Restriction map for pF1KSDA1470
 (Linear) (Six-base) MAPSORT of: KIAA1470.seq  Check: 1232  from: 1  to: 1566



KIAA1470.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1189    1566 
   Size:       1189     377
 
AfeI AGC'GCT

Cuts at:      0     121    1566 
   Size:        121    1445
 
AlwNI CAG_nnn'CTG

Cuts at:      0     724     972    1566 
   Size:        724     248     594

  Fragments arranged by size: 

                 724     594     248
 
ApaI G_GGCC'C

Cuts at:      0      74     186     416    1566 
   Size:         74     112     230    1150

  Fragments arranged by size: 

                1150     230     112      74
 
ApoI r'AATT_y

Cuts at:      0     402     790    1292    1566 
   Size:        402     388     502     274

  Fragments arranged by size: 

                 502     402     388     274
 
AvaI C'yCGr_G

Cuts at:      0      45     115     262     462     887    1566 
   Size:         45      70     147     200     425     679

  Fragments arranged by size: 

                 679     425     200     147      70      45
 
BanI G'GyrC_C

Cuts at:      0     428     836    1566 
   Size:        428     408     730

  Fragments arranged by size: 

                 730     428     408
 
BanII G_rGCy'C

Cuts at:      0      46      74     176     186     416     469     664    1566 
   Size:         46      28     102      10     230      53     195     902

  Fragments arranged by size: 

                 902     230     195     102      53      46      28      10
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     958    1044    1403    1566 
   Size:        958      86     359     163

  Fragments arranged by size: 

                 958     359     163      86
 
BglI GCCn_nnn'nGGC

Cuts at:      0      95     784    1085    1566 
   Size:         95     689     301     481

  Fragments arranged by size: 

                 689     481     301      95
 
BglII A'GATC_T

Cuts at:      0    1163    1566 
   Size:       1163     403
 
Bme1580I G_kGCm'C

Cuts at:      0      74     186     416     841     894    1566 
   Size:         74     112     230     425      53     672

  Fragments arranged by size: 

                 672     425     230     112      74      53
 
BmgBI CAC'GTC

Cuts at:      0    1002    1566 
   Size:       1002     564
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     348    1393    1566 
   Size:        348    1045     173

  Fragments arranged by size: 

                1045     348     173
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      60    1566 
   Size:         60    1506
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190    1308    1566 
   Size:        190    1118     258

  Fragments arranged by size: 

                1118     258     190
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     465    1566 
   Size:        465    1101
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     470     500     507     537    1168    1198    1468    1498 
   Size:        470      30       7      30     631      30     270      30

Cuts at:   1498    1566 
   Size:         68

  Fragments arranged by size: 

                 631     470     270      68      30      30      30      30
                   7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      49     478    1566 
   Size:         49     429    1088

  Fragments arranged by size: 

                1088     429      49
 
BseYI C'CCAG_C

Cuts at:      0     518     543     699     730     855    1083    1355    1566 
   Size:        518      25     156      31     125     228     272     211

  Fragments arranged by size: 

                 518     272     228     211     156     125      31      25
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     461    1291    1566 
   Size:        461     830     275

  Fragments arranged by size: 

                 830     461     275
 
BsiEI CG_ry'CG

Cuts at:      0     214     245    1566 
   Size:        214      31    1321

  Fragments arranged by size: 

                1321     214      31
 
BsiHKAI G_wGCw'C

Cuts at:      0      46     176     269    1348    1566 
   Size:         46     130      93    1079     218

  Fragments arranged by size: 

                1079     218     130      93      46
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     992    1566 
   Size:        992     574
 
Bsp1286I G_dGCh'C

Cuts at:      0      46      74     176     186     269     416     469     664 
   Size:         46      28     102      10      83     147      53     195

Cuts at:    664     841     894    1348    1566 
   Size:        177      53     454     218

  Fragments arranged by size: 

                 454     218     195     177     147     102      83      53
                  53      46      28      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1189    1452    1566 
   Size:       1189     263     114

  Fragments arranged by size: 

                1189     263     114
 
BsrBI CCG'CTC

Cuts at:      0     111    1566 
   Size:        111    1455
 
BsrFI r'CCGG_y

Cuts at:      0     213    1079    1566 
   Size:        213     866     487

  Fragments arranged by size: 

                 866     487     213
 
BsrGI T'GTAC_A

Cuts at:      0     749    1566 
   Size:        749     817
 
BssHII G'CGCG_C

Cuts at:      0     201    1566 
   Size:        201    1365
 
BssSI C'ACGA_G

Cuts at:      0     468    1566 
   Size:        468    1098
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     424    1566 
   Size:        424    1142
 
BstYI r'GATC_y

Cuts at:      0    1163    1566 
   Size:       1163     403
 
BtgI C'CryG_G

Cuts at:      0     245     497    1469    1566 
   Size:        245     252     972      97

  Fragments arranged by size: 

                 972     252     245      97
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     867    1566 
   Size:        867     699
 
BtsI GCAGTG_nn'

Cuts at:      0      56    1274    1415    1566 
   Size:         56    1218     141     151

  Fragments arranged by size: 

                1218     151     141      56
 
DraIII CAC_nnn'GTG

Cuts at:      0     606    1566 
   Size:        606     960
 
EaeI y'GGCC_r

Cuts at:      0     211     242     756     935    1077    1331    1566 
   Size:        211      31     514     179     142     254     235

  Fragments arranged by size: 

                 514     254     235     211     179     142      31
 
EagI C'GGCC_G

Cuts at:      0     211     242    1566 
   Size:        211      31    1324

  Fragments arranged by size: 

