Restriction map for pF1KSDA1438
 (Linear) (Six-base) MAPSORT of: KIAA1438.seq  Check: 2145  from: 1  to: 2793



KIAA1438.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     202     304     706    1084    1096    2182    2793 
   Size:        196       6     102     402     378      12    1086     611

  Fragments arranged by size: 

                1086     611     402     378     196     102      12       6
            
 
AfeI AGC'GCT

Cuts at:      0    1378    2793 
   Size:       1378    1415
 
AleI CACnn'nnGTG

Cuts at:      0    1720    2793 
   Size:       1720    1073
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2485    2793 
   Size:       2485     308
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      75     107    1726    1758    2793 
   Size:         75      32    1619      32    1035

  Fragments arranged by size: 

                1619    1035      75      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1074    1578    1638    1770    2092    2541    2793 
   Size:       1074     504      60     132     322     449     252

  Fragments arranged by size: 

                1074     504     449     322     252     132      60
 
ApaI G_GGCC'C

Cuts at:      0     974    1507    1777    1853    1939    2793 
   Size:        974     533     270      76      86     854

  Fragments arranged by size: 

                 974     854     533     270      86      76
 
ApoI r'AATT_y

Cuts at:      0    2279    2793 
   Size:       2279     514
 
AvaI C'yCGr_G

Cuts at:      0     373     428     957    1098    1414    1508    1845    1894 
   Size:        373      55     529     141     316      94     337      49

Cuts at:   1894    2793 
   Size:        899

  Fragments arranged by size: 

                 899     529     373     337     316     141      94      55
                  49
 
BanI G'GyrC_C

Cuts at:      0     753     961    1102    1714    2793 
   Size:        753     208     141     612    1079

  Fragments arranged by size: 

                1079     753     612     208     141
 
BanII G_rGCy'C

Cuts at:      0     974    1026    1170    1345    1507    1534    1683    1777 
   Size:        974      52     144     175     162      27     149      94

Cuts at:   1777    1853    1895    1939    2056    2390    2507    2615    2793 
   Size:         76      42      44     117     334     117     108     178

  Fragments arranged by size: 

                 974     334     178     175     162     149     144     117
                 117     108      94      76      52      44      42      27
            
 
BbeI G_GCGC'C

Cuts at:      0     965    2793 
   Size:        965    1828
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2344    2793 
   Size:       2344     449
 
BbvCI CC'TCA_GC

Cuts at:      0    1286    2713    2793 
   Size:       1286    1427      80

  Fragments arranged by size: 

                1427    1286      80
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     364     398    2793 
   Size:        364      34    2395

  Fragments arranged by size: 

                2395     364      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     978    1030    1174    1264    1306    2793 
   Size:        978      52     144      90      42    1487

  Fragments arranged by size: 

                1487     978     144      90      52      42
 
BlpI GC'TnA_GC

Cuts at:      0     149    1243    1387    1756    2384    2542    2698    2793 
   Size:        149    1094     144     369     628     158     156      95

  Fragments arranged by size: 

                1094     628     369     158     156     149     144      95
            
 
Bme1580I G_kGCm'C

Cuts at:      0     756     974    1105    1507    1777    1853    1939    2793 
   Size:        756     218     131     402     270      76      86     854

  Fragments arranged by size: 

                 854     756     402     270     218     131      86      76
            
 
BmgBI CAC'GTC

Cuts at:      0     988    2793 
   Size:        988    1805
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     913    1908    2787    2793 
   Size:        913     995     879       6

  Fragments arranged by size: 

                 995     913     879       6
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     442     793     936    1265    1684    1728    2069 
   Size:        145     297     351     143     329     419      44     341

Cuts at:   2069    2137    2396    2461    2473    2692    2793 
   Size:         68     259      65      12     219     101

  Fragments arranged by size: 

                 419     351     341     329     297     259     219     145
                 143     101      68      65      44      12
 
Bpu10I CC'TnA_GC

Cuts at:      0    1286    1528    1889    2609    2713    2793 
   Size:       1286     242     361     720     104      80

  Fragments arranged by size: 

                1286     720     361     242     104      80
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     691    1636    2214    2793 
   Size:        691     945     578     579

  Fragments arranged by size: 

