Restriction map for pF1KSDA1281
 (Linear) (Six-base) MAPSORT of: KIAA1281.seq  Check: 7432  from: 1  to: 4536



KIAA1281.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    4385    4473    4536 
   Size:       4385      88      63

  Fragments arranged by size: 

                4385      88      63
 
AccI GT'mk_AC

Cuts at:      0    3680    3737    4536 
   Size:       3680      57     799

  Fragments arranged by size: 

                3680     799      57
 
Acc65I G'GTAC_C

Cuts at:      0    1712    4536 
   Size:       1712    2824
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     637    3562    4536 
   Size:        637    2925     974

  Fragments arranged by size: 

                2925     974     637
 
AfeI AGC'GCT

Cuts at:      0     455    4536 
   Size:        455    4081
 
AflIII A'CryG_T

Cuts at:      0    1060    4536 
   Size:       1060    3476
 
AgeI A'CCGG_T

Cuts at:      0    1014    4536 
   Size:       1014    3522
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2859    3273    4536 
   Size:       2859     414    1263

  Fragments arranged by size: 

                2859    1263     414
 
AleI CACnn'nnGTG

Cuts at:      0    2725    4536 
   Size:       2725    1811
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1839    3059    4505    4536 
   Size:       1839    1220    1446      31

  Fragments arranged by size: 

                1839    1446    1220      31
 
AlwNI CAG_nnn'CTG

Cuts at:      0    3339    4536 
   Size:       3339    1197
 
ApaI G_GGCC'C

Cuts at:      0    1103    1242    4536 
   Size:       1103     139    3294

  Fragments arranged by size: 

                3294    1103     139
 
ApoI r'AATT_y

Cuts at:      0      93     139     250     285     447    1125    1575    1974 
   Size:         93      46     111      35     162     678     450     399

Cuts at:   1974    2127    4536 
   Size:        153    2409

  Fragments arranged by size: 

                2409     678     450     399     162     153     111      93
                  46      35
 
AseI AT'TA_AT

Cuts at:      0    2113    4536 
   Size:       2113    2423
 
AvaI C'yCGr_G

Cuts at:      0     382    1332    2703    3690    3974    4425    4536 
   Size:        382     950    1371     987     284     451     111

  Fragments arranged by size: 

                1371     987     950     451     382     284     111
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1829    1862    4536 
   Size:       1829      33    2674

  Fragments arranged by size: 

                2674    1829      33
 
BamHI G'GATC_C

Cuts at:      0      36    4536 
   Size:         36    4500
 
BanI G'GyrC_C

Cuts at:      0     422     598     698    1712    1869    2739    2748    3043 
   Size:        422     176     100    1014     157     870       9     295

Cuts at:   3043    4373    4536 
   Size:       1330     163

  Fragments arranged by size: 

                1330    1014     870     422     295     176     163     157
                 100       9
 
BanII G_rGCy'C

Cuts at:      0     745     824    1103    1242    1339    2393    3260    3398 
   Size:        745      79     279     139      97    1054     867     138

Cuts at:   3398    3884    4536 
   Size:        486     652

  Fragments arranged by size: 

                1054     867     745     652     486     279     139     138
                  97      79
 
BbeI G_GCGC'C

Cuts at:      0     426     602    4536 
   Size:        426     176    3934

  Fragments arranged by size: 

                3934     426     176
 
BbvCI CC'TCA_GC

Cuts at:      0    1460    2642    3182    4536 
   Size:       1460    1182     540    1354

  Fragments arranged by size: 

                1460    1354    1182     540
 
BclI T'GATC_A

Cuts at:      0    3197    4536 
   Size:       3197    1339
 
BfrBI ATG'CAT

Cuts at:      0    3250    4536 
   Size:       3250    1286
 
BglII A'GATC_T

Cuts at:      0    1781    4536 
   Size:       1781    2755
 
BlpI GC'TnA_GC

Cuts at:      0    3599    4316    4536 
   Size:       3599     717     220

  Fragments arranged by size: 

