Restriction map for pF1KSDA1337
 (Linear) (Six-base) MAPSORT of: KIAA1337.seq  Check: 8310  from: 1  to: 4176



KIAA1337.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3063    4176 
   Size:       3063    1113
 
AatII G_ACGT'C

Cuts at:      0    2410    4176 
   Size:       2410    1766
 
AccI GT'mk_AC

Cuts at:      0    1309    1430    2531    2665    3113    3373    4176 
   Size:       1309     121    1101     134     448     260     803

  Fragments arranged by size: 

                1309    1101     803     448     260     134     121
 
Acc65I G'GTAC_C

Cuts at:      0     692    4176 
   Size:        692    3484
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427    1189    2742    3209    4176 
   Size:        427     762    1553     467     967

  Fragments arranged by size: 

                1553     967     762     467     427
 
AflII C'TTAA_G

Cuts at:      0     376    4176 
   Size:        376    3800
 
AflIII A'CryG_T

Cuts at:      0    1937    2057    4176 
   Size:       1937     120    2119

  Fragments arranged by size: 

                2119    1937     120
 
AleI CACnn'nnGTG

Cuts at:      0    1285    3979    4176 
   Size:       1285    2694     197

  Fragments arranged by size: 

                2694    1285     197
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2725    3295    4033    4176 
   Size:       2725     570     738     143

  Fragments arranged by size: 

                2725     738     570     143
 
AlwNI CAG_nnn'CTG

Cuts at:      0     474     675    1080    1412    2145    2304    3219    3450 
   Size:        474     201     405     332     733     159     915     231

Cuts at:   3450    4176 
   Size:        726

  Fragments arranged by size: 

                 915     733     726     474     405     332     231     201
                 159
 
ApaI G_GGCC'C

Cuts at:      0      93     107    1984    2268    3213    4104    4176 
   Size:         93      14    1877     284     945     891      72

  Fragments arranged by size: 

                1877     945     891     284      93      72      14
 
AvaI C'yCGr_G

Cuts at:      0     506    1951    2080    3099    3283    3300    3463    4176 
   Size:        506    1445     129    1019     184      17     163     713

  Fragments arranged by size: 

                1445    1019     713     506     184     163     129      17
            
 
BamHI G'GATC_C

Cuts at:      0     391    4176 
   Size:        391    3785
 
BanI G'GyrC_C

Cuts at:      0     692     754    1860    2485    2598    2853    3149    4032 
   Size:        692      62    1106     625     113     255     296     883

Cuts at:   4032    4081    4114    4176 
   Size:         49      33      62

  Fragments arranged by size: 

                1106     883     692     625     296     255     113      62
                  62      49      33
 
BanII G_rGCy'C

Cuts at:      0      93     107     513     830     985    1099    1177    1876 
   Size:         93      14     406     317     155     114      78     699

Cuts at:   1876    1984    2268    2695    3213    3290    3472    4104    4176 
   Size:        108     284     427     518      77     182     632      72

  Fragments arranged by size: 

                 699     632     518     427     406     317     284     182
                 155     114     108      93      78      77      72      14
            
 
BbeI G_GCGC'C

Cuts at:      0     758    4036    4176 
   Size:        758    3278     140

  Fragments arranged by size: 

                3278     758     140
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     662    1649    1731    1918    3033    4176 
   Size:        662     987      82     187    1115    1143

  Fragments arranged by size: 

                1143    1115     987     662     187      82
 
BbvCI CC'TCA_GC

Cuts at:      0    3637    4176 
   Size:       3637     539
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3156    3190    4176 
   Size:       3156      34     986

  Fragments arranged by size: 

                3156     986      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1543    3009    4176 
   Size:       1543    1466    1167

  Fragments arranged by size: 

                1543    1466    1167
 
BfrBI ATG'CAT

Cuts at:      0    2849    4176 
   Size:       2849    1327
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1297    2263    3592    4176 
   Size:       1297     966    1329     584

  Fragments arranged by size: 

