Restriction map for pF1KSDA1542
 (Linear) (Six-base) MAPSORT of: KIAA1542.seq  Check: 1883  from: 1  to: 4947



KIAA1542.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      73    4947 
   Size:         73    4874
 
AatII G_ACGT'C

Cuts at:      0    3265    4947 
   Size:       3265    1682
 
AccI GT'mk_AC

Cuts at:      0    2314    2486    4947 
   Size:       2314     172    2461

  Fragments arranged by size: 

                2461    2314     172
 
Acc65I G'GTAC_C

Cuts at:      0     620    4947 
   Size:        620    4327
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     248    1854    2020    3934    4492    4947 
   Size:        248    1606     166    1914     558     455

  Fragments arranged by size: 

                1914    1606     558     455     248     166
 
AflIII A'CryG_T

Cuts at:      0    2393    3678    3804    4947 
   Size:       2393    1285     126    1143

  Fragments arranged by size: 

                2393    1285    1143     126
 
AleI CACnn'nnGTG

Cuts at:      0     967    4947 
   Size:        967    3980
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     336    1999    2224    4721    4813    4947 
   Size:        336    1663     225    2497      92     134

  Fragments arranged by size: 

                2497    1663     336     225     134      92
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     658     690     856     888    4375    4376    4407    4408 
   Size:        658      32     166      32    3487       1      31       1

Cuts at:   4408    4947 
   Size:        539

  Fragments arranged by size: 

                3487     658     539     166      32      32      31       1
                   1
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1217    1507    1822    1889    2126    2728    2781    4551 
   Size:       1217     290     315      67     237     602      53    1770

Cuts at:   4551    4947 
   Size:        396

  Fragments arranged by size: 

                1770    1217     602     396     315     290     237      67
                  53
 
ApaI G_GGCC'C

Cuts at:      0    3241    4947 
   Size:       3241    1706
 
ApaLI G'TGCA_C

Cuts at:      0    2227    4947 
   Size:       2227    2720
 
AvaI C'yCGr_G

Cuts at:      0     794     809     910    1682    2213    2221    2539    3087 
   Size:        794      15     101     772     531       8     318     548

Cuts at:   3087    3353    3464    4262    4292    4387    4947 
   Size:        266     111     798      30      95     560

  Fragments arranged by size: 

                 798     794     772     560     548     531     318     266
                 111     101      95      30      15       8
 
BamHI G'GATC_C

Cuts at:      0    1320    4947 
   Size:       1320    3627
 
BanI G'GyrC_C

Cuts at:      0     620     657     768     905    1457    1473    1791    1900 
   Size:        620      37     111     137     552      16     318     109

Cuts at:   1900    1983    2155    2208    4018    4068    4089    4266    4947 
   Size:         83     172      53    1810      50      21     177     681

  Fragments arranged by size: 

                1810     681     620     552     318     177     172     137
                 111     109      83      53      50      37      21      16
            
 
BanII G_rGCy'C

Cuts at:      0      40     477    1090    1250    1389    1705    1748    2222 
   Size:         40     437     613     160     139     316      43     474

Cuts at:   2222    2675    2765    2819    2960    3088    3241    3286    3862 
   Size:        453      90      54     141     128     153      45     576

Cuts at:   3862    4194    4260    4947 
   Size:        332      66     687

  Fragments arranged by size: 

                 687     613     576     474     453     437     332     316
                 160     153     141     139     128      90      66      54
                  45      43      40
 
BbeI G_GCGC'C

Cuts at:      0     909    1461    1795    4093    4947 
   Size:        909     552     334    2298     854

  Fragments arranged by size: 

                2298     909     854     552     334
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     176     200     209     236     908    1024    1032    2467 
   Size:        176      24       9      27     672     116       8    1435

Cuts at:   2467    3647    3992    4112    4636    4947 
   Size:       1180     345     120     524     311

  Fragments arranged by size: 

                1435    1180     672     524     345     311     176     120
                 116      27      24       9       8
 
BbvCI CC'TCA_GC

Cuts at:      0    1591    4064    4947 
   Size:       1591    2473     883

  Fragments arranged by size: 

