Restriction map for pF1KSDA1438
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbrPI       BciVI       BclI        BfrI        BglII       
BlnI        BsaAI       BsaBI       BsaWI       BseAI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BspMI       BsrDI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MunI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        ScaI        
SexAI       SfuI        SgrAI       SnaBI       SpeI        
SspBI       SspI        SwaI        Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcsI         2279  AAATTT               [GA]^AATT[CT]
AocI         1930  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      1376  AGCGCT               AGC^GCT
ApoI         2279  AAATTT               [GA]^AATT[CT]
Asp700I      1734  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI          961  GGCGCC               GGCGC^C
BbsI         2337  GAAGAC               GAAGAC(2/6)
BcgI          375  CGAGCAGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI        2337  GAAGAC               GAAGAC(2/6)
BsaI          139  GAGACC               GGTCTC(1/5)
BsiEI          97  CGGCCG               CG[GA][CT]^CG
BsmI         2022  GAATGC               GAATGC(1/-1)
BspHI          30  TCATGA               T^CATGA
BssSI        1489  CTCGTG               CACGAG(-5/-1)
BstAPI       2121  GCACCCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       1930  CCTCAGG              CC^T[ACGT]AGG
EagI           97  CGGCCG               C^GGCCG
EclXI          97  CGGCCG               C^GGCCG
Eco47III     1376  AGCGCT               AGC^GCT
Eco52I         97  CGGCCG               C^GGCCG
Eco81I       1930  CCTCAGG              CC^T[ACGT]AGG
KasI          961  GGCGCC               G^GCGCC
MaeII         987  ACGT                 A^CGT
MseI          623  TTAA                 T^TAA
NaeI         1029  GCCGGC               GCC^GGC
NarI          961  GGCGCC               GG^CGCC
NgoMIV       1029  GCCGGC               G^CCGGC
NspI         1616  GCATGC               [GA]CATG^[CT]
PflMI        2235  CCAGCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI           30  TCATGA               T^CATGA
SacI         2386  GAGCTC               GAGCT^C
SbfI         1464  CCTGCAGG             CCTGCA^GG
SfiI         1299  GGCCACGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          961  GGCGCC               GGC^GCC
SphI         1616  GCATGC               GCATG^C
Sse8387I     1464  CCTGCAGG             CCTGCA^GG
Tru9I         623  TTAA                 T^TAA
Van91I       2235  CCAGCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         1734  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AccII        1561  CGCG                 CG^CG
             1613  CGCG                
AcyI          961  GGCGCC               G[GA]^CG[CT]C
             2435  GACGCC              
BbvCI        2712  CCTCAGC              CCTCAGC(-5/-2)
             1285  GCTGAGG             
BsaHI         961  GGCGCC               G[GA]^CG[CT]C
             2435  GACGCC              
BsgI         1198  CTGCAC               GTGCAG(16/14)
             2773  CTGCAC              
BsrFI        1029  GCCGGC               [GA]^CCGG[CT]
             1899  GCCGGT              
BstUI        1561  CGCG                 CG^CG
             1613  CGCG                
Cfr10I       1029  GCCGGC               [GA]^CCGG[CT]
             1899  GCCGGT              
Hin1I         961  GGCGCC               G[GA]^CG[CT]C
             2435  GACGCC              
MvnI         1561  CGCG                 CG^CG
             1613  CGCG                
SapI          335  GCTCTTC              GCTCTTC(1/4)
              801  GCTCTTC             
Tsp509I      2280  AATT                 ^AATT
             2594  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
ApaI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrBI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        SmlI        StuI        
StyI        TaqI        TfiI        TseI        Tsp45I      
TspRI       TthHB8I     XcmI        XhoII       XmaCI       
XmaI        
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