                 945     691     579     578
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     133    2793 
   Size:        133    2660
 
BsaHI Gr'CG_yC

Cuts at:      0     962    2436    2793 
   Size:        962    1474     357

  Fragments arranged by size: 

                1474     962     357
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1063    1093    1726    1756    2411    2441    2665    2695 
   Size:       1063      30     633      30     655      30     224      30

Cuts at:   2695    2793 
   Size:         98

  Fragments arranged by size: 

                1063     655     633     224      98      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     530    1513    2399    2793 
   Size:        530     983     886     394

  Fragments arranged by size: 

                 983     886     530     394
 
BseYI C'CCAG_C

Cuts at:      0     568    1230    1682    1795    1809    1939    2057    2072 
   Size:        568     662     452     113      14     130     118      15

Cuts at:   2072    2614    2728    2793 
   Size:        542     114      65

  Fragments arranged by size: 

                 662     568     542     452     130     118     114     113
                  65      15      14
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1183    2758    2793 
   Size:       1183    1575      35

  Fragments arranged by size: 

                1575    1183      35
 
BsiEI CG_ry'CG

Cuts at:      0     100    2793 
   Size:        100    2693
 
BsiHKAI G_wGCw'C

Cuts at:      0    1249    2123    2390    2793 
   Size:       1249     874     267     403

  Fragments arranged by size: 

                1249     874     403     267
 
BsmI GAATG_Cn'

Cuts at:      0    2028    2793 
   Size:       2028     765
 
Bsp1286I G_dGCh'C

Cuts at:      0     756     974    1026    1105    1170    1249    1345    1507 
   Size:        756     218      52      79      65      79      96     162

Cuts at:   1507    1534    1683    1777    1853    1895    1939    2056    2123 
   Size:         27     149      94      76      42      44     117      67

Cuts at:   2123    2390    2507    2615    2793 
   Size:        267     117     108     178

  Fragments arranged by size: 

                 756     267     218     178     162     149     117     117
                 108      96      94      79      79      76      67      65
                  52      44      42      27
 
BspHI T'CATG_A

Cuts at:      0      30    2793 
   Size:         30    2763
 
BsrBI CCG'CTC

Cuts at:      0      54    1647    1780    2273    2793 
   Size:         54    1593     133     493     520

  Fragments arranged by size: 

                1593     520     493     133      54
 
BsrFI r'CCGG_y

Cuts at:      0    1029    1899    2793 
   Size:       1029     870     894

  Fragments arranged by size: 

                1029     894     870
 
BssSI C'ACGA_G

Cuts at:      0    1489    2793 
   Size:       1489    1304
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2127    2793 
   Size:       2127     666
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     246     300    2631    2793 
   Size:        246      54    2331     162

  Fragments arranged by size: 

                2331     246     162      54
 
BstYI r'GATC_y

Cuts at:      0     782     818    1484    2793 
   Size:        782      36     666    1309

  Fragments arranged by size: 

                1309     782     666      36
 
Bsu36I CC'TnA_GG

Cuts at:      0    1931    2793 
   Size:       1931     862
 
BtgI C'CryG_G

Cuts at:      0     239     761    1301    1859    2579    2624    2793 
   Size:        239     522     540     558     720      45     169

  Fragments arranged by size: 

                 720     558     540     522     239     169      45
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     374    1412    2793 
   Size:        374    1038    1381

  Fragments arranged by size: 

                1381    1038     374
 
BtsI GCAGTG_nn'

Cuts at:      0     442     596    1087    1320    1381    1477    2125    2549 
   Size:        442     154     491     233      61      96     648     424

Cuts at:   2549    2793 
   Size:        244

  Fragments arranged by size: 

                 648     491     442     424     244     233     154      96
                  61
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     459     494    2793 
   Size:        459      35    2299

  Fragments arranged by size: 

                2299     459      35
 
EaeI y'GGCC_r

Cuts at:      0      97    1031    1298    1307    2646    2757    2793 
   Size:         97     934     267       9    1339     111      36

  Fragments arranged by size: 

                1339     934     267     111      97      36       9
 
EagI C'GGCC_G

Cuts at:      0      97    2793 
   Size:         97    2696
 
EarI CTCTTCn'nnn_

Cuts at:      0     131     178     342     808    2740    2793 
   Size:        131      47     164     466    1932      53