                3599     717     220
 
Bme1580I G_kGCm'C

Cuts at:      0     701     763     810    1103    1242    2205    3048    3845 
   Size:        701      62      47     293     139     963     843     797

Cuts at:   3845    4536 
   Size:        691

  Fragments arranged by size: 

                 963     843     797     701     691     293     139      62
                  47
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     773    1245    2294    3374    4536 
   Size:        773     472    1049    1080    1162

  Fragments arranged by size: 

                1162    1080    1049     773     472
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3836    4536 
   Size:       3836     700
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     449    1297    1518    1631    1774    4121    4240 
   Size:        192     257     848     221     113     143    2347     119

Cuts at:   4240    4536 
   Size:        296

  Fragments arranged by size: 

                2347     848     296     257     221     192     143     119
                 113
 
Bpu10I CC'TnA_GC

Cuts at:      0     234    1460    2642    3182    4536 
   Size:        234    1226    1182     540    1354

  Fragments arranged by size: 

                1354    1226    1182     540     234
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1586    4536 
   Size:       1586    2950
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     292    4536 
   Size:        292    4244
 
BsaAI yAC'GTr

Cuts at:      0    1184    1203    4536 
   Size:       1184      19    3333

  Fragments arranged by size: 

                3333    1184      19
 
BsaHI Gr'CG_yC

Cuts at:      0     423     599    4536 
   Size:        423     176    3937

  Fragments arranged by size: 

                3937     423     176
 
BsaWI w'CCGG_w

Cuts at:      0    1014    3477    4536 
   Size:       1014    2463    1059

  Fragments arranged by size: 

                2463    1059    1014
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1516    1546    4536 
   Size:       1516      30    2990

  Fragments arranged by size: 

                2990    1516      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     580     871     874     877     880    3386    3547    3892 
   Size:        580     291       3       3       3    2506     161     345

Cuts at:   3892    3934    3987    4536 
   Size:         42      53     549

  Fragments arranged by size: 

                2506     580     549     345     291     161      53      42
                   3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     456    1966    2223    2406    2505    2680    3411    3844 
   Size:        456    1510     257     183      99     175     731     433

Cuts at:   3844    4069    4536 
   Size:        225     467

  Fragments arranged by size: 

                1510     731     467     456     433     257     225     183
                 175      99
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1206    4363    4536 
   Size:       1206    3157     173

  Fragments arranged by size: 

                3157    1206     173
 
BsiHKAI G_wGCw'C

Cuts at:      0    3073    3398    4536 
   Size:       3073     325    1138

  Fragments arranged by size: 

                3073    1138     325
 
BsiWI C'GTAC_G

Cuts at:      0    1199    4536 
   Size:       1199    3337
 
BsmI GAATG_Cn'

Cuts at:      0    1828    2719    2934    4536 
   Size:       1828     891     215    1602

  Fragments arranged by size: 

                1828    1602     891     215
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2470    4536 
   Size:       2470    2066
 
Bsp1286I G_dGCh'C

Cuts at:      0     701     745     763     810     824    1103    1242    1339 
   Size:        701      44      18      47      14     279     139      97

Cuts at:   1339    2205    2393    3048    3073    3260    3398    3845    3884 
   Size:        866     188     655      25     187     138     447      39

Cuts at:   3884    4536 
   Size:        652

  Fragments arranged by size: 

                 866     701     655     652     447     279     188     187
                 139     138      97      47      44      39      25      18
                  14
 
BspEI T'CCGG_A

Cuts at:      0    3477    4536 
   Size:       3477    1059
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     656    2849    3027    4385    4473    4536 
   Size:        656    2193     178    1358      88      63

  Fragments arranged by size: 

                2193    1358     656     178      88      63
 
BsrBI CCG'CTC

Cuts at:      0    4320    4536 
   Size:       4320     216
 
BsrDI GCAATG_nn'

Cuts at:      0     732    1941    2210    4536 
   Size:        732    1209     269    2326

