Restriction map for pF1KA1429
 (Linear) (Six-base) MAPSORT of: KIAA1429.seq  Check: 3436  from: 1  to: 5391



KIAA1429.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:27  ..

AccI GT'mk_AC

Cuts at:      0     812    1295    3652    4896    4941    5391 
   Size:        812     483    2357    1244      45     450

  Fragments arranged by size: 

                2357    1244     812     483     450      45
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     977    1001    1035    2918    3086    3338    3614    3929 
   Size:        977      24      34    1883     168     252     276     315

Cuts at:   3929    4507    4634    5391 
   Size:        578     127     757

  Fragments arranged by size: 

                1883     977     757     578     315     276     252     168
                 127      34      24
 
AfeI AGC'GCT

Cuts at:      0      57    1331    3107    4711    5391 
   Size:         57    1274    1776    1604     680

  Fragments arranged by size: 

                1776    1604    1274     680      57
 
AflII C'TTAA_G

Cuts at:      0    1495    4438    5391 
   Size:       1495    2943     953

  Fragments arranged by size: 

                2943    1495     953
 
AflIII A'CryG_T

Cuts at:      0    2802    2909    4036    5391 
   Size:       2802     107    1127    1355

  Fragments arranged by size: 

                2802    1355    1127     107
 
AleI CACnn'nnGTG

Cuts at:      0    2093    5391 
   Size:       2093    3298
 
AlwNI CAG_nnn'CTG

Cuts at:      0     610    1774    4449    4530    5391 
   Size:        610    1164    2675      81     861

  Fragments arranged by size: 

                2675    1164     861     610      81
 
ApoI r'AATT_y

Cuts at:      0     675     899    1122    1217    1317    2184    3176    4195 
   Size:        675     224     223      95     100     867     992    1019

Cuts at:   4195    4208    4350    5391 
   Size:         13     142    1041

  Fragments arranged by size: 

                1041    1019     992     867     675     224     223     142
                 100      95      13
 
AseI AT'TA_AT

Cuts at:      0    3737    4298    5391 
   Size:       3737     561    1093

  Fragments arranged by size: 

                3737    1093     561
 
AvaI C'yCGr_G

Cuts at:      0      51    5086    5188    5391 
   Size:         51    5035     102     203

  Fragments arranged by size: 

                5035     203     102      51
 
BamHI G'GATC_C

Cuts at:      0    3070    5391 
   Size:       3070    2321
 
BanII G_rGCy'C

Cuts at:      0     147     659    1413    2202    4275    4845    5391 
   Size:        147     512     754     789    2073     570     546

  Fragments arranged by size: 

                2073     789     754     570     546     512     147
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     989    1732    2143    2573    3620    5391 
   Size:        989     743     411     430    1047    1771

  Fragments arranged by size: 

                1771    1047     989     743     430     411
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1753    1787    5391 
   Size:       1753      34    3604

  Fragments arranged by size: 

                3604    1753      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     962    5391 
   Size:        962    4429
 
BclI T'GATC_A

Cuts at:      0    1445    1476    1758    2217    3873    4575    5391 
   Size:       1445      31     282     459    1656     702     816

  Fragments arranged by size: 

                1656    1445     816     702     459     282      31
 
BfrBI ATG'CAT

Cuts at:      0    2266    2555    4956    5391 
   Size:       2266     289    2401     435

  Fragments arranged by size: 

                2401    2266     435     289
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2961    5391 
   Size:       2961    2430
 
BglII A'GATC_T

Cuts at:      0    2370    2856    4629    5391 
   Size:       2370     486    1773     762

  Fragments arranged by size: 

                2370    1773     762     486
 
BlpI GC'TnA_GC

Cuts at:      0      59    5391 
   Size:         59    5332
 
Bme1580I G_kGCm'C

Cuts at:      0     243    5391 
   Size:        243    5148
 
BmtI G_CTAG'C

Cuts at:      0    4003    5391 
   Size:       4003    1388
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3954    5391 
   Size:       3954    1437
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     539    1970    2473    3830    3949    4471    4496    4721 
   Size:        539    1431     503    1357     119     522      25     225

Cuts at:   4721    5045    5391 
   Size:        324     346

  Fragments arranged by size: 

                1431    1357     539     522     503     346     324     225
                 119      25
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1397    1801    4985    5246    5391 
   Size:       1397     404    3184     261     145

  Fragments arranged by size: 

                3184    1397     404     261     145
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2242    3809    4258    4783    5391 
   Size:       2242    1567     449     525     608

  Fragments arranged by size: 

