Restriction map for pF1KSDA1341
 (Linear) (Six-base) MAPSORT of: KIAA1341.seq  Check: 8848  from: 1  to: 1800



KIAA1341.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      65    1800 
   Size:         65    1735
 
AccI GT'mk_AC

Cuts at:      0     687    1299    1800 
   Size:        687     612     501

  Fragments arranged by size: 

                 687     612     501
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     680    1800 
   Size:        680    1120
 
AflIII A'CryG_T

Cuts at:      0     588    1800 
   Size:        588    1212
 
ApoI r'AATT_y

Cuts at:      0     261     439     454     618    1071    1624    1800 
   Size:        261     178      15     164     453     553     176

  Fragments arranged by size: 

                 553     453     261     178     176     164      15
 
AvaI C'yCGr_G

Cuts at:      0      28    1800 
   Size:         28    1772
 
BamHI G'GATC_C

Cuts at:      0     525    1800 
   Size:        525    1275
 
BanI G'GyrC_C

Cuts at:      0      24    1800 
   Size:         24    1776
 
BanII G_rGCy'C

Cuts at:      0     395    1800 
   Size:        395    1405
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     510     803    1800 
   Size:        510     293     997

  Fragments arranged by size: 

                 997     510     293
 
BfrBI ATG'CAT

Cuts at:      0     909    1800 
   Size:        909     891
 
BglI GCCn_nnn'nGGC

Cuts at:      0      16    1800 
   Size:         16    1784
 
BglII A'GATC_T

Cuts at:      0     351    1800 
   Size:        351    1449
 
Bme1580I G_kGCm'C

Cuts at:      0      29     271     829    1800 
   Size:         29     242     558     971

  Fragments arranged by size: 

                 971     558     242      29
 
BmgBI CAC'GTC

Cuts at:      0     591    1255    1800 
   Size:        591     664     545

  Fragments arranged by size: 

                 664     591     545
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293    1430    1800 
   Size:        293    1137     370

  Fragments arranged by size: 

                1137     370     293
 
BsaAI yAC'GTr

Cuts at:      0     387     985    1800 
   Size:        387     598     815

  Fragments arranged by size: 

                 815     598     387
 
BsaBI GATnn'nnATC

Cuts at:      0    1502    1515    1800 
   Size:       1502      13     285

  Fragments arranged by size: 

                1502     285      13
 
BsaHI Gr'CG_yC

Cuts at:      0       8    1800 
   Size:          8    1792
 
BsaWI w'CCGG_w

Cuts at:      0    1433    1800 
   Size:       1433     367
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     575     605    1800 
   Size:        575      30    1195

  Fragments arranged by size: 

                1195     575      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1148    1202    1800 
   Size:       1148      54     598

  Fragments arranged by size: 

                1148     598      54
 
BsiHKAI G_wGCw'C

Cuts at:      0     395    1800 
   Size:        395    1405
 
Bsp1286I G_dGCh'C

Cuts at:      0      29     271     395     829    1800 
   Size:         29     242     124     434     971

  Fragments arranged by size: 

                 971     434     242     124      29
 
BspHI T'CATG_A

Cuts at:      0     939    1800 
   Size:        939     861
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      65    1800 
   Size:         65    1735
 
BsrDI GCAATG_nn'

Cuts at:      0     553    1169    1319    1800 
   Size:        553     616     150     481

  Fragments arranged by size: 

                 616     553     481     150
 
BsrFI r'CCGG_y

Cuts at:      0     682    1800 
   Size:        682    1118
 
BstYI r'GATC_y

Cuts at:      0     351     525    1342    1366    1800 
   Size:        351     174     817      24     434

  Fragments arranged by size: 

                 817     434     351     174      24
 
BtgI C'CryG_G

Cuts at:      0      85     104    1215    1800 
   Size:         85      19    1111     585

  Fragments arranged by size: 

                1111     585      85      19
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1237    1800 
   Size:       1237     563
 
BtsI GCAGTG_nn'

Cuts at:      0    1314    1800 
   Size:       1314     486
 
ClaI AT'CG_AT

Cuts at:      0    1496    1800 
   Size:       1496     304
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     687     722    1800 
   Size:        687      35    1078

  Fragments arranged by size: 

