Restriction map for pF1KSDA1263
 (Linear) (Six-base) MAPSORT of: KIAA1263.seq  Check: 9  from: 1  to: 2538



KIAA1263.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     964    2538 
   Size:        964    1574
 
Acc65I G'GTAC_C

Cuts at:      0    1681    2538 
   Size:       1681     857
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2264    2516    2538 
   Size:       2264     252      22

  Fragments arranged by size: 

                2264     252      22
 
AflIII A'CryG_T

Cuts at:      0    1352    2538 
   Size:       1352    1186
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     362    2538 
   Size:        362    2176
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1472    1504    2538 
   Size:       1472      32    1034

  Fragments arranged by size: 

                1472    1034      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     778    1621    2538 
   Size:        778     843     917

  Fragments arranged by size: 

                 917     843     778
 
ApaLI G'TGCA_C

Cuts at:      0     337    2538 
   Size:        337    2201
 
ApoI r'AATT_y

Cuts at:      0     313     706    1408    2538 
   Size:        313     393     702    1130

  Fragments arranged by size: 

                1130     702     393     313
 
AseI AT'TA_AT

Cuts at:      0     587    2123    2538 
   Size:        587    1536     415

  Fragments arranged by size: 

                1536     587     415
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1218    1251    2538 
   Size:       1218      33    1287

  Fragments arranged by size: 

                1287    1218      33
 
BanI G'GyrC_C

Cuts at:      0    1079    1681    2538 
   Size:       1079     602     857

  Fragments arranged by size: 

                1079     857     602
 
BanII G_rGCy'C

Cuts at:      0    2309    2538 
   Size:       2309     229
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     222     395     887    1271    1295    1699    2538 
   Size:        222     173     492     384      24     404     839

  Fragments arranged by size: 

                 839     492     404     384     222     173      24
 
BbvCI CC'TCA_GC

Cuts at:      0    1106    1475    2538 
   Size:       1106     369    1063

  Fragments arranged by size: 

                1106    1063     369
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1043    2538 
   Size:       1043    1495
 
BclI T'GATC_A

Cuts at:      0    1656    2538 
   Size:       1656     882
 
BlpI GC'TnA_GC

Cuts at:      0    1672    2538 
   Size:       1672     866
 
Bme1580I G_kGCm'C

Cuts at:      0     341    2083    2538 
   Size:        341    1742     455

  Fragments arranged by size: 

                1742     455     341
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1621    2538 
   Size:       1621     917
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67    1001    1241    2081    2538 
   Size:         67     934     240     840     457

  Fragments arranged by size: 

                 934     840     457     240      67
 
Bpu10I CC'TnA_GC

Cuts at:      0    1106    1475    2538 
   Size:       1106     369    1063

  Fragments arranged by size: 

                1106    1063     369
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2326    2338    2538 
   Size:       2326      12     200

  Fragments arranged by size: 

                2326     200      12
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     819    2538 
   Size:        819    1719
 
BsaWI w'CCGG_w

Cuts at:      0    2005    2378    2538 
   Size:       2005     373     160

  Fragments arranged by size: 

                2005     373     160
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1472    1502    2067    2097    2538 
   Size:       1472      30     565      30     441

  Fragments arranged by size: 

                1472     565     441      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2183    2538 
   Size:       2183     355
 
BseYI C'CCAG_C

Cuts at:      0    1676    2538 
   Size:       1676     862
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1603    1853    2191    2538 
   Size:       1603     250     338     347

  Fragments arranged by size: 

                1603     347     338     250
 
BsiHKAI G_wGCw'C

Cuts at:      0     341    1615    2297    2309    2538 
   Size:        341    1274     682      12     229

  Fragments arranged by size: 

                1274     682     341     229      12
 
BsmI GAATG_Cn'

Cuts at:      0     718    2538 
   Size:        718    1820
 
Bsp1286I G_dGCh'C

Cuts at:      0     341    1615    2083    2297    2309    2538 
   Size:        341    1274     468     214      12     229

  Fragments arranged by size: 

                1274     468     341     229     214      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     964    2538 
   Size:        964    1574
 
BsrDI GCAATG_nn'

Cuts at:      0    1206    2538 
   Size:       1206    1332
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     256    2538 
   Size:        256    2282
 
BstEII G'GTnAC_C

Cuts at:      0     927    2538 
   Size:        927    1611
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2404    2538 
   Size:       2404     134
 
BstYI r'GATC_y

Cuts at:      0     184     301     630    2538 
   Size:        184     117     329    1908

  Fragments arranged by size: 

                1908     329     184     117
 
BtsI GCAGTG_nn'

Cuts at:      0     767    1188    1613    2538 
   Size:        767     421     425     925

  Fragments arranged by size: 

                 925     767     425     421
 
DraI TTT'AAA

Cuts at:      0     402     549    2538 
   Size:        402     147    1989

  Fragments arranged by size: 

                1989     402     147
 
EaeI y'GGCC_r

Cuts at:      0    1724    2103    2538 
   Size:       1724     379     435

  Fragments arranged by size: 

                1724     435     379
 
EarI CTCTTCn'nnn_

Cuts at:      0    1278    2316    2455    2538 
   Size:       1278    1038     139      83

