Restriction map for pF1KSDA1547
 (Linear) (Six-base) MAPSORT of: KIAA1547.seq  Check: 2123  from: 1  to: 2280



KIAA1547.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     297    2063    2280 
   Size:        297    1766     217

  Fragments arranged by size: 

                1766     297     217
 
AatII G_ACGT'C

Cuts at:      0    1684    2280 
   Size:       1684     596
 
AccI GT'mk_AC

Cuts at:      0    1466    2280 
   Size:       1466     814
 
AlwNI CAG_nnn'CTG

Cuts at:      0     105    1186    1791    2242    2280 
   Size:        105    1081     605     451      38

  Fragments arranged by size: 

                1081     605     451     105      38
 
ApoI r'AATT_y

Cuts at:      0      55      94    2280 
   Size:         55      39    2186

  Fragments arranged by size: 

                2186      55      39
 
AvaI C'yCGr_G

Cuts at:      0      37     779    1383    2280 
   Size:         37     742     604     897

  Fragments arranged by size: 

                 897     742     604      37
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     702     735    2280 
   Size:        702      33    1545

  Fragments arranged by size: 

                1545     702      33
 
BanI G'GyrC_C

Cuts at:      0     508     988    1068    1123    1852    2280 
   Size:        508     480      80      55     729     428

  Fragments arranged by size: 

                 729     508     480     428      80      55
 
BanII G_rGCy'C

Cuts at:      0     649     944    1010    1528    2280 
   Size:        649     295      66     518     752

  Fragments arranged by size: 

                 752     649     518     295      66
 
BbeI G_GCGC'C

Cuts at:      0     512    1072    1127    2280 
   Size:        512     560      55    1153

  Fragments arranged by size: 

                1153     560     512      55
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     166    2280 
   Size:        166    2114
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1587    1621    2088    2122    2280 
   Size:       1587      34     467      34     158

  Fragments arranged by size: 

                1587     467     158      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      14    2280 
   Size:         14    2266
 
BglI GCCn_nnn'nGGC

Cuts at:      0     868    1514    2280 
   Size:        868     646     766

  Fragments arranged by size: 

                 868     766     646
 
BglII A'GATC_T

Cuts at:      0    1493    1946    2280 
   Size:       1493     453     334

  Fragments arranged by size: 

                1493     453     334
 
Bme1580I G_kGCm'C

Cuts at:      0    1349    2280 
   Size:       1349     931
 
BmgBI CAC'GTC

Cuts at:      0    1464    2280 
   Size:       1464     816
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     466     514    1982    2126    2280 
   Size:        466      48    1468     144     154

  Fragments arranged by size: 

                1468     466     154     144      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     367     436     443     968    1310    2163    2280 
   Size:        367      69       7     525     342     853     117

  Fragments arranged by size: 

                 853     525     367     342     117      69       7
 
Bpu10I CC'TnA_GC

Cuts at:      0    1009    2280 
   Size:       1009    1271
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     723     928    1254    2065    2217    2280 
   Size:        723     205     326     811     152      63

  Fragments arranged by size: 

                 811     723     326     205     152      63
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     454    2280 
   Size:        454    1826
 
BsaHI Gr'CG_yC

Cuts at:      0     509     974    1069    1124    1361    1681    1724    2149 
   Size:        509     465      95      55     237     320      43     425

Cuts at:   2149    2280 
   Size:        131

  Fragments arranged by size: 

                 509     465     425     320     237     131      95      55
                  43
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     913     943    1414    1444    1555    1585    2280 
   Size:        913      30     471      30     111      30     695

  Fragments arranged by size: 

                 913     695     471     111      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     703    2280 
   Size:        703    1577
 
BseYI C'CCAG_C

Cuts at:      0    1535    1956    2280 
   Size:       1535     421     324

  Fragments arranged by size: 

                1535     421     324
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1762    2005    2035    2041    2280 
   Size:       1762     243      30       6     239

  Fragments arranged by size: 

                1762     243     239      30       6
 
BsiEI CG_ry'CG

Cuts at:      0    1201    2280 
   Size:       1201    1079
 
BsiHKAI G_wGCw'C

Cuts at:      0     299    2075    2116    2280 
   Size:        299    1776      41     164

  Fragments arranged by size: 