                2242    1567     608     525     449
 
BsaAI yAC'GTr

Cuts at:      0    1482    2803    5391 
   Size:       1482    1321    2588

  Fragments arranged by size: 

                2588    1482    1321
 
BsaBI GATnn'nnATC

Cuts at:      0    1832    4403    4628    5327    5391 
   Size:       1832    2571     225     699      64

  Fragments arranged by size: 

                2571    1832     699     225      64
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1968    1998    3110    3140    3326    3356    3803    3833 
   Size:       1968      30    1112      30     186      30     447      30

Cuts at:   3833    5391 
   Size:       1558

  Fragments arranged by size: 

                1968    1558    1112     447     186      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     542     623     841    2242    5074    5391 
   Size:        542      81     218    1401    2832     317

  Fragments arranged by size: 

                2832    1401     542     317     218      81
 
BseYI C'CCAG_C

Cuts at:      0     446    1372    5391 
   Size:        446     926    4019

  Fragments arranged by size: 

                4019     926     446
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2371    3006    3557    5391 
   Size:       2371     635     551    1834

  Fragments arranged by size: 

                2371    1834     635     551
 
BsiHKAI G_wGCw'C

Cuts at:      0    2088    2202    3136    4122    4275    4845    5075    5391 
   Size:       2088     114     934     986     153     570     230     316

  Fragments arranged by size: 

                2088     986     934     570     316     230     153     114
            
 
Bsp1286I G_dGCh'C

Cuts at:      0     147     243     659    1413    2088    2202    3136    4122 
   Size:        147      96     416     754     675     114     934     986

Cuts at:   4122    4275    4845    5075    5391 
   Size:        153     570     230     316

  Fragments arranged by size: 

                 986     934     754     675     570     416     316     230
                 153     147     114      96
 
BspHI T'CATG_A

Cuts at:      0     399    4905    5391 
   Size:        399    4506     486

  Fragments arranged by size: 

                4506     486     399
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    3514    4839    5391 
   Size:       3514    1325     552

  Fragments arranged by size: 

                3514    1325     552
 
BsrDI GCAATG_nn'

Cuts at:      0    2299    2311    3367    3881    5391 
   Size:       2299      12    1056     514    1510

  Fragments arranged by size: 

                2299    1510    1056     514      12
 
BsrGI T'GTAC_A

Cuts at:      0    2341    5391 
   Size:       2341    3050
 
BssSI C'ACGA_G

Cuts at:      0    5367    5391 
   Size:       5367      24
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3506    5391 
   Size:       3506    1885
 
BstEII G'GTnAC_C

Cuts at:      0    1708    2052    4462    5172    5391 
   Size:       1708     344    2410     710     219

  Fragments arranged by size: 

                2410    1708     710     344     219
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2248    5391 
   Size:       2248    3143
 
BstYI r'GATC_y

Cuts at:      0    1833    2370    2856    3070    3783    4629    5391 
   Size:       1833     537     486     214     713     846     762

  Fragments arranged by size: 

                1833     846     762     713     537     486     214
 
BstZ17I GTA'TAC

Cuts at:      0    3653    4897    5391 
   Size:       3653    1244     494

  Fragments arranged by size: 

                3653    1244     494
 
Bsu36I CC'TnA_GG

Cuts at:      0     824    5391 
   Size:        824    4567
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      31    5391 
   Size:         31    5360
 
BtsI GCAGTG_nn'

Cuts at:      0     974    2792    5258    5294    5391 
   Size:        974    1818    2466      36      97

  Fragments arranged by size: 

                2466    1818     974      97      36
 
ClaI AT'CG_AT

Cuts at:      0    2756    5391 
   Size:       2756    2635
 
DraI TTT'AAA

Cuts at:      0      45    1316    1512    4091    5391 
   Size:         45    1271     196    2579    1300

  Fragments arranged by size: 

                2579    1300    1271     196      45
 
EaeI y'GGCC_r

Cuts at:      0    1284    2436    3257    5026    5391 
   Size:       1284    1152     821    1769     365

  Fragments arranged by size: 

                1769    1284    1152     821     365
 
EarI CTCTTCn'nnn_

Cuts at:      0     617     716     773     827     839     854    1742    3038 
   Size:        617      99      57      54      12      15     888    1296

Cuts at:   3038    3242    3368    3686    3909    4526    4610    5146    5391 
   Size:        204     126     318     223     617      84     536     245

  Fragments arranged by size: 

                1296     888     617     617     536     318     245     223
                 204     126      99      84      57      54      15      12
            
 
EcoICRI GAG'CTC

Cuts at:      0    2200    4273    4843    5391 
   Size:       2200    2073     570     548