                2473    1591     883
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2454    2488    3665    3699    4947 
   Size:       2454      34    1177      34    1248

  Fragments arranged by size: 

                2454    1248    1177      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2036    4947 
   Size:       2036    2911
 
BglI GCCn_nnn'nGGC

Cuts at:      0     940    1603    1800    1825    2737    3245    3709    3940 
   Size:        940     663     197      25     912     508     464     231

Cuts at:   3940    4264    4947 
   Size:        324     683

  Fragments arranged by size: 

                 940     912     683     663     508     464     324     231
                 197      25
 
BglII A'GATC_T

Cuts at:      0     208    1099    1943    4805    4947 
   Size:        208     891     844    2862     142

  Fragments arranged by size: 

                2862     891     844     208     142
 
BlpI GC'TnA_GC

Cuts at:      0    1089    1747    4947 
   Size:       1089     658    3200

  Fragments arranged by size: 

                3200    1089     658
 
Bme1580I G_kGCm'C

Cuts at:      0     381     773    2158    2213    2231    3241    3516    4021 
   Size:        381     392    1385      55      18    1010     275     505

Cuts at:   4021    4269    4565    4947 
   Size:        248     296     382

  Fragments arranged by size: 

                1385    1010     505     392     382     381     296     275
                 248      55      18
 
BmgBI CAC'GTC

Cuts at:      0    2925    3807    4947 
   Size:       2925     882    1140

  Fragments arranged by size: 

                2925    1140     882
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     502    2114    2293    4067    4361    4552    4947 
   Size:        502    1612     179    1774     294     191     395

  Fragments arranged by size: 

                1774    1612     502     395     294     191     179
 
BmtI G_CTAG'C

Cuts at:      0    4651    4947 
   Size:       4651     296
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     762    1403    1625    2454    2757    4947 
   Size:        762     641     222     829     303    2190

  Fragments arranged by size: 

                2190     829     762     641     303     222
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     634    1595    2358    2502    3147    3159    3406 
   Size:        271     363     961     763     144     645      12     247

Cuts at:   3406    3757    4947 
   Size:        351    1190

  Fragments arranged by size: 

                1190     961     763     645     363     351     271     247
                 144      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     958    1591    2959    4039    4064    4947 
   Size:        958     633    1368    1080      25     883

  Fragments arranged by size: 

                1368    1080     958     883     633      25
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1932    3807    4440    4947 
   Size:       1932    1875     633     507

  Fragments arranged by size: 

                1932    1875     633     507
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     341     750    3461    4947 
   Size:        341     409    2711    1486

  Fragments arranged by size: 

                2711    1486     409     341
 
BsaAI yAC'GTr

Cuts at:      0    4374    4869    4947 
   Size:       4374     495      78

  Fragments arranged by size: 

                4374     495      78
 
BsaHI Gr'CG_yC

Cuts at:      0     181     364     906     988    1458    1792    2839    3262 
   Size:        181     183     542      82     470     334    1047     423

Cuts at:   3262    3830    4090    4947 
   Size:        568     260     857

  Fragments arranged by size: 

                1047     857     568     542     470     423     334     260
                 183     181      82
 
BsaWI w'CCGG_w

Cuts at:      0     860     866    1063    2330    3470    4380    4947 
   Size:        860       6     197    1267    1140     910     567

  Fragments arranged by size: 

                1267    1140     910     860     567     197       6
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     564     594     856     886    4947 
   Size:        564      30     262      30    4061

  Fragments arranged by size: 

                4061     564     262      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     202     547     550     553     751     754    1507    1510 
   Size:        202     345       3       3     198       3     753       3

Cuts at:   1510    2773    2842    2881    3130    4168    4639    4687    4729 
   Size:       1263      69      39     249    1038     471      48      42

Cuts at:   4729    4783    4933    4947 
   Size:         54     150      14

  Fragments arranged by size: 

                1263    1038     753     471     345     249     202     198
                 150      69      54      48      42      39      14       3
                   3       3       3
 
BseYI C'CCAG_C

Cuts at:      0    1188    1515    1551    2250    2257    2914    3606    3930 
   Size:       1188     327      36     699       7     657     692     324

