Restriction map for pF1KSDA1427
 (Linear) (Six-base) MAPSORT of: KIAA1427.seq  Check: 6285  from: 1  to: 1278



KIAA1427.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      88     339    1278 
   Size:         88     251     939

  Fragments arranged by size: 

                 939     251      88
 
AccI GT'mk_AC

Cuts at:      0     310    1278 
   Size:        310     968
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     821     850    1039    1278 
   Size:        821      29     189     239

  Fragments arranged by size: 

                 821     239     189      29
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1178    1278 
   Size:       1178     100
 
AlwNI CAG_nnn'CTG

Cuts at:      0     862    1217    1278 
   Size:        862     355      61

  Fragments arranged by size: 

                 862     355      61
 
AvaI C'yCGr_G

Cuts at:      0     166    1197    1278 
   Size:        166    1031      81

  Fragments arranged by size: 

                1031     166      81
 
BanI G'GyrC_C

Cuts at:      0      12      31    1262    1278 
   Size:         12      19    1231      16

  Fragments arranged by size: 

                1231      19      16      12
 
BanII G_rGCy'C

Cuts at:      0     323     701     773     965    1204    1278 
   Size:        323     378      72     192     239      74

  Fragments arranged by size: 

                 378     323     239     192      74      72
 
BbeI G_GCGC'C

Cuts at:      0      35    1278 
   Size:         35    1243
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     838    1278 
   Size:        838     440
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     991    1025    1278 
   Size:        991      34     253

  Fragments arranged by size: 

                 991     253      34
 
BclI T'GATC_A

Cuts at:      0    1076    1278 
   Size:       1076     202
 
BlpI GC'TnA_GC

Cuts at:      0    1043    1278 
   Size:       1043     235
 
Bme1580I G_kGCm'C

Cuts at:      0      46     225    1278 
   Size:         46     179    1053

  Fragments arranged by size: 

                1053     179      46
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     810    1227    1278 
   Size:        810     417      51

  Fragments arranged by size: 

                 810     417      51
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     257     884    1098    1278 
   Size:        163      94     627     214     180

  Fragments arranged by size: 

                 627     214     180     163      94
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     349     747    1278 
   Size:        214     135     398     531

  Fragments arranged by size: 

                 531     398     214     135
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     430    1278 
   Size:        430     848
 
BsaHI Gr'CG_yC

Cuts at:      0      32    1278 
   Size:         32    1246
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     730     760    1278 
   Size:        730      30     518

  Fragments arranged by size: 

                 730     518      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     217     331     397     679     706     709    1237    1278 
   Size:        217     114      66     282      27       3     528      41

  Fragments arranged by size: 

                 528     282     217     114      66      41      27       3
            
 
BseYI C'CCAG_C

Cuts at:      0      27      39     157    1134    1278 
   Size:         27      12     118     977     144

  Fragments arranged by size: 

                 977     144     118      27      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     311     634    1174    1255    1278 
   Size:        158     153     323     540      81      23

  Fragments arranged by size: 

                 540     323     158     153      81      23
 
BsiEI CG_ry'CG

Cuts at:      0     741    1278 
   Size:        741     537
 
BsiHKAI G_wGCw'C

Cuts at:      0     680     701     773     965    1278 
   Size:        680      21      72     192     313

  Fragments arranged by size: 

                 680     313     192      72      21
 
Bsp1286I G_dGCh'C

Cuts at:      0      46     225     323     680     701     773     965    1204 
   Size:         46     179      98     357      21      72     192     239

Cuts at:   1204    1278 
   Size:         74

  Fragments arranged by size: 

                 357     239     192     179      98      74      72      46
                  21
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      88     339     363     742    1109    1278 
   Size:         88     251      24     379     367     169

  Fragments arranged by size: 

                 379     367     251     169      88      24
 
BsrFI r'CCGG_y

Cuts at:      0     607    1244    1278 
   Size:        607     637      34

  Fragments arranged by size: 

                 637     607      34
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1269    1278 
   Size:       1269       9
 
BstEII G'GTnAC_C

Cuts at:      0      75     990    1278 
   Size:         75     915     288

  Fragments arranged by size: 

                 915     288      75
 
BtgI C'CryG_G

Cuts at:      0     121     200    1278 
   Size:        121      79    1078

  Fragments arranged by size: 

                1078     121      79
 
BtsI GCAGTG_nn'

Cuts at:      0     323    1173    1278 
   Size:        323     850     105

  Fragments arranged by size: 

                 850     323     105
 
DraIII CAC_nnn'GTG

Cuts at:      0     759    1278 
   Size:        759     519
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     580    1278 
   Size:        580     698
 
EaeI y'GGCC_r

Cuts at:      0     596     761    1170    1278 
   Size:        596     165     409     108