  Fragments arranged by size: 

                1278    1038     139      83
 
EcoICRI GAG'CTC

Cuts at:      0    2307    2538 
   Size:       2307     231
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67    1001    1241    2081    2264    2516    2538 
   Size:         67     934     240     840     183     252      22

  Fragments arranged by size: 

                 934     840     252     240     183      67      22
 
EcoO109I rG'GnC_Cy

Cuts at:      0     908    2538 
   Size:        908    1630
 
EcoRI G'AATT_C

Cuts at:      0     313    2538 
   Size:        313    2225
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     651    2538 
   Size:        651    1887
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     499     531     892     924    1570    1602    1681    1713 
   Size:        499      32     361      32     646      32      79      32

Cuts at:   1713    2067    2099    2322    2354    2538 
   Size:        354      32     223      32     184

  Fragments arranged by size: 

                 646     499     361     354     223     184      79      32
                  32      32      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1147    2538 
   Size:       1147    1391
 
KpnI G_GTAC'C

Cuts at:      0    1685    2538 
   Size:       1685     853
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      32    2508    2538 
   Size:         32    2476      30

  Fragments arranged by size: 

                2476      32      30
 
MscI TGG'CCA

Cuts at:      0    1726    2105    2538 
   Size:       1726     379     433

  Fragments arranged by size: 

                1726     433     379
 
MslI CAynn'nnrTG

Cuts at:      0    1027    1831    1859    2538 
   Size:       1027     804      28     679

  Fragments arranged by size: 

                1027     804     679      28
 
MspA1I CmG'CkG

Cuts at:      0    1513    2538 
   Size:       1513    1025
 
NdeI CA'TA_TG

Cuts at:      0    1832    2088    2538 
   Size:       1832     256     450

  Fragments arranged by size: 

                1832     450     256
 
NspI r_CATG'y

Cuts at:      0    1356    2538 
   Size:       1356    1182
 
PciI A'CATG_T

Cuts at:      0    1352    2538 
   Size:       1352    1186
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     839    1767    2538 
   Size:        839     928     771

  Fragments arranged by size: 

                 928     839     771
 
PfoI T'CCnGG_A

Cuts at:      0    2396    2538 
   Size:       2396     142
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1472    1504    2538 
   Size:       1472      32    1034

  Fragments arranged by size: 

                1472    1034      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     908    2538 
   Size:        908    1630
 
PstI C_TGCA'G

Cuts at:      0     774    2538 
   Size:        774    1764
 
PvuII CAG'CTG

Cuts at:      0    1513    2538 
   Size:       1513    1025
 
SacI G_AGCT'C

Cuts at:      0    2309    2538 
   Size:       2309     229
 
ScaI AGT'ACT

Cuts at:      0     202    2538 
   Size:        202    2336
 
SfcI C'TryA_G

Cuts at:      0     770    1708    2538 
   Size:        770     938     830

  Fragments arranged by size: 

                 938     830     770
 
SmlI C'TyrA_G

Cuts at:      0    2341    2353    2538 
   Size:       2341      12     185

  Fragments arranged by size: 

                2341     185      12
 
SspI AAT'ATT

Cuts at:      0     553     855    2538 
   Size:        553     302    1683

  Fragments arranged by size: 

                1683     553     302
 
StyI C'CwwG_G

Cuts at:      0    1443    1685    2371    2438    2538 
   Size:       1443     242     686      67     100

  Fragments arranged by size: 

                1443     686     242     100      67
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1656    1774    1843    2538 
   Size:       1656     118      69     695

  Fragments arranged by size: 

                1656     695     118      69
 
TatI w'GTAC_w

Cuts at:      0     200     643    2153    2538 
   Size:        200     443    1510     385

  Fragments arranged by size: 

                1510     443     385     200
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1370    1402    1918    1950    2538 
   Size:       1370      32     516      32     588

  Fragments arranged by size: 

                1370     588     516      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1564    1621    2050    2538 
   Size:       1564      57     429     488

  Fragments arranged by size: 

                1564     488     429      57
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1837    2538 
   Size:       1837     701
 
XmnI GAAnn'nnTTC

Cuts at:      0    1354    2538 
   Size:       1354    1184
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII     AlfI     AloI    AlwNI    ApaLI
     ApoI     AseI     BaeI     BanI    BanII     BbsI    BbvCI    BciVI
     BclI     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrDI   BstAPI   BstEII    BstXI    BstYI     BtsI     DraI
     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI    Hin4I
  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NdeI     NspI
     PciI    PflMI     PfoI     PpiI    PpuMI     PstI    PvuII     SacI
     ScaI     SfcI     SmlI     SspI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI     AleI
     ApaI     AscI    AsiSI     AvaI    AvrII    BamHI     BbeI     BcgI
    BfrBI     BglI    BglII    BmgBI     BmtI     BplI    BsaAI    BsaBI
    BsaHI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI    BstBI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI   DraIII     DrdI     EagI     EciI    EcoNI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     MfeI
     MluI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PmeI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI     XbaI     XhoI     XmaI
     ZraI
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