(Circular) (Six-base) MAPSORT of: pF1KSDA1433.seq Check: 2959 from: 1 to: 5054 pF1KSDA1433 5054 bp With 182 enzymes: SgfI * June 20, 2008 10:35 .. AccI GT'mk_AC Cuts at: 2092 2092 Size: 5054 Acc65I G'GTAC_C Cuts at: 2070 2070 Size: 5054 AclI AA'CG_TT Cuts at: 4894 4894 Size: 5054 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 337 527 2879 3311 4058 4326 337 Size: 190 2352 432 747 268 1065 Fragments arranged by size: 2352 1065 747 432 268 190 AfeI AGC'GCT Cuts at: 640 640 Size: 5054 AflII C'TTAA_G Cuts at: 373 373 Size: 5054 AflIII A'CryG_T Cuts at: 2406 3510 4487 2406 Size: 1104 977 2973 Fragments arranged by size: 2973 1104 977 AgeI A'CCGG_T Cuts at: 3442 3442 Size: 5054 AleI CACnn'nnGTG Cuts at: 1645 1645 Size: 5054 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 418 506 418 Size: 88 4966 AlwNI CAG_nnn'CTG Cuts at: 1030 1979 2359 3926 1030 Size: 949 380 1567 2158 Fragments arranged by size: 2158 1567 949 380 ApaLI G'TGCA_C Cuts at: 3824 3824 Size: 5054 ApoI r'AATT_y Cuts at: 568 1519 2058 4407 4663 568 Size: 951 539 2349 256 959 Fragments arranged by size: 2349 959 951 539 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5054 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5054 AvaI C'yCGr_G Cuts at: 2074 2074 Size: 5054 BamHI G'GATC_C Cuts at: 2079 2079 Size: 5054 BanI G'GyrC_C Cuts at: 446 2070 2484 2732 2767 446 Size: 1624 414 248 35 2733 Fragments arranged by size: 2733 1624 414 248 35 BanII G_rGCy'C Cuts at: 1360 1570 1807 2068 3098 1360 Size: 210 237 261 1030 3316 Fragments arranged by size: 3316 1030 261 237 210 BbeI G_GCGC'C Cuts at: 2488 2736 2488 Size: 248 4806 BbsI GAAGACnn'nnnn_ Cuts at: 1970 1970 Size: 5054 BciVI GTATCCnnnnn_n' Cuts at: 492 2952 3713 5054 492 Size: 2460 761 1341 492 Fragments arranged by size: 2460 1341 761 492 BclI T'GATC_A Cuts at: 2574 2574 Size: 5054 BglI GCCn_nnn'nGGC Cuts at: 223 223 Size: 5054 BglII A'GATC_T Cuts at: 2569 4606 2569 Size: 2037 3017 BlpI GC'TnA_GC Cuts at: 733 2166 733 Size: 1433 3621 Bme1580I G_kGCm'C Cuts at: 2679 2772 3828 2679 Size: 93 1056 3905 Fragments arranged by size: 3905 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1133 1328 2266 2347 2682 4826 1133 Size: 195 938 81 335 2144 1361 Fragments arranged by size: 2144 1361 938 335 195 81 BmtI G_CTAG'C Cuts at: 1454 1454 Size: 5054 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 572 657 3421 572 Size: 85 2764 2205 Fragments arranged by size: 2764 2205 85 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1304 1638 1763 1799 1304 Size: 334 125 36 4559 Fragments arranged by size: 4559 334 125 36 Bpu10I CC'TnA_GC Cuts at: 1801 1801 Size: 5054 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 490 697 2230 3109 3601 3899 490 Size: 207 1533 879 492 298 1645 Fragments arranged by size: 1645 1533 879 492 298 207 BsaI GGTCTCn'nnnn_ Cuts at: 4758 4758 Size: 5054 BsaAI yAC'GTr Cuts at: 2046 2407 2046 Size: 361 4693 BsaBI GATnn'nnATC Cuts at: 6 2568 4605 6 Size: 2562 2037 455 Fragments arranged by size: 2562 2037 455 BsaHI Gr'CG_yC Cuts at: 2485 2733 3435 4931 2485 Size: 248 702 1496 2608 Fragments arranged by size: 2608 1496 702 248 BsaWI w'CCGG_w Cuts at: 899 2243 2764 3442 3716 3863 4274 899 Size: 1344 521 678 274 147 411 1679 Fragments arranged by size: 1679 1344 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 503 533 1386 1416 1884 1914 503 Size: 30 853 30 468 30 3643 Fragments arranged by size: 3643 853 468 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 649 1374 1377 649 Size: 725 3 4326 Fragments arranged by size: 4326 725 3 BseYI C'CCAG_C Cuts at: 422 1369 2479 3237 3814 4310 422 Size: 947 1110 758 577 496 1166 Fragments arranged by size: 1166 1110 947 