Restriction map for pF1KSDA1465
 (Linear) (Six-base) MAPSORT of: KIAA1465.seq  Check: 421  from: 1  to: 2235



KIAA1465.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AatII G_ACGT'C

Cuts at:      0     221    2235 
   Size:        221    2014
 
AccI GT'mk_AC

Cuts at:      0    1055    2235 
   Size:       1055    1180
 
Acc65I G'GTAC_C

Cuts at:      0    1681    2235 
   Size:       1681     554
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     805    2235 
   Size:        805    1430
 
AfeI AGC'GCT

Cuts at:      0    1372    2235 
   Size:       1372     863
 
AflIII A'CryG_T

Cuts at:      0    1104    1412    2235 
   Size:       1104     308     823

  Fragments arranged by size: 

                1104     823     308
 
AgeI A'CCGG_T

Cuts at:      0     855    2235 
   Size:        855    1380
 
AhdI GACnn_n'nnGTC

Cuts at:      0     167    2235 
   Size:        167    2068
 
AleI CACnn'nnGTG

Cuts at:      0     598    2235 
   Size:        598    1637
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     728     763    2235 
   Size:        728      35    1472

  Fragments arranged by size: 

                1472     728      35
 
ApaI G_GGCC'C

Cuts at:      0      18    1130    1531    1538    2235 
   Size:         18    1112     401       7     697

  Fragments arranged by size: 

                1112     697     401      18       7
 
ApaLI G'TGCA_C

Cuts at:      0     689    1067    2235 
   Size:        689     378    1168

  Fragments arranged by size: 

                1168     689     378
 
AseI AT'TA_AT

Cuts at:      0    2207    2235 
   Size:       2207      28
 
AvaI C'yCGr_G

Cuts at:      0     416     596     610    1237    1282    1564    1860    1954 
   Size:        416     180      14     627      45     282     296      94

Cuts at:   1954    2235 
   Size:        281

  Fragments arranged by size: 

                 627     416     296     282     281     180      94      45
                  14
 
BanI G'GyrC_C

Cuts at:      0     132     486     493     657     739     835     972    1023 
   Size:        132     354       7     164      82      96     137      51

Cuts at:   1023    1084    1147    1681    1828    2176    2235 
   Size:         61      63     534     147     348      59

  Fragments arranged by size: 

                 534     354     348     164     147     137     132      96
                  82      63      61      59      51       7
 
BanII G_rGCy'C

Cuts at:      0      18     409     617    1130    1436    1531    1538    1557 
   Size:         18     391     208     513     306      95       7      19

Cuts at:   1557    1760    2149    2235 
   Size:        203     389      86

  Fragments arranged by size: 

                 513     391     389     306     208     203      95      86
                  19      18       7
 
BbeI G_GCGC'C

Cuts at:      0     490     976    1088    1151    1832    2180    2235 
   Size:        490     486     112      63     681     348      55

  Fragments arranged by size: 

                 681     490     486     348     112      63      55
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1343    1440    2042    2235 
   Size:       1343      97     602     193

  Fragments arranged by size: 

                1343     602     193      97
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     607     641    1924    1958    2235 
   Size:        607      34    1283      34     277

  Fragments arranged by size: 

                1283     607     277      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1045    2235 
   Size:       1045    1190
 
BlpI GC'TnA_GC

Cuts at:      0     508    2235 
   Size:        508    1727
 
Bme1580I G_kGCm'C

Cuts at:      0      18     693    1028    1071    1130    1531    1538    1820 
   Size:         18     675     335      43      59     401       7     282

Cuts at:   1820    2235 
   Size:        415

  Fragments arranged by size: 

                 675     415     401     335     282      59      43      18
                   7
 
BmgBI CAC'GTC

Cuts at:      0     233    1446    2013    2235 
   Size:        233    1213     567     222

  Fragments arranged by size: 

                1213     567     233     222
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2106    2235 
   Size:       2106     129
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     636    2163    2235 
   Size:        636    1527      72

  Fragments arranged by size: 

                1527     636      72
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     938    2235 
   Size:        938    1297
 
Bpu10I CC'TnA_GC

Cuts at:      0     364    2235 
   Size:        364    1871
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286    1420    2235 
   Size:        286    1134     815

