Restriction map for
pF1KSDA1504
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII AccII AccIII AciI AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BbvI BcgI BciVI BclI BcnI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
BsoBI BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT22I FauI
FbaI Fnu4HI FseI FspI HaeII
HapII HhaI HinP1I HpaII ItaI
KasI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI MwoI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI TaqI TseI TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 796 GGTACC G^GTACC
AccI 472 GTAGAC GT^[AC][GT]AC
AcyI 917 GGCGTC G[GA]^CG[CT]C
AocI 460 CCTGAGG CC^T[ACGT]AGG
AseI 534 ATTAAT AT^TAAT
AsnI 534 ATTAAT AT^TAAT
Asp718I 796 GGTACC G^GTACC
AspHI 390 GAGCTC G[AT]GC[AT]^C
BanI 796 GGTACC G^G[CT][GA]CC
BanII 390 GAGCTC G[GA]GC[CT]^C
BglII 1009 AGATCT A^GATCT
BmyI 390 GAGCTC G[AGT]GC[ACT]^C
BsaHI 917 GGCGTC G[GA]^CG[CT]C
BseRI 596 CTCCTC GAGGAG(10/8)
BsiHKAI 390 GAGCTC G[AT]GC[AT]^C
BsiYI 1042 CCGAACCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1042 CCGAACCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 919 CGTCTC CGTCTC(1/5)
Bsp1286I 390 GAGCTC G[AGT]GC[ACT]^C
BsrI 895 CCAGT ACTGG(1/-1)
BstXI 1148 CCAACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 460 CCTGAGG CC^T[ACGT]AGG
Cac8I 447 GCCAGC GC[ACGT]^[ACGT]GC
DraII 27 GGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 612 GACTTCTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 460 CCTGAGG CC^T[ACGT]AGG
EcoO109I 27 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 246 GAATTC G^AATTC
HaeIII 1262 GGCC GG^CC
HgaI 918 GCGTC GACGC(5/10)
Hin1I 917 GGCGTC G[GA]^CG[CT]C
HincII 946 GTTAAC GT[CT]^[GA]AC
HindII 946 GTTAAC GT[CT]^[GA]AC
HindIII 824 AAGCTT A^AGCTT
HpaI 946 GTTAAC GTT^AAC
HphI 250 TCACC GGTGA(8/7)
KpnI 796 GGTACC GGTAC^C
MaeII 470 ACGT A^CGT
MslI 1210 CATATTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PpuMI 27 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 534 ATTAAT AT^TAAT
SacI 390 GAGCTC GAGCT^C
SfcI 941 CTACAG C^T[GA][CT]AG
StuI 1261 AGGCCT AGG^CCT
TfiI 807 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AluI 391 AGCT AG^CT
825 AGCT
AlwI 676 GATCC GGATC(4/5)
129 GGATC
Asp700I 76 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
902 GAAGTTCTTC
AvaII 28 GGTCC G^G[AT]CC
1039 GGACC
BbsI 164 GTCTTC GAAGAC(2/6)
1057 GTCTTC
BpuAI 164 GTCTTC GAAGAC(2/6)
1057 GTCTTC
Bsp1407I 478 TGTACA T^GTACA
629 TGTACA
BsrGI 478 TGTACA T^GTACA
629 TGTACA
BstYI 675 AGATCC [GA]^GATC[CT]
1009 AGATCT
Cfr13I 28 GGTCC G^G[ACGT]CC
1039 GGACC
DsaI 961 CCATGG C^C[GA][CT]GG
1047 CCATGG
EarI 782 CTCTTC CTCTTC(1/4)
966 GAAGAG
EcoT14I 961 CCATGG C^C[AT][AT]GG
1047 CCATGG
Ksp632I 782 CTCTTC CTCTTC(1/4)
966 GAAGAG
MflI 675 AGATCC [GA]^GATC[CT]
1009 AGATCT
NcoI 961 CCATGG C^CATGG
1047 CCATGG
Sau96I 28 GGTCC G^G[ACGT]CC
1039 GGACC
SfaNI 20 GCATC GCATC(5/9)
451 GCATC
SspBI 478 TGTACA T^GTACA
629 TGTACA
StyI 961 CCATGG C^C[AT][AT]GG
1047 CCATGG
Tsp45I 494 GTGAC ^GT[GC]AC
1217 GTGAC
XhoII 675 AGATCC [GA]^GATC[CT]
1009 AGATCT
XmnI 76 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
902 GAAGTTCTTC
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI AfaI ApoI BpmI BsaJI
BsmAI BsmFI BspHI BssKI BstF5I
BstNI DdeI DpnII EcoRII FokI
HinfI MaeIII MboI MboII MnlI
MseI MvaI NdeII NlaIII NlaIV
PleI RcaI RsaI Sau3AI ScrFI
Tru9I Tsp509I