Restriction map for pF1KSDA1267
 (Linear) (Six-base) MAPSORT of: KIAA1267.seq  Check: 3531  from: 1  to: 3315



KIAA1267.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AccI GT'mk_AC

Cuts at:      0    1603    3315 
   Size:       1603    1712
 
Acc65I G'GTAC_C

Cuts at:      0    1452    3315 
   Size:       1452    1863
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     288    1070    1073    1339    1639    2668    2713 
   Size:         59     229     782       3     266     300    1029      45

Cuts at:   2713    3315 
   Size:        602

  Fragments arranged by size: 

                1029     782     602     300     266     229      59      45
                   3
 
AfeI AGC'GCT

Cuts at:      0     910    2308    3315 
   Size:        910    1398    1007

  Fragments arranged by size: 

                1398    1007     910
 
AflII C'TTAA_G

Cuts at:      0    2113    3315 
   Size:       2113    1202
 
AflIII A'CryG_T

Cuts at:      0    1777    3315 
   Size:       1777    1538
 
AleI CACnn'nnGTG

Cuts at:      0    1762    2809    3010    3315 
   Size:       1762    1047     201     305

  Fragments arranged by size: 

                1762    1047     305     201
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3201    3315 
   Size:       3201     114
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3077    3109    3315 
   Size:       3077      32     206

  Fragments arranged by size: 

                3077     206      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      37    1343    2691    3315 
   Size:         37    1306    1348     624

  Fragments arranged by size: 

                1348    1306     624      37
 
ApoI r'AATT_y

Cuts at:      0    1385    1940    3315 
   Size:       1385     555    1375

  Fragments arranged by size: 

                1385    1375     555
 
AvaI C'yCGr_G

Cuts at:      0    1267    3017    3315 
   Size:       1267    1750     298

  Fragments arranged by size: 

                1750    1267     298
 
AvrII C'CTAG_G

Cuts at:      0    2965    3315 
   Size:       2965     350
 
BamHI G'GATC_C

Cuts at:      0     171    3315 
   Size:        171    3144
 
BanI G'GyrC_C

Cuts at:      0      12    1452    1567    1756    3315 
   Size:         12    1440     115     189    1559

  Fragments arranged by size: 

                1559    1440     189     115      12
 
BanII G_rGCy'C

Cuts at:      0     546    2575    2974    3315 
   Size:        546    2029     399     341

  Fragments arranged by size: 

                2029     546     399     341
 
BbeI G_GCGC'C

Cuts at:      0      16    3315 
   Size:         16    3299
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     215     294     803     985    1513    3315 
   Size:        215      79     509     182     528    1802

  Fragments arranged by size: 

                1802     528     509     215     182      79
 
BbvCI CC'TCA_GC

Cuts at:      0    1700    3315 
   Size:       1700    1615
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3042    3315 
   Size:       3042     273
 
BclI T'GATC_A

Cuts at:      0     504    3315 
   Size:        504    2811
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2667    3315 
   Size:       2667     648
 
BlpI GC'TnA_GC

Cuts at:      0     365    3296    3315 
   Size:        365    2931      19

  Fragments arranged by size: 

                2931     365      19
 
Bme1580I G_kGCm'C

Cuts at:      0     894    1572    2273    2790    3315 
   Size:        894     678     701     517     525

  Fragments arranged by size: 

                 894     701     678     525     517
 
BmgBI CAC'GTC

Cuts at:      0    1780    3315 
   Size:       1780    1535
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1971    3315 
   Size:       1971    1344
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     744    3315 
   Size:        744    2571
 
Bpu10I CC'TnA_GC

Cuts at:      0    1700    3315 
   Size:       1700    1615
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1032    1080    1191    3247    3315 
   Size:       1032      48     111    2056      68

  Fragments arranged by size: 

                2056    1032     111      68      48
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1008    1072    2360    3315 
   Size:       1008      64    1288     955

  Fragments arranged by size: 

                1288    1008     955      64
 
BsaAI yAC'GTr

Cuts at:      0    1420    3315 
   Size:       1420    1895
 
BsaBI GATnn'nnATC

Cuts at:      0     588    1886    3315 
   Size:        588    1298    1429

  Fragments arranged by size: 

                1429    1298     588
 
BsaHI Gr'CG_yC

Cuts at:      0      13    3315 
   Size:         13    3302
 
BsaWI w'CCGG_w

Cuts at:      0      53    1853    3315 
   Size:         53    1800    1462

  Fragments arranged by size: 

                1800    1462      53
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1917    1947    3231    3261    3315 
   Size:       1917      30    1284      30      54

  Fragments arranged by size: 

                1917    1284      54      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2475    2571    2725    2788    3315 
   Size:       2475      96     154      63     527

  Fragments arranged by size: 

                2475     527     154      96      63
 
BseYI C'CCAG_C

Cuts at:      0     175     960    2662    2819    2870    2874    2900    3072 
   Size:        175     785    1702     157      51       4      26     172