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 336 336 Size: 5054 BsiEI CG_ry'CG Cuts at: 104 2596 2642 3850 104 Size: 2492 46 1208 1308 Fragments arranged by size: 2492 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1360 1652 2068 2846 3036 3828 1360 Size: 292 416 778 190 792 2586 Fragments arranged by size: 2586 792 778 416 292 190 Bsp1286I G_dGCh'C Cuts at: 1360 1570 1652 1807 2068 2679 2772 2846 3036 Size: 210 82 155 261 611 93 74 190 Cuts at: 3036 3098 3828 1360 Size: 62 730 2586 Fragments arranged by size: 2586 730 611 261 210 190 155 93 82 74 62 BspEI T'CCGG_A Cuts at: 4274 4274 Size: 5054 BspHI T'CATG_A Cuts at: 4230 4230 Size: 5054 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2104 2318 2620 3001 2104 Size: 214 302 381 4157 Fragments arranged by size: 4157 381 302 214 BsrBI CCG'CTC Cuts at: 50 3346 3400 4884 5049 50 Size: 3296 54 1484 165 55 Fragments arranged by size: 3296 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2966 2966 Size: 5054 BsrFI r'CCGG_y Cuts at: 1416 3052 3233 3442 1416 Size: 1636 181 209 3028 Fragments arranged by size: 3028 1636 209 181 BssSI C'ACGA_G Cuts at: 3325 3683 3325 Size: 358 4696 BstAPI GCAn_nnn'nTGC Cuts at: 2431 2431 Size: 5054 BstBI TT'CG_AA Cuts at: 110 3415 110 Size: 3305 1749 BstXI CCAn_nnnn'nTGG Cuts at: 132 1623 132 Size: 1491 3563 BstYI r'GATC_y Cuts at: 1 2079 2546 2569 2904 3150 4162 4248 4260 Size: 2078 467 23 335 246 1012 86 12 Cuts at: 4260 4606 1 Size: 346 449 Fragments arranged by size: 2078 1012 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 1092 1246 1092 Size: 154 4900 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3004 3185 3348 3004 Size: 181 163 4710 Fragments arranged by size: 4710 181 163 BtsI GCAGTG_nn' Cuts at: 397 1376 1739 1955 2297 397 Size: 979 363 216 342 3154 Fragments arranged by size: 3154 979 363 342 216 DraI TTT'AAA Cuts at: 935 2053 935 Size: 1118 3936 DrdI GACnn_nn'nnGTC Cuts at: 2760 3618 2760 Size: 858 4196 EaeI y'GGCC_r Cuts at: 1173 2639 2813 3204 3231 4576 1173 Size: 1466 174 391 27 1345 1651 Fragments arranged by size: 1651 1466 1345 391 174 27 EagI C'GGCC_G Cuts at: 2639 2639 Size: 5054 EarI CTCTTCn'nnn_ Cuts at: 626 751 776 887 1350 1416 1989 3077 3287 Size: 125 25 111 463 66 573 1088 210 Cuts at: 3287 626 Size: 2393 Fragments arranged by size: 2393 1088 573 463 210 125 111 66 25 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3262 3572 3718 4864 3262 Size: 310 146 1146 3452 Fragments arranged by size: 3452 1146 310 146 EcoICRI GAG'CTC Cuts at: 1358 2066 1358 Size: 708 4346 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 337 527 1304 1638 1763 1799 2879 3311 4058 Size: 190 777 334 125 36 1080 432 747 Cuts at: 4058 4326 337 Size: 268 1065 Fragments arranged by size: 1080 1065 777 747 432 334 268 190 125 36 EcoNI CCTnn'n_nnAGG Cuts at: 1778 1778 Size: 5054 EcoO109I rG'GnC_Cy Cuts at: 201 951 2193 201 Size: 750 1242 3062 Fragments arranged by size: 3062 1242 750 EcoRI G'AATT_C Cuts at: 2058 2058 Size: 5054 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 205 825 3073 205 Size: 620 2248 2186 Fragments arranged by size: 2248 2186 620 FspI TGC'GCA Cuts at: 2835 2835 Size: 5054 HaeII r_GCGC'y Cuts at: 642 2488 2736 3758 4737 642 Size: 1846 248 1022 979 959 Fragments arranged by size: 1846 1022 979 959 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 503 535 1302 1334 1914 1946 2896 2926 2928 Size: 32 767 32 580 32 950 30 2 Cuts at: 2928 2958 503 Size: 30 2599 Fragments arranged by size: 2599 950 767 580 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 2093 2093 Size: 5054 KasI G'GCGC_C Cuts at: 2484 2732 2484 Size: 248 4806 KpnI G_GTAC'C Cuts at: 2074 2074 Size: 5054 MfeI C'AATT_G Cuts at: 991 991 Size: 5054 MluI A'CGCG_T