                1078     687      35
 
EaeI y'GGCC_r

Cuts at:      0    1194    1800 
   Size:       1194     606
 
EarI CTCTTCn'nnn_

Cuts at:      0     179     938    1800 
   Size:        179     759     862

  Fragments arranged by size: 

                 862     759     179
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     127    1800 
   Size:         19     108    1673

  Fragments arranged by size: 

                1673     108      19
 
EcoICRI GAG'CTC

Cuts at:      0     393    1800 
   Size:        393    1407
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     680    1430    1800 
   Size:        293     387     750     370

  Fragments arranged by size: 

                 750     387     370     293
 
EcoRI G'AATT_C

Cuts at:      0     261     439    1800 
   Size:        261     178    1361

  Fragments arranged by size: 

                1361     261     178
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     575     589     607     621     913     945    1800 
   Size:        575      14      18      14     292      32     855

  Fragments arranged by size: 

                 855     575     292      32      18      14      14
 
MfeI C'AATT_G

Cuts at:      0     848    1800 
   Size:        848     952
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     715    1800 
   Size:        715    1085
 
MslI CAynn'nnrTG

Cuts at:      0    1003    1498    1800 
   Size:       1003     495     302

  Fragments arranged by size: 

                1003     495     302
 
MspA1I CmG'CkG

Cuts at:      0      87     106    1723    1800 
   Size:         87      19    1617      77

  Fragments arranged by size: 

                1617      87      77      19
 
NdeI CA'TA_TG

Cuts at:      0     324    1800 
   Size:        324    1476
 
NsiI A_TGCA'T

Cuts at:      0     911    1800 
   Size:        911     889
 
NspI r_CATG'y

Cuts at:      0     913    1800 
   Size:        913     887
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1111    1800 
   Size:       1111     689
 
PfoI T'CCnGG_A

Cuts at:      0     579    1800 
   Size:        579    1221
 
PmlI CAC'GTG

Cuts at:      0     985    1800 
   Size:        985     815
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     678     710    1800 
   Size:        678      32    1090

  Fragments arranged by size: 

                1090     678      32
 
PstI C_TGCA'G

Cuts at:      0      62    1738    1800 
   Size:         62    1676      62

  Fragments arranged by size: 

                1676      62      62
 
PvuII CAG'CTG

Cuts at:      0    1723    1800 
   Size:       1723      77
 
SacI G_AGCT'C

Cuts at:      0     395    1800 
   Size:        395    1405
 
SacII CC_GC'GG

Cuts at:      0      88     107    1800 
   Size:         88      19    1693

  Fragments arranged by size: 

                1693      88      19
 
SexAI A'CCwGG_T

Cuts at:      0    1464    1800 
   Size:       1464     336
 
SfcI C'TryA_G

Cuts at:      0      58     212    1734    1800 
   Size:         58     154    1522      66

  Fragments arranged by size: 

                1522     154      66      58
 
SmaI CCC'GGG

Cuts at:      0      30    1800 
   Size:         30    1770
 
SpeI A'CTAG_T

Cuts at:      0    1228    1800 
   Size:       1228     572
 
SspI AAT'ATT

Cuts at:      0     516    1800 
   Size:        516    1284
 
Tth111I GACn'n_nGTC

Cuts at:      0    1013    1800 
   Size:       1013     787
 
XmaI C'CCGG_G

Cuts at:      0      28    1800 
   Size:         28    1772
 
XmnI GAAnn'nnTTC

Cuts at:      0     857    1800 
   Size:        857     943
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     ApoI     AvaI    BamHI     BanI
    BanII     BbsI    BfrBI     BglI    BglII Bme1580I    BmgBI     BpmI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI  BsiHKAI Bsp1286I
    BspHI    BspMI    BsrDI    BsrFI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoRI
    Hin4I     MfeI     MmeI     MslI   MspA1I     NdeI     NsiI     NspI
    PflMI     PfoI     PmlI     PsrI     PstI    PvuII     SacI    SacII
    SexAI     SfcI     SmaI     SpeI     SspI  Tth111I     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI    BbvCI     BcgI    BciVI     BclI     BlpI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     DraI
   DraIII     DrdI     EagI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MscI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PvuI    RsrII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SphI     SrfI     StuI     StyI     SwaI    TaqII     TatI     TstI
     XbaI     XcmI     XhoI     ZraI
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