Restriction map for pF1KSDA1441
 (Linear) (Six-base) MAPSORT of: KIAA1441.seq  Check: 8602  from: 1  to: 3711



KIAA1441.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1074    1693    3711 
   Size:       1074     619    2018

  Fragments arranged by size: 

                2018    1074     619
 
AatII G_ACGT'C

Cuts at:      0    2356    3711 
   Size:       2356    1355
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     173    2142    2759    3087    3711 
   Size:        173    1969     617     328     624

  Fragments arranged by size: 

                1969     624     617     328     173
 
AfeI AGC'GCT

Cuts at:      0    2998    3711 
   Size:       2998     713
 
AflII C'TTAA_G

Cuts at:      0     982    3711 
   Size:        982    2729
 
AflIII A'CryG_T

Cuts at:      0    3129    3711 
   Size:       3129     582
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1216    1543    2242    3711 
   Size:       1216     327     699    1469

  Fragments arranged by size: 

                1469    1216     699     327
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     383     415    3711 
   Size:        383      32    3296

  Fragments arranged by size: 

                3296     383      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     172     772    1057    1438    1945    2749    3711 
   Size:        172     600     285     381     507     804     962

  Fragments arranged by size: 

                 962     804     600     507     381     285     172
 
ApaI G_GGCC'C

Cuts at:      0     321    3165    3711 
   Size:        321    2844     546

  Fragments arranged by size: 

                2844     546     321
 
AvaI C'yCGr_G

Cuts at:      0     274     554    3194    3711 
   Size:        274     280    2640     517

  Fragments arranged by size: 

                2640     517     280     274
 
BanI G'GyrC_C

Cuts at:      0     439     777     803    1332    1399    2294    2686    3026 
   Size:        439     338      26     529      67     895     392     340

Cuts at:   3026    3397    3711 
   Size:        371     314

  Fragments arranged by size: 

                 895     529     439     392     371     340     338     314
                  67      26
 
BanII G_rGCy'C

Cuts at:      0     321     388     567     814    1558    1769    2810    3165 
   Size:        321      67     179     247     744     211    1041     355

Cuts at:   3165    3216    3687    3711 
   Size:         51     471      24

  Fragments arranged by size: 

                1041     744     471     355     321     247     211     179
                  67      51      24
 
BbeI G_GCGC'C

Cuts at:      0    3030    3711 
   Size:       3030     681
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      19     161     881    1704    2496    2759    3236    3711 
   Size:         19     142     720     823     792     263     477     475

  Fragments arranged by size: 

                 823     792     720     477     475     263     142      19
            
 
BbvCI CC'TCA_GC

Cuts at:      0    1079    1097    1565    1949    3711 
   Size:       1079      18     468     384    1762

  Fragments arranged by size: 

                1762    1079     468     384      18
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1656    1690    2167    2201    2257    2291    3711 
   Size:       1656      34     477      34      56      34    1420

  Fragments arranged by size: 

                1656    1420     477      56      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2281    3711 
   Size:       2281    1430
 
BfrBI ATG'CAT

Cuts at:      0    2178    3711 
   Size:       2178    1533
 
BglI GCCn_nnn'nGGC

Cuts at:      0     354    1075    1093    1255    2101    3711 
   Size:        354     721      18     162     846    1610

  Fragments arranged by size: 

                1610     846     721     354     162      18
 
BlpI GC'TnA_GC

Cuts at:      0     303    3711 
   Size:        303    3408
 
Bme1580I G_kGCm'C

Cuts at:      0     321     442     782     806     836    1337    2122    2299 
   Size:        321     121     340      24      30     501     785     177

Cuts at:   2299    2383    2421    2581    2691    3165    3402    3507    3711 
   Size:         84      38     160     110     474     237     105     204

  Fragments arranged by size: 

                 785     501     474     340     321     237     204     177
                 160     121     110     105      84      38      30      24
            
 
BmgBI CAC'GTC

Cuts at:      0    2271    3711 
   Size:       2271    1440
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     425     760    1388    1448    1473    3159    3711 
   Size:        425     335     628      60      25    1686     552

  Fragments arranged by size: 

                1686     628     552     425     335      60      25
 
BmtI G_CTAG'C

Cuts at:      0    1106    3711 
   Size:       1106    2605
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     209     317     705     782     948    1627    2047 
   Size:        131      78     108     388      77     166     679     420

Cuts at:   2047    2900    3338    3608    3711 
   Size:        853     438     270     103

  Fragments arranged by size: 

                 853     679     438     420     388     270     166     131
                 108     103      78      77
 