Cuts at: 4487 4487 Size: 5054 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 545 3725 3909 545 Size: 3180 184 1690 Fragments arranged by size: 3180 1690 184 MscI TGG'CCA Cuts at: 1175 2815 1175 Size: 1640 3414 MslI CAynn'nnrTG Cuts at: 526 670 1045 1645 3043 3170 526 Size: 144 375 600 1398 127 2410 Fragments arranged by size: 2410 1398 600 375 144 127 MspA1I CmG'CkG Cuts at: 426 486 627 793 1623 1979 2165 2479 2839 Size: 60 141 166 830 356 186 314 360 Cuts at: 2839 3852 4097 426 Size: 1013 245 1383 Fragments arranged by size: 1383 1013 830 360 356 314 245 186 166 141 60 NaeI GCC'GGC Cuts at: 3235 3235 Size: 5054 NarI GG'CG_CC Cuts at: 2485 2733 2485 Size: 248 4806 NcoI C'CATG_G Cuts at: 1092 1092 Size: 5054 NdeI CA'TA_TG Cuts at: 1158 1158 Size: 5054 NgoMIV G'CCGG_C Cuts at: 3233 3233 Size: 5054 NheI G'CTAG_C Cuts at: 1450 1450 Size: 5054 NspI r_CATG'y Cuts at: 405 417 493 531 2107 2607 3514 405 Size: 12 76 38 1576 500 907 1945 Fragments arranged by size: 1945 1576 907 500 76 38 12 PciI A'CATG_T Cuts at: 3510 3510 Size: 5054 PfoI T'CCnGG_A Cuts at: 1815 1815 Size: 5054 PmeI GTTT'AAAC Cuts at: 2053 2053 Size: 5054 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4193 4225 4193 Size: 32 5022 PpuMI rG'GwC_Cy Cuts at: 201 951 201 Size: 750 4304 PshAI GACnn'nnGTC Cuts at: 1321 1321 Size: 5054 PstI C_TGCA'G Cuts at: 1218 1962 2101 2786 1218 Size: 744 139 685 3486 Fragments arranged by size: 3486 744 685 139 PvuI CG_AT'CG Cuts at: 104 104 Size: 5054 PvuII CAG'CTG Cuts at: 426 627 793 1623 1979 2479 2839 426 Size: 201 166 830 356 500 360 2641 Fragments arranged by size: 2641 830 500 360 356 201 166 RsrII CG'GwC_CG Cuts at: 3249 3249 Size: 5054 SacI G_AGCT'C Cuts at: 1360 2068 1360 Size: 708 4346 SalI G'TCGA_C Cuts at: 2091 2091 Size: 5054 SapI GCTCTTCn'nnn_ Cuts at: 751 1416 1989 3077 3287 751 Size: 665 573 1088 210 2518 Fragments arranged by size: 2518 1088 665 573 210 SbfI CC_TGCA'GG Cuts at: 2101 2101 Size: 5054 ScaI AGT'ACT Cuts at: 4571 4571 Size: 5054 SfcI C'TryA_G Cuts at: 33 295 1214 1561 1958 2097 2782 3775 3966 Size: 262 919 347 397 139 685 993 191 Cuts at: 3966 33 Size: 1121 Fragments arranged by size: 1121 993 919 685 397 347 262 191 139 SfoI GGC'GCC Cuts at: 2486 2734 2486 Size: 248 4806 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5054 SmaI CCC'GGG Cuts at: 2076 2076 Size: 5054 SmlI C'TyrA_G Cuts at: 373 469 676 2209 3124 3616 3878 373 Size: 96 207 1533 915 492 262 1549 Fragments arranged by size: 1549 1533 915 492 262 207 96 SnaBI TAC'GTA Cuts at: 2046 2046 Size: 5054 SpeI A'CTAG_T Cuts at: 67 67 Size: 5054 SphI G_CATG'C Cuts at: 417 493 2107 2607 417 Size: 76 1614 500 2864 Fragments arranged by size: 2864 1614 500 76 StyI C'CwwG_G Cuts at: 1092 2188 2542 1092 Size: 1096 354 3604 Fragments arranged by size: 3604 1096 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1286 1881 1890 2637 2770 3229 3438 1286 Size: 595 9 747 133 459 209 2902 Fragments arranged by size: 2902 747 595 459 209 133 9 TatI w'GTAC_w Cuts at: 4569 4569 Size: 5054 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1731 1763 1884 1916 4571 4603 1731 Size: 32 121 32 2655 32 2182 Fragments arranged by size: 2655 2182 121 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2851 2851 Size: 5054 XbaI T'CTAG_A Cuts at: 2085 2085 Size: 5054 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1552 2549 1552 Size: 997 4057 XmaI C'CCGG_G Cuts at: 2074 2074 Size: 5054 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI PciI PfoI PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AhdI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI NotI NruI NsiI PacI PasI PflMI PmlI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI XmnI ZraI