                1329    1297     966     584
 
BglII A'GATC_T

Cuts at:      0    1052    4176 
   Size:       1052    3124
 
BlpI GC'TnA_GC

Cuts at:      0     676    2872    4176 
   Size:        676    2196    1304

  Fragments arranged by size: 

                2196    1304     676
 
Bme1580I G_kGCm'C

Cuts at:      0      93     107     747    1984    2268    2603    2933    3152 
   Size:         93      14     640    1237     284     335     330     219

Cuts at:   3152    3213    3891    3917    4104    4176 
   Size:         61     678      26     187      72

  Fragments arranged by size: 

                1237     678     640     335     330     284     219     187
                  93      72      61      26      14
 
BmgBI CAC'GTC

Cuts at:      0    1938    2058    3087    4176 
   Size:       1938     120    1029    1089

  Fragments arranged by size: 

                1938    1089    1029     120
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     378    2166    2808    3207    4176 
   Size:        378    1788     642     399     969

  Fragments arranged by size: 

                1788     969     642     399     378
 
BmtI G_CTAG'C

Cuts at:      0    2453    4176 
   Size:       2453    1723
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1170    2358    2619    4176 
   Size:       1170    1188     261    1557

  Fragments arranged by size: 

                1557    1188    1170     261
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     649    1123    1866    1888    2038    2065    2434 
   Size:        201     448     474     743      22     150      27     369

Cuts at:   2434    2665    2862    3424    3702    3806    3867    3868    4176 
   Size:        231     197     562     278     104      61       1     308

  Fragments arranged by size: 

                 743     562     474     448     369     308     278     231
                 201     197     150     104      61      27      22       1
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    2326    2433    3637    4176 
   Size:       2326     107    1204     539

  Fragments arranged by size: 

                2326    1204     539     107
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     949    2299    4054    4176 
   Size:        949    1350    1755     122

  Fragments arranged by size: 

                1755    1350     949     122
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     610    1492    3089    4176 
   Size:        610     882    1597    1087

  Fragments arranged by size: 

                1597    1087     882     610
 
BsaAI yAC'GTr

Cuts at:      0    1287    1521    2769    3380    3864    4176 
   Size:       1287     234    1248     611     484     312

  Fragments arranged by size: 

                1287    1248     611     484     312     234
 
BsaHI Gr'CG_yC

Cuts at:      0     755    2407    3056    3329    3818    4033    4176 
   Size:        755    1652     649     273     489     215     143

  Fragments arranged by size: 

                1652     755     649     489     273     215     143
 
BsaWI w'CCGG_w

Cuts at:      0    2402    4176 
   Size:       2402    1774
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     415     445    1726    1756    2524    2554    3223    3253 
   Size:        415      30    1281      30     768      30     669      30

Cuts at:   3253    4176 
   Size:        923

  Fragments arranged by size: 

                1281     923     768     669     415      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58      61      70    3231    3625    3729    4176 
   Size:         58       3       9    3161     394     104     447

  Fragments arranged by size: 

                3161     447     394     104      58       9       3
 
BseYI C'CCAG_C

Cuts at:      0     165     194     220     700     764     978    1006    1241 
   Size:        165      29      26     480      64     214      28     235

Cuts at:   1241    1752    2049    2186    3425    3689    4020    4036    4110 
   Size:        511     297     137    1239     264     331      16      74

Cuts at:   4110    4176 
   Size:         66

  Fragments arranged by size: 

                1239     511     480     331     297     264     235     214
                 165     137      74      66      64      29      28      26
                  16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1906    2134    2152    3367    3559    4176 
   Size:       1906     228      18    1215     192     617

  Fragments arranged by size: 

                1906    1215     617     228     192      18
 
BsiHKAI G_wGCw'C

Cuts at:      0     233     830     965    1177    1209    1876    3506    3635 
   Size:        233     597     135     212      32     667    1630     129

Cuts at:   3635    4130    4176 
   Size:        495      46

  Fragments arranged by size: 

                1630     667     597     495     233     212     135     129
                  46      32
 