Cuts at:   3930    4448    4578    4947 
   Size:        518     130     369

  Fragments arranged by size: 

                1188     699     692     657     518     369     327     324
                 130      36       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2638    2741    4675    4819    4947 
   Size:       2638     103    1934     144     128

  Fragments arranged by size: 

                2638    1934     144     128     103
 
BsiEI CG_ry'CG

Cuts at:      0     579    3271    3277    3704    4947 
   Size:        579    2692       6     427    1243

  Fragments arranged by size: 

                2692    1243     579     427       6
 
BsiHKAI G_wGCw'C

Cuts at:      0     477     964    1090    1689    1741    2231    2960    3041 
   Size:        477     487     126     599      52     490     729      81

Cuts at:   3041    3088    3230    3308    3370    3509    3524    3862    4947 
   Size:         47     142      78      62     139      15     338    1085

  Fragments arranged by size: 

                1085     729     599     490     487     477     338     142
                 139     126      81      78      62      52      47      15
            
 
BsiWI C'GTAC_G

Cuts at:      0    4865    4947 
   Size:       4865      82
 
BsmI GAATG_Cn'

Cuts at:      0     340     426     637    4947 
   Size:        340      86     211    4310

  Fragments arranged by size: 

                4310     340     211      86
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1374    1440    4947 
   Size:       1374      66    3507

  Fragments arranged by size: 

                3507    1374      66
 
Bsp1286I G_dGCh'C

Cuts at:      0      40     381     477     773     964    1090    1250    1389 
   Size:         40     341      96     296     191     126     160     139

Cuts at:   1389    1689    1705    1741    1748    2158    2213    2222    2231 
   Size:        300      16      36       7     410      55       9       9

Cuts at:   2231    2675    2765    2819    2960    3041    3088    3230    3241 
   Size:        444      90      54     141      81      47     142      11

Cuts at:   3241    3286    3308    3370    3509    3516    3524    3862    4021 
   Size:         45      22      62     139       7       8     338     159

Cuts at:   4021    4194    4260    4269    4565    4947 
   Size:        173      66       9     296     382

  Fragments arranged by size: 

                 444     410     382     341     338     300     296     296
                 191     173     160     159     142     141     139     139
                 126      96      90      81      66      62      55      54
                  47      45      40      36      22      16      11       9
                   9       9       8       7       7
 
BspEI T'CCGG_A

Cuts at:      0    1063    2330    4380    4947 
   Size:       1063    1267    2050     567

  Fragments arranged by size: 

                2050    1267    1063     567
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      73     790    1520    1751    4226    4947 
   Size:         73     717     730     231    2475     721

  Fragments arranged by size: 

                2475     730     721     717     231      73
 
BsrBI CCG'CTC

Cuts at:      0    1270    2871    2981    3173    3432    4947 
   Size:       1270    1601     110     192     259    1515

  Fragments arranged by size: 

                1601    1515    1270     259     192     110
 
BsrDI GCAATG_nn'

Cuts at:      0     244    4947 
   Size:        244    4703
 
BsrFI r'CCGG_y

Cuts at:      0     660    2506    3152    4947 
   Size:        660    1846     646    1795

  Fragments arranged by size: 

                1846    1795     660     646
 
BssSI C'ACGA_G

Cuts at:      0    3427    4947 
   Size:       3427    1520
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1917    2625    2728    4947 
   Size:       1917     708     103    2219

  Fragments arranged by size: 

                2219    1917     708     103
 
BstEII G'GTnAC_C

Cuts at:      0    2877    4206    4758    4947 
   Size:       2877    1329     552     189

  Fragments arranged by size: 

                2877    1329     552     189
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2819    3384    4194    4947 
   Size:       2819     565     810     753

  Fragments arranged by size: 

                2819     810     753     565
 
BstYI r'GATC_y

Cuts at:      0     208     503     869    1099    1305    1320    1943    2114 
   Size:        208     295     366     230     206      15     623     171

Cuts at:   2114    4805    4947 
   Size:       2691     142

  Fragments arranged by size: 

