################################## # NO CUT ENZYMES ( 40 enzymes) # ################################## AccIII AclI AflII AgeI AscI AseI AsnI AvrII BbeI BfrI BlnI BseAI BsiWI BspEI BstBI CpoI DraI FseI KasI MfeI MluI MroI MunI NarI NdeI NheI NruI NspV PinAI PmeI PshAI PshBI Psp1406I RsrII SfoI SfuI SgrAI SnaBI SspI SwaI ################################### # ONE CUT ENZYMES ( 26 enzymes) # ################################### Acc65I 6570 GGTACC G^GTACC Asp718I 6570 GGTACC G^GTACC BbrPI 2909 CACGTG CAC^GTG BciVI 8628 GGATAC GTATCC(6/5) BssHII 9120 GCGCGC G^CGCGC Bst1107I 8007 GTATAC GTA^TAC BstZ17I 8007 GTATAC GTA^TAC EcoRV 7966 GATATC GAT^ATC EcoT22I 9291 ATGCAT ATGCA^T HindIII 411 AAGCTT A^AGCTT HpaI 12465 GTTAAC GTT^AAC KpnI 6570 GGTACC GGTAC^C KspI 7063 CCGCGG CCGC^GG NotI 7769 GCGGCCGC GC^GGCCGC NsiI 9291 ATGCAT ATGCA^T PacI 1936 TTAATTAA TTAAT^TAA PaeR7I 1590 CTCGAG C^TCGAG PmaCI 2909 CACGTG CAC^GTG PmlI 2909 CACGTG CAC^GTG PvuI 9610 CGATCG CGAT^CG SacII 7063 CCGCGG CCGC^GG SalI 3507 GTCGAC G^TCGAC SfiI 4245 GGCCATCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 12069 ACTAGT A^CTAGT XbaI 1290 TCTAGA T^CTAGA XhoI 1590 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AatII 5637 GACGTC GACGT^C 11968 GACGTC AccI 3507 GTCGAC GT^[AC][GT]AC 8007 GTATAC AocI 43 CCTCAGG CC^T[ACGT]AGG 7317 CCTGAGG BsaWI 4702 ACCGGA [AT]^CCGG[AT] 10686 ACCGGA BsmBI 9403 GAGACG CGTCTC(1/5) 11881 GAGACG BspLU11I 4118 ACATGT A^CATGT 5834 ACATGT Bsu36I 43 CCTCAGG CC^T[ACGT]AGG 7317 CCTGAGG EagI 4040 CGGCCG C^GGCCG 7770 CGGCCG EclXI 4040 CGGCCG C^GGCCG 7770 CGGCCG Eco52I 4040 CGGCCG C^GGCCG 7770 CGGCCG Eco81I 43 CCTCAGG CC^T[ACGT]AGG 7317 CCTGAGG ######################################### # 3 OR MORE CUT ENZYMES ( 181 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AhdI AluI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI ApoI Asp700I AspEI AspHI AspI AvaI AvaII AviII BalI BamHI BanI BanII BbsI BbvCI BbvI BcgI BclI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I ClaI DdeI DpnII DraII DraIII DrdI DsaI EaeI Eam1105I EarI Eco47III EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I SbfI ScaI ScrFI SexAI SfaNI SfcI SmaI SmlI SphI Sse8387I SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI