Restriction map for pF1KSDA1605
 (Linear) (Six-base) MAPSORT of: KIAA1605.seq  Check: 2858  from: 1  to: 2631



KIAA1605.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     754    1071    1332    2631 
   Size:        754     317     261    1299

  Fragments arranged by size: 

                1299     754     317     261
 
AccI GT'mk_AC

Cuts at:      0    1288    2474    2631 
   Size:       1288    1186     157

  Fragments arranged by size: 

                1288    1186     157
 
Acc65I G'GTAC_C

Cuts at:      0     359     374    1724    2631 
   Size:        359      15    1350     907

  Fragments arranged by size: 

                1350     907     359      15
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113    1084    1885    1942    2299    2498    2599    2631 
   Size:        113     971     801      57     357     199     101      32

  Fragments arranged by size: 

                 971     801     357     199     113     101      57      32
            
 
AflIII A'CryG_T

Cuts at:      0    1526    1928    2328    2631 
   Size:       1526     402     400     303

  Fragments arranged by size: 

                1526     402     400     303
 
AleI CACnn'nnGTG

Cuts at:      0    2311    2631 
   Size:       2311     320
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1439    1471    1793    1825    2631 
   Size:       1439      32     322      32     806

  Fragments arranged by size: 

                1439     806     322      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1324    2631 
   Size:       1324    1307
 
AvaI C'yCGr_G

Cuts at:      0     698    1199    2631 
   Size:        698     501    1432

  Fragments arranged by size: 

                1432     698     501
 
AvrII C'CTAG_G

Cuts at:      0    2292    2631 
   Size:       2292     339
 
BamHI G'GATC_C

Cuts at:      0      12      72    2631 
   Size:         12      60    2559

  Fragments arranged by size: 

                2559      60      12
 
BanI G'GyrC_C

Cuts at:      0     106     240     359     374    1724    1746    2622    2631 
   Size:        106     134     119      15    1350      22     876       9

  Fragments arranged by size: 

                1350     876     134     119     106      22      15       9
            
 
BanII G_rGCy'C

Cuts at:      0     926    1393    1667    2507    2564    2631 
   Size:        926     467     274     840      57      67

  Fragments arranged by size: 

                 926     840     467     274      67      57
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     152     388     406    2631 
   Size:        152     236      18    2225

  Fragments arranged by size: 

                2225     236     152      18
 
BbvCI CC'TCA_GC

Cuts at:      0    1330    2155    2631 
   Size:       1330     825     476

  Fragments arranged by size: 

                1330     825     476
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1190    1224    1867    1901    2631 
   Size:       1190      34     643      34     730

  Fragments arranged by size: 

                1190     730     643      34      34
 
BclI T'GATC_A

Cuts at:      0     419    1908    2631 
   Size:        419    1489     723

  Fragments arranged by size: 

                1489     723     419
 
BfrBI ATG'CAT

Cuts at:      0    1822    2631 
   Size:       1822     809
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2395    2631 
   Size:       2395     236
 
BlpI GC'TnA_GC

Cuts at:      0    1666    2631 
   Size:       1666     965
 
Bme1580I G_kGCm'C

Cuts at:      0    2124    2631 
   Size:       2124     507
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     663     892    2045    2631 
   Size:        663     229    1153     586

  Fragments arranged by size: 

                1153     663     586     229
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1392    2631 
   Size:       1392    1239
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     516     643     955    1187    1237    2631 
   Size:        516     127     312     232      50    1394

  Fragments arranged by size: 

                1394     516     312     232     127      50
 
Bpu10I CC'TnA_GC

Cuts at:      0     322    1330    2155    2631 
   Size:        322    1008     825     476

  Fragments arranged by size: 

                1008     825     476     322
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     375    1513    1597    1681    2631 
   Size:        375    1138      84      84     950

  Fragments arranged by size: 

                1138     950     375      84      84
 
BsaHI Gr'CG_yC

Cuts at:      0      35    2631 
   Size:         35    2596
 
BsaWI w'CCGG_w

Cuts at:      0     183     377     545    2631 
   Size:        183     194     168    2086

  Fragments arranged by size: 

                2086     194     183     168
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     301     331     797     827    1460    1490    1793    1823 
   Size:        301      30     466      30     633      30     303      30

Cuts at:   1823    2631 
   Size:        808

  Fragments arranged by size: 

                 808     633     466     303     301      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1525    2535    2631 
   Size:       1525    1010      96

  Fragments arranged by size: 

                1525    1010      96
 
BseYI C'CCAG_C

Cuts at:      0      39    1515    2555    2631 
   Size:         39    1476    1040      76

  Fragments arranged by size: 

                1476    1040      76      39
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     998    2464    2631 
   Size:        139     859    1466     167

  Fragments arranged by size: 

                1466     859     167     139
 
BsiEI CG_ry'CG

Cuts at:      0    1563    2631 
   Size:       1563    1068
 
BsiHKAI G_wGCw'C

Cuts at:      0    1667    2343    2507    2564    2631 
   Size:       1667     676     164      57      67

  Fragments arranged by size: 

