Restriction map for pF1KSDA1382
 (Linear) (Six-base) MAPSORT of: KIAA1382.seq  Check: 1962  from: 1  to: 1518



KIAA1382.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AflIII A'CryG_T

Cuts at:      0    1056    1518 
   Size:       1056     462
 
AlwNI CAG_nnn'CTG

Cuts at:      0     451    1518 
   Size:        451    1067
 
ApoI r'AATT_y

Cuts at:      0     165     193     639     718    1518 
   Size:        165      28     446      79     800

  Fragments arranged by size: 

                 800     446     165      79      28
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      50     376    1518 
   Size:         50     326    1142

  Fragments arranged by size: 

                1142     326      50
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1030    1518 
   Size:       1030     488
 
BsaBI GATnn'nnATC

Cuts at:      0     131    1518 
   Size:        131    1387
 
BsaHI Gr'CG_yC

Cuts at:      0    1233    1518 
   Size:       1233     285
 
BsaWI w'CCGG_w

Cuts at:      0    1181    1459    1518 
   Size:       1181     278      59

  Fragments arranged by size: 

                1181     278      59
 
BspEI T'CCGG_A

Cuts at:      0    1181    1518 
   Size:       1181     337
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     813    1518 
   Size:        813     705
 
BsrDI GCAATG_nn'

Cuts at:      0     249    1518 
   Size:        249    1269
 
BsrGI T'GTAC_A

Cuts at:      0    1407    1518 
   Size:       1407     111
 
BstYI r'GATC_y

Cuts at:      0     132    1518 
   Size:        132    1386
 
BtgI C'CryG_G

Cuts at:      0     284    1518 
   Size:        284    1234
 
BtsI GCAGTG_nn'

Cuts at:      0     264    1518 
   Size:        264    1254
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     330    1518 
   Size:        330    1188
 
EaeI y'GGCC_r

Cuts at:      0    1512    1518 
   Size:       1512       6
 
EarI CTCTTCn'nnn_

Cuts at:      0     502    1092    1518 
   Size:        502     590     426

  Fragments arranged by size: 

                 590     502     426
 
EcoRV GAT'ATC

Cuts at:      0    1308    1518 
   Size:       1308     210
 
HindIII A'AGCT_T

Cuts at:      0     439    1518 
   Size:        439    1079
 
MfeI C'AATT_G

Cuts at:      0     406    1518 
   Size:        406    1112
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     414    1518 
   Size:         89     325    1104

  Fragments arranged by size: 

                1104     325      89
 
MscI TGG'CCA

Cuts at:      0    1514    1518 
   Size:       1514       4
 
MslI CAynn'nnrTG

Cuts at:      0    1249    1518 
   Size:       1249     269
 
NcoI C'CATG_G

Cuts at:      0     284    1518 
   Size:        284    1234
 
NspI r_CATG'y

Cuts at:      0    1060    1518 
   Size:       1060     458
 
PciI A'CATG_T

Cuts at:      0    1056    1518 
   Size:       1056     462
 
PsiI TTA'TAA

Cuts at:      0     313    1518 
   Size:        313    1205
 
PstI C_TGCA'G

Cuts at:      0    1335    1518 
   Size:       1335     183
 
SfcI C'TryA_G

Cuts at:      0      57    1331    1518 
   Size:         57    1274     187

  Fragments arranged by size: 

                1274     187      57
 
SspI AAT'ATT

Cuts at:      0     338    1518 
   Size:        338    1180
 
StyI C'CwwG_G

Cuts at:      0     267     284    1518 
   Size:        267      17    1234

  Fragments arranged by size: 

                1234     267      17
 
TatI w'GTAC_w

Cuts at:      0    1407    1446    1518 
   Size:       1407      39      72

  Fragments arranged by size: 

                1407      72      39
 
XmnI GAAnn'nnTTC

Cuts at:      0    1073    1518 
   Size:       1073     445
 

 Enzymes that do cut:

   AflIII    AlwNI     ApoI     BbsI    BciVI    BsaBI    BsaHI    BsaWI
    BspEI    BspMI    BsrDI    BsrGI    BstYI     BtgI     BtsI     DrdI
     EaeI     EarI    EcoRV  HindIII     MfeI     MmeI     MscI     MslI
     NcoI     NspI     PciI     PsiI     PstI     SfcI     SspI     StyI
     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspHI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     EagI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII     HpaI     KasI     KpnI     MluI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI
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