  Fragments arranged by size: 

                2200    2073     570     548
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     539     977    1001    1035    1970    2473    2918    3086 
   Size:        539     438      24      34     935     503     445     168

Cuts at:   3086    3338    3614    3830    3929    3949    4471    4496    4507 
   Size:        252     276     216      99      20     522      25      11

Cuts at:   4507    4634    4721    5045    5391 
   Size:        127      87     324     346

  Fragments arranged by size: 

                 935     539     522     503     445     438     346     324
                 276     252     216     168     127      99      87      34
                  25      24      20      11
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2840    5391 
   Size:       2840    2551
 
EcoO109I rG'GnC_Cy

Cuts at:      0     559    5136    5391 
   Size:        559    4577     255

  Fragments arranged by size: 

                4577     559     255
 
EcoRI G'AATT_C

Cuts at:      0     675    5391 
   Size:        675    4716
 
EcoRV GAT'ATC

Cuts at:      0    2023    3189    5391 
   Size:       2023    1166    2202

  Fragments arranged by size: 

                2202    2023    1166
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1297    1348    3310    4668    5391 
   Size:       1297      51    1962    1358     723

  Fragments arranged by size: 

                1962    1358    1297     723      51
 
HaeII r_GCGC'y

Cuts at:      0      59    1333    3109    4713    5391 
   Size:         59    1274    1776    1604     678

  Fragments arranged by size: 

                1776    1604    1274     678      59
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     394     426     999    1031    1468    1500    1719    1751 
   Size:        394      32     573      32     437      32     219      32

Cuts at:   1751    1983    2015    2184    2216    2506    2538    3326    3358 
   Size:        232      32     169      32     290      32     788      32

Cuts at:   3358    3547    3579    4165    4197    4681    4713    5391 
   Size:        189      32     586      32     484      32     678

  Fragments arranged by size: 

                 788     678     586     573     484     437     394     290
                 232     219     189     169      32      32      32      32
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1154    2848    2973    3050    4650    5391 
   Size:       1154    1694     125      77    1600     741

  Fragments arranged by size: 

                1694    1600    1154     741     125      77
 
HindIII A'AGCT_T

Cuts at:      0    1310    1514    1553    1597    1817    2629    3707    4372 
   Size:       1310     204      39      44     220     812    1078     665

Cuts at:   4372    5391 
   Size:       1019

  Fragments arranged by size: 

                1310    1078    1019     812     665     220     204      44
                  39
 
HpaI GTT'AAC

Cuts at:      0    1154    3050    5391 
   Size:       1154    1896    2341

  Fragments arranged by size: 

                2341    1896    1154
 
MfeI C'AATT_G

Cuts at:      0    1255    3547    4226    5391 
   Size:       1255    2292     679    1165

  Fragments arranged by size: 

                2292    1255    1165     679
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1962    2147    3578    4400    4413    5046    5391 
   Size:       1962     185    1431     822      13     633     345

  Fragments arranged by size: 

                1962    1431     822     633     345     185      13
 
MscI TGG'CCA

Cuts at:      0    1286    2438    3259    5391 
   Size:       1286    1152     821    2132

  Fragments arranged by size: 

                2132    1286    1152     821
 
MslI CAynn'nnrTG

Cuts at:      0      77     485    1534    1676    2093    2213    2304    3452 
   Size:         77     408    1049     142     417     120      91    1148

Cuts at:   3452    4073    4784    4961    5391 
   Size:        621     711     177     430

  Fragments arranged by size: 

                1148    1049     711     621     430     417     408     177
                 142     120      91      77
 
MspA1I CmG'CkG

Cuts at:      0    2431    5391 
   Size:       2431    2960
 
NdeI CA'TA_TG

Cuts at:      0     174    1011    1020    3122    3524    5391 
   Size:        174     837       9    2102     402    1867

  Fragments arranged by size: 

                2102    1867     837     402     174       9
 
NheI G'CTAG_C

Cuts at:      0    3999    5391 
   Size:       3999    1392
 
NsiI A_TGCA'T

Cuts at:      0    2268    2557    4958    5391 
   Size:       2268     289    2401     433

  Fragments arranged by size: 

                2401    2268     433     289
 
NspI r_CATG'y

Cuts at:      0     484    2555    2614    2825    3093    3447    4040    4960 
   Size:        484    2071      59     211     268     354     593     920

Cuts at:   4960    5391 
   Size:        431

  Fragments arranged by size: 

                2071     920     593     484     431     354     268     211
                  59
 
