Restriction map for pF1KSDA1504
 (Linear) (Six-base) MAPSORT of: KIAA1504.seq  Check: 1544  from: 1  to: 1278



KIAA1504.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AccI GT'mk_AC

Cuts at:      0     473    1278 
   Size:        473     805
 
Acc65I G'GTAC_C

Cuts at:      0     796    1278 
   Size:        796     482
 
ApoI r'AATT_y

Cuts at:      0     105     246    1224    1245    1278 
   Size:        105     141     978      21      33

  Fragments arranged by size: 

                 978     141     105      33      21
 
AseI AT'TA_AT

Cuts at:      0     535    1278 
   Size:        535     743
 
BanI G'GyrC_C

Cuts at:      0     796    1278 
   Size:        796     482
 
BanII G_rGCy'C

Cuts at:      0     394    1278 
   Size:        394     884
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     157    1050    1278 
   Size:        157     893     228

  Fragments arranged by size: 

                 893     228     157
 
BglII A'GATC_T

Cuts at:      0    1009    1278 
   Size:       1009     269
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     889    1278 
   Size:        889     389
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     413    1278 
   Size:        413     865
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     545     933    1181    1278 
   Size:        545     388     248      97

  Fragments arranged by size: 

                 545     388     248      97
 
BsaHI Gr'CG_yC

Cuts at:      0     918    1278 
   Size:        918     360
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     587    1278 
   Size:        587     691
 
BseYI C'CCAG_C

Cuts at:      0      31    1278 
   Size:         31    1247
 
BsiHKAI G_wGCw'C

Cuts at:      0     394    1278 
   Size:        394     884
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     925    1278 
   Size:        925     353
 
Bsp1286I G_dGCh'C

Cuts at:      0     394    1278 
   Size:        394     884
 
BspHI T'CATG_A

Cuts at:      0      72     567     861    1278 
   Size:         72     495     294     417

  Fragments arranged by size: 

                 495     417     294      72
 
BsrGI T'GTAC_A

Cuts at:      0     478     629    1278 
   Size:        478     151     649

  Fragments arranged by size: 

                 649     478     151
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1155    1278 
   Size:       1155     123
 
BstYI r'GATC_y

Cuts at:      0     675    1009    1278 
   Size:        675     334     269

  Fragments arranged by size: 

                 675     334     269
 
Bsu36I CC'TnA_GG

Cuts at:      0     461    1278 
   Size:        461     817
 
BtgI C'CryG_G

Cuts at:      0     961    1047    1278 
   Size:        961      86     231

  Fragments arranged by size: 

                 961     231      86
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     618    1278 
   Size:        618     660
 
EarI CTCTTCn'nnn_

Cuts at:      0     788     961    1278 
   Size:        788     173     317

  Fragments arranged by size: 

                 788     317     173
 
EcoICRI GAG'CTC

Cuts at:      0     392    1278 
   Size:        392     886
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     545     933    1181    1278 
   Size:        545     388     248      97

  Fragments arranged by size: 

                 545     388     248      97
 
EcoO109I rG'GnC_Cy

Cuts at:      0      28    1278 
   Size:         28    1250
 
EcoRI G'AATT_C

Cuts at:      0     246    1278 
   Size:        246    1032
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     181     923    1278 
   Size:        181     742     355

  Fragments arranged by size: 

                 742     355     181
 
HincII GTy'rAC

Cuts at:      0     948    1278 
   Size:        948     330
 
HindIII A'AGCT_T

Cuts at:      0     824    1278 
   Size:        824     454
 
HpaI GTT'AAC

Cuts at:      0     948    1278 
   Size:        948     330
 
KpnI G_GTAC'C

Cuts at:      0     800    1278 
   Size:        800     478
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     382     985    1278 
   Size:        382     603     293

  Fragments arranged by size: 

                 603     382     293
 
MslI CAynn'nnrTG

Cuts at:      0    1214    1278 
   Size:       1214      64
 
NcoI C'CATG_G

Cuts at:      0     961    1047    1278 
   Size:        961      86     231

  Fragments arranged by size: 

                 961     231      86
 
PfoI T'CCnGG_A

Cuts at:      0     910    1278 
   Size:        910     368
 
PpuMI rG'GwC_Cy

Cuts at:      0      28    1278 
   Size:         28    1250
 
PsiI TTA'TAA

Cuts at:      0     656    1278 
   Size:        656     622
 
SacI G_AGCT'C

Cuts at:      0     394    1278 
   Size:        394     884
 
SanDI GG'GwC_CC

Cuts at:      0      28    1278 
   Size:         28    1250
 
SfcI C'TryA_G

Cuts at:      0     941    1278 
   Size:        941     337
 
StuI AGG'CCT

Cuts at:      0    1263    1278 
   Size:       1263      15
 
StyI C'CwwG_G

Cuts at:      0     961    1047    1278 
   Size:        961      86     231

  Fragments arranged by size: 

                 961     231      86
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1056    1278 
   Size:       1056     222
 
TatI w'GTAC_w

Cuts at:      0     478     629    1278 
   Size:        478     151     649

  Fragments arranged by size: 

                 649     478     151
 
XmnI GAAnn'nnTTC

Cuts at:      0      80     906    1278 
   Size:         80     826     372

  Fragments arranged by size: 

                 826     372      80
 

 Enzymes that do cut:

     AccI   Acc65I     ApoI     AseI     BanI    BanII     BbsI    BglII
     BmrI     BplI     BpmI    BsaHI    BseRI    BseYI  BsiHKAI    BsmBI
 Bsp1286I    BspHI    BsrGI    BstXI    BstYI   Bsu36I     BtgI     DrdI
     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI   HincII  HindIII
     HpaI     KpnI     MmeI     MslI     NcoI     PfoI    PpuMI     PsiI
     SacI    SanDI     SfcI     StuI     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI     BsgI
    BsiEI    BsiWI     BsmI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EciI    EcoNI
    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I     KasI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com