Bpu10I CC'TnA_GC

Cuts at:      0     503     714    1079    1097    1552    1565    1949    2804 
   Size:        503     211     365      18     455      13     384     855

Cuts at:   2804    2918    3448    3711 
   Size:        114     530     263

  Fragments arranged by size: 

                 855     530     503     455     384     365     263     211
                 114      18      13
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2614    2842    3628    3711 
   Size:       2614     228     786      83

  Fragments arranged by size: 

                2614     786     228      83
 
BsaAI yAC'GTr

Cuts at:      0    1747    2934    3711 
   Size:       1747    1187     777

  Fragments arranged by size: 

                1747    1187     777
 
BsaBI GATnn'nnATC

Cuts at:      0    1058    3711 
   Size:       1058    2653
 
BsaHI Gr'CG_yC

Cuts at:      0    2353    3027    3711 
   Size:       2353     674     684

  Fragments arranged by size: 

                2353     684     674
 
BsaWI w'CCGG_w

Cuts at:      0    3341    3711 
   Size:       3341     370
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     383     413     561     591    2419    2449    3711 
   Size:        383      30     148      30    1828      30    1262

  Fragments arranged by size: 

                1828    1262     383     148      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     416     960    1914    2512    2517    2674    2731    2773 
   Size:        416     544     954     598       5     157      57      42

Cuts at:   2773    2797    3277    3711 
   Size:         24     480     434

  Fragments arranged by size: 

                 954     598     544     480     434     416     157      57
                  42      24       5
 
BseYI C'CCAG_C

Cuts at:      0     175     328     772    1371    1570    2299    2438    2446 
   Size:        175     153     444     599     199     729     139       8

Cuts at:   2446    2555    2668    2794    3016    3401    3513    3711 
   Size:        109     113     126     222     385     112     198

  Fragments arranged by size: 

                 729     599     444     385     222     199     198     175
                 153     139     126     113     112     109       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     524    1746    2251    3711 
   Size:        524    1222     505    1460

  Fragments arranged by size: 

                1460    1222     524     505
 
BsiHKAI G_wGCw'C

Cuts at:      0    1783    3711 
   Size:       1783    1928
 
BsmI GAATG_Cn'

Cuts at:      0     371    3711 
   Size:        371    3340
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     305    2277    3474    3711 
   Size:        305    1972    1197     237

  Fragments arranged by size: 

                1972    1197     305     237
 
Bsp1286I G_dGCh'C

Cuts at:      0     321     388     442     567     782     806     814     836 
   Size:        321      67      54     125     215      24       8      22

Cuts at:    836    1337    1558    1769    1783    2122    2299    2383    2421 
   Size:        501     221     211      14     339     177      84      38

Cuts at:   2421    2581    2691    2810    3165    3216    3402    3507    3687 
   Size:        160     110     119     355      51     186     105     180

Cuts at:   3687    3711 
   Size:         24

  Fragments arranged by size: 

                 501     355     339     321     221     215     211     186
                 180     177     160     125     119     110     105      84
                  67      54      51      38      24      24      22      14
                   8
 
BspHI T'CATG_A

Cuts at:      0    1793    3371    3711 
   Size:       1793    1578     340

  Fragments arranged by size: 

                1793    1578     340
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1074    1535    1693    2134    3065    3711 
   Size:       1074     461     158     441     931     646

  Fragments arranged by size: 

                1074     931     646     461     441     158
 
BsrBI CCG'CTC

Cuts at:      0    1968    3711 
   Size:       1968    1743
 
BsrDI GCAATG_nn'

Cuts at:      0     954    2118    3711 
   Size:        954    1164    1593

  Fragments arranged by size: 

                1593    1164     954
 
BsrFI r'CCGG_y

Cuts at:      0    1591    3711 
   Size:       1591    2120
 
BssSI C'ACGA_G

Cuts at:      0    3046    3711 
   Size:       3046     665
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     172    1694    2117    2550    2749    3080    3150    3623 
   Size:        172    1522     423     433     199     331      70     473

Cuts at:   3623    3711 
   Size:         88

  Fragments arranged by size: 

                1522     473     433     423     331     199     172      88
                  70
 
BstEII G'GTnAC_C

Cuts at:      0    1680    3711 
   Size:       1680    2031
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     328     613    1242    1491    2647    3172    3507    3711 
   Size:        328     285     629     249    1156     525     335     204

  Fragments arranged by size: 

                1156     629     525     335     328     285     249     204
            
 
BstYI r'GATC_y

Cuts at:      0     522    1053    1508    3313    3711 
   Size:        522     531     455    1805     398

  Fragments arranged by size: 

