Restriction map for pF1KSDA1344
 (Linear) (Six-base) MAPSORT of: KIAA1344.seq  Check: 4516  from: 1  to: 2475



KIAA1344.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424     779     788    1703    1867    2102    2179    2475 
   Size:        424     355       9     915     164     235      77     296

  Fragments arranged by size: 

                 915     424     355     296     235     164      77       9
            
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     343    2475 
   Size:        343    2132
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1135    2475 
   Size:       1135    1340
 
ApoI r'AATT_y

Cuts at:      0     227     337     997    1387    1468    1673    2475 
   Size:        227     110     660     390      81     205     802

  Fragments arranged by size: 

                 802     660     390     227     205     110      81
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     440     880     957    1085    1717    2475 
   Size:        440     440      77     128     632     758

  Fragments arranged by size: 

                 758     632     440     440     128      77
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1272    2475 
   Size:       1272    1203
 
BglII A'GATC_T

Cuts at:      0    1458    2475 
   Size:       1458    1017
 
BmgBI CAC'GTC

Cuts at:      0    2443    2475 
   Size:       2443      32
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     814    1420    1862    2475 
   Size:        814     606     442     613

  Fragments arranged by size: 

                 814     613     606     442
 
BmtI G_CTAG'C

Cuts at:      0    1787    2475 
   Size:       1787     688
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     928    2475 
   Size:        928    1547
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1997    2475 
   Size:       1997     478
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     932    2192    2475 
   Size:        932    1260     283

  Fragments arranged by size: 

                1260     932     283
 
BsaHI Gr'CG_yC

Cuts at:      0     885    2475 
   Size:        885    1590
 
BsaWI w'CCGG_w

Cuts at:      0    1842    2475 
   Size:       1842     633
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     977    1007    2475 
   Size:        977      30    1468

  Fragments arranged by size: 

                1468     977      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2213    2475 
   Size:       2213     262
 
BseYI C'CCAG_C

Cuts at:      0     668    2475 
   Size:        668    1807
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1367    2338    2475 
   Size:       1367     971     137

  Fragments arranged by size: 

                1367     971     137
 
BsiHKAI G_wGCw'C

Cuts at:      0     518    2475 
   Size:        518    1957
 
BsmI GAATG_Cn'

Cuts at:      0     372    2088    2475 
   Size:        372    1716     387

  Fragments arranged by size: 

                1716     387     372
 
Bsp1286I G_dGCh'C

Cuts at:      0     518    2475 
   Size:        518    1957
 
BspEI T'CCGG_A

Cuts at:      0    1842    2475 
   Size:       1842     633
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2211    2475 
   Size:       2211     264
 
BsrDI GCAATG_nn'

Cuts at:      0     115     679    2475 
   Size:        115     564    1796

  Fragments arranged by size: 

                1796     564     115
 
BsrFI r'CCGG_y

Cuts at:      0     535    2475 
   Size:        535    1940
 
BsrGI T'GTAC_A

Cuts at:      0    1406    2475 
   Size:       1406    1069
 
BstYI r'GATC_y

Cuts at:      0      32     771    1458    2475 
   Size:         32     739     687    1017

  Fragments arranged by size: 

                1017     739     687      32
 
BtgI C'CryG_G

Cuts at:      0    1265    2475 
   Size:       1265    1210
 
ClaI AT'CG_AT

Cuts at:      0    1513    2475 
   Size:       1513     962
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     564     599    2475 
   Size:        564      35    1876

  Fragments arranged by size: 

                1876     564      35
 
DraI TTT'AAA

Cuts at:      0    2418    2475 
   Size:       2418      57
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1598    2475 
   Size:       1598     877
 
EaeI y'GGCC_r

Cuts at:      0    2097    2475 
   Size:       2097     378
 
EarI CTCTTCn'nnn_

Cuts at:      0     401     974    1147    1243    2072    2231    2475 
   Size:        401     573     173      96     829     159     244

  Fragments arranged by size: 

                 829     573     401     244     173     159      96
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424     779     788    1703    1867    1997    2102    2179 
   Size:        424     355       9     915     164     130     105      77

