Restriction map for pF1KSDA1349
 (Linear) (Six-base) MAPSORT of: KIAA1349.seq  Check: 6866  from: 1  to: 2217



KIAA1349.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     386     959    1379    1887    2055    2139    2217 
   Size:         71     315     573     420     508     168      84      78

  Fragments arranged by size: 

                 573     508     420     315     168      84      78      71
            
 
AflIII A'CryG_T

Cuts at:      0    1833    2217 
   Size:       1833     384
 
AleI CACnn'nnGTG

Cuts at:      0     854    2217 
   Size:        854    1363
 
ApaLI G'TGCA_C

Cuts at:      0    1675    2217 
   Size:       1675     542
 
ApoI r'AATT_y

Cuts at:      0     309     321     327     677     761    1161    1181    1433 
   Size:        309      12       6     350      84     400      20     252

Cuts at:   1433    1517    2021    2217 
   Size:         84     504     196

  Fragments arranged by size: 

                 504     400     350     309     252     196      84      84
                  20      12       6
 
AseI AT'TA_AT

Cuts at:      0    1235    2217 
   Size:       1235     982
 
BanII G_rGCy'C

Cuts at:      0       8    2217 
   Size:          8    2209
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     211     934    2217 
   Size:        211     723    1283

  Fragments arranged by size: 

                1283     723     211
 
BfrBI ATG'CAT

Cuts at:      0    1269    2109    2217 
   Size:       1269     840     108

  Fragments arranged by size: 

                1269     840     108
 
Bme1580I G_kGCm'C

Cuts at:      0     741    1679    2217 
   Size:        741     938     538

  Fragments arranged by size: 

                 938     741     538
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1115    2039    2217 
   Size:       1115     924     178

  Fragments arranged by size: 

                1115     924     178
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     623     791    1043    1463    1547    1631    1715    1799 
   Size:        623     168     252     420      84      84      84      84

Cuts at:   1799    1967    2135    2217 
   Size:        168     168      82

  Fragments arranged by size: 

                 623     420     252     168     168     168      84      84
                  84      84      82
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184    1486    2121    2217 
   Size:        184    1302     635      96

  Fragments arranged by size: 

                1302     635     184      96
 
BsaBI GATnn'nnATC

Cuts at:      0     237    2217 
   Size:        237    1980
 
BsiHKAI G_wGCw'C

Cuts at:      0    1679    2217 
   Size:       1679     538
 
BsmI GAATG_Cn'

Cuts at:      0      30     802    1144    1228    1271    1480    2217 
   Size:         30     772     342      84      43     209     737

  Fragments arranged by size: 

                 772     737     342     209      84      43      30
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     741    1679    2217 
   Size:          8     733     938     538

  Fragments arranged by size: 

                 938     733     538       8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      21    2217 
   Size:         21    2196
 
BsrDI GCAATG_nn'

Cuts at:      0     263     487     644    1386    1722    2217 
   Size:        263     224     157     742     336     495

  Fragments arranged by size: 

                 742     495     336     263     224     157
 
BsrGI T'GTAC_A

Cuts at:      0     918    1254    1422    1590    2094    2178    2217 
   Size:        918     336     168     168     504      84      39

  Fragments arranged by size: 

                 918     504     336     168     168      84      39
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1140    2217 
   Size:       1140    1077
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     948     983    1368    1403    1704    1739    2217 
   Size:        948      35     385      35     301      35     478

  Fragments arranged by size: 

                 948     478     385     301      35      35      35
 
DraI TTT'AAA

Cuts at:      0     326    1794    2217 
   Size:        326    1468     423

  Fragments arranged by size: 

                1468     423     326
 
EarI CTCTTCn'nnn_

Cuts at:      0     269     935    1355    2217 
   Size:        269     666     420     862

  Fragments arranged by size: 

                 862     666     420     269
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     386     623     791     959    1043    1379    1463 
   Size:         71     315     237     168     168      84     336      84

Cuts at:   1463    1547    1631    1715    1799    1887    1967    2055    2135 
   Size:         84      84      84      84      88      80      88      80

Cuts at:   2135    2139    2217 
   Size:          4      78

  Fragments arranged by size: 

                 336     315     237     168     168      88      88      84
                  84      84      84      84      84      80      80      78
                  71       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1905    2217 
   Size:       1905     312
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1601    2039    2217 
   Size:       1601     438     178

  Fragments arranged by size: 

                1601     438     178
 
EcoRI G'AATT_C

Cuts at:      0     677     761    1181    1433    2021    2217 
   Size:        677      84     420     252     588     196

  Fragments arranged by size: 

                 677     588     420     252     196      84
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     416    2217 
   Size:        416    1801
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     476     508    2217 
   Size:        476      32    1709

  Fragments arranged by size: 

                1709     476      32
 
HindIII A'AGCT_T

Cuts at:      0     806    2217 
   Size:        806    1411
 
MslI CAynn'nnrTG

Cuts at:      0     854     954    1458    1489    1626    1794    2217 
   Size:        854     100     504      31     137     168     423

  Fragments arranged by size: 

                 854     504     423     168     137     100      31
 
NsiI A_TGCA'T

Cuts at:      0    1271    2111    2217 
   Size:       1271     840     106

  Fragments arranged by size: 

                1271     840     106
 
NspI r_CATG'y

Cuts at:      0     464    1837    2217 
   Size:        464    1373     380

  Fragments arranged by size: 

                1373     464     380
 
PciI A'CATG_T

Cuts at:      0    1833    2217 
   Size:       1833     384
 
PpuMI rG'GwC_Cy

Cuts at:      0    1601    2217 
   Size:       1601     616
 
PsiI TTA'TAA

Cuts at:      0    1289    2217 
   Size:       1289     928
 
SapI GCTCTTCn'nnn_

Cuts at:      0     269    2217 
   Size:        269    1948
 
SfcI C'TryA_G

Cuts at:      0    1062    2217 
   Size:       1062    1155
 
SmlI C'TyrA_G

Cuts at:      0     199    1501    2100    2217 
   Size:        199    1302     599     117

  Fragments arranged by size: 

                1302     599     199     117
 
SpeI A'CTAG_T

Cuts at:      0     214    2217 
   Size:        214    2003
 
StuI AGG'CCT

Cuts at:      0     472     640    1312    2217 
   Size:        472     168     672     905

  Fragments arranged by size: 

                 905     672     472     168
 
SwaI ATTT'AAAT

Cuts at:      0     326    1794    2217 
   Size:        326    1468     423

  Fragments arranged by size: 

                1468     423     326
 
TatI w'GTAC_w

Cuts at:      0     457     918    1254    1294    1422    1462    1590    2094 
   Size:        457     461     336      40     128      40     128     504

Cuts at:   2094    2178    2217 
   Size:         84      39

  Fragments arranged by size: 

                 504     461     457     336     128     128      84      40
                  40      39
 
XmnI GAAnn'nnTTC

Cuts at:      0     242     278     556    1071    1144    1228    1648    2217 
   Size:        242      36     278     515      73      84     420     569

  Fragments arranged by size: 

                 569     515     420     278     242      84      73      36
            
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    ApaLI     ApoI     AseI    BanII     BbsI
    BfrBI Bme1580I     BmrI     BpmI    BpuEI    BsaBI  BsiHKAI     BsmI
 Bsp1286I    BspMI    BsrDI    BsrGI    BtgZI    CspCI     DraI     EarI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    Hin4I  HindIII     MslI
     NsiI     NspI     PciI    PpuMI     PsiI     SapI     SfcI     SmlI
     SpeI     StuI     SwaI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI     BtsI     ClaI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI     StyI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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