  Fragments arranged by size: 

                 596     409     165     108
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     703     763    1278 
   Size:        703      60     515

  Fragments arranged by size: 

                 703     515      60
 
EcoICRI GAG'CTC

Cuts at:      0     699     771     963    1278 
   Size:        699      72     192     315

  Fragments arranged by size: 

                 699     315     192      72
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     257     821     850     884    1039    1098    1278 
   Size:        163      94     564      29      34     155      59     180

  Fragments arranged by size: 

                 564     180     163     155      94      59      34      29
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     145     688     970    1105    1278 
   Size:        145     543     282     135     173

  Fragments arranged by size: 

                 543     282     173     145     135
 
EcoO109I rG'GnC_Cy

Cuts at:      0     132     474     669     731     870    1278 
   Size:        132     342     195      62     139     408

  Fragments arranged by size: 

                 408     342     195     139     132      62
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     177    1011    1278 
   Size:        177     834     267

  Fragments arranged by size: 

                 834     267     177
 
HaeII r_GCGC'y

Cuts at:      0      35    1278 
   Size:         35    1243
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     730     762     778     810    1278 
   Size:        730      32      16      32     468

  Fragments arranged by size: 

                 730     468      32      32      16
 
KasI G'GCGC_C

Cuts at:      0      31    1278 
   Size:         31    1247
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     445    1278 
   Size:        445     833
 
MscI TGG'CCA

Cuts at:      0     598    1172    1278 
   Size:        598     574     106

  Fragments arranged by size: 

                 598     574     106
 
MspA1I CmG'CkG

Cuts at:      0     123     262     648    1269    1278 
   Size:        123     139     386     621       9

  Fragments arranged by size: 

                 621     386     139     123       9
 
NaeI GCC'GGC

Cuts at:      0    1246    1278 
   Size:       1246      32
 
NarI GG'CG_CC

Cuts at:      0      32    1278 
   Size:         32    1246
 
NgoMIV G'CCGG_C

Cuts at:      0    1244    1278 
   Size:       1244      34
 
NspI r_CATG'y

Cuts at:      0      99    1278 
   Size:         99    1179
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     373     405     928     960    1278 
   Size:        373      32     523      32     318

  Fragments arranged by size: 

                 523     373     318      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     132     731     870    1278 
   Size:        132     599     139     408

  Fragments arranged by size: 

                 599     408     139     132
 
PstI C_TGCA'G

Cuts at:      0     858    1109    1180    1278 
   Size:        858     251      71      98

  Fragments arranged by size: 

                 858     251      98      71
 
PvuII CAG'CTG

Cuts at:      0     262     648    1269    1278 
   Size:        262     386     621       9

  Fragments arranged by size: 

                 621     386     262       9
 
SacI G_AGCT'C

Cuts at:      0     701     773     965    1278 
   Size:        701      72     192     313

  Fragments arranged by size: 

                 701     313     192      72
 
SacII CC_GC'GG

Cuts at:      0     124    1278 
   Size:        124    1154
 
SfcI C'TryA_G

Cuts at:      0     854    1105    1176    1278 
   Size:        854     251      71     102

  Fragments arranged by size: 

                 854     251     102      71
 
SfoI GGC'GCC

Cuts at:      0      33    1278 
   Size:         33    1245
 
SmlI C'TyrA_G

Cuts at:      0     229     364     726    1278 
   Size:        229     135     362     552

  Fragments arranged by size: 

                 552     362     229     135
 
StuI AGG'CCT

Cuts at:      0    1111    1278 
   Size:       1111     167
 
StyI C'CwwG_G

Cuts at:      0     585     956    1278 
   Size:        585     371     322

  Fragments arranged by size: 

                 585     371     322
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     909     963    1122    1278 
   Size:        909      54     159     156

  Fragments arranged by size: 

                 909     159     156      54
 
XmnI GAAnn'nnTTC

Cuts at:      0     209    1278 
   Size:        209    1069
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AlfI    AlwNI     AvaI     BanI    BanII
     BbeI     BbsI     BcgI     BclI     BlpI Bme1580I     BmrI     BpmI
    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspMI    BsrFI   BstAPI   BstEII     BtgI     BtsI
   DraIII     DrdI     EaeI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FalI    HaeII    Hin4I     KasI     MmeI     MscI   MspA1I     NaeI
     NarI   NgoMIV     NspI     PpiI    PpuMI     PstI    PvuII     SacI
    SacII     SfcI     SfoI     SmlI     StuI     StyI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BbvCI    BciVI    BfrBI     BglI    BglII
    BmgBI     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaWI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI     EagI     EarI    EcoRI    EcoRV     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MslI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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