                2691     623     366     295     230     208     206     171
                 142      15
 
Bsu36I CC'TnA_GG

Cuts at:      0    4151    4947 
   Size:       4151     796
 
BtgI C'CryG_G

Cuts at:      0     356     875    1421    1439    1469    1784    2097    2265 
   Size:        356     519     546      18      30     315     313     168

Cuts at:   2265    3791    3944    4490    4947 
   Size:       1526     153     546     457

  Fragments arranged by size: 

                1526     546     546     519     457     356     315     313
                 168     153      30      18
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     626    4947 
   Size:        626    4321
 
BtsI GCAGTG_nn'

Cuts at:      0     120     962    1341    1566    1687    1983    2729    4604 
   Size:        120     842     379     225     121     296     746    1875

Cuts at:   4604    4947 
   Size:        343

  Fragments arranged by size: 

                1875     842     746     379     343     296     225     121
                 120
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     403    3267    4947 
   Size:        403    2864    1680

  Fragments arranged by size: 

                2864    1680     403
 
EaeI y'GGCC_r

Cuts at:      0     878    1424    3433    3550    3701    3941    3947    4727 
   Size:        878     546    2009     117     151     240       6     780

Cuts at:   4727    4847    4947 
   Size:        120     100

  Fragments arranged by size: 

                2009     878     780     546     240     151     120     117
                 100       6
 
EagI C'GGCC_G

Cuts at:      0    3701    4947 
   Size:       3701    1246
 
EarI CTCTTCn'nnn_

Cuts at:      0    1006    1123    1128    1334    2452    3016    3300    3894 
   Size:       1006     117       5     206    1118     564     284     594

Cuts at:   3894    4744    4947 
   Size:        850     203

  Fragments arranged by size: 

                1118    1006     850     594     564     284     206     203
                 117       5
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2688    4947 
   Size:       2688    2259
 
EcoICRI GAG'CTC

Cuts at:      0     475    1088    2958    3086    3860    4947 
   Size:        475     613    1870     128     774    1087

  Fragments arranged by size: 

                1870    1087     774     613     475     128
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     248     271     634    1595    1854    2020    2358    2502 
   Size:        248      23     363     961     259     166     338     144

Cuts at:   2502    3147    3159    3406    3757    3934    4492    4947 
   Size:        645      12     247     351     177     558     455

  Fragments arranged by size: 

                 961     645     558     455     363     351     338     259
                 248     247     177     166     144      23      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     797    3619    4012    4789    4858    4947 
   Size:        797    2822     393     777      69      89

  Fragments arranged by size: 

                2822     797     777     393      89      69
 
EcoO109I rG'GnC_Cy

Cuts at:      0     543     724     806    1763    1990    2738    2778    2963 
   Size:        543     181      82     957     227     748      40     185

Cuts at:   2963    3237    3238    3485    3711    4947 
   Size:        274       1     247     226    1236

  Fragments arranged by size: 

                1236     957     748     543     274     247     227     226
                 185     181      82      40       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1932    4947 
   Size:       1932    3015
 
FspI TGC'GCA

Cuts at:      0    1198    4473    4789    4891    4947 
   Size:       1198    3275     316     102      56

  Fragments arranged by size: 

                3275    1198     316     102      56
 
HaeII r_GCGC'y

Cuts at:      0     909     981    1461    1598    1795    2011    2525    3123 
   Size:        909      72     480     137     197     216     514     598

Cuts at:   3123    3613    4093    4241    4947 
   Size:        490     480     148     706

  Fragments arranged by size: 

                 909     706     598     514     490     480     480     216
                 197     148     137      72
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     166     198    2824    2856    3070    3102    3805    3837 
   Size:        166      32    2626      32     214      32     703      32

Cuts at:   3837    3844    3876    4099    4131    4947 
   Size:          7      32     223      32     816

  Fragments arranged by size: 

                2626     816     703     223     214     166      32      32
                  32      32      32      32       7
 
HincII GTy'rAC

Cuts at:      0    2315    4947 
   Size:       2315    2632
 
KasI G'GCGC_C

Cuts at:      0     905    1457    1791    4089    4947 
   Size:        905     552     334    2298     858