                1324     211      31
 
EarI CTCTTCn'nnn_

Cuts at:      0    1309    1566 
   Size:       1309     257
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     645    1566 
   Size:        645     921
 
EcoICRI GAG'CTC

Cuts at:      0      44     174    1566 
   Size:         44     130    1392

  Fragments arranged by size: 

                1392     130      44
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190    1308    1566 
   Size:        190    1118     258

  Fragments arranged by size: 

                1118     258     190
 
EcoO109I rG'GnC_Cy

Cuts at:      0     182     183     412     413    1275    1566 
   Size:        182       1     229       1     862     291

  Fragments arranged by size: 

                 862     291     229     182       1       1
 
EcoRI G'AATT_C

Cuts at:      0     790    1292    1566 
   Size:        790     502     274

  Fragments arranged by size: 

                 790     502     274
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     331    1068    1566 
   Size:        331     737     498

  Fragments arranged by size: 

                 737     498     331
 
HaeII r_GCGC'y

Cuts at:      0     123    1016    1566 
   Size:        123     893     550

  Fragments arranged by size: 

                 893     550     123
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     507     539     923     955    1566 
   Size:        507      32     384      32     611

  Fragments arranged by size: 

                 611     507     384      32      32
 
MscI TGG'CCA

Cuts at:      0     937    1566 
   Size:        937     629
 
MslI CAynn'nnrTG

Cuts at:      0     422    1327    1566 
   Size:        422     905     239

  Fragments arranged by size: 

                 905     422     239
 
MspA1I CmG'CkG

Cuts at:      0     137     152     543     699     855     897    1355    1566 
   Size:        137      15     391     156     156      42     458     211

  Fragments arranged by size: 

                 458     391     211     156     156     137      42      15
            
 
NaeI GCC'GGC

Cuts at:      0     215    1081    1566 
   Size:        215     866     485

  Fragments arranged by size: 

                 866     485     215
 
NcoI C'CATG_G

Cuts at:      0    1469    1566 
   Size:       1469      97
 
NgoMIV G'CCGG_C

Cuts at:      0     213    1079    1566 
   Size:        213     866     487

  Fragments arranged by size: 

                 866     487     213
 
NspI r_CATG'y

Cuts at:      0     626    1566 
   Size:        626     940
 
PasI CC'CwG_GG

Cuts at:      0    1144    1566 
   Size:       1144     422
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     642    1026    1355    1566 
   Size:        642     384     329     211

  Fragments arranged by size: 

                 642     384     329     211
 
PfoI T'CCnGG_A

Cuts at:      0    1031    1566 
   Size:       1031     535
 
PpuMI rG'GwC_Cy

Cuts at:      0    1275    1566 
   Size:       1275     291
 
PshAI GACnn'nnGTC

Cuts at:      0     457    1566 
   Size:        457    1109
 
PspOMI G'GGCC_C

Cuts at:      0      70     182     412    1566 
   Size:         70     112     230    1154

  Fragments arranged by size: 

                1154     230     112      70
 
PstI C_TGCA'G

Cuts at:      0     127     621    1422    1566 
   Size:        127     494     801     144

  Fragments arranged by size: 

                 801     494     144     127
 
PvuII CAG'CTG

Cuts at:      0     543     699     855    1355    1566 
   Size:        543     156     156     500     211

  Fragments arranged by size: 

                 543     500     211     156     156
 
SacI G_AGCT'C

Cuts at:      0      46     176    1566 
   Size:         46     130    1390

  Fragments arranged by size: 

                1390     130      46
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1309    1566 
   Size:       1309     257
 
SfcI C'TryA_G

Cuts at:      0     123     617    1418    1566 
   Size:        123     494     801     148

  Fragments arranged by size: 

                 801     494     148     123
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1085    1566 
   Size:       1085     481
 
SmaI CCC'GGG

Cuts at:      0     889    1566 
   Size:        889     677
 
SpeI A'CTAG_T

Cuts at:      0     918    1566 
   Size:        918     648
 
SrfI GCCC'GGGC

Cuts at:      0     889    1566 
   Size:        889     677
 
StyI C'CwwG_G

Cuts at:      0     272     641     705    1469    1487    1566 
   Size:        272     369      64     764      18      79

  Fragments arranged by size: 

                 764     369     272      79      64      18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     385     416     824    1195    1566 
   Size:        385      31     408     371     371

  Fragments arranged by size: 

                 408     385     371     371      31
 
TatI w'GTAC_w

Cuts at:      0     749    1566 
   Size:        749     817
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     470     502    1566 
   Size:        470      32    1064

  Fragments arranged by size: 

                1064     470      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1168    1566 
   Size:       1168     398
 
XmaI C'CCGG_G

Cuts at:      0     887    1566 
   Size:        887     679
 
XmnI GAAnn'nnTTC

Cuts at:      0     920    1566 
   Size:        920     646
 

 Enzymes that do cut:

     AarI     AfeI    AlwNI     ApaI     ApoI     AvaI     BanI    BanII
     BbsI     BglI    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI
     BsaI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstXI
    BstYI     BtgI    BtgZI     BtsI   DraIII     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI    HaeII    Hin4I
     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI     PasI
    PflMI     PfoI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI
     SapI     SfcI     SfiI     SmaI     SpeI     SrfI     StyI    TaqII
     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BlpI     BmtI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsiWI     BsmI    BspEI    BspHI    BsrDI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI     DrdI    EcoNI
    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NarI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI     PsiI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SphI
     SspI     StuI     SwaI  Tth111I     XbaI     XhoI     ZraI
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