Cuts at:   2179    2475 
   Size:        296

  Fragments arranged by size: 

                 915     424     355     296     164     130     105      77
                   9
 
EcoRI G'AATT_C

Cuts at:      0     337    2475 
   Size:        337    2138
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     944    2161    2475 
   Size:        944    1217     314

  Fragments arranged by size: 

                1217     944     314
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      17      49    1479    1511    1649    1681    2364    2396 
   Size:         17      32    1430      32     138      32     683      32

Cuts at:   2396    2475 
   Size:         79

  Fragments arranged by size: 

                1430     683     138      79      32      32      32      32
                  17
 
HincII GTy'rAC

Cuts at:      0     755    1706    2475 
   Size:        755     951     769

  Fragments arranged by size: 

                 951     769     755
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     825    1627    2475 
   Size:        825     802     848

  Fragments arranged by size: 

                 848     825     802
 
MscI TGG'CCA

Cuts at:      0    2099    2475 
   Size:       2099     376
 
MslI CAynn'nnrTG

Cuts at:      0    1402    2093    2475 
   Size:       1402     691     382

  Fragments arranged by size: 

                1402     691     382
 
NcoI C'CATG_G

Cuts at:      0    1265    2475 
   Size:       1265    1210
 
NdeI CA'TA_TG

Cuts at:      0     489    2475 
   Size:        489    1986
 
NheI G'CTAG_C

Cuts at:      0    1783    2475 
   Size:       1783     692
 
NspI r_CATG'y

Cuts at:      0      66    1795    2475 
   Size:         66    1729     680

  Fragments arranged by size: 

                1729     680      66
 
PstI C_TGCA'G

Cuts at:      0     218     870    2475 
   Size:        218     652    1605

  Fragments arranged by size: 

                1605     652     218
 
SapI GCTCTTCn'nnn_

Cuts at:      0     401    2475 
   Size:        401    2074
 
ScaI AGT'ACT

Cuts at:      0    1235    2475 
   Size:       1235    1240
 
SfcI C'TryA_G

Cuts at:      0     214     866    2475 
   Size:        214     652    1609

  Fragments arranged by size: 

                1609     652     214
 
SmlI C'TyrA_G

Cuts at:      0     947    2207    2475 
   Size:        947    1260     268

  Fragments arranged by size: 

                1260     947     268
 
SpeI A'CTAG_T

Cuts at:      0     651    2475 
   Size:        651    1824
 
SspI AAT'ATT

Cuts at:      0     106     477    1534    1946    2475 
   Size:        106     371    1057     412     529

  Fragments arranged by size: 

                1057     529     412     371     106
 
StyI C'CwwG_G

Cuts at:      0     239    1265    2475 
   Size:        239    1026    1210

  Fragments arranged by size: 

                1210    1026     239
 
TatI w'GTAC_w

Cuts at:      0     112    1233    1366    1406    2475 
   Size:        112    1121     133      40    1069

  Fragments arranged by size: 

                1121    1069     133     112      40
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     977    1009    1908    1940    2475 
   Size:        977      32     899      32     535

  Fragments arranged by size: 

                 977     899     535      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     437    1677    1838    1862    2475 
   Size:        437    1240     161      24     613

  Fragments arranged by size: 

                1240     613     437     161      24
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApoI     BbsI    BciVI    BglII    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI
    BsrDI    BsrFI    BsrGI    BstYI     BtgI     ClaI    CspCI     DraI
     DrdI     EaeI     EarI  Eco57MI    EcoRI     FalI    Hin4I   HincII
     MmeI     MscI     MslI     NcoI     NdeI     NheI     NspI     PstI
     SapI     ScaI     SfcI     SmlI     SpeI     SspI     StyI     TatI
     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI Bme1580I   Bpu10I
     BsaI    BsaAI    BsaBI    BsiEI    BsiWI    BsmBI    BspHI    BsrBI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI   DraIII     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NaeI     NarI   NgoMIV     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI
     SrfI     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI
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