BsiWI C'GTAC_G

Cuts at:      0    3841    4176 
   Size:       3841     335
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     430    1928    2048    2214    2947    3885    4176 
   Size:        430    1498     120     166     733     938     291

  Fragments arranged by size: 

                1498     938     733     430     291     166     120
 
Bsp1286I G_dGCh'C

Cuts at:      0      93     107     233     513     747     830     965     985 
   Size:         93      14     126     280     234      83     135      20

Cuts at:    985    1099    1177    1209    1876    1984    2268    2603    2695 
   Size:        114      78      32     667     108     284     335      92

Cuts at:   2695    2933    3152    3213    3290    3472    3506    3635    3891 
   Size:        238     219      61      77     182      34     129     256

Cuts at:   3891    3917    4104    4130    4176 
   Size:         26     187      26      46

  Fragments arranged by size: 

                 667     335     284     280     256     238     234     219
                 187     182     135     129     126     114     108      93
                  92      83      78      77      61      46      34      32
                  26      26      20      14
 
BspEI T'CCGG_A

Cuts at:      0    2402    4176 
   Size:       2402    1774
 
BspHI T'CATG_A

Cuts at:      0    1016    4176 
   Size:       1016    3160
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1947    3063    3302    3428    3505    3785    3803    4176 
   Size:       1947    1116     239     126      77     280      18     373

  Fragments arranged by size: 

                1947    1116     373     280     239     126      77      18
            
 
BsrBI CCG'CTC

Cuts at:      0     491     599    3686    4139    4176 
   Size:        491     108    3087     453      37

  Fragments arranged by size: 

                3087     491     453     108      37
 
BsrDI GCAATG_nn'

Cuts at:      0    1415    4176 
   Size:       1415    2761
 
BsrFI r'CCGG_y

Cuts at:      0     407    2243    2453    2994    4176 
   Size:        407    1836     210     541    1182

  Fragments arranged by size: 

                1836    1182     541     407     210
 
BsrGI T'GTAC_A

Cuts at:      0    2492    4176 
   Size:       2492    1684
 
BssHII G'CGCG_C

Cuts at:      0     777     841    4176 
   Size:        777      64    3335

  Fragments arranged by size: 

                3335     777      64
 
BssSI C'ACGA_G

Cuts at:      0     337     955    4123    4176 
   Size:        337     618    3168      53

  Fragments arranged by size: 

                3168     618     337      53
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     474    1412    2145    2345    3258    3450    3459    3656 
   Size:        474     938     733     200     913     192       9     197

Cuts at:   3656    4176 
   Size:        520

  Fragments arranged by size: 

                 938     913     733     520     474     200     197     192
                   9
 
BstEII G'GTnAC_C

Cuts at:      0    1278    3666    4176 
   Size:       1278    2388     510

  Fragments arranged by size: 

                2388    1278     510
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1638    1768    3091    4176 
   Size:       1638     130    1323    1085

  Fragments arranged by size: 

                1638    1323    1085     130
 
BstYI r'GATC_y

Cuts at:      0     326     391     633     969    1052    1193    1748    3353 
   Size:        326      65     242     336      83     141     555    1605

Cuts at:   3353    4176 
   Size:        823

  Fragments arranged by size: 

                1605     823     555     336     326     242     141      83
                  65
 
Bsu36I CC'TnA_GG

Cuts at:      0    2459    4176 
   Size:       2459    1717
 
BtgI C'CryG_G

Cuts at:      0     254     527     569    2115    2835    3026    3315    3359 
   Size:        254     273      42    1546     720     191     289      44

Cuts at:   3359    3587    3743    3896    4176 
   Size:        228     156     153     280

  Fragments arranged by size: 

                1546     720     289     280     273     254     228     191
                 156     153      44      42
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3159    3888    3918    4176 
   Size:       3159     729      30     258

  Fragments arranged by size: 

                3159     729     258      30
 
BtsI GCAGTG_nn'

Cuts at:      0    1194    3329    3355    4176 
   Size:       1194    2135      26     821

