Restriction map for pF1KSDA1344
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BclI        BcnI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaI        
BsiEI       BsiWI       BsiYI       BslI        BsmAI       
BsmBI       BsoBI       BspHI       BspLU11I    BsrBI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        CpoI        DraII       
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HhaI        HinP1I      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MspA1I      MunI        MvnI        NaeI        
NarI        NciI        NgoMIV      NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    StuI        SwaI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccIII       1842  TCCGGA               T^CCGGA
AcyI          884  GGCGTC               G[GA]^CG[CT]C
AlwNI        1130  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         514  GAGCAC               G[AT]GC[AT]^C
AvaII        1096  GGTCC                G^G[AT]CC
BalI         2097  TGGCCA               TGG^CCA
BciVI        1261  GTATCC               GTATCC(6/5)
BglII        1458  AGATCT               A^GATCT
BmyI          514  GAGCAC               G[AGT]GC[ACT]^C
BpmI         2012  CTCCAG               CTGGAG(16/14)
BsaHI         884  GGCGTC               G[GA]^CG[CT]C
BsaWI        1842  TCCGGA               [AT]^CCGG[AT]
BseAI        1842  TCCGGA               T^CCGGA
BseRI        2222  CTCCTC               GAGGAG(10/8)
BsiHKAI       514  GAGCAC               G[AT]GC[AT]^C
Bsp1286I      514  GAGCAC               G[AGT]GC[ACT]^C
Bsp1407I     1406  TGTACA               T^GTACA
BspDI        1512  ATCGAT               AT^CGAT
BspEI        1842  TCCGGA               T^CCGGA
BspMI        2202  ACCTGC               ACCTGC(4/8)
BsrFI         535  GCCGGT               [GA]^CCGG[CT]
BsrGI        1406  TGTACA               T^GTACA
Cfr10I        535  GCCGGT               [GA]^CCGG[CT]
Cfr13I       1096  GGTCC                G^G[ACGT]CC
ClaI         1512  ATCGAT               AT^CGAT
DraI         2416  TTTAAA               TTT^AAA
DrdI         1592  GACTATTTAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1265  CCATGG               C^C[GA][CT]GG
EaeI         2097  TGGCCA               [CT]^GGCC[GA]
EcoRI         337  GAATTC               G^AATTC
FauI         2034  GCGGG                CCCGC(4/6)
HaeIII       2098  GGCC                 GG^CC
HgaI          885  GCGTC                GACGC(5/10)
Hin1I         884  GGCGTC               G[GA]^CG[CT]C
MaeIII       1381  GTTAC                ^GT[ACGT]AC
MluNI        2097  TGGCCA               TGG^CCA
MroI         1842  TCCGGA               T^CCGGA
MscI         2097  TGGCCA               TGG^CCA
NcoI         1265  CCATGG               C^CATGG
NdeI          488  CATATG               CA^TATG
NheI         1783  GCTAGC               G^CTAGC
NlaIV        1450  GGAACC               GG[ACGT]^[ACGT]CC
SapI          394  GCTCTTC              GCTCTTC(1/4)
Sau96I       1096  GGTCC                G^G[ACGT]CC
ScaI         1233  AGTACT               AGT^ACT
SpeI          651  ACTAGT               A^CTAGT
SspBI        1406  TGTACA               T^GTACA
TaqI         1513  TCGA                 T^CGA
TthHB8I      1513  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AciI         2261  CCGC                 CCGC(-3/-1)
             2034  GCGG                
BbvI         1717  GCTGC                GCAGC(8/12)
             1737  GCTGC               
BsaJI         239  CCAAGG               C^C[ACGT][ACGT]GG
             1265  CCATGG              
BsgI         1346  GTGCAG               GTGCAG(16/14)
             2317  GTGCAG              
BsmI          366  GAATGC               GAATGC(1/-1)
             2088  GCATTC              
BsrDI         116  CATTGC               GCAATG(2/0)
              672  GCAATG              
EcoT14I       239  CCAAGG               C^C[AT][AT]GG
             1265  CCATGG              
HincII        753  GTTGAC               GT[CT]^[GA]AC
             1704  GTCAAC              
HindII        753  GTTGAC               GT[CT]^[GA]AC
             1704  GTCAAC              
HphI         2079  GGTGA                GGTGA(8/7)
              720  TCACC               
MaeII        2274  ACGT                 A^CGT
             2442  ACGT                
MslI         1398  CATAAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2089  CATTCAGGTG          
MwoI          133  GCCTCTTTAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              370  GCCATTGTCGC         
NspI           62  ACATGC               [GA]CATG^[CT]
             1791  ACATGC              
PleI          925  GACTC                GAGTC(4/5)
               93  GAGTC               
PstI          214  CTGCAG               CTGCA^G
              866  CTGCAG              
SfcI          214  CTGCAG               C^T[GA][CT]AG
              866  CTGCAG              
SmlI          947  CTCAAG               C^T[CT][GA]AG
             2207  CTCAAG              
StyI          239  CCAAGG               C^C[AT][AT]GG
             1265  CCATGG              
TseI         1717  GCTGC                G^C[AT]GC
             1737  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
Asp700I     BbsI        BfaI        BpuAI       BsmFI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       DdeI        DpnII       EarI        EcoRII      
Fnu4HI      FokI        HapII       HinfI       HpaII       
ItaI        Ksp632I     MaeI        MboI        MboII       
MflI        MnlI        MseI        MspI        MvaI        
NdeII       NlaIII      RsaI        Sau3AI      ScrFI       
SfaNI       SspI        TfiI        Tru9I       Tsp509I     
TspRI       XhoII       XmnI        
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