Restriction map for pF1KSDA1388
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AhdI        AocI        ApaI        
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvrII       BalI        BcgI        
BciVI       BclI        BfrI        BlnI        BsaBI       
BsaI        BseAI       BsiWI       BsmBI       BsmFI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BsrDI       BsrGI       BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DrdI        Eam1105I    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HincII      HindII      
HpaI        KpnI        MamI        MfeI        MluNI       
MroI        MscI        MseI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SapI        ScaI        
SfiI        SfuI        SmaI        SnaBI       SpeI        
SphI        SspBI       SspI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AcyI         1717  GGCGCC               G[GA]^CG[CT]C
AgeI          673  ACCGGT               A^CCGGT
AlwNI         283  CAGTGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1646  AGCGCT               AGC^GCT
AvaII         984  GGACC                G^G[AT]CC
AviII         778  TGCGCA               TGC^GCA
BamHI         665  GGATCC               G^GATCC
BbeI         1717  GGCGCC               GGCGC^C
BbrPI        1171  CACGTG               CAC^GTG
BbsI          792  GAAGAC               GAAGAC(2/6)
BfaI         1654  CTAG                 C^TAG
BglII         332  AGATCT               A^GATCT
BlpI          379  GCTGAGC              GC^T[ACGT]AGC
BpmI          358  CTGGAG               CTGGAG(16/14)
Bpu1102I      379  GCTGAGC              GC^T[ACGT]AGC
BpuAI         792  GAAGAC               GAAGAC(2/6)
BsaAI        1171  CACGTG               [CT]AC^GT[GA]
BsaHI        1717  GGCGCC               G[GA]^CG[CT]C
BsaWI         673  ACCGGT               [AT]^CCGG[AT]
BsiEI        1268  CGGCCG               CG[GA][CT]^CG
BspLU11I      827  ACATGT               A^CATGT
BstF5I        153  CATCC                GGATG(2/0)
CelII         379  GCTGAGC              GC^T[ACGT]AGC
DraII         222  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EaeI         1268  CGGCCG               [CT]^GGCC[GA]
EagI         1268  CGGCCG               C^GGCCG
EclXI        1268  CGGCCG               C^GGCCG
Eco47III     1646  AGCGCT               AGC^GCT
Eco52I       1268  CGGCCG               C^GGCCG
EcoO109I      222  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I      1046  CCATGG               C^C[AT][AT]GG
FokI          153  CATCC                GGATG(9/13)
FspI          778  TGCGCA               TGC^GCA
Hin1I        1717  GGCGCC               G[GA]^CG[CT]C
HindIII       711  AAGCTT               A^AGCTT
KasI         1717  GGCGCC               G^GCGCC
KspI         1703  CCGCGG               CCGC^GG
MaeI         1654  CTAG                 C^TAG
MluI         1490  ACGCGT               A^CGCGT
NarI         1717  GGCGCC               GG^CGCC
NcoI         1046  CCATGG               C^CATGG
NspI          827  ACATGT               [GA]CATG^[CT]
PaeR7I       1183  CTCGAG               C^TCGAG
PflMI         282  CCAGTGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         673  ACCGGT               A^CCGGT
PmaCI        1171  CACGTG               CAC^GTG
PmlI         1171  CACGTG               CAC^GTG
SacI          646  GAGCTC               GAGCT^C
SacII        1703  CCGCGG               CCGC^GG
SbfI          201  CCTGCAGG             CCTGCA^GG
SfoI         1717  GGCGCC               GGC^GCC
Sse8387I      201  CCTGCAGG             CCTGCA^GG
StyI         1046  CCATGG               C^C[AT][AT]GG
TaqI         1184  TCGA                 T^CGA
TthHB8I      1184  TCGA                 T^CGA
Van91I        282  CCAGTGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          347  CCACTGACCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1183  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AvaI          840  CTCGGG               C^[CT]CG[GA]G
             1183  CTCGAG              
BanI         1508  GGCACC               G^G[CT][GA]CC
             1717  GGCGCC              
BanII         646  GAGCTC               G[GA]GC[CT]^C
             1105  GAGCCC              
BbvCI          61  CCTCAGC              CCTCAGC(-5/-2)
              508  GCTGAGG             
BseRI        1404  CTCCTC               GAGGAG(10/8)
             1102  GAGGAG              
BsmAI        1186  GAGAC                GTCTC(1/5)
              326  GTCTC               
BsoBI         840  CTCGGG               C^[CT]CG[GA]G
             1183  CTCGAG              
BssHII       1314  GCGCGC               G^CGCGC
             1682  GCGCGC              
BstYI         332  AGATCT               [GA]^GATC[CT]
              665  GGATCC              
DsaI         1046  CCATGG               C^C[GA][CT]GG
             1703  CCGCGG              
EarI         1192  CTCTTC               CTCTTC(1/4)
             1407  CTCTTC              
HinfI         892  GAGTC                G^A[ACGT]TC
             1188  GACTC               
Ksp632I      1192  CTCTTC               CTCTTC(1/4)
             1407  CTCTTC              
MflI          332  AGATCT               [GA]^GATC[CT]
              665  GGATCC              
PleI         1188  GACTC                GAGTC(4/5)
              892  GAGTC               
XhoII         332  AGATCT               [GA]^GATC[CT]
              665  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AluI        
Alw44I      AlwI        ApaLI       Asp700I     AspHI       
BbvI        BcnI        BglI        BmyI        BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstAPI      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraIII      
EcoRII      FauI        Fnu4HI      HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SexAI       SfaNI       
SfcI        SgrAI       SmlI        StuI        TseI        
Tsp45I      TspRI       XmnI        
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