Restriction map for pF1KSDA1341
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AviII       AvrII       
BalI        BbeI        BbvCI       BcgI        BciVI       
BclI        BfrI        BlnI        BlpI        Bpu1102I    
BsaI        BseAI       BsgI        BsiEI       BsiWI       
BsmAI       BsmBI       BsmI        Bsp1407I    BspEI       
BspLU11I    BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
EcoT14I     FbaI        FseI        FspI        HaeII       
HhaI        HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        MluI        MluNI       
MroI        MscI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PinAI       PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SphI        Sse8387I    SspBI       StuI        StyI        
SwaI        XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AcyI            7  GACGCC               G[GA]^CG[CT]C
AflIII        588  ACACGT               A^C[GA][CT]GT
Asp700I       853  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         391  GAGCTC               G[AT]GC[AT]^C
AspI         1010  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           28  CCCGGG               C^[CT]CG[GA]G
BamHI         525  GGATCC               G^GATCC
BanI           24  GGTGCC               G^G[CT][GA]CC
BanII         391  GAGCTC               G[GA]GC[CT]^C
BbrPI         983  CACGTG               CAC^GTG
BglI           10  GCCAACAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         351  AGATCT               A^GATCT
BsaHI           7  GACGCC               G[GA]^CG[CT]C
BsaWI        1433  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       391  GAGCTC               G[AT]GC[AT]^C
BsoBI          28  CCCGGG               C^[CT]CG[GA]G
BspDI        1495  ATCGAT               AT^CGAT
BspHI         939  TCATGA               T^CATGA
BspMI          56  ACCTGC               ACCTGC(4/8)
BsrFI         682  GCCGGT               [GA]^CCGG[CT]
Cfr10I        682  GCCGGT               [GA]^CCGG[CT]
ClaI         1495  ATCGAT               AT^CGAT
DdeI          496  CTTAG                C^T[ACGT]AG
EaeI         1194  TGGCCG               [CT]^GGCC[GA]
EcoT22I       907  ATGCAT               ATGCA^T
HgaI            7  GACGC                GACGC(5/10)
Hin1I           7  GACGCC               G[GA]^CG[CT]C
MfeI          848  CAATTG               C^AATTG
MunI          848  CAATTG               C^AATTG
NdeI          323  CATATG               CA^TATG
NsiI          907  ATGCAT               ATGCA^T
NspI          909  GCATGT               [GA]CATG^[CT]
PflFI        1010  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1105  CCAGGTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1708  GACTC                GAGTC(4/5)
PmaCI         983  CACGTG               CAC^GTG
PmlI          983  CACGTG               CAC^GTG
PvuII        1721  CAGCTG               CAG^CTG
RcaI          939  TCATGA               T^CATGA
SacI          391  GAGCTC               GAGCT^C
SexAI        1464  ACCAGGT              A^CC[AT]GGT
SmaI           28  CCCGGG               CCC^GGG
SpeI         1228  ACTAGT               A^CTAGT
SspI          514  AATATT               AAT^ATT
Tth111I      1010  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1105  CCAGGTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI          28  CCCGGG               C^CCGGG
XmaI           28  CCCGGG               C^CCGGG
XmnI          853  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI          686  GTAGAC               GT^[AC][GT]AC
             1298  GTAGAC              
AccII          86  CGCG                 CG^CG
              105  CGCG                
AfaI          948  GTAC                 GT^AC
             1212  GTAC                
BbsI          503  GAAGAC               GAAGAC(2/6)
              796  GAAGAC              
BfaI          472  CTAG                 C^TAG
             1229  CTAG                
BpmI          272  CTGGAG               CTGGAG(16/14)
             1409  CTGGAG              
BpuAI         503  GAAGAC               GAAGAC(2/6)
              796  GAAGAC              
BsaAI         385  TACGTG               [CT]AC^GT[GA]
              983  CACGTG              
BsaBI        1498  GATATGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1511  GATTTTTATC          
BseRI        1133  GAGGAG               GAGGAG(10/8)
             1187  GAGGAG              
BstUI          86  CGCG                 CG^CG
              105  CGCG                
EarI          184  GAAGAG               CTCTTC(1/4)
              943  GAAGAG              
EcoRI         261  GAATTC               G^AATTC
              439  GAATTC              
HphI          373  GGTGA                GGTGA(8/7)
             1258  GGTGA               
Ksp632I       184  GAAGAG               CTCTTC(1/4)
              943  GAAGAG              
KspI           85  CCGCGG               CCGC^GG
              104  CCGCGG              
MaeI          472  CTAG                 C^TAG
             1229  CTAG                
MamI         1498  GATATGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1511  GATTTTTATC          
MslI          999  CATTGGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1494  CATCGATATG          
MvnI           86  CGCG                 CG^CG
              105  CGCG                
PstI           58  CTGCAG               CTGCA^G
             1734  CTGCAG              
RsaI          948  GTAC                 GT^AC
             1212  GTAC                
SacII          85  CCGCGG               CCGC^GG
              104  CCGCGG              
SfaNI         403  GATGC                GCATC(5/9)
             1493  GCATC               
Tsp45I        832  GTCAC                ^GT[GC]AC
             1405  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
AvaII       BbvI        BcnI        BmyI        BsaJI       
BsiYI       BslI        BsmFI       Bsp1286I    BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DpnII       DsaI        EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       ItaI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       SfcI        TaqI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     XhoII       
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