  Fragments arranged by size: 

                2298     905     858     552     334
 
KpnI G_GTAC'C

Cuts at:      0     624    4947 
   Size:        624    4323
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2522    2537    4208    4947 
   Size:       2522      15    1671     739

  Fragments arranged by size: 

                2522    1671     739      15
 
MscI TGG'CCA

Cuts at:      0    1426    4729    4849    4947 
   Size:       1426    3303     120      98

  Fragments arranged by size: 

                3303    1426     120      98
 
MslI CAynn'nnrTG

Cuts at:      0     152     967    4947 
   Size:        152     815    3980

  Fragments arranged by size: 

                3980     815     152
 
MspA1I CmG'CkG

Cuts at:      0     286     715    1217    1471    1751    1786    2472    3135 
   Size:        286     429     502     254     280      35     686     663

Cuts at:   3135    3414    3748    4567    4947 
   Size:        279     334     819     380

  Fragments arranged by size: 

                 819     686     663     502     429     380     334     286
                 280     279     254      35
 
NaeI GCC'GGC

Cuts at:      0    3154    4947 
   Size:       3154    1793
 
NarI GG'CG_CC

Cuts at:      0     906    1458    1792    4090    4947 
   Size:        906     552     334    2298     857

  Fragments arranged by size: 

                2298     906     857     552     334
 
NcoI C'CATG_G

Cuts at:      0    2265    4947 
   Size:       2265    2682
 
NgoMIV G'CCGG_C

Cuts at:      0    3152    4947 
   Size:       3152    1795
 
NheI G'CTAG_C

Cuts at:      0    4647    4947 
   Size:       4647     300
 
NspI r_CATG'y

Cuts at:      0    3682    4698    4890    4947 
   Size:       3682    1016     192      57

  Fragments arranged by size: 

                3682    1016     192      57
 
PasI CC'CwG_GG

Cuts at:      0      40    1822    2819    3242    4947 
   Size:         40    1782     997     423    1705

  Fragments arranged by size: 

                1782    1705     997     423      40
 
PciI A'CATG_T

Cuts at:      0    3678    4947 
   Size:       3678    1269
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     357    1422    2781    4947 
   Size:        357    1065    1359    2166

  Fragments arranged by size: 

                2166    1359    1065     357
 
PfoI T'CCnGG_A

Cuts at:      0     650    1450    1696    2343    3734    4947 
   Size:        650     800     246     647    1391    1213

  Fragments arranged by size: 

                1391    1213     800     650     647     246
 
PmlI CAC'GTG

Cuts at:      0    4374    4947 
   Size:       4374     573
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     856     888    2669    2701    4947 
   Size:        856      32    1781      32    2246

  Fragments arranged by size: 

                2246    1781     856      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     543     724     806    1763    3485    4947 
   Size:        543     181      82     957    1722    1462

  Fragments arranged by size: 

                1722    1462     957     543     181      82
 
PspOMI G'GGCC_C

Cuts at:      0    3237    4947 
   Size:       3237    1710
 
PspXI vC'TCGA_Gb

Cuts at:      0     794    3087    4947 
   Size:        794    2293    1860

  Fragments arranged by size: 

                2293    1860     794
 
PstI C_TGCA'G

Cuts at:      0    1409    1679    2933    3400    3722    4223    4611    4947 
   Size:       1409     270    1254     467     322     501     388     336

  Fragments arranged by size: 

                1409    1254     501     467     388     336     322     270
            
 
PvuII CAG'CTG

Cuts at:      0    1217    1751    2472    3748    4947 
   Size:       1217     534     721    1276    1199

  Fragments arranged by size: 

                1276    1217    1199     721     534
 
RsrII CG'GwC_CG

Cuts at:      0    2689    4947 
   Size:       2689    2258
 
SacI G_AGCT'C

Cuts at:      0     477    1090    2960    3088    3862    4947 
   Size:        477     613    1870     128     774    1085

  Fragments arranged by size: 

                1870    1085     774     613     477     128
 
SacII CC_GC'GG

Cuts at:      0    1472    1787    4947 
   Size:       1472     315    3160

  Fragments arranged by size: 