Cuts at:   3072    3315 
   Size:        243

  Fragments arranged by size: 

                1702     785     243     175     172     157      51      26
                   4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1197    2483    2974    3171    3315 
   Size:       1197    1286     491     197     144

  Fragments arranged by size: 

                1286    1197     491     197     144
 
BsiEI CG_ry'CG

Cuts at:      0    2859    3315 
   Size:       2859     456
 
BsiHKAI G_wGCw'C

Cuts at:      0    1623    2127    2575    3315 
   Size:       1623     504     448     740

  Fragments arranged by size: 

                1623     740     504     448
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1284    1743    3315 
   Size:       1284     459    1572

  Fragments arranged by size: 

                1572    1284     459
 
Bsp1286I G_dGCh'C

Cuts at:      0     546     894    1572    1623    2127    2273    2575    2790 
   Size:        546     348     678      51     504     146     302     215

Cuts at:   2790    2974    3315 
   Size:        184     341

  Fragments arranged by size: 

                 678     546     504     348     341     302     215     184
                 146      51
 
BspHI T'CATG_A

Cuts at:      0     507    3315 
   Size:        507    2808
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     924    1286    2825    3315 
   Size:        924     362    1539     490

  Fragments arranged by size: 

                1539     924     490     362
 
BsrBI CCG'CTC

Cuts at:      0      19    3108    3315 
   Size:         19    3089     207

  Fragments arranged by size: 

                3089     207      19
 
BsrDI GCAATG_nn'

Cuts at:      0     158    3315 
   Size:        158    3157
 
BsrFI r'CCGG_y

Cuts at:      0     717    3315 
   Size:        717    2598
 
BssSI C'ACGA_G

Cuts at:      0    2805    3315 
   Size:       2805     510
 
BstYI r'GATC_y

Cuts at:      0     171     583    3315 
   Size:        171     412    2732

  Fragments arranged by size: 

                2732     412     171
 
Bsu36I CC'TnA_GG

Cuts at:      0    3201    3315 
   Size:       3201     114
 
BtgI C'CryG_G

Cuts at:      0     707    1018    1563    2273    2952    3315 
   Size:        707     311     545     710     679     363

  Fragments arranged by size: 

                 710     707     679     545     363     311
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      22    2522    3315 
   Size:         22    2500     793

  Fragments arranged by size: 

                2500     793      22
 
BtsI GCAGTG_nn'

Cuts at:      0     102     680    1185    2886    3315 
   Size:        102     578     505    1701     429

  Fragments arranged by size: 

                1701     578     505     429     102
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1596    1631    3315 
   Size:       1596      35    1684

  Fragments arranged by size: 

                1684    1596      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1906    3315 
   Size:       1906    1409
 
EaeI y'GGCC_r

Cuts at:      0    2856    3216    3315 
   Size:       2856     360      99

  Fragments arranged by size: 

                2856     360      99
 
EagI C'GGCC_G

Cuts at:      0    2856    3315 
   Size:       2856     459
 
EarI CTCTTCn'nnn_

Cuts at:      0     566     632    1235    1658    1746    2714    3315 
   Size:        566      66     603     423      88     968     601

  Fragments arranged by size: 

                 968     603     601     566     423      88      66
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1858    3126    3157    3315 
   Size:       1858    1268      31     158

  Fragments arranged by size: 

                1858    1268     158      31
 
EcoICRI GAG'CTC

Cuts at:      0    2573    3315 
   Size:       2573     742
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     288     744    1070    1073    1339    1639    2668 
   Size:         59     229     456     326       3     266     300    1029

Cuts at:   2668    2713    3315 
   Size:         45     602

  Fragments arranged by size: 

                1029     602     456     326     300     266     229      59
                  45       3
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2234    2730    2837    3315 
   Size:       2234     496     107     478

  Fragments arranged by size: 

                2234     496     478     107
 
EcoRI G'AATT_C

Cuts at:      0    1385    3315 
   Size:       1385    1930
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     228    3315 
   Size:        228    3087
 
FspI TGC'GCA

Cuts at:      0    1899    3315 
   Size:       1899    1416
 
HaeII r_GCGC'y

Cuts at:      0      16     912    2296    2310    3238    3303    3315 
   Size:         16     896    1384      14     928      65      12

  Fragments arranged by size: 

                1384     928     896      65      16      14      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     247     279     748     780    1023    1055    1585    1617 
   Size:        247      32     469      32     243      32     530      32

Cuts at:   1617    1718    1750    2359    2391    2671    2703    2719    2751 
   Size:        101      32     609      32     280      32      16      32

Cuts at:   2751    3073    3105    3258    3290    3315 
   Size:        322      32     153      32      25

  Fragments arranged by size: 

                 609     530     469     322     280     247     243     153
                 101      32      32      32      32      32      32      32
                  32      32      32      25      16
 
