Restriction map for pF1KSDA1263
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AciI        
AclI        AcyI        AflII       AgeI        AhdI        
AocI        Aor51HI     ApaI        AscI        AspEI       
AvaI        AviII       AvrII       BamHI       BbeI        
BbrPI       BcgI        BfrI        BglI        BglII       
BlnI        BsaAI       BsaBI       BsaHI       BseAI       
BsiEI       BsiWI       BsmBI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstUI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoRV       EcoT22I     FauI        FseI        
FspI        HaeII       HgaI        Hin1I       HincII      
HindII      HpaI        KasI        KspI        MamI        
MfeI        MluI        MroI        MunI        MvnI        
NaeI        NarI        NcoI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        TaqI        TthHB8I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I       1681  GGTACC               G^GTACC
AflIII       1352  ACATGT               A^C[GA][CT]GT
Alw44I        337  GTGCAC               G^TGCAC
ApaLI         337  GTGCAC               G^TGCAC
Asp700I      1350  GAACATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1681  GGTACC               G^GTACC
BanII        2305  GAGCTC               G[GA]GC[CT]^C
BciVI        1032  GTATCC               GTATCC(6/5)
BclI         1656  TGATCA               T^GATCA
BcnI         1026  CCGGG                CC^[GC]GG
BlpI         1671  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     1671  GCTAAGC              GC^T[ACGT]AGC
BsaI          825  GAGACC               GGTCTC(1/5)
BseRI        2168  GAGGAG               GAGGAG(10/8)
BsmI          718  GCATTC               GAATGC(1/-1)
BspLU11I     1352  ACATGT               A^CATGT
BspMI         955  ACCTGC               ACCTGC(4/8)
BsrDI        1199  GCAATG               GCAATG(2/0)
BstAPI        250  GCAGAATGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        927  GGTTACC              G^GT[ACGT]ACC
BstPI         927  GGTTACC              G^GT[ACGT]ACC
BstXI        2397  CCAGGACAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1671  GCTAAGC              GC^T[ACGT]AGC
CfoI         1206  GCGC                 GCG^C
DraII         907  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I      907  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       927  GGTTACC              G^GT[ACGT]ACC
EcoRI         313  GAATTC               G^AATTC
FbaI         1656  TGATCA               T^GATCA
HhaI         1206  GCGC                 GCG^C
HinP1I       1206  GCGC                 G^CGC
HindIII      1147  AAGCTT               A^AGCTT
KpnI         1681  GGTACC               GGTAC^C
MspA1I       1511  CAGCTG               C[AC]G^C[GT]G
NciI         1026  CCGGG                CC^[GC]GG
NspI         1352  ACATGT               [GA]CATG^[CT]
PpuMI         907  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI          770  CTGCAG               CTGCA^G
PvuII        1511  CAGCTG               CAG^CTG
SacI         2305  GAGCTC               GAGCT^C
ScaI          200  AGTACT               AGT^ACT
XcmI         1830  CCATATGAGGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1350  GAACATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwNI         773  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1616  CAGACCCTG           
AseI          586  ATTAAT               AT^TAAT
             2122  ATTAAT              
AsnI          586  ATTAAT               AT^TAAT
             2122  ATTAAT              
AvaII         267  GGACC                G^G[AT]CC
              908  GGTCC               
BalI         1724  TGGCCA               TGG^CCA
             2103  TGGCCA              
BanI         1079  GGTGCC               G^G[CT][GA]CC
             1681  GGTACC              
BbvCI        1105  CCTCAGC              CCTCAGC(-5/-2)
             1474  GCTGAGG             
BsaWI        2005  ACCGGA               [AT]^CCGG[AT]
             2378  ACCGGA              
Cac8I         246  GCAAGC               GC[ACGT]^[ACGT]GC
              349  GCTTGC              
DraI          400  TTTAAA               TTT^AAA
              547  TTTAAA              
EaeI         1724  TGGCCA               [CT]^GGCC[GA]
             2103  TGGCCA              
MaeII         279  ACGT                 A^CGT
              941  ACGT                
MluNI        1724  TGGCCA               TGG^CCA
             2103  TGGCCA              
MscI         1724  TGGCCA               TGG^CCA
             2103  TGGCCA              
NdeI         1831  CATATG               CA^TATG
             2087  CATATG              
PflMI         833  CCATTATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1761  CCAACCAGTGG         
PshBI         586  ATTAAT               AT^TAAT
             2122  ATTAAT              
SfcI          770  CTGCAG               C^T[GA][CT]AG
             1708  CTACAG              
SmlI         2341  CTCAAG               C^T[CT][GA]AG
             2353  CTCAAG              
SspI          551  AATATT               AAT^ATT
              853  AATATT              
Tsp45I        281  GTCAC                ^GT[GC]AC
             2074  GTGAC               
Van91I        833  CCATTATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1761  CCAACCAGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
AspHI       AspI        BbsI        BbvI        BfaI        
BmyI        BpmI        BpuAI       BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cfr13I      DdeI        DpnII       EarI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PflFI       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       Tth111I     XhoII       
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