Restriction map for pF1KSDA1599
 (Linear) (Six-base) MAPSORT of: KIAA1599.seq  Check: 3306  from: 1  to: 1779



KIAA1599.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AccI GT'mk_AC

Cuts at:      0     148    1779 
   Size:        148    1631
 
AclI AA'CG_TT

Cuts at:      0     631    1779 
   Size:        631    1148
 
AflIII A'CryG_T

Cuts at:      0     113    1779 
   Size:        113    1666
 
AhdI GACnn_n'nnGTC

Cuts at:      0      21    1779 
   Size:         21    1758
 
AleI CACnn'nnGTG

Cuts at:      0      70    1318    1779 
   Size:         70    1248     461

  Fragments arranged by size: 

                1248     461      70
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     460     566    1621    1779 
   Size:        460     106    1055     158

  Fragments arranged by size: 

                1055     460     158     106
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     616     648    1779 
   Size:        616      32    1131

  Fragments arranged by size: 

                1131     616      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     521    1779 
   Size:        521    1258
 
ApaLI G'TGCA_C

Cuts at:      0     942    1779 
   Size:        942     837
 
ApoI r'AATT_y

Cuts at:      0     423     897    1779 
   Size:        423     474     882

  Fragments arranged by size: 

                 882     474     423
 
AvaI C'yCGr_G

Cuts at:      0    1529    1628    1779 
   Size:       1529      99     151

  Fragments arranged by size: 

                1529     151      99
 
BanI G'GyrC_C

Cuts at:      0     487    1779 
   Size:        487    1292
 
BanII G_rGCy'C

Cuts at:      0     515     714    1057    1779 
   Size:        515     199     343     722

  Fragments arranged by size: 

                 722     515     343     199
 
BbvCI CC'TCA_GC

Cuts at:      0     506    1779 
   Size:        506    1273
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1624    1779 
   Size:       1624     155
 
BlpI GC'TnA_GC

Cuts at:      0      28     514    1609    1779 
   Size:         28     486    1095     170

  Fragments arranged by size: 

                1095     486     170      28
 
Bme1580I G_kGCm'C

Cuts at:      0     946    1779 
   Size:        946     833
 
BmgBI CAC'GTC

Cuts at:      0    1515    1635    1779 
   Size:       1515     120     144

  Fragments arranged by size: 

                1515     144     120
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     771    1350    1779 
   Size:        771     579     429

  Fragments arranged by size: 

                 771     579     429
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      57    1236    1779 
   Size:         57    1179     543

  Fragments arranged by size: 

                1179     543      57
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1267    1779 
   Size:       1267     512
 
Bpu10I CC'TnA_GC

Cuts at:      0     506    1779 
   Size:        506    1273
 
BsaAI yAC'GTr

Cuts at:      0     894    1320    1584    1605    1779 
   Size:        894     426     264      21     174

  Fragments arranged by size: 

                 894     426     264     174      21
 
BsaHI Gr'CG_yC

Cuts at:      0      22    1487    1779 
   Size:         22    1465     292

  Fragments arranged by size: 

                1465     292      22
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     350     380     925     955    1779 
   Size:        350      30     545      30     824

  Fragments arranged by size: 

                 824     545     350      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     274     523    1515    1779 
   Size:        274     249     992     264

  Fragments arranged by size: 

                 992     274     264     249
 
BseYI C'CCAG_C

Cuts at:      0    1737    1779 
   Size:       1737      42
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     333    1027    1297    1363    1750    1779 
   Size:        333     694     270      66     387      29

  Fragments arranged by size: 

                 694     387     333     270      66      29
 
BsiEI CG_ry'CG

Cuts at:      0     735    1340    1779 
   Size:        735     605     439

  Fragments arranged by size: 

                 735     605     439
 
BsiHKAI G_wGCw'C

Cuts at:      0     515     946    1779 
   Size:        515     431     833

  Fragments arranged by size: 

                 833     515     431
 
BsmI GAATG_Cn'

Cuts at:      0     412    1335    1685    1779 
   Size:        412     923     350      94

  Fragments arranged by size: 

                 923     412     350      94
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1625    1779 
   Size:       1625     154
 
