Restriction map for pF1KSDA1547
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvrII       
BamHI       BbrPI       BbvCI       BclI        BfaI        
BfrI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaWI       BseAI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrGI       Bst1107I    BstBI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoRV       EcoT22I     FbaI        FseI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MroI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AatII        1680  GACGTC               GACGT^C
AccI         1465  GTCTAC               GT^[AC][GT]AC
AccII         328  CGCG                 CG^CG
AlwI           83  GGATC                GGATC(4/5)
AviII        1034  TGCGCA               TGC^GCA
BalI         1709  TGGCCA               TGG^CCA
BbsI          159  GAAGAC               GAAGAC(2/6)
BciVI           3  GTATCC               GTATCC(6/5)
BpuAI         159  GAAGAC               GAAGAC(2/6)
BsaI          448  GGTCTC               GGTCTC(1/5)
BseRI         688  GAGGAG               GAGGAG(10/8)
BsiEI        1198  CGATCG               CG[GA][CT]^CG
BsrBI        1569  CCGCTC               CCGCTC(-3/-3)
BsrDI        1761  CATTGC               GCAATG(2/0)
BssHII        327  GCGCGC               G^CGCGC
BstEII        339  GGTCACC              G^GT[ACGT]ACC
BstPI         339  GGTCACC              G^GT[ACGT]ACC
BstUI         328  CGCG                 CG^CG
BstXI        1812  CCACACAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI           52  TTTAAA               TTT^AAA
DrdI         1723  GACGCCAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1416  CCACGG               C^C[GA][CT]GG
EcoNI        1902  CCTGCGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       339  GGTCACC              G^GT[ACGT]ACC
EcoRI          94  GAATTC               G^AATTC
FspI         1034  TGCGCA               TGC^GCA
MluNI        1709  TGGCCA               TGG^CCA
MscI         1709  TGGCCA               TGG^CCA
MseI           53  TTAA                 T^TAA
MvnI          328  CGCG                 CG^CG
PleI           70  GAGTC                GAGTC(4/5)
PvuI         1198  CGATCG               CGAT^CG
StuI         1260  AGGCCT               AGG^CCT
SwaI           51  ATTTAAAT             ATTT^AAAT
Tru9I          53  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcsI           55  AAATTC               [GA]^AATT[CT]
               94  GAATTC              
ApoI           55  AAATTC               [GA]^AATT[CT]
               94  GAATTC              
BcgI         1598  GCACGCTCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2099  GCAAGTGGTTCG        
BglI          862  GCCTCGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1508  GCCAGCCAGGC         
BglII        1493  AGATCT               A^GATCT
             1946  AGATCT              
BsmAI         249  GAGAC                GTCTC(1/5)
              449  GTCTC               
BsrFI        1020  GCCGGC               [GA]^CCGG[CT]
             1132  GCCGGC              
BssSI        1455  CACGAG               CACGAG(-5/-1)
             2059  CACGAG              
BstYI        1493  AGATCT               [GA]^GATC[CT]
             1946  AGATCT              
Cfr10I       1020  GCCGGC               [GA]^CCGG[CT]
             1132  GCCGGC              
EaeI         1194  TGGCCG               [CT]^GGCC[GA]
             1709  TGGCCA              
EarI          873  CTCTTC               CTCTTC(1/4)
              246  GAAGAG              
EcoT14I       909  CCTTGG               C^C[AT][AT]GG
              964  CCAAGG              
Ksp632I       873  CTCTTC               CTCTTC(1/4)
              246  GAAGAG              
MflI         1493  AGATCT               [GA]^GATC[CT]
             1946  AGATCT              
NaeI         1020  GCCGGC               GCC^GGC
             1132  GCCGGC              
NgoMIV       1020  GCCGGC               G^CCGGC
             1132  GCCGGC              
PflMI        1785  CCAGACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1856  CCAAACTGTGG         
PshAI         907  GACCTTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1788  GACCCTGGTC          
PstI         1178  CTGCAG               CTGCA^G
             1746  CTGCAG              
SmaI           37  CCCGGG               CCC^GGG
              779  CCCGGG              
StyI          909  CCTTGG               C^C[AT][AT]GG
              964  CCAAGG              
TaqI          566  TCGA                 T^CGA
             1015  TCGA                
TthHB8I       566  TCGA                 T^CGA
             1015  TCGA                
Van91I       1785  CCAGACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1856  CCAAACTGTGG         
XcmI         1529  CCATCTCCCAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1634  CCATCTGGGACCTGG     
XhoII        1493  AGATCT               [GA]^GATC[CT]
             1946  AGATCT              
XmaCI          37  CCCGGG               C^CCGGG
              779  CCCGGG              
XmaI           37  CCCGGG               C^CCGGG
              779  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwNI       
AspHI       AvaI        AvaII       BanI        BanII       
BbeI        BbvI        BcnI        BmyI        BpmI        
BsaHI       BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        MaeII       MaeIII      
MboI        MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MwoI        NarI        NciI        
NdeII       NlaIII      NlaIV       PpuMI       PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmlI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       
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