Restriction map for pF1KSDA1519
 (Linear) (Six-base) MAPSORT of: KIAA1519.seq  Check: 1490  from: 1  to: 1941



KIAA1519.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     386    1811    1941 
   Size:        386    1425     130

  Fragments arranged by size: 

                1425     386     130
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     633    1732    1941 
   Size:         73     560    1099     209

  Fragments arranged by size: 

                1099     560     209      73
 
AflIII A'CryG_T

Cuts at:      0     881    1941 
   Size:        881    1060
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1333    1941 
   Size:       1333     608
 
AlwNI CAG_nnn'CTG

Cuts at:      0     222     399     561    1298    1636    1722    1761    1815 
   Size:        222     177     162     737     338      86      39      54

Cuts at:   1815    1941 
   Size:        126

  Fragments arranged by size: 

                 737     338     222     177     162     126      86      54
                  39
 
ApaLI G'TGCA_C

Cuts at:      0     373     534    1941 
   Size:        373     161    1407

  Fragments arranged by size: 

                1407     373     161
 
ApoI r'AATT_y

Cuts at:      0    1375    1650    1941 
   Size:       1375     275     291

  Fragments arranged by size: 

                1375     291     275
 
AvaI C'yCGr_G

Cuts at:      0     634    1181    1213    1234    1487    1941 
   Size:        634     547      32      21     253     454

  Fragments arranged by size: 

                 634     547     454     253      32      21
 
BanI G'GyrC_C

Cuts at:      0     963    1941 
   Size:        963     978
 
BanII G_rGCy'C

Cuts at:      0     308     712    1751    1880    1941 
   Size:        308     404    1039     129      61

  Fragments arranged by size: 

                1039     404     308     129      61
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1249    1891    1941 
   Size:       1249     642      50

  Fragments arranged by size: 

                1249     642      50
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1162    1196    1941 
   Size:       1162      34     745

  Fragments arranged by size: 

                1162     745      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     906    1941 
   Size:        906    1035
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1255    1925    1941 
   Size:       1255     670      16

  Fragments arranged by size: 

                1255     670      16
 
BlpI GC'TnA_GC

Cuts at:      0      28      83    1466    1816    1941 
   Size:         28      55    1383     350     125

  Fragments arranged by size: 

                1383     350     125      55      28
 
Bme1580I G_kGCm'C

Cuts at:      0     377     538     870    1088    1941 
   Size:        377     161     332     218     853

  Fragments arranged by size: 

                 853     377     332     218     161
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     690    1941 
   Size:        690    1251
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     376     814    1288    1456    1744    1884    1941 
   Size:        226     150     438     474     168     288     140      57

  Fragments arranged by size: 

                 474     438     288     226     168     150     140      57
            
 
Bpu10I CC'TnA_GC

Cuts at:      0      60    1099    1343    1388    1941 
   Size:         60    1039     244      45     553

  Fragments arranged by size: 

                1039     553     244      60      45
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1864    1941 
   Size:       1864      77
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1184    1941 
   Size:       1184     757
 
BsaAI yAC'GTr

Cuts at:      0     884    1941 
   Size:        884    1057
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     247     277     889     919     947     977    1162    1192 
   Size:        247      30     612      30      28      30     185      30

Cuts at:   1192    1429    1459    1941 
   Size:        237      30     482

  Fragments arranged by size: 

                 612     482     247     237     185      30      30      30
                  30      30      28
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      49     255     780     783    1183    1941 
   Size:         49     206     525       3     400     758

  Fragments arranged by size: 

                 758     525     400     206      49       3
 
BseYI C'CCAG_C

Cuts at:      0     489    1298    1941 
   Size:        489     809     643

  Fragments arranged by size: 

                 809     643     489
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1014    1303    1501    1649    1941 
   Size:       1014     289     198     148     292

  Fragments arranged by size: 

                1014     292     289     198     148
 
BsiEI CG_ry'CG

Cuts at:      0     408    1941 
   Size:        408    1533
 
BsiHKAI G_wGCw'C

Cuts at:      0     308     377     538     643    1730    1751    1880    1941 
   Size:        308      69     161     105    1087      21     129      61

  Fragments arranged by size: 

                1087     308     161     129     105      69      61      21
            
 
BsmI GAATG_Cn'