  Fragments arranged by size: 

                2326    1209     732     269
 
BsrFI r'CCGG_y

Cuts at:      0    1014    1872    4536 
   Size:       1014     858    2664

  Fragments arranged by size: 

                2664    1014     858
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     648    1318    4536 
   Size:        648     670    3218

  Fragments arranged by size: 

                3218     670     648
 
BstEII G'GTnAC_C

Cuts at:      0    4411    4536 
   Size:       4411     125
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     667    2382    3753    4536 
   Size:        667    1715    1371     783

  Fragments arranged by size: 

                1715    1371     783     667
 
BstYI r'GATC_y

Cuts at:      0      36    1781    4536 
   Size:         36    1745    2755

  Fragments arranged by size: 

                2755    1745      36
 
BstZ17I GTA'TAC

Cuts at:      0    3738    4536 
   Size:       3738     798
 
Bsu36I CC'TnA_GG

Cuts at:      0    1708    4536 
   Size:       1708    2828
 
BtgI C'CryG_G

Cuts at:      0     533    4536 
   Size:        533    4003
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      83    4536 
   Size:         83    4453
 
BtsI GCAGTG_nn'

Cuts at:      0     678     750    1833    2258    4536 
   Size:        678      72    1083     425    2278

  Fragments arranged by size: 

                2278    1083     678     425      72
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     174     209     714     749    4536 
   Size:        174      35     505      35    3787

  Fragments arranged by size: 

                3787     505     174      35      35
 
DraI TTT'AAA

Cuts at:      0    2561    3699    4536 
   Size:       2561    1138     837

  Fragments arranged by size: 

                2561    1138     837
 
EaeI y'GGCC_r

Cuts at:      0    2627    3240    3284    3449    4365    4536 
   Size:       2627     613      44     165     916     171

  Fragments arranged by size: 

                2627     916     613     171     165      44
 
EarI CTCTTCn'nnn_

Cuts at:      0     848    1300    1647    4175    4524    4536 
   Size:        848     452     347    2528     349      12

  Fragments arranged by size: 

                2528     848     452     349     347      12
 
EcoICRI GAG'CTC

Cuts at:      0    3396    4536 
   Size:       3396    1140
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     449     637    1297    1518    1631    1774    3562 
   Size:        192     257     188     660     221     113     143    1788

Cuts at:   3562    4121    4240    4536 
   Size:        559     119     296

  Fragments arranged by size: 

                1788     660     559     296     257     221     192     188
                 143     119     113
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     652    2690    4445    4536 
   Size:        652    2038    1755      91

  Fragments arranged by size: 

                2038    1755     652      91
 
EcoO109I rG'GnC_Cy

Cuts at:      0     684    1100    1239    1904    1992    4536 
   Size:        684     416     139     665      88    2544

  Fragments arranged by size: 

                2544     684     665     416     139      88
 
EcoRI G'AATT_C

Cuts at:      0     285     447    1974    4536 
   Size:        285     162    1527    2562

  Fragments arranged by size: 

                2562    1527     285     162
 
EcoRV GAT'ATC

Cuts at:      0    1467    4536 
   Size:       1467    3069
 
HaeII r_GCGC'y

Cuts at:      0     426     457     602     934    2702    4536 
   Size:        426      31     145     332    1768    1834

  Fragments arranged by size: 

                1834    1768     426     332     145      31
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1516    1548    1768    1800    2041    2073    2378    2410 
   Size:       1516      32     220      32     241      32     305      32

Cuts at:   2410    2845    2877    3259    3291    3673    3705    3895    3927 
   Size:        435      32     382      32     382      32     190      32

Cuts at:   3927    3977    3999    4009    4031    4339    4371    4536 
   Size:         50      22      10      22     308      32     165

  Fragments arranged by size: 

                1516     435     382     382     308     305     241     220
                 190     165      50      32      32      32      32      32
                  32      32      32      32      22      22      10
 