Bsp1286I G_dGCh'C

Cuts at:      0     515     714     946    1057    1779 
   Size:        515     199     232     111     722

  Fragments arranged by size: 

                 722     515     232     199     111
 
BspHI T'CATG_A

Cuts at:      0     659    1779 
   Size:        659    1120
 
BssSI C'ACGA_G

Cuts at:      0    1186    1779 
   Size:       1186     593
 
BstEII G'GTnAC_C

Cuts at:      0    1311    1779 
   Size:       1311     468
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      72     561     747    1779 
   Size:         72     489     186    1032

  Fragments arranged by size: 

                1032     489     186      72
 
BstYI r'GATC_y

Cuts at:      0    1520    1646    1779 
   Size:       1520     126     133

  Fragments arranged by size: 

                1520     133     126
 
BstZ17I GTA'TAC

Cuts at:      0     149    1779 
   Size:        149    1630
 
Bsu36I CC'TnA_GG

Cuts at:      0      11    1779 
   Size:         11    1768
 
BtgI C'CryG_G

Cuts at:      0     825    1307    1490    1779 
   Size:        825     482     183     289

  Fragments arranged by size: 

                 825     482     289     183
 
BtsI GCAGTG_nn'

Cuts at:      0     178    1004    1779 
   Size:        178     826     775

  Fragments arranged by size: 

                 826     775     178
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1281    1316    1779 
   Size:       1281      35     463

  Fragments arranged by size: 

                1281     463      35
 
EaeI y'GGCC_r

Cuts at:      0      62     992    1322    1337    1779 
   Size:         62     930     330      15     442

  Fragments arranged by size: 

                 930     442     330      62      15
 
EagI C'GGCC_G

Cuts at:      0    1337    1779 
   Size:       1337     442
 
EcoICRI GAG'CTC

Cuts at:      0     513    1779 
   Size:        513    1266
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1267    1779 
   Size:       1267     512
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     337    1779 
   Size:        337    1442
 
EcoO109I rG'GnC_Cy

Cuts at:      0     371     971    1779 
   Size:        371     600     808

  Fragments arranged by size: 

                 808     600     371
 
EcoRI G'AATT_C

Cuts at:      0     423     897    1779 
   Size:        423     474     882

  Fragments arranged by size: 

                 882     474     423
 
FspI TGC'GCA

Cuts at:      0     232    1270    1779 
   Size:        232    1038     509

  Fragments arranged by size: 

                1038     509     232
 
HaeII r_GCGC'y

Cuts at:      0    1087    1779 
   Size:       1087     692
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       7      39     283     315    1091    1123    1779 
   Size:          7      32     244      32     776      32     656

  Fragments arranged by size: 

                 776     656     244      32      32      32       7
 
HincII GTy'rAC

Cuts at:      0     297    1428    1779 
   Size:        297    1131     351

  Fragments arranged by size: 

                1131     351     297
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     482    1077    1779 
   Size:        152     330     595     702

  Fragments arranged by size: 

                 702     595     330     152
 
MscI TGG'CCA

Cuts at:      0     994    1324    1779 
   Size:        994     330     455

  Fragments arranged by size: 

                 994     455     330
 
MslI CAynn'nnrTG

Cuts at:      0      70     703     745    1318    1779 
   Size:         70     633      42     573     461

  Fragments arranged by size: 

                 633     573     461      70      42
 
MspA1I CmG'CkG

Cuts at:      0     451     505    1065    1281    1336    1779 
   Size:        451      54     560     216      55     443

  Fragments arranged by size: 

                 560     451     443     216      55      54
 
NcoI C'CATG_G

Cuts at:      0    1490    1779 
   Size:       1490     289
 
NspI r_CATG'y

Cuts at:      0     117    1779 
   Size:        117    1662
 
PasI CC'CwG_GG

Cuts at:      0     376    1143    1779 
   Size:        376     767     636

  Fragments arranged by size: 

                 767     636     376
 
PciI A'CATG_T

Cuts at:      0     113    1779 
   Size:        113    1666
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     159    1201    1779 
   Size:        159    1042     578

  Fragments arranged by size: 