  Fragments arranged by size: 

                2135    1194     821      26
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1882    1917    4176 
   Size:       1882      35    2259

  Fragments arranged by size: 

                2259    1882      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     960    4176 
   Size:        960    3216
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3085    4176 
   Size:       3085    1091
 
EaeI y'GGCC_r

Cuts at:      0     419     728    1109    1289    1298    1562    1808    2118 
   Size:        419     309     381     180       9     264     246     310

Cuts at:   2118    2771    3004    3584    3593    3872    4176 
   Size:        653     233     580       9     279     304

  Fragments arranged by size: 

                 653     580     419     381     310     309     304     279
                 264     246     233     180       9       9
 
EarI CTCTTCn'nnn_

Cuts at:      0     238    1165    1513    2024    2281    2416    2503    3139 
   Size:        238     927     348     511     257     135      87     636

Cuts at:   3139    3925    4176 
   Size:        786     251

  Fragments arranged by size: 

                 927     786     636     511     348     257     251     238
                 135      87
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     417    1099    2239    4176 
   Size:        417     682    1140    1937

  Fragments arranged by size: 

                1937    1140     682     417
 
EcoICRI GAG'CTC

Cuts at:      0     828    1175    1874    4176 
   Size:        828     347     699    2302

  Fragments arranged by size: 

                2302     828     699     347
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     427     649    1123    1189    1866    1888    2038 
   Size:        201     226     222     474      66     677      22     150

Cuts at:   2038    2065    2434    2665    2742    2862    3209    3424    3702 
   Size:         27     369     231      77     120     347     215     278

Cuts at:   3702    3806    3867    3868    4176 
   Size:        104      61       1     308

  Fragments arranged by size: 

                 677     474     369     347     308     278     231     226
                 222     215     201     150     120     104      77      66
                  61      27      22       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     116    2897    3136    3166    3766    4176 
   Size:        116    2781     239      30     600     410

  Fragments arranged by size: 

                2781     600     410     239     116      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0      89     103     104     550    1077    1149    1980    1981 
   Size:         89      14       1     446     527      72     831       1

Cuts at:   1981    2160    2265    2439    3209    4074    4100    4101    4166 
   Size:        179     105     174     770     865      26       1      65

Cuts at:   4166    4176 
   Size:         10

  Fragments arranged by size: 

                 865     831     770     527     446     179     174     105
                  89      72      65      26      14      10       1       1
                   1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1710    2772    2823    2847    4176 
   Size:       1710    1062      51      24    1329

  Fragments arranged by size: 

                1710    1329    1062      51      24
 
FspI TGC'GCA

Cuts at:      0    1186    1366    1657    4176 
   Size:       1186     180     291    2519

  Fragments arranged by size: 

                2519    1186     291     180
 
HaeII r_GCGC'y

Cuts at:      0     376     450     758     882    2333    2626    3548    3834 
   Size:        376      74     308     124    1451     293     922     286

Cuts at:   3834    4036    4176 
   Size:        202     140

  Fragments arranged by size: 

                1451     922     376     308     293     286     202     140
                 124      74
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     415     447    2023    2055    4176 
   Size:        415      32    1576      32    2121

  Fragments arranged by size: 

                2121    1576     415      32      32
 
HincII GTy'rAC

Cuts at:      0    3060    3374    4176 
   Size:       3060     314     802

  Fragments arranged by size: 

                3060     802     314
 
KasI G'GCGC_C

Cuts at:      0     754    4032    4176 
   Size:        754    3278     144

  Fragments arranged by size: 

                3278     754     144
 
KpnI G_GTAC'C

Cuts at:      0     696    4176 
   Size:        696    3480
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1941    2126    4176 
   Size:       1941     185    2050

  Fragments arranged by size: 

                2050    1941     185
 
MscI TGG'CCA

Cuts at:      0    1111    1291    1300    2773    3874    4176 
   Size:       1111     180       9    1473    1101     302

  Fragments arranged by size: 