HincII GTy'rAC

Cuts at:      0     909    2765    3863    4215    4536 
   Size:        909    1856    1098     352     321

  Fragments arranged by size: 

                1856    1098     909     352     321
 
HpaI GTT'AAC

Cuts at:      0    3863    4536 
   Size:       3863     673
 
KasI G'GCGC_C

Cuts at:      0     422     598    4536 
   Size:        422     176    3938

  Fragments arranged by size: 

                3938     422     176
 
KpnI G_GTAC'C

Cuts at:      0    1716    4536 
   Size:       1716    2820
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      69    1050    2054    2585    2843    4536 
   Size:         69     981    1004     531     258    1693

  Fragments arranged by size: 

                1693    1004     981     531     258      69
 
MscI TGG'CCA

Cuts at:      0    2629    3242    3286    4536 
   Size:       2629     613      44    1250

  Fragments arranged by size: 

                2629    1250     613      44
 
MslI CAynn'nnrTG

Cuts at:      0     665     704    1821    2380    2398    2725    2996    3731 
   Size:        665      39    1117     559      18     327     271     735

Cuts at:   3731    4536 
   Size:        805

  Fragments arranged by size: 

                1117     805     735     665     559     327     271      39
                  18
 
MspA1I CmG'CkG

Cuts at:      0     476     485     535     784    1430    2791    3055    4315 
   Size:        476       9      50     249     646    1361     264    1260

Cuts at:   4315    4371    4536 
   Size:         56     165

  Fragments arranged by size: 

                1361    1260     646     476     264     249     165      56
                  50       9
 
NarI GG'CG_CC

Cuts at:      0     423     599    4536 
   Size:        423     176    3937

  Fragments arranged by size: 

                3937     423     176
 
NdeI CA'TA_TG

Cuts at:      0    3252    4536 
   Size:       3252    1284
 
NsiI A_TGCA'T

Cuts at:      0    3252    4536 
   Size:       3252    1284
 
NspI r_CATG'y

Cuts at:      0    1064    2761    3027    4042    4267    4536 
   Size:       1064    1697     266    1015     225     269

  Fragments arranged by size: 

                1697    1064    1015     269     266     225
 
PasI CC'CwG_GG

Cuts at:      0     279    3031    4536 
   Size:        279    2752    1505

  Fragments arranged by size: 

                2752    1505     279
 
PciI A'CATG_T

Cuts at:      0    1060    4536 
   Size:       1060    3476
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3339    4536 
   Size:       3339    1197
 
PfoI T'CCnGG_A

Cuts at:      0     628    4137    4518    4536 
   Size:        628    3509     381      18

  Fragments arranged by size: 

                3509     628     381      18
 
PmlI CAC'GTG

Cuts at:      0    1184    4536 
   Size:       1184    3352
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1522    1554    4536 
   Size:       1522      32    2982

  Fragments arranged by size: 

                2982    1522      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1904    4536 
   Size:       1904    2632
 
PspOMI G'GGCC_C

Cuts at:      0    1099    1238    4536 
   Size:       1099     139    3298

  Fragments arranged by size: 

                3298    1099     139
 
PspXI vC'TCGA_Gb

Cuts at:      0    2703    4536 
   Size:       2703    1833
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1183    1215    4536 
   Size:       1183      32    3321

  Fragments arranged by size: 

                3321    1183      32
 
PstI C_TGCA'G

Cuts at:      0      24     789    1373    3054    4536 
   Size:         24     765     584    1681    1482

  Fragments arranged by size: 

                1681    1482     765     584      24
 
PvuII CAG'CTG

Cuts at:      0    2791    3055    4315    4536 
   Size:       2791     264    1260     221

  Fragments arranged by size: 

                2791    1260     264     221
 
SacI G_AGCT'C

Cuts at:      0    3398    4536 
   Size:       3398    1138
 
SacII CC_GC'GG

Cuts at:      0     536    4536 
   Size:        536    4000
 
SanDI GG'GwC_CC

Cuts at:      0    1904    4536 
   Size:       1904    2632
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1647    4536 
   Size:       1647    2889
 