                1805     531     522     455     398
 
Bsu36I CC'TnA_GG

Cuts at:      0     728    1349    2714    3022    3711 
   Size:        728     621    1365     308     689

  Fragments arranged by size: 

                1365     728     689     621     308
 
BtgI C'CryG_G

Cuts at:      0     213     734    2393    3324    3711 
   Size:        213     521    1659     931     387

  Fragments arranged by size: 

                1659     931     521     387     213
 
BtsI GCAGTG_nn'

Cuts at:      0    1692    1783    2358    3264    3412    3711 
   Size:       1692      91     575     906     148     299

  Fragments arranged by size: 

                1692     906     575     299     148      91
 
DraIII CAC_nnn'GTG

Cuts at:      0     269    3711 
   Size:        269    3442
 
EaeI y'GGCC_r

Cuts at:      0    1208    1994    3174    3711 
   Size:       1208     786    1180     537

  Fragments arranged by size: 

                1208    1180     786     537
 
EarI CTCTTCn'nnn_

Cuts at:      0     594    1630    2774    3472    3524    3711 
   Size:        594    1036    1144     698      52     187

  Fragments arranged by size: 

                1144    1036     698     594     187      52
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3000    3320    3711 
   Size:       3000     320     391

  Fragments arranged by size: 

                3000     391     320
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     173     209     317     705     782     948    1627 
   Size:        131      42      36     108     388      77     166     679

Cuts at:   1627    2047    2142    2759    2900    3087    3338    3608    3711 
   Size:        420      95     617     141     187     251     270     103

  Fragments arranged by size: 

                 679     617     420     388     270     251     187     166
                 141     131     108     103      95      77      42      36
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     506    1348    1492    3711 
   Size:        506     842     144    2219

  Fragments arranged by size: 

                2219     842     506     144
 
EcoO109I rG'GnC_Cy

Cuts at:      0     106     288     317     346     355     656     696    1044 
   Size:        106     182      29      29       9     301      40     348

Cuts at:   1044    1085    1953    2875    3161    3534    3711 
   Size:         41     868     922     286     373     177

  Fragments arranged by size: 

                 922     868     373     348     301     286     182     177
                 106      41      40      29      29       9
 
EcoRV GAT'ATC

Cuts at:      0    1469    3711 
   Size:       1469    2242
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3669    3711 
   Size:       3669      42
 
FspI TGC'GCA

Cuts at:      0    1672    3711 
   Size:       1672    2039
 
FspAI rTGC'GCAy

Cuts at:      0    1672    3711 
   Size:       1672    2039
 
HaeII r_GCGC'y

Cuts at:      0    2165    2655    3000    3030    3711 
   Size:       2165     490     345      30     681

  Fragments arranged by size: 

                2165     681     490     345      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     561     593    1023    1055    1609    1641    2749    2781 
   Size:        561      32     430      32     554      32    1108      32

Cuts at:   2781    3711 
   Size:        930

  Fragments arranged by size: 

                1108     930     561     554     430      32      32      32
                  32
 
KasI G'GCGC_C

Cuts at:      0    3026    3711 
   Size:       3026     685
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1642    3208    3711 
   Size:       1642    1566     503

  Fragments arranged by size: 

                1642    1566     503
 
MscI TGG'CCA

Cuts at:      0    1210    3711 
   Size:       1210    2501
 
MslI CAynn'nnrTG

Cuts at:      0    1240    1745    3711 
   Size:       1240     505    1966

  Fragments arranged by size: 

                1966    1240     505
 
MspA1I CmG'CkG

Cuts at:      0     528    1564    1601    1845    2303    3711 
   Size:        528    1036      37     244     458    1408

  Fragments arranged by size: 

                1408    1036     528     458     244      37
 
NarI GG'CG_CC

Cuts at:      0    3027    3711 
   Size:       3027     684
 
NcoI C'CATG_G

Cuts at:      0     213    2393    3324    3711 
   Size:        213    2180     931     387

  Fragments arranged by size: 

                2180     931     387     213
 
NheI G'CTAG_C

Cuts at:      0    1102    3711 
   Size:       1102    2609
 
NsiI A_TGCA'T

Cuts at:      0    2180    3711 
   Size:       2180    1531
 
NspI r_CATG'y

Cuts at:      0     627    1671    1744    2015    2178    3133    3711 
   Size:        627    1044      73     271     163     955     578

  Fragments arranged by size: 

                1044     955     627     578     271     163      73
 
PasI CC'CwG_GG

Cuts at:      0     118    2098    3216    3299    3507    3711 
   Size:        118    1980    1118      83     208     204

  Fragments arranged by size: 

