Restriction map for pF1KSDA1519
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BamHI       BbeI        
BbrPI       BbvCI       BclI        BfaI        BfrI        
BglII       BlnI        BsaBI       BsaHI       BsaWI       
BseAI       BsiWI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrFI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Cfr10I      ClaI        CpoI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MfeI        MluI        MroI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    RcaI        
RsrII       SacII       SalI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        SspBI       SspI        SwaI        XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AflIII        881  ACACGT               A^C[GA][CT]GT
AocI         1150  CCTGAGG              CC^T[ACGT]AGG
Asp700I       277  GAAGTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1137  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          548  TGGCCA               TGG^CCA
BanI          963  GGTGCC               G^G[CT][GA]CC
BcgI         1173  GCAGAGGTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         912  GGATAC               GTATCC(6/5)
BsaAI         882  CACGTA               [CT]AC^GT[GA]
BsaI         1178  GGTCTC               GGTCTC(1/5)
BsiEI         405  CGATCG               CG[GA][CT]^CG
BsrDI         600  CATTGC               GCAATG(2/0)
BstXI         813  CCAGCTCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1150  CCTGAGG              CC^T[ACGT]AGG
DrdI          580  GACGAGGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          548  TGGCCA               [CT]^GGCC[GA]
Eco81I       1150  CCTGAGG              CC^T[ACGT]AGG
EcoRI        1375  GAATTC               G^AATTC
MluNI         548  TGGCCA               TGG^CCA
MscI          548  TGGCCA               TGG^CCA
MslI         1752  CACGCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI        1137  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         393  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI          405  CGATCG               CGAT^CG
SapI           51  GAAGAGC              GCTCTTC(1/4)
SbfI         1251  CCTGCAGG             CCTGCA^GG
SexAI        1283  ACCTGGT              A^CC[AT]GGT
SmlI         1879  CTCAAG               C^T[CT][GA]AG
Sse8387I     1251  CCTGCAGG             CCTGCA^GG
TaqI          470  TCGA                 T^CGA
Tth111I      1137  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       470  TCGA                 T^CGA
Van91I        393  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          551  CCAAGCAGAGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          277  GAAGTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcsI         1375  GAATTC               [GA]^AATT[CT]
             1650  AAATTT              
Alw44I        373  GTGCAC               G^TGCAC
              534  GTGCAC              
AlwI         1740  GATCC                GGATC(4/5)
              177  GGATC               
ApaLI         373  GTGCAC               G^TGCAC
              534  GTGCAC              
ApoI         1375  GAATTC               [GA]^AATT[CT]
             1650  AAATTT              
BbsI         1242  GAAGAC               GAAGAC(2/6)
             1884  GAAGAC              
BglI         1249  GCCCTGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1919  GCCATGGAGGC         
BpuAI        1242  GAAGAC               GAAGAC(2/6)
             1884  GAAGAC              
BsrI         1862  ACTGG                ACTGG(1/-1)
              729  CCAGT               
BstYI         177  GGATCT               [GA]^GATC[CT]
             1739  AGATCC              
EcoNI        1338  CCTTGCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1803  CCTGCTGCAGG         
HaeII        1260  GGCGCT               [GA]GCGC^[CT]
             1912  AGCGCC              
HgaI         1272  GACGC                GACGC(5/10)
              485  GCGTC               
MflI          177  GGATCT               [GA]^GATC[CT]
             1739  AGATCC              
MspA1I        397  CAGCTG               C[AC]G^C[GT]G
              774  CAGCTG              
PvuII         397  CAGCTG               CAG^CTG
              774  CAGCTG              
TfiI          691  GAATC                G^A[AT]TC
             1022  GATTC               
Tsp509I      1376  AATT                 ^AATT
             1651  AATT                
XhoII         177  GGATCT               [GA]^GATC[CT]
             1739  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
AspHI       AvaI        AvaII       BanII       BbvI        
BcnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspMI       BsrBI       BssKI       
BssSI       BstAPI      BstF5I      BstNI       BstUI       
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       PstI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
StuI        StyI        Tru9I       TseI        Tsp45I      
TspRI       XmaCI       XmaI        
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