                1473    1111    1101     302     180       9
 
MslI CAynn'nnrTG

Cuts at:      0     253    1285    1588    2299    3580    3979    4176 
   Size:        253    1032     303     711    1281     399     197

  Fragments arranged by size: 

                1281    1032     711     399     303     253     197
 
MspA1I CmG'CkG

Cuts at:      0     294     465     474     529     571     672     978    1241 
   Size:        294     171       9      55      42     101     306     263

Cuts at:   1241    1412    1493    1653    1889    2117    2136    2186    2527 
   Size:        171      81     160     236     228      19      50     341

Cuts at:   2527    3309    3450    3589    4176 
   Size:        782     141     139     587

  Fragments arranged by size: 

                 782     587     341     306     294     263     236     228
                 171     171     160     141     139     101      81      55
                  50      42      19       9
 
NaeI GCC'GGC

Cuts at:      0    2245    2455    2996    4176 
   Size:       2245     210     541    1180

  Fragments arranged by size: 

                2245    1180     541     210
 
NarI GG'CG_CC

Cuts at:      0     755    4033    4176 
   Size:        755    3278     143

  Fragments arranged by size: 

                3278     755     143
 
NcoI C'CATG_G

Cuts at:      0     254    3026    3359    4176 
   Size:        254    2772     333     817

  Fragments arranged by size: 

                2772     817     333     254
 
NgoMIV G'CCGG_C

Cuts at:      0    2243    2453    2994    4176 
   Size:       2243     210     541    1182

  Fragments arranged by size: 

                2243    1182     541     210
 
NheI G'CTAG_C

Cuts at:      0    2449    4176 
   Size:       2449    1727
 
NsiI A_TGCA'T

Cuts at:      0    2851    4176 
   Size:       2851    1325
 
NspI r_CATG'y

Cuts at:      0    1389    4176 
   Size:       1389    2787
 
PasI CC'CwG_GG

Cuts at:      0     189    1977    2444    4077    4104    4176 
   Size:        189    1788     467    1633      27      72

  Fragments arranged by size: 

                1788    1633     467     189      72      27
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1080    1542    4176 
   Size:       1080     462    2634

  Fragments arranged by size: 

                2634    1080     462
 
PfoI T'CCnGG_A

Cuts at:      0     932    2369    4176 
   Size:        932    1437    1807

  Fragments arranged by size: 

                1807    1437     932
 
PmlI CAC'GTG

Cuts at:      0    1521    3864    4176 
   Size:       1521    2343     312

  Fragments arranged by size: 

                2343    1521     312
 
PpuMI rG'GwC_Cy

Cuts at:      0    1077    2160    2439    4176 
   Size:       1077    1083     279    1737

  Fragments arranged by size: 

                1737    1083    1077     279
 
PspOMI G'GGCC_C

Cuts at:      0      89     103    1980    2264    3209    4100    4176 
   Size:         89      14    1877     284     945     891      76

  Fragments arranged by size: 

                1877     945     891     284      89      76      14
 
PstI C_TGCA'G

Cuts at:      0      29     247     470    1161    1683    1833    2093    2141 
   Size:         29     218     223     691     522     150     260      48

Cuts at:   2141    2393    2474    3254    3455    4136    4176 
   Size:        252      81     780     201     681      40

  Fragments arranged by size: 

                 780     691     681     522     260     252     223     218
                 201     150      81      48      40      29
 
PvuII CAG'CTG

Cuts at:      0     465     474    1412    1493    1653    1889    2136    3309 
   Size:        465       9     938      81     160     236     247    1173

Cuts at:   3309    3450    4176 
   Size:        141     726

  Fragments arranged by size: 

                1173     938     726     465     247     236     160     141
                  81       9
 
SacI G_AGCT'C

Cuts at:      0     830    1177    1876    4176 
   Size:        830     347     699    2300

  Fragments arranged by size: 

                2300     830     699     347
 
SacII CC_GC'GG

Cuts at:      0     530     572    2118    3590    4176 
   Size:        530      42    1546    1472     586

  Fragments arranged by size: 

