Restriction map for pF1KSDA1605
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       AclI        AcsI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AviII       BbeI        
BbrPI       BciVI       BfrI        BglII       BsaAI       
BsaBI       BsaI        BsiWI       BsmI        BspDI       
BsrBI       BssHII      BstBI       Bsu36I      ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoRI       
EcoRV       FseI        FspI        HincII      HindII      
HindIII     HpaI        KasI        KspI        MamI        
MfeI        MluI        MunI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
Tth111I     XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccII        1813  CGCG                 CG^CG
AccIII        183  TCCGGA               T^CCGGA
AcyI           34  GGCGTC               G[GA]^CG[CT]C
AlwNI        1319  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvrII        2292  CCTAGG               C^CTAGG
BglI         2389  GCCATGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         2292  CCTAGG               C^CTAGG
BlpI         1665  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1665  GCTCAGC              GC^T[ACGT]AGC
BsaHI          34  GGCGTC               G[GA]^CG[CT]C
BseAI         183  TCCGGA               T^CCGGA
BsiEI        1560  CGGTCG               CG[GA][CT]^CG
Bsp1407I     1598  TGTACA               T^GTACA
BspEI         183  TCCGGA               T^CCGGA
BspHI         273  TCATGA               T^CATGA
BsrDI        1161  CATTGC               GCAATG(2/0)
BsrFI          31  ACCGGC               [GA]^CCGG[CT]
BsrGI        1598  TGTACA               T^GTACA
BssSI        1016  CACGAG               CACGAG(-5/-1)
Bst1107I     1287  GTATAC               GTA^TAC
BstAPI       1318  GCAGCACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1813  CGCG                 CG^CG
BstZ17I      1287  GTATAC               GTA^TAC
CelII        1665  GCTCAGC              GC^T[ACGT]AGC
Cfr10I         31  ACCGGC               [GA]^CCGG[CT]
EcoNI        1503  CCTACCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      1820  ATGCAT               ATGCA^T
FauI          944  GCGGG                CCCGC(4/6)
HaeII         626  AGCGCC               [GA]GCGC^[CT]
Hin1I          34  GGCGTC               G[GA]^CG[CT]C
MroI          183  TCCGGA               T^CCGGA
MvnI         1813  CGCG                 CG^CG
NdeI         1607  CATATG               CA^TATG
NsiI         1820  ATGCAT               ATGCA^T
PaeR7I       1199  CTCGAG               C^TCGAG
PshAI        1555  GACTACGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          273  TCATGA               T^CATGA
SapI         2611  GCTCTTC              GCTCTTC(1/4)
SexAI         236  ACCAGGT              A^CC[AT]GGT
SspBI        1598  TGTACA               T^GTACA
TfiI         1957  GAATC                G^A[AT]TC
XbaI          846  TCTAGA               T^CTAGA
XhoI         1199  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccI         1287  GTATAC               GT^[AC][GT]AC
             2473  GTCTAC              
AvaI          698  CCCGAG               C^[CT]CG[GA]G
             1199  CTCGAG              
BamHI          12  GGATCC               G^GATCC
               72  GGATCC              
BbvCI        1329  CCTCAGC              CCTCAGC(-5/-2)
             2154  CCTCAGC             
BcgI         1201  CGAGGCCAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1878  GCAGGTTTATCG        
BclI          419  TGATCA               T^GATCA
             1908  TGATCA              
BseRI        2544  CTCCTC               GAGGAG(10/8)
             1510  GAGGAG              
BsoBI         698  CCCGAG               C^[CT]CG[GA]G
             1199  CTCGAG              
BstEII       1038  GGTAACC              G^GT[ACGT]ACC
             2037  GGTGACC             
BstPI        1038  GGTAACC              G^GT[ACGT]ACC
             2037  GGTGACC             
BstXI        1776  CCATGATATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2256  CCAGTGTGCTGG        
DraIII        570  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1473  CACAGTGTG           
EcoO65I      1038  GGTAACC              G^GT[ACGT]ACC
             2037  GGTGACC             
FbaI          419  TGATCA               T^GATCA
             1908  TGATCA              
MseI          524  TTAA                 T^TAA
             1828  TTAA                
PflMI        2325  CCAACATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2549  CCACTTGCTGG         
PstI          364  CTGCAG               CTGCA^G
             1876  CTGCAG              
StuI         2282  AGGCCT               AGG^CCT
             2372  AGGCCT              
Tru9I         524  TTAA                 T^TAA
             1828  TTAA                
Tsp509I       424  AATT                 ^AATT
              566  AATT                
Van91I       2325  CCAACATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2549  CCACTTGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

Acc65I      AciI        AfaI        AflIII      AluI        
AlwI        Asp718I     AspHI       AvaII       BalI        
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaJI       
BsaWI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       Bsp1286I    BspLU11I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        KpnI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PpuMI       PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     XcmI        XhoII       
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