Restriction map for pF1KSDA1562
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AcyI        
AflII       AgeI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AvaI        AviII       
AvrII       BalI        BanII       BbeI        BcgI        
BfrI        BlnI        BsaHI       BseAI       BsiEI       
BsiWI       BsoBI       Bsp1407I    BspDI       BspEI       
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoT22I     FauI        FseI        FspI        
Hin1I       HincII      HindII      HpaI        KasI        
KpnI        KspI        MluI        MluNI       MroI        
MscI        NarI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        SwaI        XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccII        2176  CGCG                 CG^CG
AflIII       2837  ACATGT               A^C[GA][CT]GT
AhdI         1323  GACACCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        1469  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2114  CCTTAGG              CC^T[ACGT]AGG
AseI          388  ATTAAT               AT^TAAT
AsnI          388  ATTAAT               AT^TAAT
Asp700I      1878  GAATATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1323  GACACCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1202  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI         2283  GGTGCC               G^G[CT][GA]CC
BbrPI        2434  CACGTG               CAC^GTG
BciVI        2991  GGATAC               GTATCC(6/5)
BglI         2247  GCCATCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         2911  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2911  GCTCAGC              GC^T[ACGT]AGC
BsaAI        2434  CACGTG               [CT]AC^GT[GA]
BsaBI        1471  GATCCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         2490  GGTCTC               GGTCTC(1/5)
BsaWI        2926  TCCGGT               [AT]^CCGG[AT]
BspHI        1602  TCATGA               T^CATGA
BspLU11I     2837  ACATGT               A^CATGT
BsrFI        2375  GCCGGC               [GA]^CCGG[CT]
BstAPI       2122  GCAATTTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       3142  GGTTACC              G^GT[ACGT]ACC
BstPI        3142  GGTTACC              G^GT[ACGT]ACC
BstUI        2176  CGCG                 CG^CG
Bsu36I       2114  CCTTAGG              CC^T[ACGT]AGG
CelII        2911  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       2375  GCCGGC               [GA]^CCGG[CT]
Eam1105I     1323  GACACCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         2940  GAAGAG               CTCTTC(1/4)
Eco81I       2114  CCTTAGG              CC^T[ACGT]AGG
EcoO65I      3142  GGTTACC              G^GT[ACGT]ACC
HaeII         826  GGCGCT               [GA]GCGC^[CT]
Ksp632I      2940  GAAGAG               CTCTTC(1/4)
MamI         1471  GATCCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I       1848  CAGCTG               C[AC]G^C[GT]G
MvnI         2176  CGCG                 CG^CG
NaeI         2375  GCCGGC               GCC^GGC
NcoI         3263  CCATGG               C^CATGG
NgoMIV       2375  GCCGGC               G^CCGGC
NspI         2837  ACATGT               [GA]CATG^[CT]
PflFI        1202  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2434  CACGTG               CAC^GTG
PmlI         2434  CACGTG               CAC^GTG
PshBI         388  ATTAAT               AT^TAAT
PvuII        1848  CAGCTG               CAG^CTG
RcaI         1602  TCATGA               T^CATGA
SspI         2558  AATATT               AAT^ATT
Tth111I      1202  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         1878  GAATATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AclI         1714  AACGTT               AA^CGTT
             1921  AACGTT              
AspHI        1687  GTGCTC               G[AT]GC[AT]^C
             3310  GTGCTC              
BamHI         813  GGATCC               G^GATCC
             1116  GGATCC              
BbsI         1224  GAAGAC               GAAGAC(2/6)
             2817  GTCTTC              
BbvCI        1990  CCTCAGC              CCTCAGC(-5/-2)
             1309  GCTGAGG             
BclI          329  TGATCA               T^GATCA
             1599  TGATCA              
BcnI          777  CCCGG                CC^[GC]GG
             2252  CCCGG               
BmyI         1687  GTGCTC               G[AGT]GC[ACT]^C
             3310  GTGCTC              
BpmI         3228  CTCCAG               CTGGAG(16/14)
             3103  CTGGAG              
BpuAI        1224  GAAGAC               GAAGAC(2/6)
             2817  GTCTTC              
BsiHKAI      1687  GTGCTC               G[AT]GC[AT]^C
             3310  GTGCTC              
BsmBI        2330  CGTCTC               CGTCTC(1/5)
             3040  CGTCTC              
Bsp1286I     1687  GTGCTC               G[AGT]GC[ACT]^C
             3310  GTGCTC              
BspMI        1864  GCAGGT               ACCTGC(4/8)
             2487  GCAGGT              
DraI          889  TTTAAA               TTT^AAA
             1213  TTTAAA              
EaeI         2213  TGGCCG               [CT]^GGCC[GA]
             2619  TGGCCG              
EcoRI        2729  GAATTC               G^AATTC
             2857  GAATTC              
EcoRV         435  GATATC               GAT^ATC
             1369  GATATC              
EcoT14I      2084  CCTTGG               C^C[AT][AT]GG
             3263  CCATGG              
FbaI          329  TGATCA               T^GATCA
             1599  TGATCA              
HgaI         2908  GACGC                GACGC(5/10)
             3039  GCGTC               
HindIII       169  AAGCTT               A^AGCTT
             1192  AAGCTT              
MfeI          272  CAATTG               C^AATTG
             1814  CAATTG              
MunI          272  CAATTG               C^AATTG
             1814  CAATTG              
NciI          777  CCCGG                CC^[GC]GG
             2252  CCCGG               
NdeI         1229  CATATG               CA^TATG
             1547  CATATG              
PflMI         490  CCAGATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1564  CCATCTAATGG         
PpuMI        2768  GGGTCCT              [GA]G^G[AT]CC[CT]
             3117  GGGACCC             
Psp1406I     1714  AACGTT               AA^CGTT
             1921  AACGTT              
SmlI          977  CTCAAG               C^T[CT][GA]AG
             2899  CTTGAG              
StyI         2084  CCTTGG               C^C[AT][AT]GG
             3263  CCATGG              
Van91I        490  CCAGATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1564  CCATCTAATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BbvI        BfaI        BglII       
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII       
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