Cuts at:      0     212    1013    1333    1941 
   Size:        212     801     320     608

  Fragments arranged by size: 

                 801     608     320     212
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     754    1214    1264    1941 
   Size:        754     460      50     677

  Fragments arranged by size: 

                 754     677     460      50
 
Bsp1286I G_dGCh'C

Cuts at:      0     308     377     538     643     712     870    1088    1730 
   Size:        308      69     161     105      69     158     218     642

Cuts at:   1730    1751    1880    1941 
   Size:         21     129      61

  Fragments arranged by size: 

                 642     308     218     161     158     129     105      69
                  69      61      21
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     386    1301    1811    1941 
   Size:        386     915     510     130

  Fragments arranged by size: 

                 915     510     386     130
 
BsrBI CCG'CTC

Cuts at:      0      56     429     847    1941 
   Size:         56     373     418    1094

  Fragments arranged by size: 

                1094     418     373      56
 
BsrDI GCAATG_nn'

Cuts at:      0     599    1941 
   Size:        599    1342
 
BssSI C'ACGA_G

Cuts at:      0     307     642     677    1941 
   Size:        307     335      35    1264

  Fragments arranged by size: 

                1264     335     307      35
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     785    1339    1636    1761    1941 
   Size:        785     554     297     125     180

  Fragments arranged by size: 

                 785     554     297     180     125
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     820    1941 
   Size:        820    1121
 
BstYI r'GATC_y

Cuts at:      0     177    1739    1941 
   Size:        177    1562     202

  Fragments arranged by size: 

                1562     202     177
 
Bsu36I CC'TnA_GG

Cuts at:      0    1151    1941 
   Size:       1151     790
 
BtgI C'CryG_G

Cuts at:      0     335     908    1889    1920    1941 
   Size:        335     573     981      31      21

  Fragments arranged by size: 

                 981     573     335      31      21
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1417    1941 
   Size:       1417     524
 
BtsI GCAGTG_nn'

Cuts at:      0    1634    1941 
   Size:       1634     307
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     586    1941 
   Size:        586    1355
 
EaeI y'GGCC_r

Cuts at:      0     548    1941 
   Size:        548    1393
 
EarI CTCTTCn'nnn_

Cuts at:      0      46     936    1568    1618    1678    1941 
   Size:         46     890     632      50      60     263

  Fragments arranged by size: 

                 890     632     263      60      50      46
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     821    1525    1941 
   Size:        821     704     416

  Fragments arranged by size: 

                 821     704     416
 
EcoICRI GAG'CTC

Cuts at:      0     306    1749    1878    1941 
   Size:        306    1443     129      63

  Fragments arranged by size: 

                1443     306     129      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     226     376     633     814    1288    1456    1732 
   Size:         73     153     150     257     181     474     168     276

Cuts at:   1732    1744    1884    1941 
   Size:         12     140      57

  Fragments arranged by size: 

                 474     276     257     181     168     153     150     140
                  73      57      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1342    1807    1941 
   Size:       1342     465     134

  Fragments arranged by size: 

                1342     465     134
 
EcoO109I rG'GnC_Cy

Cuts at:      0      64     618    1076    1136    1313    1941 
   Size:         64     554     458      60     177     628

  Fragments arranged by size: 

                 628     554     458     177      64      60
 
EcoRI G'AATT_C

Cuts at:      0    1375    1941 
   Size:       1375     566
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      75    1941 
   Size:         75    1866
 
HaeII r_GCGC'y

Cuts at:      0    1264    1916    1941 
   Size:       1264     652      25

  Fragments arranged by size: 

                1264     652      25
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      76     108     228     260     913     945    1128    1160 
   Size:         76      32     120      32     653      32     183      32

Cuts at:   1160    1162    1194    1408    1429    1440    1461    1941 
   Size:          2      32     214      21      11      21     480

  Fragments arranged by size: 

                 653     480     214     183     120      76      32      32
                  32      32      32      21      21      11       2
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     923     996    1941 
   Size:        923      73     945

  Fragments arranged by size: 

                 945     923      73
 
MscI TGG'CCA

Cuts at:      0     550    1941 
   Size:        550    1391
 
MslI CAynn'nnrTG

Cuts at:      0    1756    1941 
   Size:       1756     185
 
MspA1I CmG'CkG

Cuts at:      0     399     776    1941 
   Size:        399     377    1165

  Fragments arranged by size: 

