Restriction map for pF1KSDA1589
 (Linear) (Six-base) MAPSORT of: KIAA1589.seq  Check: 9309  from: 1  to: 2331



KIAA1589.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1291    2331 
   Size:       1291    1040
 
AatII G_ACGT'C

Cuts at:      0    1828    2331 
   Size:       1828     503
 
AccI GT'mk_AC

Cuts at:      0     461    1408    2331 
   Size:        461     947     923

  Fragments arranged by size: 

                 947     923     461
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     883    1073    1203    1219    1669    2331 
   Size:        883     190     130      16     450     662

  Fragments arranged by size: 

                 883     662     450     190     130      16
 
AflII C'TTAA_G

Cuts at:      0     760    2331 
   Size:        760    1571
 
AgeI A'CCGG_T

Cuts at:      0    1025    2331 
   Size:       1025    1306
 
AlwNI CAG_nnn'CTG

Cuts at:      0     537     978    1677    1803    1915    2331 
   Size:        537     441     699     126     112     416

  Fragments arranged by size: 

                 699     537     441     416     126     112
 
ApaI G_GGCC'C

Cuts at:      0     937    1933    2331 
   Size:        937     996     398

  Fragments arranged by size: 

                 996     937     398
 
ApaLI G'TGCA_C

Cuts at:      0     964    2331 
   Size:        964    1367
 
ApoI r'AATT_y

Cuts at:      0     476     832    2331 
   Size:        476     356    1499

  Fragments arranged by size: 

                1499     476     356
 
AvaI C'yCGr_G

Cuts at:      0    1176    2331 
   Size:       1176    1155
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1586    1619    2331 
   Size:       1586      33     712

  Fragments arranged by size: 

                1586     712      33
 
BanI G'GyrC_C

Cuts at:      0     272    1002    1172    2136    2331 
   Size:        272     730     170     964     195

  Fragments arranged by size: 

                 964     730     272     195     170
 
BanII G_rGCy'C

Cuts at:      0     146     716     884     937     985    1933    2331 
   Size:        146     570     168      53      48     948     398

  Fragments arranged by size: 

                 948     570     398     168     146      53      48
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     503    2331 
   Size:        503    1828
 
BbvCI CC'TCA_GC

Cuts at:      0    1300    2331 
   Size:       1300    1031
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1587    2331 
   Size:       1587     744
 
BclI T'GATC_A

Cuts at:      0     528     987    2331 
   Size:        528     459    1344

  Fragments arranged by size: 

                1344     528     459
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1201    2331 
   Size:       1201    1130
 
BlpI GC'TnA_GC

Cuts at:      0    1720    2331 
   Size:       1720     611
 
Bme1580I G_kGCm'C

Cuts at:      0       9     937     968    1457    1933    2331 
   Size:          9     928      31     489     476     398

  Fragments arranged by size: 

                 928     489     476     398      31       9
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     673     940     988    2142    2331 
   Size:        673     267      48    1154     189

  Fragments arranged by size: 

                1154     673     267     189      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     361     409    1803    2331 
   Size:        322      39      48    1394     528

  Fragments arranged by size: 

                1394     528     322      48      39
 
Bpu10I CC'TnA_GC

Cuts at:      0    1300    1916    2331 
   Size:       1300     616     415

  Fragments arranged by size: 

                1300     616     415
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     868    2331 
   Size:        868    1463
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     232     406     952    1498    2331 
   Size:        232     174     546     546     833

  Fragments arranged by size: 

                 833     546     546     232     174
 
BsaBI GATnn'nnATC

Cuts at:      0     533    2331 
   Size:        533    1798
 
BsaHI Gr'CG_yC

Cuts at:      0    1825    2331 
   Size:       1825     506
 
BsaWI w'CCGG_w

Cuts at:      0    1025    1031    2331 
   Size:       1025       6    1300

  Fragments arranged by size: 

                1300    1025       6
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1149    1179    2060    2090    2331 
   Size:       1149      30     881      30     241

  Fragments arranged by size: 

                1149     881     241      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     151     154     424    1278    2331 
   Size:        151       3     270     854    1053

  Fragments arranged by size: 

                1053     854     270     151       3
 
BseYI C'CCAG_C

Cuts at:      0      18     971    1038    1670    1925    2276    2331 
   Size:         18     953      67     632     255     351      55

  Fragments arranged by size: 

                 953     632     351     255      67      55      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     633    2074    2331 
   Size:        633    1441     257

  Fragments arranged by size: 

