Restriction map for pF1KSDA1437
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

Acc65I      AclI        AflIII      AgeI        AocI        
AscI        AseI        AsnI        Asp718I     AviII       
AvrII       BbeI        BbrPI       BciVI       BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaI        
BsiWI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrGI       BssHII      BstBI       BstXI       
Bsu36I      CelII       ClaI        CpoI        DraI        
DrdI        EagI        EclXI       Eco52I      Eco81I      
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MunI        NarI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
SspBI       SspI        StuI        SwaI        XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII        1099  GACGTC               GACGT^C
AcsI          433  AAATTC               [GA]^AATT[CT]
AflII        1930  CTTAAG               C^TTAAG
AhdI          583  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      1163  AGCGCT               AGC^GCT
ApoI          433  AAATTC               [GA]^AATT[CT]
AspEI         583  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          642  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         2386  CCCGAG               C^[CT]CG[GA]G
BalI          941  TGGCCA               TGG^CCA
BamHI          53  GGATCC               G^GATCC
BbvCI        2073  CCTCAGC              CCTCAGC(-5/-2)
BcgI         2012  GCAACAAGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          815  TGATCA               T^GATCA
BfrI         1930  CTTAAG               C^TTAAG
BsiEI         596  CGACCG               CG[GA][CT]^CG
BsoBI        2386  CCCGAG               C^[CT]CG[GA]G
BssSI         507  CACGAG               CACGAG(-5/-1)
Bst1107I     1011  GTATAC               GTA^TAC
BstAPI       2229  GCAGTCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I      1011  GTATAC               GTA^TAC
DraIII        463  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      583  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1163  AGCGCT               AGC^GCT
EcoNI        1179  CCTGTCGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          815  TGATCA               T^GATCA
MluNI         941  TGGCCA               TGG^CCA
MscI          941  TGGCCA               TGG^CCA
MseI         1931  TTAA                 T^TAA
NaeI          552  GCCGGC               GCC^GGC
NcoI          578  CCATGG               C^CATGG
NgoMIV        552  GCCGGC               G^CCGGC
PflFI         642  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         933  CCACCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII        1210  CAGCTG               CAG^CTG
SbfI         1845  CCTGCAGG             CCTGCA^GG
Sse8387I     1845  CCTGCAGG             CCTGCA^GG
Tru9I        1931  TTAA                 T^TAA
Tsp509I       434  AATT                 ^AATT
Tth111I       642  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        933  CCACCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          446  CCAGCTCGAAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI          847  GTCTAC               GT^[AC][GT]AC
             1011  GTATAC              
AccIII        749  TCCGGA               T^CCGGA
             2172  TCCGGA              
AcyI          963  GGCGTC               G[GA]^CG[CT]C
             1099  GACGTC              
Alw44I        856  GTGCAC               G^TGCAC
             1699  GTGCAC              
ApaLI         856  GTGCAC               G^TGCAC
             1699  GTGCAC              
BanI          308  GGCACC               G^G[CT][GA]CC
             1732  GGCACC              
BcnI          207  CCGGG                CC^[GC]GG
              228  CCCGG               
BfaI          982  CTAG                 C^TAG
             2137  CTAG                
BsaAI         322  TACGTG               [CT]AC^GT[GA]
              853  TACGTG              
BsaHI         963  GGCGTC               G[GA]^CG[CT]C
             1099  GACGTC              
BseAI         749  TCCGGA               T^CCGGA
             2172  TCCGGA              
BsmBI         682  GAGACG               CGTCTC(1/5)
             2161  GAGACG              
BsmFI         127  GGGAC                GGGAC(10/14)
              770  GGGAC               
BspEI         749  TCCGGA               T^CCGGA
             2172  TCCGGA              
BsrDI        1392  CATTGC               GCAATG(2/0)
              194  GCAATG              
BstEII        138  GGTCACC              G^GT[ACGT]ACC
              177  GGTCACC             
BstPI         138  GGTCACC              G^GT[ACGT]ACC
              177  GGTCACC             
EcoO65I       138  GGTCACC              G^GT[ACGT]ACC
              177  GGTCACC             
Hin1I         963  GGCGTC               G[GA]^CG[CT]C
             1099  GACGTC              
MaeI          982  CTAG                 C^TAG
             2137  CTAG                
MroI          749  TCCGGA               T^CCGGA
             2172  TCCGGA              
NciI          207  CCGGG                CC^[GC]GG
              228  CCCGG               
NspI          791  ACATGC               [GA]CATG^[CT]
             1007  GCATGT              
PpuMI        1346  AGGTCCT              [GA]G^G[AT]CC[CT]
             2363  AGGACCT             
SapI         2040  GCTCTTC              GCTCTTC(1/4)
             2178  GCTCTTC             
ScaI          368  AGTACT               AGT^ACT
             1049  AGTACT              
SexAI         380  ACCTGGT              A^CC[AT]GGT
             1331  ACCTGGT             
TfiI            3  GATTC                G^A[AT]TC
              199  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       ApaI        Asp700I     AspHI       AvaII       
BanII       BbsI        BbvI        BglI        BmyI        
BpmI        BpuAI       BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
Bsp1286I    BspMI       BsrBI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NdeII       
NlaIII      NlaIV       PleI        PstI        RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     XhoII       XmnI        
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