  Fragments arranged by size: 

                1134     815     286
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1282    2045    2235 
   Size:       1282     763     190

  Fragments arranged by size: 

                1282     763     190
 
BsaAI yAC'GTr

Cuts at:      0    1728    2235 
   Size:       1728     507
 
BsaHI Gr'CG_yC

Cuts at:      0     218     487     973    1052    1085    1148    1460    1646 
   Size:        218     269     486      79      33      63     312     186

Cuts at:   1646    1829    2177    2235 
   Size:        183     348      58

  Fragments arranged by size: 

                 486     348     312     269     218     186     183      79
                  63      58      33
 
BsaWI w'CCGG_w

Cuts at:      0     855    2235 
   Size:        855    1380
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     936     966    2098    2128    2235 
   Size:        936      30    1132      30     107

  Fragments arranged by size: 

                1132     936     107      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1321    1342    1378    2014    2092    2146    2235 
   Size:       1321      21      36     636      78      54      89

  Fragments arranged by size: 

                1321     636      89      78      54      36      21
 
BseYI C'CCAG_C

Cuts at:      0     697     978    1080    1455    1523    2235 
   Size:        697     281     102     375      68     712

  Fragments arranged by size: 

                 712     697     375     281     102      68
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1519    1642    2035    2235 
   Size:       1519     123     393     200

  Fragments arranged by size: 

                1519     393     200     123
 
BsiEI CG_ry'CG

Cuts at:      0    1678    2163    2235 
   Size:       1678     485      72

  Fragments arranged by size: 

                1678     485      72
 
BsiHKAI G_wGCw'C

Cuts at:      0     595     693    1071    1436    1760    2235 
   Size:        595      98     378     365     324     475

  Fragments arranged by size: 

                 595     475     378     365     324      98
 
BsiWI C'GTAC_G

Cuts at:      0     478    2235 
   Size:        478    1757
 
BsmI GAATG_Cn'

Cuts at:      0    1277    2235 
   Size:       1277     958
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1288    2235 
   Size:       1288     947
 
Bsp1286I G_dGCh'C

Cuts at:      0      18     409     595     617     693    1028    1071    1130 
   Size:         18     391     186      22      76     335      43      59

Cuts at:   1130    1436    1531    1538    1557    1760    1820    2149    2235 
   Size:        306      95       7      19     203      60     329      86

  Fragments arranged by size: 

                 391     335     329     306     203     186      95      86
                  76      60      59      43      22      19      18       7
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     362     452    2075    2235 
   Size:        362      90    1623     160

  Fragments arranged by size: 

                1623     362     160      90
 
BsrBI CCG'CTC

Cuts at:      0     866    2235 
   Size:        866    1369
 
BsrDI GCAATG_nn'

Cuts at:      0     623     817    2235 
   Size:        623     194    1418

  Fragments arranged by size: 

                1418     623     194
 
BsrFI r'CCGG_y

Cuts at:      0     470     660     855     969    2235 
   Size:        470     190     195     114    1266

  Fragments arranged by size: 

                1266     470     195     190     114
 
BssHII G'CGCG_C

Cuts at:      0     368     512     750    1519    2235 
   Size:        368     144     238     769     716

  Fragments arranged by size: 

                 769     716     368     238     144
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     430     657    2235 
   Size:        430     227    1578

  Fragments arranged by size: 

                1578     430     227
 
BstYI r'GATC_y

Cuts at:      0     824    1343    2235 
   Size:        824     519     892

  Fragments arranged by size: 

                 892     824     519
 
Bsu36I CC'TnA_GG

Cuts at:      0    1892    2235 
   Size:       1892     343
 
BtgI C'CryG_G

Cuts at:      0     266     343    1665    2235 
   Size:        266      77    1322     570

  Fragments arranged by size: 

                1322     570     266      77
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     765    1449    1748    1905    2178    2235 
   Size:        765     684     299     157     273      57

  Fragments arranged by size: 

                 765     684     299     273     157      57
 
BtsI GCAGTG_nn'

Cuts at:      0     549     745     774    1625    1630    1790    2235 
   Size:        549     196      29     851       5     160     445

  Fragments arranged by size: 

                 851     549     445     196     160      29       5
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1088    1123    2235 
   Size:       1088      35    1112

