Restriction map for pF1KSDA1388
 (Linear) (Six-base) MAPSORT of: KIAA1388.seq  Check: 4448  from: 1  to: 1746



KIAA1388.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1163    1746 
   Size:       1163     583
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     699    1746 
   Size:        699    1047
 
AfeI AGC'GCT

Cuts at:      0    1648    1746 
   Size:       1648      98
 
AflIII A'CryG_T

Cuts at:      0     827     869    1490    1746 
   Size:        827      42     621     256

  Fragments arranged by size: 

                 827     621     256      42
 
AgeI A'CCGG_T

Cuts at:      0     673    1746 
   Size:        673    1073
 
AleI CACnn'nnGTG

Cuts at:      0     674    1458    1746 
   Size:        674     784     288

  Fragments arranged by size: 

                 784     674     288
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     175    1709    1746 
   Size:        175    1534      37

  Fragments arranged by size: 

                1534     175      37
 
AlwNI CAG_nnn'CTG

Cuts at:      0     288    1746 
   Size:        288    1458
 
ApaLI G'TGCA_C

Cuts at:      0     315     693     736     918    1174    1603    1746 
   Size:        315     378      43     182     256     429     143

  Fragments arranged by size: 

                 429     378     315     256     182     143      43
 
AvaI C'yCGr_G

Cuts at:      0     840    1183    1746 
   Size:        840     343     563

  Fragments arranged by size: 

                 840     563     343
 
BamHI G'GATC_C

Cuts at:      0     665    1746 
   Size:        665    1081
 
BanI G'GyrC_C

Cuts at:      0    1508    1717    1746 
   Size:       1508     209      29

  Fragments arranged by size: 

                1508     209      29
 
BanII G_rGCy'C

Cuts at:      0     650    1109    1746 
   Size:        650     459     637

  Fragments arranged by size: 

                 650     637     459
 
BbeI G_GCGC'C

Cuts at:      0    1721    1746 
   Size:       1721      25
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     799    1746 
   Size:        799     947
 
BbvCI CC'TCA_GC

Cuts at:      0      62     509    1746 
   Size:         62     447    1237

  Fragments arranged by size: 

                1237     447      62
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1049    1217    1262    1705    1746 
   Size:       1049     168      45     443      41

  Fragments arranged by size: 

                1049     443     168      45      41
 
BglII A'GATC_T

Cuts at:      0     332    1746 
   Size:        332    1414
 
BlpI GC'TnA_GC

Cuts at:      0     380    1746 
   Size:        380    1366
 
Bme1580I G_kGCm'C

Cuts at:      0     118     319     697     740     922    1045    1178    1204 
   Size:        118     201     378      43     182     123     133      26

Cuts at:   1204    1291    1465    1549    1607    1746 
   Size:         87     174      84      58     139

  Fragments arranged by size: 

                 378     201     182     174     139     133     123     118
                  87      84      58      43      26
 
BmgBI CAC'GTC

Cuts at:      0    1659    1746 
   Size:       1659      87
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379    1746 
   Size:        379    1367
 
Bpu10I CC'TnA_GC

Cuts at:      0      62     509    1746 
   Size:         62     447    1237

  Fragments arranged by size: 

                1237     447      62
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313    1441    1746 
   Size:        313    1128     305

  Fragments arranged by size: 

                1128     313     305
 
BsaAI yAC'GTr

Cuts at:      0    1173    1746 
   Size:       1173     573
 
BsaHI Gr'CG_yC

Cuts at:      0    1718    1746 
   Size:       1718      28
 
BsaWI w'CCGG_w

Cuts at:      0     673    1746 
   Size:        673    1073
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      93     123     352     382     439     469    1746 
   Size:         93      30     229      30      57      30    1277

  Fragments arranged by size: 

                1277     229      93      57      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1117    1395    1746 
   Size:       1117     278     351

  Fragments arranged by size: 

                1117     351     278
 
BseYI C'CCAG_C

Cuts at:      0    1147    1746 
   Size:       1147     599
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394    1362    1441    1746 
   Size:        394     968      79     305

  Fragments arranged by size: 

                 968     394     305      79
 
BsiEI CG_ry'CG

Cuts at:      0    1271    1746 
   Size:       1271     475
 
BsiHKAI G_wGCw'C

Cuts at:      0     101     281     319     365     650     697     740     851 
   Size:        101     180      38      46     285      47      43     111

