Restriction map for pF1KSDA1589
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

Acc65I      AclI        AflIII      AhdI        AocI        
Aor51HI     AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BamHI       BbeI        
BbrPI       BcgI        BglII       BlnI        BsaAI       
BsiEI       BsiWI       BsmBI       BsmI        BspLU11I    
BssHII      BstBI       BstXI       Bsu36I      CpoI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoRI       EcoRV       EcoT22I     FseI        
FspI        HincII      HindII      HpaI        KasI        
KpnI        KspI        MfeI        MluI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SapI        SbfI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        XbaI        
XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AatII        1824  GACGTC               GACGT^C
AccII        2115  CGCG                 CG^CG
AccIII       1031  TCCGGA               T^CCGGA
AcyI         1824  GACGTC               G[GA]^CG[CT]C
AflII         760  CTTAAG               C^TTAAG
AgeI         1025  ACCGGT               A^CCGGT
Alw44I        964  GTGCAC               G^TGCAC
ApaLI         964  GTGCAC               G^TGCAC
AspI          781  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1176  CCCGGG               C^[CT]CG[GA]G
BbsI          496  GAAGAC               GAAGAC(2/6)
BbvCI        1299  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1593  GGATAC               GTATCC(6/5)
BfrI          760  CTTAAG               C^TTAAG
BglI         1195  GCCCTGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1719  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     1719  GCTTAGC              GC^T[ACGT]AGC
BpuAI         496  GAAGAC               GAAGAC(2/6)
BsaBI         529  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1824  GACGTC               G[GA]^CG[CT]C
BseAI        1031  TCCGGA               T^CCGGA
BsoBI        1176  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      359  TGTACA               T^GTACA
BspDI         700  ATCGAT               AT^CGAT
BspEI        1031  TCCGGA               T^CCGGA
BspHI        1356  TCATGA               T^CATGA
BsrDI        2028  CATTGC               GCAATG(2/0)
BsrGI         359  TGTACA               T^GTACA
BssSI        2270  CTCGTG               CACGAG(-5/-1)
Bst1107I     1407  GTATAC               GTA^TAC
BstEII       2323  GGTGACC              G^GT[ACGT]ACC
BstPI        2323  GGTGACC              G^GT[ACGT]ACC
BstUI        2115  CGCG                 CG^CG
BstZ17I      1407  GTATAC               GTA^TAC
CelII        1719  GCTTAGC              GC^T[ACGT]AGC
ClaI          700  ATCGAT               AT^CGAT
DraIII       1205  CACTAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      2323  GGTGACC              G^GT[ACGT]ACC
HgaI         2113  GACGC                GACGC(5/10)
Hin1I        1824  GACGTC               G[GA]^CG[CT]C
HindIII       854  AAGCTT               A^AGCTT
MamI          529  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1031  TCCGGA               T^CCGGA
MslI         1387  CACCAGTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI         2115  CGCG                 CG^CG
NspI         1620  GCATGT               [GA]CATG^[CT]
PflFI         781  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         972  CCAGCTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1025  ACCGGT               A^CCGGT
PpuMI         764  AGGTCCT              [GA]G^G[AT]CC[CT]
RcaI         1356  TCATGA               T^CATGA
ScaI         1565  AGTACT               AGT^ACT
SexAI         243  ACCAGGT              A^CC[AT]GGT
SmaI         1176  CCCGGG               CCC^GGG
SspBI         359  TGTACA               T^GTACA
Tth111I       781  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        972  CCAGCTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        1176  CCCGGG               C^CCGGG
XmaI         1176  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI          460  GTAGAC               GT^[AC][GT]AC
             1407  GTATAC              
AcsI          476  AAATTC               [GA]^AATT[CT]
              832  AAATTC              
AlwI         1230  GATCC                GGATC(4/5)
             1585  GATCC               
ApaI          933  GGGCCC               GGGCC^C
             1929  GGGCCC              
ApoI          476  AAATTC               [GA]^AATT[CT]
              832  AAATTC              
BalI          195  TGGCCA               TGG^CCA
              770  TGGCCA              
BclI          528  TGATCA               T^GATCA
              987  TGATCA              
BsaWI        1025  ACCGGT               [AT]^CCGG[AT]
             1031  TCCGGA              
BsgI          648  CTGCAC               GTGCAG(16/14)
             2053  GTGCAG              
BsrBI         175  GAGCGG               CCGCTC(-3/-3)
             1918  GAGCGG              
BsrFI         809  ACCGGC               [GA]^CCGG[CT]
             1025  ACCGGT              
BstAPI       1415  GCAAGGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1498  GCAAAATATGC         
BstYI        1229  AGATCC               [GA]^GATC[CT]
             1584  AGATCC              
Cfr10I        809  ACCGGC               [GA]^CCGG[CT]
             1025  ACCGGT              
DraI          622  TTTAAA               TTT^AAA
             1831  TTTAAA              
DrdI          636  GACCCAGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2281  GACCATCCCGTC        
DsaI         1103  CCACGG               C^C[GA][CT]GG
             2072  CCGTGG              
EcoNI         216  CCTCTGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1778  CCTACTGGAGG         
FbaI          528  TGATCA               T^GATCA
              987  TGATCA              
HaeII         158  AGCGCC               [GA]GCGC^[CT]
             1673  AGCGCC              
MaeII        1825  ACGT                 A^CGT
             1976  ACGT                
MflI         1229  AGATCC               [GA]^GATC[CT]
             1584  AGATCC              
MluNI         195  TGGCCA               TGG^CCA
              770  TGGCCA              
MscI          195  TGGCCA               TGG^CCA
              770  TGGCCA              
PvuII        1302  CAGCTG               CAG^CTG
             1887  CAGCTG              
SacI          142  GAGCTC               GAGCT^C
              880  GAGCTC              
SmlI          760  CTTAAG               C^T[CT][GA]AG
              883  CTCAAG              
StuI         1418  AGGCCT               AGG^CCT
             2122  AGGCCT              
XhoII        1229  AGATCC               [GA]^GATC[CT]
             1584  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       Asp700I     
AspHI       AvaII       BanI        BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MnlI        MseI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XmnI        
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