  Fragments arranged by size: 

                1112    1088      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     430    2235 
   Size:        430    1805
 
EaeI y'GGCC_r

Cuts at:      0      47     284     789     967    1265    1675    1808    2235 
   Size:         47     237     505     178     298     410     133     427

  Fragments arranged by size: 

                 505     427     410     298     237     178     133      47
            
 
EagI C'GGCC_G

Cuts at:      0    1675    2235 
   Size:       1675     560
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     904    1375    1495    1565    2235 
   Size:        904     471     120      70     670

  Fragments arranged by size: 

                 904     670     471     120      70
 
EcoICRI GAG'CTC

Cuts at:      0    1434    1758    2235 
   Size:       1434     324     477

  Fragments arranged by size: 

                1434     477     324
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     805     938    2235 
   Size:        805     133    1297

  Fragments arranged by size: 

                1297     805     133
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1267    2235 
   Size:       1267     968
 
EcoO109I rG'GnC_Cy

Cuts at:      0      15     206    1127    1173    1386    1527    1528    1896 
   Size:         15     191     921      46     213     141       1     368

Cuts at:   1896    2025    2064    2235 
   Size:        129      39     171

  Fragments arranged by size: 

                 921     368     213     191     171     141     129      46
                  39      15       1
 
FspI TGC'GCA

Cuts at:      0     262     358     457    2235 
   Size:        262      96      99    1778

  Fragments arranged by size: 

                1778     262      99      96
 
FspAI rTGC'GCAy

Cuts at:      0     262    2235 
   Size:        262    1973
 
HaeII r_GCGC'y

Cuts at:      0      37     490     976    1088    1151    1374    1708    1832 
   Size:         37     453     486     112      63     223     334     124

Cuts at:   1832    2180    2235 
   Size:        348      55

  Fragments arranged by size: 

                 486     453     348     334     223     124     112      63
                  55      37
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     153     185    1177    1209    1747    1779    2098    2130 
   Size:        153      32     992      32     538      32     319      32

Cuts at:   2130    2235 
   Size:        105

  Fragments arranged by size: 

                 992     538     319     153     105      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1650    2190    2235 
   Size:       1650     540      45

  Fragments arranged by size: 

                1650     540      45
 
KasI G'GCGC_C

Cuts at:      0     486     972    1084    1147    1828    2176    2235 
   Size:        486     486     112      63     681     348      59

  Fragments arranged by size: 

                 681     486     486     348     112      63      59
 
KpnI G_GTAC'C

Cuts at:      0    1685    2235 
   Size:       1685     550
 
MluI A'CGCG_T

Cuts at:      0    1104    1412    2235 
   Size:       1104     308     823

  Fragments arranged by size: 

                1104     823     308
 
MscI TGG'CCA

Cuts at:      0    1267    1810    2235 
   Size:       1267     543     425

  Fragments arranged by size: 

                1267     543     425
 
MslI CAynn'nnrTG

Cuts at:      0     598     710    2235 
   Size:        598     112    1525

  Fragments arranged by size: 

                1525     598     112
 
MspA1I CmG'CkG

Cuts at:      0     378    1503    1512    1603    1667    2235 
   Size:        378    1125       9      91      64     568

  Fragments arranged by size: 

                1125     568     378      91      64       9
 
NaeI GCC'GGC

Cuts at:      0     971    2235 
   Size:        971    1264
 
NarI GG'CG_CC

Cuts at:      0     487     973    1085    1148    1829    2177    2235 
   Size:        487     486     112      63     681     348      58

  Fragments arranged by size: 

                 681     487     486     348     112      63      58
 
NgoMIV G'CCGG_C

Cuts at:      0     969    2235 
   Size:        969    1266
 
PasI CC'CwG_GG

Cuts at:      0    1531    2172    2235 
   Size:       1531     641      63

  Fragments arranged by size: 

                1531     641      63
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1263    1848    2235 
   Size:       1263     585     387

  Fragments arranged by size: 

                1263     585     387
 
PmlI CAC'GTG

Cuts at:      0    1728    2235 
   Size:       1728     507
 
PpuMI rG'GwC_Cy

Cuts at:      0    1386    2064    2235 
   Size:       1386     678     171

  Fragments arranged by size: 