Cuts at:    851     922    1178    1607    1679    1746 
   Size:         71     256     429      72      67

  Fragments arranged by size: 

                 429     285     256     180     111     101      72      71
                  67      47      46      43      38
 
Bsp1286I G_dGCh'C

Cuts at:      0     101     118     281     319     365     650     697     740 
   Size:        101      17     163      38      46     285      47      43

Cuts at:    740     851     922    1045    1109    1178    1204    1291    1465 
   Size:        111      71     123      64      69      26      87     174

Cuts at:   1465    1549    1607    1679    1746 
   Size:         84      58      72      67

  Fragments arranged by size: 

                 285     174     163     123     111     101      87      84
                  72      71      69      67      64      58      47      46
                  43      38      26      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     362     995    1163    1508    1746 
   Size:        362     633     168     345     238

  Fragments arranged by size: 

                 633     362     345     238     168
 
BsrBI CCG'CTC

Cuts at:      0     305     663     765     809     936    1020    1275    1746 
   Size:        305     358     102      44     127      84     255     471

  Fragments arranged by size: 

                 471     358     305     255     127     102      84      44
            
 
BsrFI r'CCGG_y

Cuts at:      0     673    1082    1511    1746 
   Size:        673     409     429     235

  Fragments arranged by size: 

                 673     429     409     235
 
BssHII G'CGCG_C

Cuts at:      0    1314    1682    1746 
   Size:       1314     368      64

  Fragments arranged by size: 

                1314     368      64
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     105     735     872    1385    1746 
   Size:        105     630     137     513     361

  Fragments arranged by size: 

                 630     513     361     137     105
 
BstYI r'GATC_y

Cuts at:      0     332     665    1746 
   Size:        332     333    1081

  Fragments arranged by size: 

                1081     333     332
 
BtgI C'CryG_G

Cuts at:      0    1046    1703    1746 
   Size:       1046     657      43

  Fragments arranged by size: 

                1046     657      43
 
DraIII CAC_nnn'GTG

Cuts at:      0     323     701     735     819    1746 
   Size:        323     378      34      84     927

  Fragments arranged by size: 

                 927     378     323      84      34
 
EaeI y'GGCC_r

Cuts at:      0    1268    1746 
   Size:       1268     478
 
EagI C'GGCC_G

Cuts at:      0    1268    1746 
   Size:       1268     478
 
EarI CTCTTCn'nnn_

Cuts at:      0    1198    1413    1746 
   Size:       1198     215     333

  Fragments arranged by size: 

                1198     333     215
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     136    1746 
   Size:        136    1610
 
EcoICRI GAG'CTC

Cuts at:      0     648    1746 
   Size:        648    1098
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     699    1746 
   Size:        379     320    1047

  Fragments arranged by size: 

                1047     379     320
 
EcoO109I rG'GnC_Cy

Cuts at:      0     223    1746 
   Size:        223    1523
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     435    1746 
   Size:        435    1311
 
FspI TGC'GCA

Cuts at:      0     780    1746 
   Size:        780     966
 
HaeII r_GCGC'y

Cuts at:      0    1016    1650    1721    1746 
   Size:       1016     634      71      25

  Fragments arranged by size: 

                1016     634      71      25
 
HindIII A'AGCT_T

Cuts at:      0     711    1746 
   Size:        711    1035
 
KasI G'GCGC_C

Cuts at:      0    1717    1746 
   Size:       1717      29
 
MluI A'CGCG_T

Cuts at:      0    1490    1746 
   Size:       1490     256
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     623     878    1746 
   Size:        623     255     868

  Fragments arranged by size: 

                 868     623     255
 
MslI CAynn'nnrTG

Cuts at:      0     312     674    1458    1746 
   Size:        312     362     784     288

  Fragments arranged by size: 

                 784     362     312     288
 
MspA1I CmG'CkG

Cuts at:      0     508     517     608     863     947    1341    1430    1506 
   Size:        508       9      91     255      84     394      89      76

Cuts at:   1506    1575    1705    1711    1746 
   Size:         69     130       6      35

  Fragments arranged by size: 