PciI A'CATG_T

Cuts at:      0    4036    5391 
   Size:       4036    1355
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     610    5391 
   Size:        610    4781
 
PfoI T'CCnGG_A

Cuts at:      0     555    3779    3807    4737    5391 
   Size:        555    3224      28     930     654

  Fragments arranged by size: 

                3224     930     654     555      28
 
PpuMI rG'GwC_Cy

Cuts at:      0     559    5391 
   Size:        559    4832
 
PshAI GACnn'nnGTC

Cuts at:      0    4039    5391 
   Size:       4039    1352
 
PsiI TTA'TAA

Cuts at:      0    2543    4798    5391 
   Size:       2543    2255     593

  Fragments arranged by size: 

                2543    2255     593
 
PspXI vC'TCGA_Gb

Cuts at:      0    5086    5391 
   Size:       5086     305
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    4737    4769    5391 
   Size:       4737      32     622

  Fragments arranged by size: 

                4737     622      32
 
PstI C_TGCA'G

Cuts at:      0    4308    5391 
   Size:       4308    1083
 
PvuII CAG'CTG

Cuts at:      0    2431    5391 
   Size:       2431    2960
 
SacI G_AGCT'C

Cuts at:      0    2202    4275    4845    5391 
   Size:       2202    2073     570     546

  Fragments arranged by size: 

                2202    2073     570     546
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3686    3909    5391 
   Size:       3686     223    1482

  Fragments arranged by size: 

                3686    1482     223
 
ScaI AGT'ACT

Cuts at:      0    2491    4502    5391 
   Size:       2491    2011     889

  Fragments arranged by size: 

                2491    2011     889
 
SexAI A'CCwGG_T

Cuts at:      0    3667    5391 
   Size:       3667    1724
 
SfcI C'TryA_G

Cuts at:      0    2281    4304    4677    5167    5391 
   Size:       2281    2023     373     490     224

  Fragments arranged by size: 

                2281    2023     490     373     224
 
SmlI C'TyrA_G

Cuts at:      0    1412    1495    1780    4438    5000    5086    5261    5391 
   Size:       1412      83     285    2658     562      86     175     130

  Fragments arranged by size: 

                2658    1412     562     285     175     130      86      83
            
 
SphI G_CATG'C

Cuts at:      0    2555    2825    3447    5391 
   Size:       2555     270     622    1944

  Fragments arranged by size: 

                2555    1944     622     270
 
SspI AAT'ATT

Cuts at:      0     700    5391 
   Size:        700    4691
 
StyI C'CwwG_G

Cuts at:      0     535    1281    2507    2956    5391 
   Size:        535     746    1226     449    2435

  Fragments arranged by size: 

                2435    1226     746     535     449
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2500    5391 
   Size:       2500    2891
 
TatI w'GTAC_w

Cuts at:      0    2341    2489    4500    5391 
   Size:       2341     148    2011     891

  Fragments arranged by size: 

                2341    2011     891     148
 
Tth111I GACn'n_nGTC

Cuts at:      0    1525    5391 
   Size:       1525    3866
 
XbaI T'CTAG_A

Cuts at:      0    5202    5391 
   Size:       5202     189
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2505    5391 
   Size:       2505    2886
 
XhoI C'TCGA_G

Cuts at:      0    5086    5391 
   Size:       5086     305
 
XmnI GAAnn'nnTTC

Cuts at:      0    1217    1901    3482    4520    4994    5391 
   Size:       1217     684    1581    1038     474     397

  Fragments arranged by size: 

                1581    1217    1038     684     474     397
 

 Enzymes that do cut:

     AccI     AcuI     AfeI    AflII   AflIII     AleI    AlwNI     ApoI
     AseI     AvaI    BamHI    BanII     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I     BmtI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI    BssSI   BstAPI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
     DraI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     HpaI     MfeI
     MmeI     MscI     MslI   MspA1I     NdeI     NheI     NsiI     NspI
     PciI    PflMI     PfoI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PstI    PvuII     SacI     SapI     ScaI    SexAI     SfcI     SmlI
     SphI     SspI     StyI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AgeI     AhdI     AlfI     AloI
     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI     BanI     BbeI
    BbvCI    BmgBI     BmrI   Bpu10I    BsaHI    BsaWI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BsrBI    BsrFI   BssHII    BstBI     BtgI
    CspCI   DraIII     DrdI     EagI     EciI     FseI     FspI    FspAI
     KasI     KpnI     MluI     NaeI     NarI     NcoI   NgoMIV     NotI
     NruI     PacI     PasI     PmeI     PmlI     PpiI   PspOMI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI     TstI
     XmaI     ZraI
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