Restriction map for pF1KSDA1704
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BlnI        BsaAI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        Bsp1407I    
BspEI       BspHI       BspLU11I    BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      CfoI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HphI        KasI        KpnI        MaeII       
MfeI        MluI        MluNI       MroI        MscI        
MslI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PstI        PvuI        
RcaI        SacI        SalI        SbfI        ScaI        
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     Van91I      XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccII          49  CGCG                 CG^CG
AfaI          168  GTAC                 GT^AC
AlwI          394  GATCC                GGATC(4/5)
ApaI          500  GGGCCC               GGGCC^C
AvaI           78  CCCGAG               C^[CT]CG[GA]G
BbvI          862  GCTGC                GCAGC(8/12)
BcnI           34  CCCGG                CC^[GC]GG
BfaI          971  CTAG                 C^TAG
BglII         915  AGATCT               A^GATCT
BlpI          781  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      781  GCTGAGC              GC^T[ACGT]AGC
BsaI            8  GAGACC               GGTCTC(1/5)
BseRI         314  CTCCTC               GAGGAG(10/8)
BsmFI          74  GGGAC                GGGAC(10/14)
BsoBI          78  CCCGAG               C^[CT]CG[GA]G
BspDI         656  ATCGAT               AT^CGAT
BspMI         786  GCAGGT               ACCTGC(4/8)
BsrBI          70  GAGCGG               CCGCTC(-3/-3)
BsrDI         341  CATTGC               GCAATG(2/0)
BsrFI          21  ACCGGC               [GA]^CCGG[CT]
BstUI          49  CGCG                 CG^CG
BstYI         915  AGATCT               [GA]^GATC[CT]
CelII         781  GCTGAGC              GC^T[ACGT]AGC
Cfr10I         21  ACCGGC               [GA]^CCGG[CT]
ClaI          656  ATCGAT               AT^CGAT
CpoI           18  CGGACCG              CG^G[AT]CCG
DraI          295  TTTAAA               TTT^AAA
DsaI           48  CCGCGG               C^C[GA][CT]GG
HincII        466  GTTAAC               GT[CT]^[GA]AC
HindII        466  GTTAAC               GT[CT]^[GA]AC
HindIII       880  AAGCTT               A^AGCTT
HpaI          466  GTTAAC               GTT^AAC
KspI           48  CCGCGG               CCGC^GG
MaeI          971  CTAG                 C^TAG
MflI          915  AGATCT               [GA]^GATC[CT]
MvnI           49  CGCG                 CG^CG
MwoI           91  GCAGGACCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI           34  CCCGG                CC^[GC]GG
NlaIII        569  CATG                 CATG^
PleI          565  GAGTC                GAGTC(4/5)
PvuII         680  CAGCTG               CAG^CTG
RsaI          168  GTAC                 GT^AC
RsrII          18  CGGACCG              CG^G[AT]CCG
SacII          48  CCGCGG               CCGC^GG
SexAI         348  ACCTGGT              A^CC[AT]GGT
SmlI          919  CTCAAG               C^T[CT][GA]AG
TaqI          657  TCGA                 T^CGA
TseI          862  GCTGC                G^C[AT]GC
Tsp45I        377  GTGAC                ^GT[GC]AC
Tsp509I       112  AATT                 ^AATT
TthHB8I       657  TCGA                 T^CGA
XbaI          970  TCTAGA               T^CTAGA
XhoII         915  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

BanII          81  GAGCCC               G[GA]GC[CT]^C
              500  GGGCCC              
BbsI          151  GAAGAC               GAAGAC(2/6)
              868  GAAGAC              
BmyI           81  GAGCCC               G[AGT]GC[ACT]^C
              500  GGGCCC              
BpuAI         151  GAAGAC               GAAGAC(2/6)
              868  GAAGAC              
BsaBI         815  GATCAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              910  GATAAAGATC          
BsmAI           8  GAGAC                GTCTC(1/5)
              774  GAGAC               
Bsp1286I       81  GAGCCC               G[AGT]GC[ACT]^C
              500  GGGCCC              
Cac8I          46  GCCCGC               GC[ACGT]^[ACGT]GC
              450  GCCTGC              
EcoNI          85  CCTGTTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              451  CCTGCAAAAGG         
EcoT14I       319  CCAAGG               C^C[AT][AT]GG
              616  CCAAGG              
FauI           47  CCCGC                CCCGC(4/6)
               72  GCGGG               
Fnu4HI         30  GCCGC                GC^[ACGT]GC
              862  GCTGC               
HapII          22  CCGG                 C^CGG
               35  CCGG                
HpaII          22  CCGG                 C^CGG
               35  CCGG                
ItaI           30  GCCGC                GC^[ACGT]GC
              862  GCTGC               
MaeIII        377  GTGAC                ^GT[ACGT]AC
              478  GTAAC               
MamI          815  GATCAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              910  GATAAAGATC          
MspI           22  CCGG                 C^CGG
               35  CCGG                
PpuMI          74  GGGACCC              [GA]G^G[AT]CC[CT]
              333  AGGTCCT             
PshAI         202  GACAGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
              601  GACTTTGGTC          
SapI           67  GAAGAGC              GCTCTTC(1/4)
              632  GAAGAGC             
StyI          319  CCAAGG               C^C[AT][AT]GG
              616  CCAAGG              
TspRI         204  CAGTG                CA[GC]TG[ACGT][ACGT]^
              420  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AciI        AluI        AvaII       BpmI        BsaJI       
BsiYI       BslI        BsrI        BssKI       BstF5I      
BstNI       Cfr13I      DdeI        DpnII       DraII       
EarI        EcoO109I    EcoRII      FokI        HaeIII      
HinfI       Ksp632I     MboI        MboII       MnlI        
MseI        MspA1I      MvaI        NdeII       NlaIV       
Sau3AI      Sau96I      ScrFI       TfiI        Tru9I       
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