                1386     678     171
 
PspOMI G'GGCC_C

Cuts at:      0      14    1126    1527    1534    2235 
   Size:         14    1112     401       7     701

  Fragments arranged by size: 

                1112     701     401      14       7
 
PspXI vC'TCGA_Gb

Cuts at:      0    1954    2235 
   Size:       1954     281
 
PstI C_TGCA'G

Cuts at:      0     377     578     653    2072    2235 
   Size:        377     201      75    1419     163

  Fragments arranged by size: 

                1419     377     201     163      75
 
PvuI CG_AT'CG

Cuts at:      0    2163    2235 
   Size:       2163      72
 
PvuII CAG'CTG

Cuts at:      0     378    1503    2235 
   Size:        378    1125     732

  Fragments arranged by size: 

                1125     732     378
 
SacI G_AGCT'C

Cuts at:      0    1436    1760    2235 
   Size:       1436     324     475

  Fragments arranged by size: 

                1436     475     324
 
SacII CC_GC'GG

Cuts at:      0    1668    2235 
   Size:       1668     567
 
SanDI GG'GwC_CC

Cuts at:      0    1386    2235 
   Size:       1386     849
 
SfcI C'TryA_G

Cuts at:      0     373     574     649    2068    2217    2235 
   Size:        373     201      75    1419     149      18

  Fragments arranged by size: 

                1419     373     201     149      75      18
 
SfoI GGC'GCC

Cuts at:      0     488     974    1086    1149    1830    2178    2235 
   Size:        488     486     112      63     681     348      57

  Fragments arranged by size: 

                 681     488     486     348     112      63      57
 
SmaI CCC'GGG

Cuts at:      0     418     598    1566    1862    2235 
   Size:        418     180     968     296     373

  Fragments arranged by size: 

                 968     418     373     296     180
 
SmlI C'TyrA_G

Cuts at:      0     301    1435    1954    2235 
   Size:        301    1134     519     281

  Fragments arranged by size: 

                1134     519     301     281
 
StuI AGG'CCT

Cuts at:      0     580    1262    2235 
   Size:        580     682     973

  Fragments arranged by size: 

                 973     682     580
 
StyI C'CwwG_G

Cuts at:      0    1040    1202    1257    1268    2117    2235 
   Size:       1040     162      55      11     849     118

  Fragments arranged by size: 

                1040     849     162     118      55      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1306    2235 
   Size:       1306     929
 
TatI w'GTAC_w

Cuts at:      0    2153    2235 
   Size:       2153      82
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     977    1009    2235 
   Size:        977      32    1226

  Fragments arranged by size: 

                1226     977      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     962    2206    2235 
   Size:        962    1244      29

  Fragments arranged by size: 

                1244     962      29
 
XhoI C'TCGA_G

Cuts at:      0    1954    2235 
   Size:       1954     281
 
XmaI C'CCGG_G

Cuts at:      0     416     596    1564    1860    2235 
   Size:        416     180     968     296     375

  Fragments arranged by size: 

                 968     416     375     296     180
 
ZraI GAC'GTC

Cuts at:      0     219    2235 
   Size:        219    2016
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     ApaI    ApaLI     AseI     AvaI     BanI    BanII
     BbeI     BbsI     BcgI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BssHII   BstAPI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     EaeI
     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI    FspAI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     PasI    PflMI     PmlI    PpuMI
   PspOMI    PspXI     PstI     PvuI    PvuII     SacI    SacII    SanDI
     SfcI     SfoI     SmaI     SmlI     StuI     StyI    TaqII     TatI
     TstI     XcmI     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AclI    AflII     AloI    AlwNI     ApoI     AscI    AsiSI
    AvrII     BaeI    BamHI    BbvCI    BciVI     BclI    BfrBI    BglII
     BmtI    BsaBI    BspEI    BspHI    BsrGI    BssSI    BstBI   BstEII
    BstXI  BstZ17I     ClaI     DraI     DrdI     EarI    EcoRI    EcoRV
     FalI     FseI  HindIII     HpaI     MfeI     MmeI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI     PfoI
     PmeI     PpiI    PshAI     PsiI     PsrI    RsrII     SalI     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XmnI
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