876    2212    2244    2277 
   Size:        844      32    1336      32      33

  Fragments arranged by size: 

                1336     844      33      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     264    2085    2277 
   Size:        264    1821     192

  Fragments arranged by size: 

                1821     264     192
 
PspOMI G'GGCC_C

Cuts at:      0     338    2277 
   Size:        338    1939
 
PstI C_TGCA'G

Cuts at:      0     110     410     623     905    1094    2277 
   Size:        110     300     213     282     189    1183

  Fragments arranged by size: 

                1183     300     282     213     189     110
 
PvuII CAG'CTG

Cuts at:      0     504     546     765    2277 
   Size:        504      42     219    1512

  Fragments arranged by size: 

                1512     504     219      42
 
SacI G_AGCT'C

Cuts at:      0    2225    2277 
   Size:       2225      52
 
SanDI GG'GwC_CC

Cuts at:      0    2085    2277 
   Size:       2085     192
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1648    2277 
   Size:       1648     629
 
SfcI C'TryA_G

Cuts at:      0     106     127     406     486     619     901     993    1090 
   Size:        106      21     279      80     133     282      92      97

Cuts at:   1090    2277 
   Size:       1187

  Fragments arranged by size: 

                1187     282     279     133     106      97      92      80
                  21
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1846    2021    2277 
   Size:       1846     175     256

  Fragments arranged by size: 

                1846     256     175
 
SfoI GGC'GCC

Cuts at:      0    1436    1988    2199    2277 
   Size:       1436     552     211      78

  Fragments arranged by size: 

                1436     552     211      78
 
SmaI CCC'GGG

Cuts at:      0    1778    2195    2277 
   Size:       1778     417      82

  Fragments arranged by size: 

                1778     417      82
 
SmlI C'TyrA_G

Cuts at:      0     784    1540    2277 
   Size:        784     756     737

  Fragments arranged by size: 

                 784     756     737
 
SphI G_CATG'C

Cuts at:      0     900    2277 
   Size:        900    1377
 
StuI AGG'CCT

Cuts at:      0      19    1390    1744    2277 
   Size:         19    1371     354     533

  Fragments arranged by size: 

                1371     533     354      19
 
StyI C'CwwG_G

Cuts at:      0    1232    1439    2277 
   Size:       1232     207     838

  Fragments arranged by size: 

                1232     838     207
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     897    1207    2277 
   Size:        897     310    1070

  Fragments arranged by size: 

                1070     897     310
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     448     480    1203    1235    1943    1975    2277 
   Size:        448      32     723      32     708      32     302

  Fragments arranged by size: 

                 723     708     448     302      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      88    2277 
   Size:         88    2189
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1167    2277 
   Size:       1167    1110
 
XmaI C'CCGG_G

Cuts at:      0    1776    2193    2277 
   Size:       1776     417      84

  Fragments arranged by size: 

                1776     417      84
 
XmnI GAAnn'nnTTC

Cuts at:      0     361    2277 
   Size:        361    1916
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI     AleI     AloI    AlwNI     ApaI
     AvaI     BanI    BanII     BbeI     BcgI    BciVI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI
    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstXI    BstYI     BtgI
    BtgZI     BtsI   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    HaeII    Hin4I   HincII     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI    PflMI
     PfoI     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI    SanDI
     SapI     SfcI     SfiI     SfoI     SmaI     SmlI     SphI     StuI
     StyI    TaqII     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AlfI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbsI    BbvCI     BclI    BfrBI     BlpI     BmtI     BsaI    BsaBI
    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI
    BsrGI    BstBI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI
     DrdI     EagI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
  HindIII     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SbfI
     ScaI    SexAI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI
     SwaI     TatI     XbaI     XhoI     ZraI
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