ScaI AGT'ACT

Cuts at:      0    3322    4033    4536 
   Size:       3322     711     503

  Fragments arranged by size: 

                3322     711     503
 
SfcI C'TryA_G

Cuts at:      0      20     785    1369    2945    3050    3300    3635    4310 
   Size:         20     765     584    1576     105     250     335     675

Cuts at:   4310    4536 
   Size:        226

  Fragments arranged by size: 

                1576     765     675     584     335     250     226     105
                  20
 
SfoI GGC'GCC

Cuts at:      0     424     600    4536 
   Size:        424     176    3936

  Fragments arranged by size: 

                3936     424     176
 
SmaI CCC'GGG

Cuts at:      0    1334    4427    4536 
   Size:       1334    3093     109

  Fragments arranged by size: 

                3093    1334     109
 
SmlI C'TyrA_G

Cuts at:      0    1601    2703    3690    4536 
   Size:       1601    1102     987     846

  Fragments arranged by size: 

                1601    1102     987     846
 
SpeI A'CTAG_T

Cuts at:      0     169    4536 
   Size:        169    4367
 
SphI G_CATG'C

Cuts at:      0    4042    4267    4536 
   Size:       4042     225     269

  Fragments arranged by size: 

                4042     269     225
 
SspI AAT'ATT

Cuts at:      0    1005    4536 
   Size:       1005    3531
 
StuI AGG'CCT

Cuts at:      0     268    4536 
   Size:        268    4268
 
StyI C'CwwG_G

Cuts at:      0     185     417    1632    2525    4536 
   Size:        185     232    1215     893    2011

  Fragments arranged by size: 

                2011    1215     893     232     185
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1039    1685    4017    4411    4536 
   Size:       1039     646    2332     394     125

  Fragments arranged by size: 

                2332    1039     646     394     125
 
TatI w'GTAC_w

Cuts at:      0    1688    3320    4031    4536 
   Size:       1688    1632     711     505

  Fragments arranged by size: 

                1688    1632     711     505
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      17      49    3971    4003    4536 
   Size:         17      32    3922      32     533

  Fragments arranged by size: 

                3922     533      32      32      17
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     424     670    1125    3250    4399    4536 
   Size:        424     246     455    2125    1149     137

  Fragments arranged by size: 

                2125    1149     455     424     246     137
 
XhoI C'TCGA_G

Cuts at:      0    2703    3690    4536 
   Size:       2703     987     846

  Fragments arranged by size: 

                2703     987     846
 
XmaI C'CCGG_G

Cuts at:      0    1332    4425    4536 
   Size:       1332    3093     111

  Fragments arranged by size: 

                3093    1332     111
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI    AlwNI     ApaI     ApoI     AseI     AvaI     BaeI
    BamHI     BanI    BanII     BbeI    BbvCI     BclI    BfrBI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrFI   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI    CspCI     DraI     EaeI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV    HaeII    Hin4I   HincII     HpaI
     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NarI     NdeI
     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmlI     PpiI
    PpuMI   PspOMI    PspXI     PsrI     PstI    PvuII     SacI    SacII
    SanDI     SapI     ScaI     SfcI     SfoI     SmaI     SmlI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI     XcmI
     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AclI    AflII     AloI    ApaLI     AscI    AsiSI    AvrII
     BbsI     BcgI    BciVI     BglI    BmgBI     BmtI    BsaBI    BsiEI
    BspHI    BsrGI   BssHII    BssSI    BstBI     ClaI   DraIII     DrdI
     EagI     EciI     FalI     FseI     FspI    FspAI  HindIII     MfeI
     MluI     NaeI     NcoI   NgoMIV     NheI     NotI     NruI     PacI
     PmeI    PshAI     PsiI     PvuI    RsrII     SalI     SbfI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SrfI     SwaI  Tth111I     XbaI
     XmnI     ZraI
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