                1667     676     164      67      57
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     160     321     887    2631 
   Size:        160     161     566    1744

  Fragments arranged by size: 

                1744     566     161     160
 
Bsp1286I G_dGCh'C

Cuts at:      0     926    1393    1667    2124    2343    2507    2564    2631 
   Size:        926     467     274     457     219     164      57      67

  Fragments arranged by size: 

                 926     467     457     274     219     164      67      57
            
 
BspEI T'CCGG_A

Cuts at:      0     183    2631 
   Size:        183    2448
 
BspHI T'CATG_A

Cuts at:      0     273    2631 
   Size:        273    2358
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     111     371     754    1071    1332    1869    2631 
   Size:        111     260     383     317     261     537     762

  Fragments arranged by size: 

                 762     537     383     317     261     260     111
 
BsrDI GCAATG_nn'

Cuts at:      0    1160    2631 
   Size:       1160    1471
 
BsrFI r'CCGG_y

Cuts at:      0      31    2631 
   Size:         31    2600
 
BsrGI T'GTAC_A

Cuts at:      0    1598    2631 
   Size:       1598    1033
 
BssSI C'ACGA_G

Cuts at:      0    1016    2631 
   Size:       1016    1615
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1324    2631 
   Size:       1324    1307
 
BstEII G'GTnAC_C

Cuts at:      0    1038    2037    2631 
   Size:       1038     999     594

  Fragments arranged by size: 

                1038     999     594
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1783    2263    2631 
   Size:       1783     480     368

  Fragments arranged by size: 

                1783     480     368
 
BstYI r'GATC_y

Cuts at:      0      12      72    1092    1373    2631 
   Size:         12      60    1020     281    1258

  Fragments arranged by size: 

                1258    1020     281      60      12
 
BstZ17I GTA'TAC

Cuts at:      0    1289    2631 
   Size:       1289    1342
 
BtgI C'CryG_G

Cuts at:      0     489    1034    1804    2390    2418    2631 
   Size:        489     545     770     586      28     213

  Fragments arranged by size: 

                 770     586     545     489     213      28
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     540    2631 
   Size:        540    2091
 
BtsI GCAGTG_nn'

Cuts at:      0     373    1411    2631 
   Size:        373    1038    1220

  Fragments arranged by size: 

                1220    1038     373
 
DraIII CAC_nnn'GTG

Cuts at:      0     575    1478    2631 
   Size:        575     903    1153

  Fragments arranged by size: 

                1153     903     575
 
EaeI y'GGCC_r

Cuts at:      0     726    1128    1302    1691    2196    2631 
   Size:        726     402     174     389     505     435

  Fragments arranged by size: 

                 726     505     435     402     389     174
 
EarI CTCTTCn'nnn_

Cuts at:      0      89     283    1361    2618    2631 
   Size:         89     194    1078    1257      13

  Fragments arranged by size: 

                1257    1078     194      89      13
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1528    2631 
   Size:       1528    1103
 
EcoICRI GAG'CTC

Cuts at:      0    1665    2505    2562    2631 
   Size:       1665     840      57      69

  Fragments arranged by size: 

                1665     840      69      57
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     516     643     955    1084    1187    1237    1885 
   Size:        113     403     127     312     129     103      50     648

Cuts at:   1885    1942    2299    2498    2599    2631 
   Size:         57     357     199     101      32

  Fragments arranged by size: 

                 648     403     357     312     199     129     127     113
                 103     101      57      50      32
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1507    2631 
   Size:       1507    1124
 
EcoO109I rG'GnC_Cy

Cuts at:      0       5     657    1707    1788    1854    2047    2631 
   Size:          5     652    1050      81      66     193     584

  Fragments arranged by size: 

                1050     652     584     193      81      66       5
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2629    2631 
   Size:       2629       2
 
HaeII r_GCGC'y

Cuts at:      0     630    2631 
   Size:        630    2001
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      29     699     731    1393    1425    2094    2126    2206 
   Size:         29     670      32     662      32     669      32      80

Cuts at:   2206    2238    2631 
   Size:         32     393

  Fragments arranged by size: 

                 670     669     662     393      80      32      32      32
                  32      29
 
KpnI G_GTAC'C

Cuts at:      0     363     378    1728    2631 
   Size:        363      15    1350     903

  Fragments arranged by size: 

                1350     903     363      15
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1003    2631 
   Size:       1003    1628
 
MscI TGG'CCA

Cuts at:      0     728    1130    1304    1693    2631 
   Size:        728     402     174     389     938

  Fragments arranged by size: 

                 938     728     402     389     174
 
MslI CAynn'nnrTG

Cuts at:      0     415    1622    2311    2631 
   Size:        415    1207     689     320

  Fragments arranged by size: 

                1207     689     415     320
 
MspA1I CmG'CkG

Cuts at:      0     201     647    1113    2083    2631 
   Size:        201     446     466     970     548

  Fragments arranged by size: 

                 970     548     466     446     201
 
NcoI C'CATG_G

Cuts at:      0    1804    2390    2418    2631 
   Size:       1804     586      28     213

