Restriction map for pF1KSDA1562
 (Linear) (Six-base) MAPSORT of: KIAA1562.seq  Check: 9742  from: 1  to: 3405



KIAA1562.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AclI AA'CG_TT

Cuts at:      0    1715    1922    3405 
   Size:       1715     207    1483

  Fragments arranged by size: 

                1715    1483     207
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     625     643     837    1204    2035    2051    3405 
   Size:        625      18     194     367     831      16    1354

  Fragments arranged by size: 

                1354     831     625     367     194      18      16
 
AflIII A'CryG_T

Cuts at:      0    2837    3405 
   Size:       2837     568
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1328    3405 
   Size:       1328    2077
 
AleI CACnn'nnGTG

Cuts at:      0    1627    1684    2281    3230    3405 
   Size:       1627      57     597     949     175

  Fragments arranged by size: 

                1627     949     597     175      57
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1474    3405 
   Size:       1474    1931
 
ApoI r'AATT_y

Cuts at:      0      84     216     504     993    1411    2445    2599    2729 
   Size:         84     132     288     489     418    1034     154     130

Cuts at:   2729    2857    3405 
   Size:        128     548

  Fragments arranged by size: 

                1034     548     489     418     288     154     132     130
                 128      84
 
AseI AT'TA_AT

Cuts at:      0     389    3405 
   Size:        389    3016
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1118    1151    3405 
   Size:       1118      33    2254

  Fragments arranged by size: 

                2254    1118      33
 
BamHI G'GATC_C

Cuts at:      0     813    1116    3405 
   Size:        813     303    2289

  Fragments arranged by size: 

                2289     813     303
 
BanI G'GyrC_C

Cuts at:      0    2283    3405 
   Size:       2283    1122
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1231    2810    3405 
   Size:       1231    1579     595

  Fragments arranged by size: 

                1579    1231     595
 
BbvCI CC'TCA_GC

Cuts at:      0    1310    1991    3405 
   Size:       1310     681    1414

  Fragments arranged by size: 

                1414    1310     681
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2985    3405 
   Size:       2985     420
 
BclI T'GATC_A

Cuts at:      0     329    1599    3405 
   Size:        329    1270    1806

  Fragments arranged by size: 

                1806    1270     329
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2253    3405 
   Size:       2253    1152
 
BglII A'GATC_T

Cuts at:      0     415     798    1282    2314    3405 
   Size:        415     383     484    1032    1091

  Fragments arranged by size: 

                1091    1032     484     415     383
 
BlpI GC'TnA_GC

Cuts at:      0    2912    3405 
   Size:       2912     493
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      85     399    1322    2996    3405 
   Size:         85     314     923    1674     409

  Fragments arranged by size: 

                1674     923     409     314      85
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2466    2508    3405 
   Size:       2466      42     897

  Fragments arranged by size: 

                2466     897      42
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3124    3213    3405 
   Size:       3124      89     192

  Fragments arranged by size: 

                3124     192      89
 
Bpu10I CC'TnA_GC

Cuts at:      0    1310    1991    3405 
   Size:       1310     681    1414

  Fragments arranged by size: 

                1414    1310     681
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     962    2920    3405 
   Size:        962    1958     485

  Fragments arranged by size: 

                1958     962     485
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2496    3405 
   Size:       2496     909
 
BsaAI yAC'GTr

Cuts at:      0    2436    3405 
   Size:       2436     969
 
BsaBI GATnn'nnATC

Cuts at:      0    1475    3405 
   Size:       1475    1930
 
BsaWI w'CCGG_w

Cuts at:      0    2926    3405 
   Size:       2926     479
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     610     640    1169    1199    3405 
   Size:        610      30     529      30    2206

  Fragments arranged by size: 

                2206     610     529      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     212    1888    2773    3283    3405 
   Size:        212    1676     885     510     122

  Fragments arranged by size: 

                1676     885     510     212     122
 
BseYI C'CCAG_C

Cuts at:      0    1002    2873    3405 
   Size:       1002    1871     532

  Fragments arranged by size: 

                1871    1002     532
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     467    2150    2736    2784    3256    3405 
   Size:        467    1683     586      48     472     149

  Fragments arranged by size: 

                1683     586     472     467     149      48
 
BsiHKAI G_wGCw'C

Cuts at:      0    1691    3314    3405 
   Size:       1691    1623      91

  Fragments arranged by size: 