  Fragments arranged by size: 

                1932     466     164     131      53      47
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1561    2793 
   Size:       1561    1232
 
EcoICRI GAG'CTC

Cuts at:      0    2388    2793 
   Size:       2388     405
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     196     202     304     442     706     793     936 
   Size:        145      51       6     102     138     264      87     143

Cuts at:    936    1084    1096    1265    1684    1728    2069    2137    2182 
   Size:        148      12     169     419      44     341      68      45

Cuts at:   2182    2396    2461    2473    2692    2793 
   Size:        214      65      12     219     101

  Fragments arranged by size: 

                 419     341     264     219     214     169     148     145
                 143     138     102     101      87      68      65      51
                  45      44      12      12       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1160    1201    1492    1948    2411    2793 
   Size:       1160      41     291     456     463     382

  Fragments arranged by size: 

                1160     463     456     382     291      41
 
EcoO109I rG'GnC_Cy

Cuts at:      0     894     911     971    1176    1503    1504    1517    1774 
   Size:        894      17      60     205     327       1      13     257

Cuts at:   1774    1849    1850    1927    1935    1936    1991    2144    2351 
   Size:         75       1      77       8       1      55     153     207

Cuts at:   2351    2793 
   Size:        442

  Fragments arranged by size: 

                 894     442     327     257     207     205     153      77
                  75      60      55      17      13       8       1       1
                   1
 
HaeII r_GCGC'y

Cuts at:      0     965    1128    1380    1609    2092    2793 
   Size:        965     163     252     229     483     701

  Fragments arranged by size: 

                 965     701     483     252     229     163
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     352     384    1390    1422    1431    1463    2290    2314 
   Size:        352      32    1006      32       9      32     827      24

Cuts at:   2314    2322    2346    2665    2697    2793 
   Size:          8      24     319      32      96

  Fragments arranged by size: 

                1006     827     352     319      96      32      32      32
                  32      24      24       9       8
 
KasI G'GCGC_C

Cuts at:      0     961    2793 
   Size:        961    1832
 
MscI TGG'CCA

Cuts at:      0    1300    1309    2648    2759    2793 
   Size:       1300       9    1339     111      34

  Fragments arranged by size: 

                1339    1300     111      34       9
 
MslI CAynn'nnrTG

Cuts at:      0     298    1051    1720    2793 
   Size:        298     753     669    1073

  Fragments arranged by size: 

                1073     753     669     298
 
MspA1I CmG'CkG

Cuts at:      0     174     813     908    1234    1285    1458    1638    1662 
   Size:        174     639      95     326      51     173     180      24

Cuts at:   1662    1748    1755    2772    2793 
   Size:         86       7    1017      21

  Fragments arranged by size: 

                1017     639     326     180     174     173      95      86
                  51      24      21       7
 
NaeI GCC'GGC

Cuts at:      0    1031    2793 
   Size:       1031    1762
 
NarI GG'CG_CC

Cuts at:      0     962    2793 
   Size:        962    1831
 
NcoI C'CATG_G

Cuts at:      0     239     761    2579    2624    2793 
   Size:        239     522    1818      45     169

  Fragments arranged by size: 

                1818     522     239     169      45
 
NgoMIV G'CCGG_C

Cuts at:      0    1029    2793 
   Size:       1029    1764
 
NspI r_CATG'y

Cuts at:      0    1620    2793 
   Size:       1620    1173
 
PasI CC'CwG_GG

Cuts at:      0     399     897     967    1534    1770    2793 
   Size:        399     498      70     567     236    1023

  Fragments arranged by size: 

                1023     567     498     399     236      70
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2241    2793 
   Size:       2241     552
 
PfoI T'CCnGG_A

Cuts at:      0    2450    2558    2793 
   Size:       2450     108     235

  Fragments arranged by size: 

                2450     235     108
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     431     463     985    1017    1726    1758    2793 
   Size:        431      32     522      32     709      32    1035

  Fragments arranged by size: 

                1035     709     522     431      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     911    1517    1991    2144    2351    2793 
   Size:        911     606     474     153     207     442

  Fragments arranged by size: 