                3160    1472     315
 
SalI G'TCGA_C

Cuts at:      0    2313    4947 
   Size:       2313    2634
 
SanDI GG'GwC_CC

Cuts at:      0     724    4947 
   Size:        724    4223
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1334    3300    4947 
   Size:       1334    1966    1647

  Fragments arranged by size: 

                1966    1647    1334
 
SbfI CC_TGCA'GG

Cuts at:      0    3722    4223    4947 
   Size:       3722     501     724

  Fragments arranged by size: 

                3722     724     501
 
SexAI A'CCwGG_T

Cuts at:      0    3652    4853    4947 
   Size:       3652    1201      94

  Fragments arranged by size: 

                3652    1201      94
 
SfcI C'TryA_G

Cuts at:      0    1405    1675    2929    3396    3718    4219    4607    4947 
   Size:       1405     270    1254     467     322     501     388     340

  Fragments arranged by size: 

                1405    1254     501     467     388     340     322     270
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    3245    3709    4947 
   Size:       3245     464    1238

  Fragments arranged by size: 

                3245    1238     464
 
SfoI GGC'GCC

Cuts at:      0     907    1459    1793    4091    4947 
   Size:        907     552     334    2298     856

  Fragments arranged by size: 

                2298     907     856     552     334
 
SmaI CCC'GGG

Cuts at:      0     811    2215    2223    4264    4389    4947 
   Size:        811    1404       8    2041     125     558

  Fragments arranged by size: 

                2041    1404     811     558     125       8
 
SmlI C'TyrA_G

Cuts at:      0     794    1947    3087    3822    4455    4947 
   Size:        794    1153    1140     735     633     492

  Fragments arranged by size: 

                1153    1140     794     735     633     492
 
SspI AAT'ATT

Cuts at:      0    2340    4947 
   Size:       2340    2607
 
StuI AGG'CCT

Cuts at:      0    1203    4947 
   Size:       1203    3744
 
StyI C'CwwG_G

Cuts at:      0    1710    2265    3140    3209    3665    4763    4947 
   Size:       1710     555     875      69     456    1098     184

  Fragments arranged by size: 

                1710    1098     875     555     456     184      69
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      71    2928    2956    3100    3524    4036    4086    4947 
   Size:         71    2857      28     144     424     512      50     861

  Fragments arranged by size: 

                2857     861     512     424     144      71      50      28
            
 
TatI w'GTAC_w

Cuts at:      0    4676    4772    4877    4947 
   Size:       4676      96     105      70

  Fragments arranged by size: 

                4676     105      96      70
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     564     596    3435    3467    4947 
   Size:        564      32    2839      32    1480

  Fragments arranged by size: 

                2839    1480     564      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3561    4947 
   Size:       3561    1386
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     942    3381    3942    4819    4947 
   Size:        942    2439     561     877     128

  Fragments arranged by size: 

                2439     942     877     561     128
 
XhoI C'TCGA_G

Cuts at:      0     794    3087    4947 
   Size:        794    2293    1860

  Fragments arranged by size: 

                2293    1860     794
 
XmaI C'CCGG_G

Cuts at:      0     809    2213    2221    4262    4387    4947 
   Size:        809    1404       8    2041     125     560

  Fragments arranged by size: 

                2041    1404     809     560     125       8
 
ZraI GAC'GTC

Cuts at:      0    3263    4947 
   Size:       3263    1684
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI   AflIII     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NspI     PasI     PciI
    PflMI     PfoI     PmlI     PpiI    PpuMI   PspOMI    PspXI     PstI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI
     ZraI

 Enzymes that do not cut: 

     AclI     AfeI    AflII     AgeI     AhdI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI     BclI    BfrBI    BsaBI    BspHI    BsrGI
   BssHII    BstBI  BstZ17I     ClaI    CspCI     DraI   DraIII    EcoRI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     MluI     NdeI
     NotI     NruI     NsiI     PacI     PmeI    PshAI     PsiI     PsrI
     PvuI     ScaI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SwaI     XbaI     XmnI
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