                1691    1623      91
 
BsmI GAATG_Cn'

Cuts at:      0      18     464     810    1842    3079    3405 
   Size:         18     446     346    1032    1237     326

  Fragments arranged by size: 

                1237    1032     446     346     326      18
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2336    3046    3405 
   Size:       2336     710     359

  Fragments arranged by size: 

                2336     710     359
 
Bsp1286I G_dGCh'C

Cuts at:      0    1691    3314    3405 
   Size:       1691    1623      91

  Fragments arranged by size: 

                1691    1623      91
 
BspHI T'CATG_A

Cuts at:      0    1602    3405 
   Size:       1602    1803
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1855    2478    3405 
   Size:       1855     623     927

  Fragments arranged by size: 

                1855     927     623
 
BsrDI GCAATG_nn'

Cuts at:      0    1008    1738    2066    3405 
   Size:       1008     730     328    1339

  Fragments arranged by size: 

                1339    1008     730     328
 
BsrFI r'CCGG_y

Cuts at:      0    2375    3405 
   Size:       2375    1030
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2128    3405 
   Size:       2128    1277
 
BstEII G'GTnAC_C

Cuts at:      0    3142    3405 
   Size:       3142     263
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     879    3102    3138    3360    3405 
   Size:        879    2223      36     222      45

  Fragments arranged by size: 

                2223     879     222      45      36
 
BstYI r'GATC_y

Cuts at:      0     415     798     813    1116    1282    1470    2194    2314 
   Size:        415     383      15     303     166     188     724     120

Cuts at:   2314    3272    3405 
   Size:        958     133

  Fragments arranged by size: 

                 958     724     415     383     303     188     166     133
                 120      15
 
Bsu36I CC'TnA_GG

Cuts at:      0    2115    3405 
   Size:       2115    1290
 
BtgI C'CryG_G

Cuts at:      0     809    2622    3263    3405 
   Size:        809    1813     641     142

  Fragments arranged by size: 

                1813     809     641     142
 
BtsI GCAGTG_nn'

Cuts at:      0     647    2138    2803    3033    3084    3405 
   Size:        647    1491     665     230      51     321

  Fragments arranged by size: 

                1491     665     647     321     230      51
 
DraI TTT'AAA

Cuts at:      0     891    1215    3405 
   Size:        891     324    2190

  Fragments arranged by size: 

                2190     891     324
 
EaeI y'GGCC_r

Cuts at:      0    2213    2619    3405 
   Size:       2213     406     786

  Fragments arranged by size: 

                2213     786     406
 
EarI CTCTTCn'nnn_

Cuts at:      0    2935    3405 
   Size:       2935     470
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3385    3405 
   Size:       3385      20
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     625     643     837    1204    2035    2051    3124    3213 
   Size:        625      18     194     367     831      16    1073      89

Cuts at:   3213    3405 
   Size:        192

  Fragments arranged by size: 

                1073     831     625     367     194     192      89      18
                  16
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1732    2769    3118    3405 
   Size:       1732    1037     349     287

  Fragments arranged by size: 

                1732    1037     349     287
 
EcoRI G'AATT_C

Cuts at:      0    2729    2857    3405 
   Size:       2729     128     548

  Fragments arranged by size: 

                2729     548     128
 
EcoRV GAT'ATC

Cuts at:      0     437    1371    3405 
   Size:        437     934    2034

  Fragments arranged by size: 

                2034     934     437
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3138    3405 
   Size:       3138     267
 
HaeII r_GCGC'y

Cuts at:      0     830    3405 
   Size:        830    2575
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     610     642     730     762    1314    1346    1810    1842 
   Size:        610      32      88      32     552      32     464      32

Cuts at:   1842    1885    1917    2179    2211    3405 
   Size:         43      32     262      32    1194

  Fragments arranged by size: 

                1194     610     552     464     262      88      43      32
                  32      32      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     169    1192    3405 
   Size:        169    1023    2213

  Fragments arranged by size: 

                2213    1023     169
 
MfeI C'AATT_G

Cuts at:      0     272    1814    3405 
   Size:        272    1542    1591

  Fragments arranged by size: 

                1591    1542     272
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      99    3119    3405 
   Size:         99    3020     286

  Fragments arranged by size: 

                3020     286      99
 
MslI CAynn'nnrTG

Cuts at:      0    1202    1627    1684    1902    2281    3230    3323    3405 
   Size:       1202     425      57     218     379     949      93      82