                1980    1118     208     204     118      83
 
PciI A'CATG_T

Cuts at:      0    3129    3711 
   Size:       3129     582
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     772    1821    3711 
   Size:        772    1049    1890

  Fragments arranged by size: 

                1890    1049     772
 
PfoI T'CCnGG_A

Cuts at:      0    2252    3711 
   Size:       2252    1459
 
PmlI CAC'GTG

Cuts at:      0    1747    3711 
   Size:       1747    1964
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     383     415    3353    3385    3711 
   Size:        383      32    2938      32     326

  Fragments arranged by size: 

                2938     383     326      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     696    1044    3711 
   Size:        696     348    2667

  Fragments arranged by size: 

                2667     696     348
 
PspOMI G'GGCC_C

Cuts at:      0     317    3161    3711 
   Size:        317    2844     550

  Fragments arranged by size: 

                2844     550     317
 
PstI C_TGCA'G

Cuts at:      0     679     936    2155    3076    3711 
   Size:        679     257    1219     921     635

  Fragments arranged by size: 

                1219     921     679     635     257
 
PvuII CAG'CTG

Cuts at:      0    1564    2303    3711 
   Size:       1564     739    1408

  Fragments arranged by size: 

                1564    1408     739
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1630    3711 
   Size:       1630    2081
 
SbfI CC_TGCA'GG

Cuts at:      0    3076    3711 
   Size:       3076     635
 
ScaI AGT'ACT

Cuts at:      0    1230    3711 
   Size:       1230    2481
 
SexAI A'CCwGG_T

Cuts at:      0     132    3711 
   Size:        132    3579
 
SfcI C'TryA_G

Cuts at:      0     239     675     932    1539    1802    1853    1937    2151 
   Size:        239     436     257     607     263      51      84     214

Cuts at:   2151    3072    3711 
   Size:        921     639

  Fragments arranged by size: 

                 921     639     607     436     263     257     239     214
                  84      51
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     354    3711 
   Size:        354    3357
 
SfoI GGC'GCC

Cuts at:      0    3028    3711 
   Size:       3028     683
 
SmlI C'TyrA_G

Cuts at:      0     982    2629    2857    3643    3711 
   Size:        982    1647     228     786      68

  Fragments arranged by size: 

                1647     982     786     228      68
 
SphI G_CATG'C

Cuts at:      0     627    1671    1744    2178    3711 
   Size:        627    1044      73     434    1533

  Fragments arranged by size: 

                1533    1044     627     434      73
 
StuI AGG'CCT

Cuts at:      0     205     978    1328    1498    1800    2260    2855    3711 
   Size:        205     773     350     170     302     460     595     856

  Fragments arranged by size: 

                 856     773     595     460     350     302     205     170
            
 
StyI C'CwwG_G

Cuts at:      0     213     476    1073    1895    2393    3188    3324    3711 
   Size:        213     263     597     822     498     795     136     387

  Fragments arranged by size: 

                 822     795     597     498     387     263     213     136
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1159    2653    3711 
   Size:       1159    1494    1058

  Fragments arranged by size: 

                1494    1159    1058
 
TatI w'GTAC_w

Cuts at:      0    1228    1447    3711 
   Size:       1228     219    2264

  Fragments arranged by size: 

                2264    1228     219
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     255     287    1376    1408    2419    2451    3711 
   Size:        255      32    1089      32    1011      32    1260

  Fragments arranged by size: 

                1260    1089    1011     255      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1479    2500    3711 
   Size:       1479    1021    1211

  Fragments arranged by size: 

                1479    1211    1021
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2307    2391    3711 
   Size:       2307      84    1320

  Fragments arranged by size: 

                2307    1320      84
 
XmnI GAAnn'nnTTC

Cuts at:      0    2974    3711 
   Size:       2974     737
 
ZraI GAC'GTC

Cuts at:      0    2354    3711 
   Size:       2354    1357
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI    AflII   AflIII     AlfI     AloI
    AlwNI     ApaI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII     EaeI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FspI    FspAI
    HaeII    Hin4I     KasI     MmeI     MscI     MslI   MspA1I     NarI
     NcoI     NheI     NsiI     NspI     PasI     PciI    PflMI     PfoI
     PmlI     PpiI    PpuMI   PspOMI     PstI    PvuII     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SmlI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AgeI     AhdI     AleI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BclI    BglII
     BplI     BsaI    BsiEI    BsiWI    BspEI    BsrGI   BssHII    BstBI
  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI  EcoICRI
    EcoRI     FseI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     NaeI     NdeI   NgoMIV     NotI     NruI     PacI     PmeI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     SwaI     XbaI     XhoI     XmaI
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