Restriction map for pF1KSDA1542
##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

AclI        AcsI        AflII       AgeI        AhdI        
Aor51HI     ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AvrII       BclI        BfrI        
BlnI        BsaBI       Bsp1407I    BspDI       BspHI       
BsrGI       BssHII      Bst1107I    BstBI       BstZ17I     
ClaI        DraI        DraIII      Eam1105I    Eco47III    
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HindIII     HpaI        MamI        MfeI        MluI        
MunI        NdeI        NotI        NruI        NsiI        
NspV        PacI        PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        ScaI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
SspBI       SwaI        XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AatII        3261  GACGTC               GACGT^C
Acc65I        620  GGTACC               G^GTACC
Alw44I       2227  GTGCAC               G^TGCAC
AocI         4150  CCTGAGG              CC^T[ACGT]AGG
ApaI         3237  GGGCCC               GGGCC^C
ApaLI        2227  GTGCAC               G^TGCAC
Asp718I       620  GGTACC               G^GTACC
AspI         3558  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1320  GGATCC               G^GATCC
BbrPI        4372  CACGTG               CAC^GTG
BciVI        2042  GGATAC               GTATCC(6/5)
BsiWI        4865  CGTACG               C^GTACG
BspLU11I     3678  ACATGT               A^CATGT
BsrDI         245  CATTGC               GCAATG(2/0)
BssSI        3427  CACGAG               CACGAG(-5/-1)
Bsu36I       4150  CCTGAGG              CC^T[ACGT]AGG
CpoI         2688  CGGACCG              CG^G[AT]CCG
EagI         3701  CGGCCG               C^GGCCG
EclXI        3701  CGGCCG               C^GGCCG
Eco52I       3701  CGGCCG               C^GGCCG
Eco81I       4150  CCTGAGG              CC^T[ACGT]AGG
HincII       2313  GTCGAC               GT[CT]^[GA]AC
HindII       2313  GTCGAC               GT[CT]^[GA]AC
KpnI          620  GGTACC               GGTAC^C
NaeI         3152  GCCGGC               GCC^GGC
NcoI         2265  CCATGG               C^CATGG
NgoMIV       3152  GCCGGC               G^CCGGC
NheI         4647  GCTAGC               G^CTAGC
PflFI        3558  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        4372  CACGTG               CAC^GTG
PmlI         4372  CACGTG               CAC^GTG
RsrII        2688  CGGACCG              CG^G[AT]CCG
SalI         2313  GTCGAC               G^TCGAC
SspI         2338  AATATT               AAT^ATT
StuI         1201  AGGCCT               AGG^CCT
Tth111I      3558  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI         2313  GTCGAC               GT^[AC][GT]AC
             2485  GTCTAC              
BbvCI        1590  CCTCAGC              CCTCAGC(-5/-2)
             4063  GCTGAGG             
BcgI         2465  CGAAGCAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3676  GCACATGTCTCG        
BfaI         4525  CTAG                 C^TAG
             4648  CTAG                
BlpI         1088  GCTCAGC              GC^T[ACGT]AGC
             1746  GCTCAGC             
Bpu1102I     1088  GCTCAGC              GC^T[ACGT]AGC
             1746  GCTCAGC             
BsaAI        4372  CACGTG               [CT]AC^GT[GA]
             4867  TACGTG              
BsmBI        1434  CGTCTC               CGTCTC(1/5)
             1380  GAGACG              
CelII        1088  GCTCAGC              GC^T[ACGT]AGC
             1746  GCTCAGC             
DrdI          397  GACTGCATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3261  GACGTCCCGGTC        
KspI         1469  CCGCGG               CCGC^GG
             1784  CCGCGG              
MaeI         4525  CTAG                 C^TAG
             4648  CTAG                
MseI          455  TTAA                 T^TAA
              496  TTAA                
MslI          148  CATGGAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              963  CACTGCCGTG          
PaeR7I        794  CTCGAG               C^TCGAG
             3087  CTCGAG              
SacII        1469  CCGCGG               CCGC^GG
             1784  CCGCGG              
SapI         1339  GAAGAGC              GCTCTTC(1/4)
             3293  GCTCTTC             
SbfI         3717  CCTGCAGG             CCTGCA^GG
             4218  CCTGCAGG            
SexAI        3652  ACCAGGT              A^CC[AT]GGT
             4853  ACCTGGT             
SfiI         3238  GGCCCCTGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             3702  GGCCGACAAGGCC       
Sse8387I     3717  CCTGCAGG             CCTGCA^GG
             4218  CCTGCAGG            
Tru9I         455  TTAA                 T^TAA
              496  TTAA                
XhoI          794  CTCGAG               C^TCGAG
             3087  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  135 enzymes) #
#########################################

AccII       AccIII      AciI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       AspHI       
AvaI        AvaII       AviII       BalI        BanI        
BanII       BbeI        BbsI        BbvI        BcnI        
BglI        BglII       BmyI        BpmI        BpuAI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspEI       BspMI       BsrBI       BsrFI       
BsrI        BssKI       BstAPI      BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MroI        
MscI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmaI        SmlI        StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmaCI       XmaI        
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