################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## Acc65I AccII AccIII AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AviII BamHI BbeI BbrPI BcgI BcnI BglII BlpI Bpu1102I BsaI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspMI BsrBI BsrFI BssHII BstBI BstEII BstPI BstUI CelII Cfr10I ClaI CpoI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I FseI FspI HaeII HapII HindIII HpaII KasI KpnI KspI MluI MroI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AatII 1786 GACGTC GACGT^C AccI 829 GTATAC GT^[AC][GT]AC AclI 2960 AACGTT AA^CGTT AflII 2949 CTTAAG C^TTAAG Alw44I 2844 GTGCAC G^TGCAC AocI 2904 CCTCAGG CC^T[ACGT]AGG ApaLI 2844 GTGCAC G^TGCAC AspI 1831 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 353 CTCGGG C^[CT]CG[GA]G AvrII 1186 CCTAGG C^CTAGG BalI 2 TGGCCA TGG^CCA BanII 1358 GAGCTC G[GA]GC[CT]^C BfrI 2949 CTTAAG C^TTAAG BglI 1736 GCCTGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1186 CCTAGG C^CTAGG BpmI 2149 CTCCAG CTGGAG(16/14) BsaAI 941 CACGTA [CT]AC^GT[GA] BsaBI 2455 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 1500 GTGCAG GTGCAG(16/14) BsoBI 353 CTCGGG C^[CT]CG[GA]G Bsp1407I 597 TGTACA T^GTACA BspHI 2675 TCATGA T^CATGA BsrDI 384 CATTGC GCAATG(2/0) BsrGI 597 TGTACA T^GTACA BssSI 1670 CTCGTG CACGAG(-5/-1) Bst1107I 829 GTATAC GTA^TAC BstAPI 1502 GCAGCTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 639 CCAAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 829 GTATAC GTA^TAC Bsu36I 2904 CCTCAGG CC^T[ACGT]AGG CfoI 381 GCGC GCG^C DrdI 1780 GACCTTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 2 TGGCCA [CT]^GGCC[GA] Eco81I 2904 CCTCAGG CC^T[ACGT]AGG EcoRI 1453 GAATTC G^AATTC EcoRV 1963 GATATC GAT^ATC FauI 2871 GCGGG CCCGC(4/6) HgaI 1276 GACGC GACGC(5/10) HhaI 381 GCGC GCG^C HinP1I 381 GCGC G^CGC MaeIII 1778 GTGAC ^GT[ACGT]AC MamI 2455 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1922 CAATTG C^AATTG MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MunI 1922 CAATTG C^AATTG PflFI 1831 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 212 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2960 AACGTT AA^CGTT PstI 152 CTGCAG CTGCA^G RcaI 2675 TCATGA T^CATGA SacI 1358 GAGCTC GAGCT^C SfcI 152 CTGCAG C^T[GA][CT]AG SspBI 597 TGTACA T^GTACA TaqI 2649 TCGA T^CGA Tsp45I 1778 GTGAC ^GT[GC]AC Tth111I 1831 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 2649 TCGA T^CGA XbaI 2098 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 1276 GACGCC G[GA]^CG[CT]C 1786 GACGTC AlwNI 2300 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG 2876 CAGCTGCTG AseI 222 ATTAAT AT^TAAT 1801 ATTAAT AsnI 222 ATTAAT AT^TAAT 1801 ATTAAT BanI 183 GGCACC G^G[CT][GA]CC 1917 GGTGCC BbvCI 3065 CCTCAGC CCTCAGC(-5/-2) 1819 GCTGAGG BciVI 785 GTATCC GTATCC(6/5) 1622 GTATCC BclI 903 TGATCA T^GATCA 2250 TGATCA BsaHI 1276 GACGCC G[GA]^CG[CT]C 1786 GACGTC BsmI 540 GAATGC GAATGC(1/-1) 3001 GAATGC DraII 642 AGGTCCT [GA]G^G[ACGT]CC[CT] 3076 AGGACCC EcoO109I 642 AGGTCCT [GA]G^G[ACGT]CC[CT] 3076 AGGACCC EcoT14I 639 CCAAGG C^C[AT][AT]GG 1186 CCTAGG EcoT22I 2093 ATGCAT ATGCA^T 2120 ATGCAT FbaI 903 TGATCA T^GATCA 2250 TGATCA Hin1I 1276 GACGCC G[GA]^CG[CT]C 1786 GACGTC HpaI 799 GTTAAC GTT^AAC 2610 GTTAAC MslI 589 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 754 CACATCAGTG MspA1I 662 CAGCTG C[AC]G^C[GT]G 2876 CAGCTG NsiI 2093 ATGCAT ATGCA^T 2120 ATGCAT PpuMI 642 AGGTCCT [GA]G^G[AT]CC[CT] 3076 AGGACCC PshBI 222 ATTAAT AT^TAAT 1801 ATTAAT PvuII 662 CAGCTG CAG^CTG 2876 CAGCTG SapI 496 GCTCTTC GCTCTTC(1/4) 3020 GCTCTTC SmlI 1390 CTCAAG C^T[CT][GA]AG 2949 CTTAAG StyI 639 CCAAGG C^C[AT][AT]GG 1186 CCTAGG XcmI 1938 CCATTTGAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2914 CCAGTAGAAAAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI Asp700I AspHI AvaII BbsI BbvI BfaI BmyI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SspI TfiI Tru9I TseI Tsp509I TspRI XhoII XmnI