                1165     399     377
 
NcoI C'CATG_G

Cuts at:      0     908    1889    1920    1941 
   Size:        908     981      31      21

  Fragments arranged by size: 

                 981     908      31      21
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     399    1941 
   Size:        399    1542
 
PfoI T'CCnGG_A

Cuts at:      0     105    1265    1742    1941 
   Size:        105    1160     477     199

  Fragments arranged by size: 

                1160     477     199     105
 
PpuMI rG'GwC_Cy

Cuts at:      0     618    1076    1136    1313    1941 
   Size:        618     458      60     177     628

  Fragments arranged by size: 

                 628     618     458     177      60
 
PstI C_TGCA'G

Cuts at:      0     781    1256    1312    1641    1766    1811    1941 
   Size:        781     475      56     329     125      45     130

  Fragments arranged by size: 

                 781     475     329     130     125      56      45
 
PvuI CG_AT'CG

Cuts at:      0     408    1941 
   Size:        408    1533
 
PvuII CAG'CTG

Cuts at:      0     399     776    1941 
   Size:        399     377    1165

  Fragments arranged by size: 

                1165     399     377
 
SacI G_AGCT'C

Cuts at:      0     308    1751    1880    1941 
   Size:        308    1443     129      61

  Fragments arranged by size: 

                1443     308     129      61
 
SapI GCTCTTCn'nnn_

Cuts at:      0      46    1941 
   Size:         46    1895
 
SbfI CC_TGCA'GG

Cuts at:      0    1256    1941 
   Size:       1256     685
 
SexAI A'CCwGG_T

Cuts at:      0    1283    1941 
   Size:       1283     658
 
SfcI C'TryA_G

Cuts at:      0     777    1252    1308    1637    1762    1807    1941 
   Size:        777     475      56     329     125      45     134

  Fragments arranged by size: 

                 777     475     329     134     125      56      45
 
SmaI CCC'GGG

Cuts at:      0     636    1215    1236    1489    1941 
   Size:        636     579      21     253     452

  Fragments arranged by size: 

                 636     579     452     253      21
 
SmlI C'TyrA_G

Cuts at:      0    1879    1941 
   Size:       1879      62
 
StuI AGG'CCT

Cuts at:      0     544     630     859    1156    1936    1941 
   Size:        544      86     229     297     780       5

  Fragments arranged by size: 

                 780     544     297     229      86       5
 
StyI C'CwwG_G

Cuts at:      0     194     545     805     908    1088    1889    1920    1941 
   Size:        194     351     260     103     180     801      31      21

  Fragments arranged by size: 

                 801     351     260     194     180     103      31      21
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     145     914    1207    1399    1941 
   Size:        145     769     293     192     542

  Fragments arranged by size: 

                 769     542     293     192     145
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     889     921     947     979    1941 
   Size:        889      32      26      32     962

  Fragments arranged by size: 

                 962     889      32      32      26
 
Tth111I GACn'n_nGTC

Cuts at:      0    1140    1941 
   Size:       1140     801
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     558    1941 
   Size:        558    1383
 
XmaI C'CCGG_G

Cuts at:      0     634    1213    1234    1487    1941 
   Size:        634     579      21     253     454

  Fragments arranged by size: 

                 634     579     454     253      21
 
XmnI GAAnn'nnTTC

Cuts at:      0     281    1941 
   Size:        281    1660
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AlfI    AlwNI    ApaLI     ApoI     AvaI
     BanI    BanII     BbsI     BcgI    BciVI     BglI     BlpI Bme1580I
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrDI    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I     MmeI     MscI
     MslI   MspA1I     NcoI    PflMI     PfoI    PpuMI     PstI     PvuI
    PvuII     SacI     SapI     SbfI    SexAI     SfcI     SmaI     SmlI
     StuI     StyI    TaqII     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BclI    BfrBI    BglII    BmgBI     BmrI
     BmtI    BsaBI    BsaHI    BsaWI    BsiWI    BspEI    BspHI    BsrFI
    BsrGI   BssHII    BstBI   BstEII  BstZ17I     ClaI    CspCI     DraI
   DraIII     EagI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI    RsrII    SacII     SalI    SanDI     ScaI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI     XbaI     XhoI     ZraI
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