  Fragments arranged by size: 

                1202     949     425     379     218      93      82      57
            
 
MspA1I CmG'CkG

Cuts at:      0    1850    3405 
   Size:       1850    1555
 
NaeI GCC'GGC

Cuts at:      0    2377    3405 
   Size:       2377    1028
 
NcoI C'CATG_G

Cuts at:      0    3263    3405 
   Size:       3263     142
 
NdeI CA'TA_TG

Cuts at:      0    1230    1548    3405 
   Size:       1230     318    1857

  Fragments arranged by size: 

                1857    1230     318
 
NgoMIV G'CCGG_C

Cuts at:      0    2375    3405 
   Size:       2375    1030
 
NspI r_CATG'y

Cuts at:      0    2841    3405 
   Size:       2841     564
 
PciI A'CATG_T

Cuts at:      0    2837    3405 
   Size:       2837     568
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     496    1570    3405 
   Size:        496    1074    1835

  Fragments arranged by size: 

                1835    1074     496
 
PfoI T'CCnGG_A

Cuts at:      0    2563    3405 
   Size:       2563     842
 
PmlI CAC'GTG

Cuts at:      0    2436    3405 
   Size:       2436     969
 
PpuMI rG'GwC_Cy

Cuts at:      0    2769    3118    3405 
   Size:       2769     349     287

  Fragments arranged by size: 

                2769     349     287
 
PsiI TTA'TAA

Cuts at:      0    1021    3405 
   Size:       1021    2384
 
PstI C_TGCA'G

Cuts at:      0     356    2209    2922    3405 
   Size:        356    1853     713     483

  Fragments arranged by size: 

                1853     713     483     356
 
PvuII CAG'CTG

Cuts at:      0    1850    3405 
   Size:       1850    1555
 
SanDI GG'GwC_CC

Cuts at:      0    3118    3405 
   Size:       3118     287
 
SfcI C'TryA_G

Cuts at:      0     352    1337    2205    2571    2918    3405 
   Size:        352     985     868     366     347     487

  Fragments arranged by size: 

                 985     868     487     366     352     347
 
SmlI C'TyrA_G

Cuts at:      0     977    2899    3405 
   Size:        977    1922     506

  Fragments arranged by size: 

                1922     977     506
 
SspI AAT'ATT

Cuts at:      0    2560    3405 
   Size:       2560     845
 
StyI C'CwwG_G

Cuts at:      0    2084    3263    3405 
   Size:       2084    1179     142

  Fragments arranged by size: 

                2084    1179     142
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1341    2252    2479    2680    3080    3405 
   Size:       1341     911     227     201     400     325

  Fragments arranged by size: 

                1341     911     400     325     227     201
 
TatI w'GTAC_w

Cuts at:      0      72    2053    3405 
   Size:         72    1981    1352

  Fragments arranged by size: 

                1981    1352      72
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1376    1408    3405 
   Size:       1376      32    1997

  Fragments arranged by size: 

                1997    1376      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1205    3405 
   Size:       1205    2200
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1926    3325    3360    3405 
   Size:       1926    1399      35      45

  Fragments arranged by size: 

                1926    1399      45      35
 
XmnI GAAnn'nnTTC

Cuts at:      0    1882    3405 
   Size:       1882    1523
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     AhdI     AleI    AlwNI     ApoI     AseI
     BaeI    BamHI     BanI     BbsI    BbvCI    BciVI     BclI     BglI
    BglII     BlpI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     DraI     EaeI
     EarI     EciI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI    HaeII
    Hin4I  HindIII     MfeI     MmeI     MslI   MspA1I     NaeI     NcoI
     NdeI   NgoMIV     NspI     PciI    PflMI     PfoI     PmlI    PpuMI
     PsiI     PstI    PvuII    SanDI     SfcI     SmlI     SspI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AlfI
     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII    BanII
     BbeI     BcgI    BfrBI Bme1580I    BmgBI     BmtI    BsaHI    BsiEI
    BsiWI    BspEI    BsrBI    BsrGI   BssHII    BssSI    BstBI  BstZ17I
    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI  EcoICRI    EcoNI
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MluI
     MscI     NarI     NheI     NotI     NruI     NsiI     PacI     PasI
     PmeI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI     XbaI     XhoI     XmaI     ZraI
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