Restriction map for pF1KSDA1433
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflIII      AgeI        AhdI        
Alw44I      AocI        ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BglII       BlnI        BsaAI       BsaBI       BsaHI       
BsaI        BseAI       BsiEI       BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstYI       
BstZ17I     Bsu36I      ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeII       MamI        MflI        
MluI        MroI        MvnI        NaeI        NarI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AflII         247  CTTAAG               C^TTAAG
Aor51HI       512  AGCGCT               AGC^GCT
BalI         1047  TGGCCA               TGG^CCA
BanI          320  GGCACC               G^G[CT][GA]CC
BbsI         1837  GAAGAC               GAAGAC(2/6)
BciVI         372  GGATAC               GTATCC(6/5)
BcnI          636  CCGGG                CC^[GC]GG
BfrI          247  CTTAAG               C^TTAAG
BglI           91  GCCTTAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          606  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      606  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1837  GAAGAC               GAAGAC(2/6)
BsaWI         773  ACCGGA               [AT]^CCGG[AT]
BsgI          225  CTGCAC               GTGCAG(16/14)
BsrFI        1290  GCCGGT               [GA]^CCGG[CT]
BstXI        1490  CCAAGCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         606  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1290  GCCGGT               [GA]^CCGG[CT]
DraI          807  TTTAAA               TTT^AAA
EaeI         1047  TGGCCA               [CT]^GGCC[GA]
Eco47III      512  AGCGCT               AGC^GCT
EcoNI        1648  CCTACTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       966  CCATGG               C^C[AT][AT]GG
FauI          518  CCCGC                CCCGC(4/6)
HaeII         512  AGCGCT               [GA]GCGC^[CT]
MfeI          865  CAATTG               C^AATTG
MluNI        1047  TGGCCA               TGG^CCA
MscI         1047  TGGCCA               TGG^CCA
MunI          865  CAATTG               C^AATTG
NciI          636  CCGGG                CC^[GC]GG
NcoI          966  CCATGG               C^CATGG
NdeI         1031  CATATG               CA^TATG
NheI         1324  GCTAGC               G^CTAGC
PshAI        1191  GACTCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         1230  GAGCTC               GAGCT^C
StyI          966  CCATGG               C^C[AT][AT]GG
XcmI         1419  CCAACAAGCCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcsI          442  GAATTT               [GA]^AATT[CT]
             1393  AAATTT              
AfaI         1294  GTAC                 GT^AC
             1350  GTAC                
AlwNI         899  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1848  CAGCAGCTG           
ApoI          442  GAATTT               [GA]^AATT[CT]
             1393  AAATTT              
AspHI        1230  GAGCTC               G[AT]GC[AT]^C
             1522  GTGCTC              
BsiHKAI      1230  GAGCTC               G[AT]GC[AT]^C
             1522  GTGCTC              
BsiYI         612  CCTGAAATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1648  CCTACTCCAGG         
BslI          612  CCTGAAATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1648  CCTACTCCAGG         
CfoI          285  GCGC                 GCG^C
              513  GCGC                
DraII          74  GGGACCT              [GA]G^G[ACGT]CC[CT]
              824  AGGACCT             
DsaI          966  CCATGG               C^C[GA][CT]GG
             1120  CCACGG              
EcoO109I       74  GGGACCT              [GA]G^G[ACGT]CC[CT]
              824  AGGACCT             
HhaI          285  GCGC                 GCG^C
              513  GCGC                
HinP1I        285  GCGC                 G^CGC
              513  GCGC                
PleI          429  GACTC                GAGTC(4/5)
             1191  GACTC               
PpuMI          74  GGGACCT              [GA]G^G[AT]CC[CT]
              824  AGGACCT             
PstI         1088  CTGCAG               CTGCA^G
             1832  CTGCAG              
RsaI         1294  GTAC                 GT^AC
             1350  GTAC                
SphI          287  GCATGC               GCATG^C
              363  GCATGC              
TaqI          731  TCGA                 T^CGA
             1599  TCGA                
TthHB8I       731  TCGA                 T^CGA
             1599  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BanII       
BbvI        BfaI        BmyI        BpmI        BsaJI       
BseRI       BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       EarI        EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PvuII       SapI        
Sau3AI      Sau96I      ScrFI       SfcI        SmlI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       
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