                 508     394     255     130      91      89      84      76
                  69      35       9       6
 
NarI GG'CG_CC

Cuts at:      0    1718    1746 
   Size:       1718      28
 
NcoI C'CATG_G

Cuts at:      0    1046    1746 
   Size:       1046     700
 
NspI r_CATG'y

Cuts at:      0     831    1746 
   Size:        831     915
 
PasI CC'CwG_GG

Cuts at:      0     132    1746 
   Size:        132    1614
 
PciI A'CATG_T

Cuts at:      0     827    1746 
   Size:        827     919
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     288    1746 
   Size:        288    1458
 
PmlI CAC'GTG

Cuts at:      0    1173    1746 
   Size:       1173     573
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1606    1638    1746 
   Size:       1606      32     108

  Fragments arranged by size: 

                1606     108      32
 
PstI C_TGCA'G

Cuts at:      0     164     182     206     911    1331    1505    1746 
   Size:        164      18      24     705     420     174     241

  Fragments arranged by size: 

                 705     420     241     174     164      24      18
 
PvuII CAG'CTG

Cuts at:      0     508     608    1341    1746 
   Size:        508     100     733     405

  Fragments arranged by size: 

                 733     508     405     100
 
SacI G_AGCT'C

Cuts at:      0     650    1746 
   Size:        650    1096
 
SacII CC_GC'GG

Cuts at:      0    1706    1746 
   Size:       1706      40
 
SbfI CC_TGCA'GG

Cuts at:      0     206    1746 
   Size:        206    1540
 
SexAI A'CCwGG_T

Cuts at:      0     731     815    1724    1746 
   Size:        731      84     909      22

  Fragments arranged by size: 

                 909     731      84      22
 
SfcI C'TryA_G

Cuts at:      0     160     178     202     603     907    1327    1501    1746 
   Size:        160      18      24     401     304     420     174     245

  Fragments arranged by size: 

                 420     401     304     245     174     160      24      18
            
 
SfoI GGC'GCC

Cuts at:      0    1719    1746 
   Size:       1719      27
 
SgrAI Cr'CCGG_yG

Cuts at:      0     673    1082    1511    1746 
   Size:        673     409     429     235

  Fragments arranged by size: 

                 673     429     409     235
 
SmlI C'TyrA_G

Cuts at:      0     328    1183    1456    1746 
   Size:        328     855     273     290

  Fragments arranged by size: 

                 855     328     290     273
 
StuI AGG'CCT

Cuts at:      0     967    1306    1564    1746 
   Size:        967     339     258     182

  Fragments arranged by size: 

                 967     339     258     182
 
StyI C'CwwG_G

Cuts at:      0    1046    1746 
   Size:       1046     700
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1114    1746 
   Size:       1114     632
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      93     125     352     384     439     471    1746 
   Size:         93      32     227      32      55      32    1275

  Fragments arranged by size: 

                1275     227      93      55      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     354    1746 
   Size:        354    1392
 
XhoI C'TCGA_G

Cuts at:      0    1183    1746 
   Size:       1183     563
 
XmnI GAAnn'nnTTC

Cuts at:      0     391     796     967    1480    1746 
   Size:        391     405     171     513     266

  Fragments arranged by size: 

                 513     405     391     266     171
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AgeI     AleI     AlfI    AlwNI
    ApaLI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BglI    BglII     BlpI Bme1580I    BmgBI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI   BssHII   BstAPI    BstYI
     BtgI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I     FalI     FspI    HaeII  HindIII     KasI     MluI     MmeI
     MslI   MspA1I     NarI     NcoI     NspI     PasI     PciI    PflMI
     PmlI     PpiI     PstI    PvuII     SacI    SacII     SbfI    SexAI
     SfcI     SfoI    SgrAI     SmlI     StuI     StyI    TaqII     TstI
     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AhdI     AloI     ApaI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BcgI    BciVI
     BclI    BfrBI     BmrI     BmtI     BplI     BsaI    BsaBI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI    EcoNI    EcoRI    EcoRV     FseI    FspAI    Hin4I
   HincII     HpaI     KpnI     MfeI     MscI     NaeI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PfoI     PmeI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SalI
    SanDI     SapI     ScaI     SfiI     SgfI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TatI  Tth111I     XbaI     XmaI
     ZraI
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