                1441     633     257
 
BsiHKAI G_wGCw'C

Cuts at:      0     146     884     968    2245    2331 
   Size:        146     738      84    1277      86

  Fragments arranged by size: 

                1277     738     146      86      84
 
Bsp1286I G_dGCh'C

Cuts at:      0       9     146     716     884     937     968     985    1457 
   Size:          9     137     570     168      53      31      17     472

Cuts at:   1457    1933    2245    2331 
   Size:        476     312      86

  Fragments arranged by size: 

                 570     476     472     312     168     137      86      53
                  31      17       9
 
BspEI T'CCGG_A

Cuts at:      0    1031    2331 
   Size:       1031    1300
 
BspHI T'CATG_A

Cuts at:      0    1356    2331 
   Size:       1356     975
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     289    1291    1420    2331 
   Size:        289    1002     129     911

  Fragments arranged by size: 

                1002     911     289     129
 
BsrBI CCG'CTC

Cuts at:      0     177    1920    2331 
   Size:        177    1743     411

  Fragments arranged by size: 

                1743     411     177
 
BsrDI GCAATG_nn'

Cuts at:      0    2027    2331 
   Size:       2027     304
 
BsrFI r'CCGG_y

Cuts at:      0     809    1025    2331 
   Size:        809     216    1306

  Fragments arranged by size: 

                1306     809     216
 
BsrGI T'GTAC_A

Cuts at:      0     359    2331 
   Size:        359    1972
 
BssSI C'ACGA_G

Cuts at:      0    2270    2331 
   Size:       2270      61
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1421    1504    2331 
   Size:       1421      83     827

  Fragments arranged by size: 

                1421     827      83
 
BstEII G'GTnAC_C

Cuts at:      0    2323    2331 
   Size:       2323       8
 
BstYI r'GATC_y

Cuts at:      0    1229    1584    2331 
   Size:       1229     355     747

  Fragments arranged by size: 

                1229     747     355
 
BstZ17I GTA'TAC

Cuts at:      0    1409    2331 
   Size:       1409     922
 
BtgI C'CryG_G

Cuts at:      0    1103    2072    2331 
   Size:       1103     969     259

  Fragments arranged by size: 

                1103     969     259
 
BtsI GCAGTG_nn'

Cuts at:      0     127     701     893    1854    2157    2205    2331 
   Size:        127     574     192     961     303      48     126

  Fragments arranged by size: 

                 961     574     303     192     127     126      48
 
ClaI AT'CG_AT

Cuts at:      0     701    2331 
   Size:        701    1630
 
DraI TTT'AAA

Cuts at:      0     624    1833    2331 
   Size:        624    1209     498

  Fragments arranged by size: 

                1209     624     498
 
DraIII CAC_nnn'GTG

Cuts at:      0    1210    2331 
   Size:       1210    1121
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     642    2287    2331 
   Size:        642    1645      44

  Fragments arranged by size: 

                1645     642      44
 
EaeI y'GGCC_r

Cuts at:      0     195     770    1906    2117    2331 
   Size:        195     575    1136     211     214

  Fragments arranged by size: 

                1136     575     214     211     195
 
EarI CTCTTCn'nnn_

Cuts at:      0     307     392     832    2331 
   Size:        307      85     440    1499

  Fragments arranged by size: 

                1499     440     307      85
 
EcoICRI GAG'CTC

Cuts at:      0     144     882    2331 
   Size:        144     738    1449

  Fragments arranged by size: 

                1449     738     144
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     361     409     883    1073    1203    1219    1669 
   Size:        322      39      48     474     190     130      16     450

Cuts at:   1669    1803    2331 
   Size:        134     528

  Fragments arranged by size: 

                 528     474     450     322     190     134     130      48
                  39      16
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     220    1782    2331 
   Size:        220    1562     549

  Fragments arranged by size: 

                1562     549     220
 
EcoO109I rG'GnC_Cy

Cuts at:      0      30     765     934    1929    1930    2331 
   Size:         30     735     169     995       1     401

  Fragments arranged by size: 

                 995     735     401     169      30       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      83    2331 
   Size:         83    2248
 
HaeII r_GCGC'y

Cuts at:      0     162    1677    2331 
   Size:        162    1515     654

  Fragments arranged by size: 

                1515     654     162
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     562     594     979    1011    1515    1547    2227    2259 
   Size:        562      32     385      32     504      32     680      32

Cuts at:   2259    2331 
   Size:         72

  Fragments arranged by size: 

                 680     562     504     385      72      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0     854    2331 
   Size:        854    1477
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1293    1661    2331 
   Size:       1293     368     670