                 911     606     474     442     207     153
 
PspOMI G'GGCC_C

Cuts at:      0     970    1503    1773    1849    1935    2793 
   Size:        970     533     270      76      86     858

  Fragments arranged by size: 

                 970     858     533     270      86      76
 
PstI C_TGCA'G

Cuts at:      0     791     818    1469    1484    1583    1661    2132    2793 
   Size:        791      27     651      15      99      78     471     661

  Fragments arranged by size: 

                 791     661     651     471      99      78      27      15
            
 
PvuII CAG'CTG

Cuts at:      0     174     813    1285    1458    1638    1662    1748    1755 
   Size:        174     639     472     173     180      24      86       7

Cuts at:   1755    2772    2793 
   Size:       1017      21

  Fragments arranged by size: 

                1017     639     472     180     174     173      86      24
                  21       7
 
SacI G_AGCT'C

Cuts at:      0    2390    2793 
   Size:       2390     403
 
SanDI GG'GwC_CC

Cuts at:      0     911    1991    2144    2351    2793 
   Size:        911    1080     153     207     442

  Fragments arranged by size: 

                1080     911     442     207     153
 
SapI GCTCTTCn'nnn_

Cuts at:      0     342     808    2793 
   Size:        342     466    1985

  Fragments arranged by size: 

                1985     466     342
 
SbfI CC_TGCA'GG

Cuts at:      0    1469    2793 
   Size:       1469    1324
 
SfcI C'TryA_G

Cuts at:      0     787     814    1465    1480    1579    1657    2128    2793 
   Size:        787      27     651      15      99      78     471     665

  Fragments arranged by size: 

                 787     665     651     471      99      78      27      15
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1306    2793 
   Size:       1306    1487
 
SfoI GGC'GCC

Cuts at:      0     963    2793 
   Size:        963    1830
 
SmaI CCC'GGG

Cuts at:      0    1416    1510    1847    2793 
   Size:       1416      94     337     946

  Fragments arranged by size: 

                1416     946     337      94
 
SmlI C'TyrA_G

Cuts at:      0     706    1651    2229    2793 
   Size:        706     945     578     564

  Fragments arranged by size: 

                 945     706     578     564
 
SphI G_CATG'C

Cuts at:      0    1620    2793 
   Size:       1620    1173
 
StuI AGG'CCT

Cuts at:      0     231    1274    1471    2793 
   Size:        231    1043     197    1322

  Fragments arranged by size: 

                1322    1043     231     197
 
StyI C'CwwG_G

Cuts at:      0     239     677     761    1172    2579    2624    2793 
   Size:        239     438      84     411    1407      45     169

  Fragments arranged by size: 

                1407     438     411     239     169      84      45
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     487    1799    2138    2592    2793 
   Size:        487    1312     339     454     201

  Fragments arranged by size: 

                1312     487     454     339     201
 
TatI w'GTAC_w

Cuts at:      0     719    2793 
   Size:        719    2074
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1469    1501    2411    2443    2767    2793 
   Size:       1469      32     910      32     324      26

  Fragments arranged by size: 

                1469     910     324      32      32      26
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     246    1317    2622    2793 
   Size:        246    1071    1305     171

  Fragments arranged by size: 

                1305    1071     246     171
 
XmaI C'CCGG_G

Cuts at:      0    1414    1508    1845    2793 
   Size:       1414      94     337     948

  Fragments arranged by size: 

                1414     948     337      94
 
XmnI GAAnn'nnTTC

Cuts at:      0    1738    2793 
   Size:       1738    1055
 

 Enzymes that do cut:

     AcuI     AfeI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI
     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BsrBI    BsrFI    BssSI   BstAPI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI    CspCI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     KasI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI    PflMI     PfoI     PpiI    PpuMI   PspOMI     PstI    PvuII
     SacI    SanDI     SapI     SbfI     SfcI     SfiI     SfoI     SmaI
     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BciVI     BclI    BfrBI    BglII     BmtI     BplI    BsaAI    BsaBI
    BsaWI    BsiWI    BsmBI    BspEI    BspMI    BsrDI    BsrGI   BssHII
    BstBI   BstEII  BstZ17I     ClaI     DraI   DraIII     DrdI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI     ScaI    SexAI     SgfI
    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI  Tth111I     XbaI
     XhoI     ZraI
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