                1546    1472     586     530      42
 
SalI G'TCGA_C

Cuts at:      0    3372    4176 
   Size:       3372     804
 
SanDI GG'GwC_CC

Cuts at:      0    2439    4176 
   Size:       2439    1737
 
SapI GCTCTTCn'nnn_

Cuts at:      0     238    1165    2024    4176 
   Size:        238     927     859    2152

  Fragments arranged by size: 

                2152     927     859     238
 
SbfI CC_TGCA'GG

Cuts at:      0    2474    4176 
   Size:       2474    1702
 
ScaI AGT'ACT

Cuts at:      0    1540    4176 
   Size:       1540    2636
 
SexAI A'CCwGG_T

Cuts at:      0    3761    4176 
   Size:       3761     415
 
SfcI C'TryA_G

Cuts at:      0      25     243     466    1157    1679    1829    2089    2137 
   Size:         25     218     223     691     522     150     260      48

Cuts at:   2137    2389    2470    2532    2666    3231    3250    3451    4132 
   Size:        252      81      62     134     565      19     201     681

Cuts at:   4132    4176 
   Size:         44

  Fragments arranged by size: 

                 691     681     565     522     260     252     223     218
                 201     150     134      81      62      48      44      25
                  19
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1297    3592    4176 
   Size:       1297    2295     584

  Fragments arranged by size: 

                2295    1297     584
 
SfoI GGC'GCC

Cuts at:      0     756    4034    4176 
   Size:        756    3278     142

  Fragments arranged by size: 

                3278     756     142
 
SmaI CCC'GGG

Cuts at:      0    3101    3302    4176 
   Size:       3101     201     874

  Fragments arranged by size: 

                3101     874     201
 
SmlI C'TyrA_G

Cuts at:      0     376     964    2314    4069    4176 
   Size:        376     588    1350    1755     107

  Fragments arranged by size: 

                1755    1350     588     376     107
 
SpeI A'CTAG_T

Cuts at:      0    3009    4176 
   Size:       3009    1167
 
StuI AGG'CCT

Cuts at:      0     343    3457    4176 
   Size:        343    3114     719

  Fragments arranged by size: 

                3114     719     343
 
StyI C'CwwG_G

Cuts at:      0     254    2566    2720    2777    2933    3026    3359    4176 
   Size:        254    2312     154      57     156      93     333     817

  Fragments arranged by size: 

                2312     817     333     254     156     154      93      57
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     938    1648    2178    3622    4069    4102    4176 
   Size:        938     710     530    1444     447      33      74

  Fragments arranged by size: 

                1444     938     710     530     447      74      33
 
TatI w'GTAC_w

Cuts at:      0    1538    2318    2492    2804    3677    4176 
   Size:       1538     780     174     312     873     499

  Fragments arranged by size: 

                1538     873     780     499     312     174
 
Tth111I GACn'n_nGTC

Cuts at:      0    1597    4176 
   Size:       1597    2579
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     879    1330    1525    1992    3033    3189    4176 
   Size:        879     451     195     467    1041     156     987

  Fragments arranged by size: 

                1041     987     879     467     451     195     156
 
XmaI C'CCGG_G

Cuts at:      0    3099    3300    4176 
   Size:       3099     201     876

  Fragments arranged by size: 

                3099     876     201
 
ZraI GAC'GTC

Cuts at:      0    2408    4176 
   Size:       2408    1768
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI    AflII   AflIII     AleI
     AlfI    AlwNI     ApaI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     DrdI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NsiI
     NspI     PasI    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI
    PvuII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI     SpeI     StuI
     StyI    TaqII     TatI  Tth111I     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AclI     AfeI     AgeI     AhdI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BclI    BsaBI    BsiEI     BsmI
    BstBI  BstZ17I     ClaI     DraI     EagI    EcoRI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     MluI     NdeI     NotI     NruI
     PacI     PciI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SgfI    SgrAI    SnaBI     SphI     SrfI     SspI
     SwaI     TstI     XbaI     XhoI     XmnI
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