  Fragments arranged by size: 

                1293     670     368
 
MscI TGG'CCA

Cuts at:      0     197     772    2331 
   Size:        197     575    1559

  Fragments arranged by size: 

                1559     575     197
 
MslI CAynn'nnrTG

Cuts at:      0    1391    2331 
   Size:       1391     940
 
MspA1I CmG'CkG

Cuts at:      0      74     131    1223    1304    1373    1889    2331 
   Size:         74      57    1092      81      69     516     442

  Fragments arranged by size: 

                1092     516     442      81      74      69      57
 
NspI r_CATG'y

Cuts at:      0    1624    2331 
   Size:       1624     707
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     978    2331 
   Size:        978    1353
 
PfoI T'CCnGG_A

Cuts at:      0     377    1019    2331 
   Size:        377     642    1312

  Fragments arranged by size: 

                1312     642     377
 
PpuMI rG'GwC_Cy

Cuts at:      0     765    2331 
   Size:        765    1566
 
PspOMI G'GGCC_C

Cuts at:      0     933    1929    2331 
   Size:        933     996     402

  Fragments arranged by size: 

                 996     933     402
 
PstI C_TGCA'G

Cuts at:      0     115     122     942    1378    2331 
   Size:        115       7     820     436     953

  Fragments arranged by size: 

                 953     820     436     115       7
 
PvuII CAG'CTG

Cuts at:      0    1304    1889    2331 
   Size:       1304     585     442

  Fragments arranged by size: 

                1304     585     442
 
SacI G_AGCT'C

Cuts at:      0     146     884    2331 
   Size:        146     738    1447

  Fragments arranged by size: 

                1447     738     146
 
ScaI AGT'ACT

Cuts at:      0    1567    2331 
   Size:       1567     764
 
SexAI A'CCwGG_T

Cuts at:      0     243    2331 
   Size:        243    2088
 
SfcI C'TryA_G

Cuts at:      0     111     118     938    1374    2331 
   Size:        111       7     820     436     957

  Fragments arranged by size: 

                 957     820     436     111       7
 
SmaI CCC'GGG

Cuts at:      0    1178    2331 
   Size:       1178    1153
 
SmlI C'TyrA_G

Cuts at:      0     760     883    2331 
   Size:        760     123    1448

  Fragments arranged by size: 

                1448     760     123
 
StuI AGG'CCT

Cuts at:      0    1420    2124    2331 
   Size:       1420     704     207

  Fragments arranged by size: 

                1420     704     207
 
StyI C'CwwG_G

Cuts at:      0     837     875    1725    1736    2331 
   Size:        837      38     850      11     595

  Fragments arranged by size: 

                 850     837     595      38      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     985    2124    2331 
   Size:        985    1139     207

  Fragments arranged by size: 

                1139     985     207
 
TatI w'GTAC_w

Cuts at:      0     359    1565    2331 
   Size:        359    1206     766

  Fragments arranged by size: 

                1206     766     359
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1149    1181    2060    2092    2331 
   Size:       1149      32     879      32     239

  Fragments arranged by size: 

                1149     879     239      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     784    2331 
   Size:        784    1547
 
XmaI C'CCGG_G

Cuts at:      0    1176    2331 
   Size:       1176    1155
 
XmnI GAAnn'nnTTC

Cuts at:      0     476     868    2179    2331 
   Size:        476     392    1311     152

  Fragments arranged by size: 

                1311     476     392     152
 
ZraI GAC'GTC

Cuts at:      0    1826    2331 
   Size:       1826     505
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI    AflII     AgeI    AlwNI     ApaI
    ApaLI     ApoI     AvaI     BaeI     BanI    BanII     BbsI    BbvCI
    BciVI     BclI     BglI     BlpI Bme1580I     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstYI  BstZ17I     BtgI
     BtsI     ClaI     DraI   DraIII     DrdI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I  HindIII     MmeI
     MscI     MslI   MspA1I     NspI    PflMI     PfoI    PpuMI   PspOMI
     PstI    PvuII     SacI     ScaI    SexAI     SfcI     SmaI     SmlI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI   AflIII     AhdI     AleI     AlfI     AloI
     AscI     AseI    AsiSI    AvrII    BamHI     BbeI     BcgI    BfrBI
    BglII    BmgBI     BmtI     BplI    BsaAI    BsiEI    BsiWI     BsmI
    BsmBI   BssHII    BstBI    BstXI   Bsu36I    BtgZI    CspCI     EagI
     EciI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     XbaI     XcmI     XhoI
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