Restriction map for pF1KSDA1427
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbrPI       BbvCI       BciVI       BfrI        
BglI        BglII       BlnI        BsaAI       BsaBI       
BsaWI       BseAI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstXI       BstYI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoRI       EcoRV       EcoT22I     FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        Ksp632I     MamI        MfeI        MflI        
MluI        MroI        MslI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TaqI        Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI          309  GTCTAC               GT^[AC][GT]AC
AcyI           31  GGCGCC               G[GA]^CG[CT]C
Asp700I       205  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI           31  GGCGCC               GGCGC^C
BbsI          831  GAAGAC               GAAGAC(2/6)
BcgI         1002  GCACCAGGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1076  TGATCA               T^GATCA
BfaI          805  CTAG                 C^TAG
BlpI         1042  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     1042  GCTAAGC              GC^T[ACGT]AGC
BpuAI         831  GAAGAC               GAAGAC(2/6)
BsaHI          31  GGCGCC               G[GA]^CG[CT]C
BsaI          436  GAGACC               GGTCTC(1/5)
BsiEI         738  CGACCG               CG[GA][CT]^CG
BstAPI       1263  GCACCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        1042  GCTAAGC              GC^T[ACGT]AGC
DraIII        754  CACAGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          574  GACTGCTACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI         1076  TGATCA               T^GATCA
HaeII          31  GGCGCC               [GA]GCGC^[CT]
HgaI          717  GACGC                GACGC(5/10)
Hin1I          31  GGCGCC               G[GA]^CG[CT]C
KasI           31  GGCGCC               G^GCGCC
KspI          121  CCGCGG               CCGC^GG
MaeI          805  CTAG                 C^TAG
MaeII         581  ACGT                 A^CGT
NaeI         1244  GCCGGC               GCC^GGC
NarI           31  GGCGCC               GG^CGCC
NgoMIV       1244  GCCGGC               G^CCGGC
NspI           95  ACATGC               [GA]CATG^[CT]
PleI          531  GACTC                GAGTC(4/5)
SacII         121  CCGCGG               CCGC^GG
SfoI           31  GGCGCC               GGC^GCC
StuI         1109  AGGCCT               AGG^CCT
TfiI         1147  GATTC                G^A[AT]TC
Tsp509I       521  AATT                 ^AATT
XmnI          205  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AlwNI         857  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1212  CAGCCACTG           
AvaI          166  CTCGGG               C^[CT]CG[GA]G
             1197  CTCGGG              
BalI          596  TGGCCA               TGG^CCA
             1170  TGGCCA              
BcnI          764  CCGGG                CC^[GC]GG
              894  CCCGG               
BsmAI         436  GAGAC                GTCTC(1/5)
              979  GTCTC               
BsmFI         125  GGGAC                GGGAC(10/14)
              790  GGGAC               
BsoBI         166  CTCGGG               C^[CT]CG[GA]G
             1197  CTCGGG              
BsrFI         607  ACCGGC               [GA]^CCGG[CT]
             1244  GCCGGC              
BstEII         75  GGTCACC              G^GT[ACGT]ACC
              990  GGTGACC             
BstPI          75  GGTCACC              G^GT[ACGT]ACC
              990  GGTGACC             
Cfr10I        607  ACCGGC               [GA]^CCGG[CT]
             1244  GCCGGC              
DsaI          121  CCGCGG               C^C[GA][CT]GG
              200  CCACGG              
EcoO65I        75  GGTCACC              G^GT[ACGT]ACC
              990  GGTGACC             
EcoT14I       585  CCAAGG               C^C[AT][AT]GG
              956  CCAAGG              
FauI           71  GCGGG                CCCGC(4/6)
              123  GCGGG               
HinfI         531  GACTC                G^A[ACGT]TC
             1147  GATTC               
HphI          990  GGTGA                GGTGA(8/7)
               77  TCACC               
MluNI         596  TGGCCA               TGG^CCA
             1170  TGGCCA              
MscI          596  TGGCCA               TGG^CCA
             1170  TGGCCA              
MseI          191  TTAA                 T^TAA
              926  TTAA                
NciI          764  CCGGG                CC^[GC]GG
              894  CCCGG               
SfaNI        1227  GATGC                GCATC(5/9)
               56  GCATC               
StyI          585  CCAAGG               C^C[AT][AT]GG
              956  CCAAGG              
Tru9I         191  TTAA                 T^TAA
              926  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AccII       AciI        AluI        AspHI       AvaII       
BanI        BanII       BbvI        BmyI        BpmI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EcoNI       EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HpaII       
ItaI        MaeIII      MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NdeII       NlaIII      NlaIV       PpuMI       PstI        
PvuII       SacI        Sau3AI      Sau96I      ScrFI       
SfcI        SmlI        TseI        Tsp45I      TspRI       
XcmI        
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