Restriction map for pF1KSDA1441
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AcsI        AgeI        AhdI        Alw44I      ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AvrII       BamHI       BclI        BglII       
BlnI        BsaI        BseAI       BsiEI       BsiWI       
Bsp1407I    BspDI       BspEI       BsrGI       BssHII      
Bst1107I    BstBI       BstUI       BstZ17I     ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco52I      EcoRI       FbaI        FseI        
HgaI        HincII      HindII      HindIII     HpaI        
KpnI        KspI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NdeI        NgoMIV      
NotI        NruI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SspBI       SspI        SwaI        XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII        2352  GACGTC               GACGT^C
AflII         982  CTTAAG               C^TTAAG
AflIII       3129  ACATGT               A^C[GA][CT]GT
Aor51HI      2996  AGCGCT               AGC^GCT
Asp700I      2970  GAAAAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1779  GTGCTC               G[AT]GC[AT]^C
AviII        1670  TGCGCA               TGC^GCA
BalI         1208  TGGCCA               TGG^CCA
BbeI         3026  GGCGCC               GGCGC^C
BbrPI        1745  CACGTG               CAC^GTG
BciVI        2287  GGATAC               GTATCC(6/5)
BfrI          982  CTTAAG               C^TTAAG
BlpI          302  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      302  GCTCAGC              GC^T[ACGT]AGC
BsaBI        1054  GATCCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        3341  ACCGGA               [AT]^CCGG[AT]
BsiHKAI      1779  GTGCTC               G[AT]GC[AT]^C
BsmI          365  GAATGC               GAATGC(1/-1)
BspLU11I     3129  ACATGT               A^CATGT
BsrBI        1966  CCGCTC               CCGCTC(-3/-3)
BsrFI        1591  ACCGGC               [GA]^CCGG[CT]
BssSI        3046  CACGAG               CACGAG(-5/-1)
BstEII       1680  GGTCACC              G^GT[ACGT]ACC
BstPI        1680  GGTCACC              G^GT[ACGT]ACC
CelII         302  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1591  ACCGGC               [GA]^CCGG[CT]
DraIII        264  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     2996  AGCGCT               AGC^GCT
EcoO65I      1680  GGTCACC              G^GT[ACGT]ACC
EcoRV        1467  GATATC               GAT^ATC
EcoT22I      2176  ATGCAT               ATGCA^T
FspI         1670  TGCGCA               TGC^GCA
KasI         3026  GGCGCC               G^GCGCC
MamI         1054  GATCCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1208  TGGCCA               TGG^CCA
MscI         1208  TGGCCA               TGG^CCA
NarI         3026  GGCGCC               GG^CGCC
NheI         1102  GCTAGC               G^CTAGC
NsiI         2176  ATGCAT               ATGCA^T
PmaCI        1745  CACGTG               CAC^GTG
PmlI         1745  CACGTG               CAC^GTG
SapI         1635  GAAGAGC              GCTCTTC(1/4)
SbfI         3071  CCTGCAGG             CCTGCA^GG
ScaI         1228  AGTACT               AGT^ACT
SexAI         132  ACCAGGT              A^CC[AT]GGT
SfiI          347  GGCCTGTGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         3026  GGCGCC               GGC^GCC
Sse8387I     3071  CCTGCAGG             CCTGCA^GG
Tsp509I      2236  AATT                 ^AATT
XmnI         2970  GAAAAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcyI         2352  GACGTC               G[GA]^CG[CT]C
             3026  GGCGCC              
ApaI          317  GGGCCC               GGGCC^C
             3161  GGGCCC              
AspI         1476  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2497  GACACAGTC           
BsaAI        1745  CACGTG               [CT]AC^GT[GA]
             2932  TACGTG              
BsaHI        2352  GACGTC               G[GA]^CG[CT]C
             3026  GGCGCC              
BspHI        1793  TCATGA               T^CATGA
             3371  TCATGA              
BsrDI         947  GCAATG               GCAATG(2/0)
             2111  GCAATG              
Hin1I        2352  GACGTC               G[GA]^CG[CT]C
             3026  GGCGCC              
MslI         1236  CATGCTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1741  CATGCACGTG          
PflFI        1476  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2497  GACACAGTC           
PflMI         766  CCAGTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1815  CCAGATGGTGG         
PpuMI         695  AGGTCCT              [GA]G^G[AT]CC[CT]
             1043  AGGTCCC             
PvuII        1562  CAGCTG               CAG^CTG
             2301  CAGCTG              
RcaI         1793  TCATGA               T^CATGA
             3371  TCATGA              
TfiI          148  GAATC                G^A[AT]TC
             2184  GAATC               
Tsp45I       1681  GTCAC                ^GT[GC]AC
             2903  GTCAC               
Tth111I      1476  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2497  GACACAGTC           
Van91I        766  CCAGTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1815  CCAGATGGTGG         
XcmI         2300  CCAGCTGTTCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2384  CCATCTGCCCCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AocI        AvaI        AvaII       BanI        BanII       
BbsI        BbvCI       BbvI        BcgI        BcnI        
BfaI        BglI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstXI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SphI        StuI        
StyI        TaqI        Tru9I       TseI        TspRI       
TthHB8I     XhoII       
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