KasI G'GCGC_C

Cuts at:      0      12    3315 
   Size:         12    3303
 
KpnI G_GTAC'C

Cuts at:      0    1456    3315 
   Size:       1456    1859
 
MfeI C'AATT_G

Cuts at:      0     618    3315 
   Size:        618    2697
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1943    1961    2762    3315 
   Size:       1943      18     801     553

  Fragments arranged by size: 

                1943     801     553      18
 
MslI CAynn'nnrTG

Cuts at:      0     569    1762    2410    2602    2809    3010    3315 
   Size:        569    1193     648     192     207     201     305

  Fragments arranged by size: 

                1193     648     569     305     207     201     192
 
MspA1I CmG'CkG

Cuts at:      0      37     960    1343    2666    2823    2874    3159    3185 
   Size:         37     923     383    1323     157      51     285      26

Cuts at:   3185    3315 
   Size:        130

  Fragments arranged by size: 

                1323     923     383     285     157     130      51      37
                  26
 
NaeI GCC'GGC

Cuts at:      0     719    3315 
   Size:        719    2596
 
NarI GG'CG_CC

Cuts at:      0      13    3315 
   Size:         13    3302
 
NcoI C'CATG_G

Cuts at:      0     707    1563    2273    2952    3315 
   Size:        707     856     710     679     363

  Fragments arranged by size: 

                 856     710     707     679     363
 
NgoMIV G'CCGG_C

Cuts at:      0     717    3315 
   Size:        717    2598
 
NspI r_CATG'y

Cuts at:      0    2052    2764    3315 
   Size:       2052     712     551

  Fragments arranged by size: 

                2052     712     551
 
PasI CC'CwG_GG

Cuts at:      0    3101    3315 
   Size:       3101     214
 
PpuMI rG'GwC_Cy

Cuts at:      0    2234    2730    2837    3315 
   Size:       2234     496     107     478

  Fragments arranged by size: 

                2234     496     478     107
 
PshAI GACnn'nnGTC

Cuts at:      0    1296    3315 
   Size:       1296    2019
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     251     283     408     440    3315 
   Size:        251      32     125      32    2875

  Fragments arranged by size: 

                2875     251     125      32      32
 
PstI C_TGCA'G

Cuts at:      0     168    1320    3315 
   Size:        168    1152    1995

  Fragments arranged by size: 

                1995    1152     168
 
PvuII CAG'CTG

Cuts at:      0      37     960    2666    2874    3159    3315 
   Size:         37     923    1706     208     285     156

  Fragments arranged by size: 

                1706     923     285     208     156      37
 
SacI G_AGCT'C

Cuts at:      0    2575    3315 
   Size:       2575     740
 
SapI GCTCTTCn'nnn_

Cuts at:      0     566    3315 
   Size:        566    2749
 
SexAI A'CCwGG_T

Cuts at:      0     747    3315 
   Size:        747    2568
 
SfcI C'TryA_G

Cuts at:      0     164    1316    2841    3315 
   Size:        164    1152    1525     474

  Fragments arranged by size: 

                1525    1152     474     164
 
SfoI GGC'GCC

Cuts at:      0      14    3315 
   Size:         14    3301
 
SmlI C'TyrA_G

Cuts at:      0    1011    1059    1170    2113    3262    3315 
   Size:       1011      48     111     943    1149      53

  Fragments arranged by size: 

                1149    1011     943     111      53      48
 
SphI G_CATG'C

Cuts at:      0    2052    2764    3315 
   Size:       2052     712     551

  Fragments arranged by size: 

                2052     712     551
 
StyI C'CwwG_G

Cuts at:      0     203     707    1563    2273    2462    2952    2965    3315 
   Size:        203     504     856     710     189     490      13     350

  Fragments arranged by size: 

                 856     710     504     490     350     203     189      13
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     952    2142    2455    3315 
   Size:        952    1190     313     860

  Fragments arranged by size: 

                1190     952     860     313
 
TatI w'GTAC_w

Cuts at:      0    1785    3315 
   Size:       1785    1530
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      35      67    1917    1949    2430    2462    2793    2825 
   Size:         35      32    1850      32     481      32     331      32

Cuts at:   2825    3231    3263    3315 
   Size:        406      32      52

  Fragments arranged by size: 

                1850     481     406     331      52      35      32      32
                  32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     210     768    3069    3315 
   Size:        210     558    2301     246

  Fragments arranged by size: 

                2301     558     246     210
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI    AflII   AflIII     AleI     AlfI
     AloI    AlwNI     ApoI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I     KasI     KpnI     MfeI     MmeI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI    PpuMI
    PshAI     PsrI     PstI    PvuII     SacI     SapI    SexAI     SfcI
     SfoI     SmlI     SphI     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AgeI     AhdI     ApaI    ApaLI     AscI
     AseI    AsiSI     BaeI     BcgI    BfrBI    BglII     BmtI     BplI
    BsiWI     BsmI    BspEI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I     ClaI     DraI     DrdI    EcoNI    EcoRV     FseI
    FspAI   HincII  HindIII     HpaI     MluI     MscI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI     PsiI   PspOMI    PspXI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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