Restriction map for pF1KA1429
##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AgeI        AhdI        Alw44I      ApaI        
ApaLI       AscI        Asp718I     AspEI       AviII       
AvrII       BanI        BbeI        BbrPI       BbvCI       
BcnI        BlnI        BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        BspEI       
BsrBI       BsrFI       BssHII      BstBI       BstUI       
Cfr10I      CpoI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco52I      FauI        
FseI        FspI        HapII       HgaI        Hin1I       
HpaII       KasI        KpnI        KspI        MluI        
MroI        MspI        MvnI        NaeI        NarI        
NciI        NcoI        NgoMIV      NotI        NruI        
NspV        PacI        PinAI       PmaCI       PmeI        
PmlI        Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
StuI        SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AocI          823  CCTGAGG              CC^T[ACGT]AGG
AspI         1522  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        3070  GGATCC               G^GATCC
BcgI         1764  CGACACAGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         951  GTATCC               GTATCC(6/5)
BglI         2955  GCCTTGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI           58  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       58  GCTGAGC              GC^T[ACGT]AGC
Bsp1407I     2341  TGTACA               T^GTACA
BspDI        2755  ATCGAT               AT^CGAT
BspLU11I     4036  ACATGT               A^CATGT
BsrGI        2341  TGTACA               T^GTACA
BssSI        5367  CACGAG               CACGAG(-5/-1)
BstAPI       3500  GCACAACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        2241  CCAATCTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        823  CCTGAGG              CC^T[ACGT]AGG
CelII          58  GCTGAGC              GC^T[ACGT]AGC
ClaI         2755  ATCGAT               AT^CGAT
Eco81I        823  CCTGAGG              CC^T[ACGT]AGG
EcoNI        2836  CCTGTTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         675  GAATTC               G^AATTC
MspA1I       2429  CAGCTG               C[AC]G^C[GT]G
NheI         3999  GCTAGC               G^CTAGC
PaeR7I       5086  CTCGAG               C^TCGAG
PflFI        1522  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         604  CCAGTGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         558  AGGACCC              [GA]G^G[AT]CC[CT]
PshAI        4035  GACATGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         4304  CTGCAG               CTGCA^G
PvuII        2429  CAGCTG               CAG^CTG
SexAI        3667  ACCAGGT              A^CC[AT]GGT
SspBI        2341  TGTACA               T^GTACA
SspI          698  AATATT               AAT^ATT
Tth111I      1522  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        604  CCAGTGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         5202  TCTAGA               T^CTAGA
XcmI         2498  CCAAAGAAGCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         5086  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AflII        1495  CTTAAG               C^TTAAG
             4438  CTTAAG              
AseI         3736  ATTAAT               AT^TAAT
             4297  ATTAAT              
AsnI         3736  ATTAAT               AT^TAAT
             4297  ATTAAT              
BfrI         1495  CTTAAG               C^TTAAG
             4438  CTTAAG              
BsaAI        1480  CACGTA               [CT]AC^GT[GA]
             2801  TACGTG              
BspHI         399  TCATGA               T^CATGA
             4905  TCATGA              
BspMI        3505  ACCTGC               ACCTGC(4/8)
             4830  ACCTGC              
Bst1107I     3651  GTATAC               GTA^TAC
             4895  GTATAC              
BstZ17I      3651  GTATAC               GTA^TAC
             4895  GTATAC              
DraII         558  AGGACCC              [GA]G^G[ACGT]CC[CT]
             5135  AGGCCCT             
EcoO109I      558  AGGACCC              [GA]G^G[ACGT]CC[CT]
             5135  AGGCCCT             
EcoRV        2021  GATATC               GAT^ATC
             3187  GATATC              
HpaI         1152  GTTAAC               GTT^AAC
             3048  GTTAAC              
PshBI        3736  ATTAAT               AT^TAAT
             4297  ATTAAT              
RcaI          399  TCATGA               T^CATGA
             4905  TCATGA              
SapI         3679  GCTCTTC              GCTCTTC(1/4)
             3902  GCTCTTC             
ScaI         2489  AGTACT               AGT^ACT
             4500  AGTACT              


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       Aor51HI     ApoI        
Asp700I     AspHI       AvaI        AvaII       BalI        
BanII       BbsI        BbvI        BclI        BfaI        
BglII       BmyI        BpmI        BpuAI       BsaBI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrDI       BsrI        BssKI       BstEII      
BstF5I      BstNI       BstPI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
EaeI        EarI        Eco47III    EcoO65I     EcoRII      
EcoT14I     EcoT22I     FbaI        Fnu4HI      FokI        
HaeII       HaeIII      HhaI        HinP1I      HincII      
HindII      HindIII     HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MamI        
MboI        MboII       MfeI        MflI        MluNI       
MnlI        MscI        MseI        MslI        MunI        
MvaI        MwoI        NdeI        NdeII       NlaIII      
NlaIV       NsiI        NspI        PleI        RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SphI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmnI        
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