                1042     578     159
 
PfoI T'CCnGG_A

Cuts at:      0     101     427    1244    1574    1779 
   Size:        101     326     817     330     205

  Fragments arranged by size: 

                 817     330     326     205     101
 
PmlI CAC'GTG

Cuts at:      0    1320    1605    1779 
   Size:       1320     285     174

  Fragments arranged by size: 

                1320     285     174
 
PpuMI rG'GwC_Cy

Cuts at:      0     371     971    1779 
   Size:        371     600     808

  Fragments arranged by size: 

                 808     600     371
 
PshAI GACnn'nnGTC

Cuts at:      0     655    1093    1779 
   Size:        655     438     686

  Fragments arranged by size: 

                 686     655     438
 
PstI C_TGCA'G

Cuts at:      0      94    1779 
   Size:         94    1685
 
PvuI CG_AT'CG

Cuts at:      0     735    1779 
   Size:        735    1044
 
PvuII CAG'CTG

Cuts at:      0     451    1065    1281    1779 
   Size:        451     614     216     498

  Fragments arranged by size: 

                 614     498     451     216
 
SacI G_AGCT'C

Cuts at:      0     515    1779 
   Size:        515    1264
 
SanDI GG'GwC_CC

Cuts at:      0     371     971    1779 
   Size:        371     600     808

  Fragments arranged by size: 

                 808     600     371
 
SbfI CC_TGCA'GG

Cuts at:      0      94    1779 
   Size:         94    1685
 
ScaI AGT'ACT

Cuts at:      0    1360    1779 
   Size:       1360     419
 
SfcI C'TryA_G

Cuts at:      0      90     522     609    1779 
   Size:         90     432      87    1170

  Fragments arranged by size: 

                1170     432      90      87
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1624    1779 
   Size:       1624     155
 
SmaI CCC'GGG

Cuts at:      0    1531    1779 
   Size:       1531     248
 
StuI AGG'CCT

Cuts at:      0     343    1252    1779 
   Size:        343     909     527

  Fragments arranged by size: 

                 909     527     343
 
StyI C'CwwG_G

Cuts at:      0      44      68     153     354    1412    1490    1779 
   Size:         44      24      85     201    1058      78     289

  Fragments arranged by size: 

                1058     289     201      85      78      44      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     667    1330    1779 
   Size:        667     663     449

  Fragments arranged by size: 

                 667     663     449
 
TatI w'GTAC_w

Cuts at:      0    1358    1779 
   Size:       1358     421
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     201     233     350     382     660     692     925     957 
   Size:        201      32     117      32     278      32     233      32

Cuts at:    957    1779 
   Size:        822

  Fragments arranged by size: 

                 822     278     233     201     117      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1555    1779 
   Size:       1555     224
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     787    1497    1779 
   Size:        787     710     282

  Fragments arranged by size: 

                 787     710     282
 
XmaI C'CCGG_G

Cuts at:      0    1529    1779 
   Size:       1529     250
 
XmnI GAAnn'nnTTC

Cuts at:      0     574    1779 
   Size:        574    1205
 

 Enzymes that do cut:

     AccI     AclI   AflIII     AhdI     AleI     AlfI     AloI    AlwNI
    ApaLI     ApoI     AvaI     BanI    BanII    BbvCI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BsaAI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BssSI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI     BtsI    CspCI     EaeI     EagI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII     MmeI     MscI
     MslI   MspA1I     NcoI     NspI     PasI     PciI    PflMI     PfoI
     PmlI    PpuMI    PshAI     PstI     PvuI    PvuII     SacI    SanDI
     SbfI     ScaI     SfcI     SfiI     SmaI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AcuI     AfeI    AflII     AgeI     ApaI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI    BglII     BmtI    BpuEI     BsaI
    BsaBI    BsaWI    BsiWI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI    BtgZI     ClaI     DraI   DraIII
     DrdI     EarI     EciI    EcoRV     FalI     FseI    FspAI  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PmeI     PpiI
     PsiI   PspOMI    PspXI     PsrI    RsrII    SacII     SalI     SapI
    SexAI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     XbaI     XhoI     ZraI
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