                1776     299     164      41
 
Bsp1286I G_dGCh'C

Cuts at:      0     299     649     944    1010    1349    1528    2075    2116 
   Size:        299     350     295      66     339     179     547      41

Cuts at:   2116    2280 
   Size:        164

  Fragments arranged by size: 

                 547     350     339     299     295     179     164      66
                  41
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     297     327    1784    1910    2063    2280 
   Size:        297      30    1457     126     153     217

  Fragments arranged by size: 

                1457     297     217     153     126      30
 
BsrBI CCG'CTC

Cuts at:      0    1571    2280 
   Size:       1571     709
 
BsrDI GCAATG_nn'

Cuts at:      0    1760    2280 
   Size:       1760     520
 
BsrFI r'CCGG_y

Cuts at:      0    1020    1132    2280 
   Size:       1020     112    1148

  Fragments arranged by size: 

                1148    1020     112
 
BssHII G'CGCG_C

Cuts at:      0     327    2280 
   Size:        327    1953
 
BssSI C'ACGA_G

Cuts at:      0    1455    2059    2280 
   Size:       1455     604     221

  Fragments arranged by size: 

                1455     604     221
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1186    1344    1484    2280 
   Size:       1186     158     140     796

  Fragments arranged by size: 

                1186     796     158     140
 
BstEII G'GTnAC_C

Cuts at:      0     339    2280 
   Size:        339    1941
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1819    2280 
   Size:       1819     461
 
BstYI r'GATC_y

Cuts at:      0    1493    1946    2280 
   Size:       1493     453     334

  Fragments arranged by size: 

                1493     453     334
 
BtgI C'CryG_G

Cuts at:      0    1416    2280 
   Size:       1416     864
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1766    2280 
   Size:       1766     514
 
BtsI GCAGTG_nn'

Cuts at:      0     326    2280 
   Size:        326    1954
 
DraI TTT'AAA

Cuts at:      0      54    2280 
   Size:         54    2226
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1729    2280 
   Size:       1729     551
 
EaeI y'GGCC_r

Cuts at:      0    1194    1709    2280 
   Size:       1194     515     571

  Fragments arranged by size: 

                1194     571     515
 
EarI CTCTTCn'nnn_

Cuts at:      0     241     879    2280 
   Size:        241     638    1401

  Fragments arranged by size: 

                1401     638     241
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     700    2280 
   Size:        700    1580
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     367     436     443     968    1310    2163    2280 
   Size:        367      69       7     525     342     853     117

  Fragments arranged by size: 

                 853     525     367     342     117      69       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1906    2280 
   Size:       1906     374
 
EcoO109I rG'GnC_Cy

Cuts at:      0     765    1372    1641    1773    1872    1899    2280 
   Size:        765     607     269     132      99      27     381

  Fragments arranged by size: 

                 765     607     381     269     132      99      27
 
EcoRI G'AATT_C

Cuts at:      0      94    2280 
   Size:         94    2186
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     717    2146    2280 
   Size:        717    1429     134

  Fragments arranged by size: 

                1429     717     134
 
FspI TGC'GCA

Cuts at:      0    1036    2280 
   Size:       1036    1244
 
HaeII r_GCGC'y

Cuts at:      0     177     512    1072    1127    1166    2280 
   Size:        177     335     560      55      39    1114

  Fragments arranged by size: 

                1114     560     335     177      55      39
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     568     600     723     755     913     945    1633    1665 
   Size:        568      32     123      32     158      32     688      32

Cuts at:   1665    1666    1698    2280 
   Size:          1      32     582

  Fragments arranged by size: 

                 688     582     568     158     123      32      32      32
                  32      32       1
 
KasI G'GCGC_C

Cuts at:      0     508    1068    1123    2280 
   Size:        508     560      55    1157

  Fragments arranged by size: 

                1157     560     508      55
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     974    2280 
   Size:        974    1306
 
MscI TGG'CCA

Cuts at:      0    1711    2280 
   Size:       1711     569
 
MslI CAynn'nnrTG

Cuts at:      0     220    1319    1817    2280 
   Size:        220    1099     498     463

  Fragments arranged by size: 

                1099     498     463     220
 
MspA1I CmG'CkG

Cuts at:      0    1174    1186    1539    1829    2049    2215    2280 
   Size:       1174      12     353     290     220     166      65

  Fragments arranged by size: 

