Restriction map for pF1KSDA1599
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       Acc65I      AccIII      AflII       AgeI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AvrII       BamHI       BbeI        BbsI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BpuAI       BsaBI       BsaI        
BsaWI       BseAI       BsiWI       Bsp1407I    BspDI       
BspEI       BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BstAPI      BstBI       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EarI        Eco47III    EcoRV       EcoT22I     FbaI        
FseI        HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MamI        MfeI        
MluI        MroI        MseI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshBI       RsrII       SacII       
SalI        SapI        SexAI       SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
SspBI       SspI        SwaI        TfiI        Tru9I       
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI          147  GTATAC               GT^[AC][GT]AC
AclI          630  AACGTT               AA^CGTT
AflIII        113  ACATGT               A^C[GA][CT]GT
AhdI           16  GACATGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        942  GTGCAC               G^TGCAC
AlwNI         516  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI           10  CCTGAGG              CC^T[ACGT]AGG
ApaLI         942  GTGCAC               G^TGCAC
Asp700I       570  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI          16  GACATGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1552  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI          487  GGCACC               G^G[CT][GA]CC
BbvCI         505  GCTGAGG              CCTCAGC(-5/-2)
BglI         1618  GCCCGCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI         1246  CTGGAG               CTGGAG(16/14)
BsmAI        1631  GAGAC                GTCTC(1/5)
BsmBI        1631  GAGACG               CGTCTC(1/5)
BspHI         659  TCATGA               T^CATGA
BspLU11I      113  ACATGT               A^CATGT
BssSI        1186  CACGAG               CACGAG(-5/-1)
Bst1107I      147  GTATAC               GTA^TAC
BstEII       1311  GGTCACC              G^GT[ACGT]ACC
BstPI        1311  GGTCACC              G^GT[ACGT]ACC
BstZ17I       147  GTATAC               GTA^TAC
Bsu36I         10  CCTGAGG              CC^T[ACGT]AGG
EagI         1337  CGGCCG               C^GGCCG
Eam1105I       16  GACATGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1337  CGGCCG               C^GGCCG
Eco52I       1337  CGGCCG               C^GGCCG
Eco81I         10  CCTGAGG              CC^T[ACGT]AGG
EcoNI         333  CCTGGGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1311  GGTCACC              G^GT[ACGT]ACC
HaeII        1083  GGCGCT               [GA]GCGC^[CT]
NcoI         1490  CCATGG               C^CATGG
NspI          113  ACATGT               [GA]CATG^[CT]
PflFI        1552  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I      630  AACGTT               AA^CGTT
PstI           90  CTGCAG               CTGCA^G
PvuI          732  CGATCG               CGAT^CG
RcaI          659  TCATGA               T^CATGA
SacI          511  GAGCTC               GAGCT^C
SbfI           89  CCTGCAGG             CCTGCA^GG
ScaI         1358  AGTACT               AGT^ACT
SfiI         1617  GGCCCGCCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         1529  CCCGGG               CCC^GGG
Sse8387I       89  CCTGCAGG             CCTGCA^GG
Tth111I      1552  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        1529  CCCGGG               C^CCGGG
XmaI         1529  CCCGGG               C^CCGGG
XmnI          570  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcsI          423  GAATTC               [GA]^AATT[CT]
              897  GAATTC              
AcyI           21  GGCGTC               G[GA]^CG[CT]C
             1486  GACGCC              
ApoI          423  GAATTC               [GA]^AATT[CT]
              897  GAATTC              
AspHI         511  GAGCTC               G[AT]GC[AT]^C
              942  GTGCAC              
AvaI         1529  CCCGGG               C^[CT]CG[GA]G
             1628  CCCGAG              
AviII         230  TGCGCA               TGC^GCA
             1268  TGCGCA              
BalI          992  TGGCCA               TGG^CCA
             1322  TGGCCA              
BbrPI        1318  CACGTG               CAC^GTG
             1603  CACGTG              
BsaHI          21  GGCGTC               G[GA]^CG[CT]C
             1486  GACGCC              
BsiEI         732  CGATCG               CG[GA][CT]^CG
             1337  CGGCCG              
BsiHKAI       511  GAGCTC               G[AT]GC[AT]^C
              942  GTGCAC              
BsoBI        1529  CCCGGG               C^[CT]CG[GA]G
             1628  CCCGAG              
BstYI        1520  GGATCT               [GA]^GATC[CT]
             1646  AGATCC              
DraII         370  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              970  GGGACCC             
EcoO109I      370  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              970  GGGACCC             
EcoRI         423  GAATTC               G^AATTC
              897  GAATTC              
FspI          230  TGCGCA               TGC^GCA
             1268  TGCGCA              
Hin1I          21  GGCGTC               G[GA]^CG[CT]C
             1486  GACGCC              
HincII        295  GTTGAC               GT[CT]^[GA]AC
             1426  GTCAAC              
HindII        295  GTTGAC               GT[CT]^[GA]AC
             1426  GTCAAC              
MboII         663  GAAGA                GAAGA(8/7)
              879  GAAGA               
MflI         1520  GGATCT               [GA]^GATC[CT]
             1646  AGATCC              
MluNI         992  TGGCCA               TGG^CCA
             1322  TGGCCA              
MscI          992  TGGCCA               TGG^CCA
             1322  TGGCCA              
PflMI         153  CCAAGGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1195  CCACTGAATGG         
PmaCI        1318  CACGTG               CAC^GTG
             1603  CACGTG              
PmlI         1318  CACGTG               CAC^GTG
             1603  CACGTG              
PpuMI         370  GGGTCCC              [GA]G^G[AT]CC[CT]
              970  GGGACCC             
PshAI         651  GACCGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1089  GACTGCCGTC          
StuI          341  AGGCCT               AGG^CCT
             1250  AGGCCT              
Van91I        153  CCAAGGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1195  CCACTGAATGG         
XcmI          780  CCATGACTTCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1490  CCATGGTGGAGCTGG     
XhoII        1520  GGATCT               [GA]^GATC[CT]
             1646  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AvaII       BanII       BbvI        BcnI        BlpI        
BmyI        Bpu1102I    BsaAI       BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmFI       BsmI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DsaI        EaeI        
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
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