  Fragments arranged by size: 

                1804     586     213      28
 
NdeI CA'TA_TG

Cuts at:      0    1608    2631 
   Size:       1608    1023
 
NsiI A_TGCA'T

Cuts at:      0    1824    2631 
   Size:       1824     807
 
NspI r_CATG'y

Cuts at:      0    1242    1530    1599    1932    2332    2631 
   Size:       1242     288      69     333     400     299

  Fragments arranged by size: 

                1242     400     333     299     288      69
 
PasI CC'CwG_GG

Cuts at:      0     904    2631 
   Size:        904    1727
 
PciI A'CATG_T

Cuts at:      0    1526    1928    2328    2631 
   Size:       1526     402     400     303

  Fragments arranged by size: 

                1526     402     400     303
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2331    2555    2631 
   Size:       2331     224      76

  Fragments arranged by size: 

                2331     224      76
 
PfoI T'CCnGG_A

Cuts at:      0    2132    2631 
   Size:       2132     499
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1793    1825    2631 
   Size:       1793      32     806

  Fragments arranged by size: 

                1793     806      32
 
PpuMI rG'GwC_Cy

Cuts at:      0       5    1707    1788    1854    2631 
   Size:          5    1702      81      66     777

  Fragments arranged by size: 

                1702     777      81      66       5
 
PshAI GACnn'nnGTC

Cuts at:      0    1559    2631 
   Size:       1559    1072
 
PspXI vC'TCGA_Gb

Cuts at:      0    1199    2631 
   Size:       1199    1432
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1279    1311    2631 
   Size:       1279      32    1320

  Fragments arranged by size: 

                1320    1279      32
 
PstI C_TGCA'G

Cuts at:      0     368    1880    2631 
   Size:        368    1512     751

  Fragments arranged by size: 

                1512     751     368
 
PvuII CAG'CTG

Cuts at:      0     201     647    1113    2083    2631 
   Size:        201     446     466     970     548

  Fragments arranged by size: 

                 970     548     466     446     201
 
SacI G_AGCT'C

Cuts at:      0    1667    2507    2564    2631 
   Size:       1667     840      57      67

  Fragments arranged by size: 

                1667     840      67      57
 
SanDI GG'GwC_CC

Cuts at:      0       5    1788    2631 
   Size:          5    1783     843

  Fragments arranged by size: 

                1783     843       5
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2618    2631 
   Size:       2618      13
 
SexAI A'CCwGG_T

Cuts at:      0     236    2631 
   Size:        236    2395
 
SfcI C'TryA_G

Cuts at:      0     364     800    1278    1672    1876    2631 
   Size:        364     436     478     394     204     755

  Fragments arranged by size: 

                 755     478     436     394     364     204
 
SmlI C'TyrA_G

Cuts at:      0     354    1199    1492    1576    1660    2631 
   Size:        354     845     293      84      84     971

  Fragments arranged by size: 

                 971     845     354     293      84      84
 
StuI AGG'CCT

Cuts at:      0    2284    2374    2631 
   Size:       2284      90     257

  Fragments arranged by size: 

                2284     257      90
 
StyI C'CwwG_G

Cuts at:      0     110     458    1804    2292    2390    2418    2631 
   Size:        110     348    1346     488      98      28     213

  Fragments arranged by size: 

                1346     488     348     213     110      98      28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     451     777     874    2025    2451    2455    2631 
   Size:        451     326      97    1151     426       4     176

  Fragments arranged by size: 

                1151     451     426     326     176      97       4
 
TatI w'GTAC_w

Cuts at:      0    1598    2631 
   Size:       1598    1033
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     797     829    1460    1492    2114    2146    2631 
   Size:        797      32     631      32     622      32     485

  Fragments arranged by size: 

                 797     631     622     485      32      32      32
 
XbaI T'CTAG_A

Cuts at:      0     846    2631 
   Size:        846    1785
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     911    2026    2598    2631 
   Size:        911    1115     572      33

  Fragments arranged by size: 

                1115     911     572      33
 
XhoI C'TCGA_G

Cuts at:      0    1199    2631 
   Size:       1199    1432
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AleI     AloI    AlwNI
     AvaI    AvrII    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
     BclI    BfrBI     BglI     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I
     BtgI    BtgZI     BtsI   DraIII     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I     KpnI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NsiI     NspI     PasI
     PciI    PflMI     PfoI     PpiI    PpuMI    PshAI    PspXI     PsrI
     PstI    PvuII     SacI    SanDI     SapI    SexAI     SfcI     SmlI
     StuI     StyI    TaqII     TatI     TstI     XbaI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AhdI     AlfI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI     BbeI    BciVI
    BglII    BmgBI     BmtI     BsaI    BsaAI    BsaBI    BsiWI     BsmI
    BsrBI   BssHII    BstBI   Bsu36I     ClaI    CspCI     DraI     DrdI
     EagI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     MfeI     MluI     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     PacI     PmeI     PmlI     PsiI   PspOMI     PvuI
    RsrII    SacII     SalI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
  Tth111I     XmaI     XmnI     ZraI
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