                1174     353     290     220     166      65      12
 
NaeI GCC'GGC

Cuts at:      0    1022    1134    2280 
   Size:       1022     112    1146

  Fragments arranged by size: 

                1146    1022     112
 
NarI GG'CG_CC

Cuts at:      0     509    1069    1124    2280 
   Size:        509     560      55    1156

  Fragments arranged by size: 

                1156     560     509      55
 
NgoMIV G'CCGG_C

Cuts at:      0    1020    1132    2280 
   Size:       1020     112    1148

  Fragments arranged by size: 

                1148    1020     112
 
PasI CC'CwG_GG

Cuts at:      0     513     937    1003    2280 
   Size:        513     424      66    1277

  Fragments arranged by size: 

                1277     513     424      66
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1791    1862    2280 
   Size:       1791      71     418

  Fragments arranged by size: 

                1791     418      71
 
PfoI T'CCnGG_A

Cuts at:      0     459    1287    2280 
   Size:        459     828     993

  Fragments arranged by size: 

                 993     828     459
 
PpuMI rG'GwC_Cy

Cuts at:      0     765    1641    1773    1899    2280 
   Size:        765     876     132     126     381

  Fragments arranged by size: 

                 876     765     381     132     126
 
PshAI GACnn'nnGTC

Cuts at:      0     911    1792    2280 
   Size:        911     881     488

  Fragments arranged by size: 

                 911     881     488
 
PstI C_TGCA'G

Cuts at:      0    1182    1750    2280 
   Size:       1182     568     530

  Fragments arranged by size: 

                1182     568     530
 
PvuI CG_AT'CG

Cuts at:      0    1201    2280 
   Size:       1201    1079
 
PvuII CAG'CTG

Cuts at:      0    1539    1829    2049    2280 
   Size:       1539     290     220     231

  Fragments arranged by size: 

                1539     290     231     220
 
SfcI C'TryA_G

Cuts at:      0     657    1178    1746    1921    2280 
   Size:        657     521     568     175     359

  Fragments arranged by size: 

                 657     568     521     359     175
 
SfoI GGC'GCC

Cuts at:      0     510    1070    1125    2280 
   Size:        510     560      55    1155

  Fragments arranged by size: 

                1155     560     510      55
 
SmaI CCC'GGG

Cuts at:      0      39     781    2280 
   Size:         39     742    1499

  Fragments arranged by size: 

                1499     742      39
 
SmlI C'TyrA_G

Cuts at:      0     702     943    1269    2080    2196    2280 
   Size:        702     241     326     811     116      84

  Fragments arranged by size: 

                 811     702     326     241     116      84
 
StuI AGG'CCT

Cuts at:      0    1262    2280 
   Size:       1262    1018
 
StyI C'CwwG_G

Cuts at:      0     909     964    2280 
   Size:        909      55    1316

  Fragments arranged by size: 

                1316     909      55
 
SwaI ATTT'AAAT

Cuts at:      0      54    2280 
   Size:         54    2226
 
TatI w'GTAC_w

Cuts at:      0     188    2280 
   Size:        188    2092
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1414    1446    2280 
   Size:       1414      32     834

  Fragments arranged by size: 

                1414     834      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1536    1641    2280 
   Size:       1536     105     639

  Fragments arranged by size: 

                1536     639     105
 
XmaI C'CCGG_G

Cuts at:      0      37     779    2280 
   Size:         37     742    1501

  Fragments arranged by size: 

                1501     742      37
 
ZraI GAC'GTC

Cuts at:      0    1682    2280 
   Size:       1682     598
 

 Enzymes that do cut:

     AarI    AatII     AccI    AlwNI     ApoI     AvaI     BaeI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BglI    BglII Bme1580I
    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     PasI    PflMI     PfoI    PpuMI    PshAI     PstI     PvuI    PvuII
     SfcI     SfoI     SmaI     SmlI     StuI     StyI     SwaI     TatI
     TstI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII    BamHI    BbvCI     BclI    BfrBI     BlpI     BmtI     BplI
    BsaAI    BsaBI    BsaWI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrGI    BstBI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII     EagI
  EcoICRI    EcoRV     FseI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI     PmeI     PmlI     PpiI     PsiI   PspOMI
    PspXI     PsrI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI    TaqII  Tth111I     XbaI     XhoI     XmnI
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